FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-6_S47_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-6_S47_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086
Sequences flagged as poor quality0
Sequence length84-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC292.6703499079189688No Hit
ATTCTGAGATTCATTTCACTATTTGAATGTTGTCTATTAATGTTTACATT80.7366482504604052No Hit
AAGAGATAGATGCAATTCTCCAGTGCCTGCTACAAGTTGTGTTTAAATTG80.7366482504604052No Hit
CAGCCATTCACGCAGAAGCTCCTCCATTTGTGGAAATGAGTGTGAAACAA70.6445672191528545No Hit
CGATTTTGCTTTTTTTTCTTCATTTTAAAGCATATTTGTTATGAATGTAT70.6445672191528545No Hit
GGAAGGTACATTTTTGGGATGTCATACCTCACTTGTCGTGCGAGGGTTAC60.5524861878453038No Hit
AGAAGGTCGAGTGCCACCTGTGTTTCAAGTCATACCGATGATGAACATGT60.5524861878453038No Hit
GGCCTACCACGAGCAGCTCTCCGTTGCCGAAATCACCAACGCTTGCTTCG60.5524861878453038No Hit
CGTTATGGTGGTTACAAAGGATATAGAAAAGGAGGAAAATATTTTTGAAC60.5524861878453038No Hit
CGAGAACAAGCAGCCAGGGACCAGGAAGCCCTGCTCAGTGCTATGTCTGC60.5524861878453038No Hit
AGAAGGGCATCTTGTTTGTATATGTAGAACGACCATTTTTTTATAATAAA60.5524861878453038No Hit
GCTATGTTCATGTCTGGGCTTGTACACAGAGTTTGTGTTACAACATGTTT60.5524861878453038No Hit
GTTCCTTGACACTAGCTGCATATTATCTAACTTTGTTTCTACAGTTTTAT60.5524861878453038No Hit
TTCTAATGTCTACATTTTTCTTCCGATCTTCATGAAACTTAAATCTTAGT60.5524861878453038No Hit
CTCTTATGCATGAAGACGGAAGCACACGTGACGATTTGAAAGTGCCAGAT60.5524861878453038No Hit
CTTTGTCGTATCCTATATCACATTGACCTCTCACATTGACATGTCTCACA50.46040515653775327No Hit
GCCGTGTTGTTATCTTCAGGGGTGAGTGTGACGTGAAGTCATTATGCGAT50.46040515653775327No Hit
CTTAACGCCAGCCTAAGAATACAACGTTCGATATAATCATTTGACATTTT50.46040515653775327No Hit
TGGCCAGGTCTGTTTGCCCGTTCTCTTGAAGGAGTAAATGTGAAAGAACT50.46040515653775327No Hit
CACTAACCTCCCACTGCAGAGGTCTTTGTTTCGTCTGTTTTATTACAACA50.46040515653775327No Hit
AAATATTCCACAAGATGTTGATGCCCAAGAAGAATCGAGTGTCTATTTAT40.3683241252302026No Hit
GCGGGTTTGATGTACAGCATGCCAGTAATTATTGATAAGCAGAAGAAATG40.3683241252302026No Hit
GGTGATGCAGGCGCTGGTGGTGCTGCCCCAGGTGGCGGTTCCAGTGGTGG40.3683241252302026No Hit
CTTACATAAAAATTATTACATTTAAGTCTATTTATGTTTAATTATTTTAT40.3683241252302026No Hit
GTAAGTATGAAGTTGAGTTTGTGTAGCCGCCTGCTCATTTCAGTTTTCTG40.3683241252302026No Hit
CCAGATTATTGGAAAGATCCAGAATCTGAACCTTTCAGAAAATTGTTTAT40.3683241252302026No Hit
GTTTGAAGTGGGACGATAATAATTGTGAACTGAAATTCAACCCATTATGC40.3683241252302026No Hit
AAAAAGCGCAGTGCTTTATGGGGCTTGGTGGTTACAGTAGTTCTTGGTGG40.3683241252302026No Hit
GTAAAGTACCTGCCCGTGTCGTTGCCAAAGATATTGGCGGTGACAAGAAT40.3683241252302026No Hit
GCTTTCAACACTCGCATTCAGAAATGGTGGTGTTCGGGCTTTGAAGTCGT40.3683241252302026No Hit
CTCATTCCCAGTTTATTTTTTATCAATACCATCTGATATGATGTTGTCTA40.3683241252302026No Hit
ATTTCAGGTGTTCAGTTTTGAAAATAGTATATTCTCAGATTGAACTTTAT40.3683241252302026No Hit
TGTACCCCGGCATCGCTGACAGAATGCAGAAGGAAATAACATCCCTTGCT40.3683241252302026No Hit
GATTAAACCACTCGTGCTTAGATTTACAAAACAAGTCATTTTTTTTAAAA40.3683241252302026No Hit
CGAAAATGGATTGAAAAATGTACTAGTAATTTTAAGACCATTAACTATTT40.3683241252302026No Hit
GTACTTAATAAGGTTTGTAAATTGATTTATTATTGTAAAAAAAAAATCTG40.3683241252302026No Hit
GGGAAATCTATTTTAGTTGAACATAGGCGCGTAGCCGAAGTTAGGAAATC40.3683241252302026No Hit
GGCAAATATGACTCTCCAGGGACAAATGGGCAGGACACAAAATGGCATTC40.3683241252302026No Hit
CTTTGATTAAGTCGGCCAGACAAGTAAAACAATGTCTTACAAGGACACTA40.3683241252302026No Hit
CCCAGATTATTTGTCGGAATGATTCTAATTCTTATTTTTGCTGAAGTATT40.3683241252302026No Hit
TGAAGGAACTCGGCGTACGTCTTGATGAAGCAGAAGCACAGGCTCTTAAG40.3683241252302026No Hit
TTCAAACCTGGATATTTTTTGTGAGAATTTGAAATGGACAAAAGAGAGAG40.3683241252302026No Hit
GCCATACCCAAAGTCAAGATTTTGTCGTGGTGTACCAGATCCAAAGATCC40.3683241252302026No Hit
CTGGAACGAATCGATGTATTACGAGAACTCAGACCAGAGTTTAGTATGGA40.3683241252302026No Hit
GCCAAAGCAAGCGACTCCCTTAGGACCAGAGAAGACCTCTTTCTTCCAGG30.2762430939226519No Hit
TGAAAATTAACTTGTTTTACATAATAATTGTATATTTATGTGGGATCCAG30.2762430939226519No Hit
TGACGACGTGTCACGCGTAAGACCTACATGTGTACCTCAAAGGTCATAGT30.2762430939226519No Hit
GGCCTGACATCGTAGCTTGGATTTGAGGAGGGTTTGCGGTAAGGGACGCA30.2762430939226519No Hit
GACGGAGATGGTACTGGTGATGTGGTAACCCGTAAACTCATGCAACTGGT30.2762430939226519No Hit
TATGGGAAAAATAGGAAACAAATGTAAAAGCCGATTTAATTTTTTTTAAG30.2762430939226519No Hit
TTTTGATATTTAAAGGAAAAACTCTTCAAATTGGAGGTAAGGGTTAGGGC30.2762430939226519No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC30.2762430939226519No Hit
TGAACGTTCACAAGAGAAGTACCTTGTGAAAATAAGAGAACCAAAGGGAT30.2762430939226519No Hit
TACTGGAATAACTATACCACATCTTATTTTGTAATATATGTGTTTCCCCG30.2762430939226519No Hit
CTAGAATATGCACATCTACATAGTAAATGAACCTATAATTAATGCTGACC30.2762430939226519No Hit
TAAAAATACATTTTATTTATTACATGCATGTATTTAATAATTGTGATGTT30.2762430939226519No Hit
CTTATTTTAAATGAGTGGATTAAGGCATTGTAATGGATAGAAACGAAGAT30.2762430939226519No Hit
CTTAGATATATTTAACATGTAGCATTGAATCGGGGTCCCCTAAAAGATTT30.2762430939226519No Hit
ATTTGGGAAAGGAATGCATACATTAATAACTATTTACGGTATAAAACACA30.2762430939226519No Hit
CTAAAGGAACCTATTGATTAGCATACAGAAGTGTAATCGACACTTAGGAG30.2762430939226519No Hit
TATATACTCAAATATATTGCTGAAAAACGATCGAATCAGTTTTTTCTTTT30.2762430939226519No Hit
AAACTGACTTATCAAGCTAATTACATACTTTGATTTGATTTGATTGTAAG30.2762430939226519No Hit
GTCGCTTCCTACGACATGCTTGAGCCACAGTGATCCTATTCTTGCCGTTT30.2762430939226519No Hit
AGAAACAGGAGCCAGAACTCTAATCCTGTAGCTCATAATTTAATTAAATA30.2762430939226519No Hit
AGACGGGCAGTTCCTTCCAACTAATCAAAGTCGATTGTTTGTGTGTTTTG30.2762430939226519No Hit
CTAGAATGCGAAGAAGGATTTGTGGGCGGAGCCACGAGCGTTCATGCTGT30.2762430939226519No Hit
CACACGGGAAAGATTTCCGCCTTTTTTACCATCTAGGGCCATGTCCGACT30.2762430939226519No Hit
CGAAGAAGAGCAAGAAGAGGAACAATGATAGAGCAACAACTTCATCTAGA30.2762430939226519No Hit
TTTTGAACAAAATAGTTTCTACTCTTTGATAAATCAGGTTCATGCATTTT30.2762430939226519No Hit
TGGATTTTGAAGGAATTCCATCAAAGGAGTTGACAGATGCTTCCCTGTCC30.2762430939226519No Hit
GCTAACTGAACGGAACAGCTAACCCGCCACTACTGAACGGAACAGCTCAC20.1841620626151013No Hit
TGTTTGTAAAAAACAAAAAGACTTGTAGCCCAAATATCAATTTCATTATA20.1841620626151013No Hit
GGCGAGAGAAACACCGACGACTGAGAGAGAAGAAAGACGCTTTCAGAGCA20.1841620626151013No Hit
TATAAGTCCTTTGAATTCCACTTTGCATTCTAACATCCAAACAAAATAGA20.1841620626151013No Hit
CATACATGTACTCTAAAGATATGGCGAATATGAGAACTTGAGTAAATCAT20.1841620626151013No Hit
CCCAATCATAAAATGATTGGCAATGTTGATCCTCTTTTACTTAAGTATTG20.1841620626151013No Hit
GCATTGTATTGGGTTGTCTCATCATTGTGTTTGATTGTCTCATCATTGTG20.1841620626151013No Hit
CTGAGGACAAGAAAACGAAAGCTGCCATGAAGGCATCTGGAAAGAAGAAA20.1841620626151013No Hit
AACATGGTTCCAGAAAATTGTAATTTTGGGAAAAGTGGCATTGTCATATG20.1841620626151013No Hit
GTTGTCTTGACCACTCACAGAGTTCTTTGTACGAAGTTACAGTCGAATGT20.1841620626151013No Hit
AAAAAATCAAATGAAGAGGGATTTAATTCGAAATCTACAGGGATGGACCC20.1841620626151013No Hit
CATTGCTTTCTTATAACCGCATACGGGTATCTTTTTTTCTCTTTCATGTT20.1841620626151013No Hit
CTTCAATTAGCATTTGTGTGGAGCTTGTGATCCAGTGGTAGAGCATCAAT20.1841620626151013No Hit
TTTTAATACAAGTTGTGCTGAGTTAGTTTTATCAATTTTATGCAGTTGTA20.1841620626151013No Hit
GTGGGGCGTTAGTTGGCTTGAGGGTTTTAAATCAAGTTACTTTTAGGGTG20.1841620626151013No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCC20.1841620626151013No Hit
TGTAAGTGCACCTGTACCCAAGAAACTGTTGACCATGGCGGGTATTGAAG20.1841620626151013No Hit
ACGTATTTCATAGTTTTTATATACTTGCTTAAAGTATGCGCTTTTATAAA20.1841620626151013No Hit
AATTGACCAAATGCTCAGAAAAATTAGTTCCATTTTCAGTTATCAGCAAA20.1841620626151013No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1841620626151013No Hit
CAGCACTTTAACTCAGCAAATAAGATCAATAAATACATTTGCAAGATTAT20.1841620626151013No Hit
GTGAAAAATACACGAGACACGTAAAAATGTTGTTCGGTGTTACAGTGGCC20.1841620626151013No Hit
AGTCAGTCATCACGACCCAGTCATCCACATGGACAAGAACAAGGTAGAGA20.1841620626151013No Hit
CAACATTATTGGAATTTAACCATGCTCATTTAATTGTGTTGTCACTAAAA20.1841620626151013No Hit
CGGGCGGGATTCATTACTTTAATTGTATATGTTAACACATATAGATGTTG20.1841620626151013No Hit
AAACGTTTTGTTTGACTATAAATTATTGAAGAAGTGATATAACTCTATTT20.1841620626151013No Hit
AATGAAATGTTTATTGTAATGATGACAGACAGAAAGAACTATTTTTAAAT20.1841620626151013No Hit
CGTAAAACCCTATTTATTCATTCCATATCTATGGTAGAACCACCTATAAC20.1841620626151013No Hit
GCAGGCCCTATTTCCGCAACTACTATCCAAGAGGAGGGTTCCGGGGAGGT20.1841620626151013No Hit
GGAACTTAAAGAACTTGCCGCCCTTGCTTACCGTGACACTACCGCTGAGA20.1841620626151013No Hit
ATACCGCACATTTGCACACTAACAAGAATAAACGTAATATTAAATCTGAC20.1841620626151013No Hit
CCGGTTTTGACAAGTTCAAGGAAGTTTACCTTGCATGGCGTCTTGGCAAC20.1841620626151013No Hit
GAACTTGTAAAAAAATTAGGTACTCCTTTAATAAATATAAACACGAAAGA20.1841620626151013No Hit
CTAACATGTATATATACACATGACTGTGCAGTGGTGTTGTGTGTTCTGAA20.1841620626151013No Hit
TGGGATACTGTTCTTCAACAACAGCACATGCAATCCATTAACAAGCATTT20.1841620626151013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)