FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-6_S47_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-6_S47_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086
Sequences flagged as poor quality0
Sequence length84-151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC12311.32596685082873No Hit
CAATGTTCTAGGCTACATTCAACAAGCTTTCTAGCATGCTAAATGGACAA80.7366482504604052No Hit
GGAAGTGGAACCATCTTCCTCTCGAAAGTGTTTGGTGGATTCCTCAGTTG70.6445672191528545No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.6445672191528545No Hit
CCATAATCAATACAGTTTTACCAACACCAGCTCCACCAAACAAACCAATT70.6445672191528545No Hit
AAATGTTCCAAATTTTCTTTCGAAAACAGATCTCATAAAAACCTACTATT70.6445672191528545No Hit
GGACAACATCACCTCTGTACAACATACAGCAGGCCATGTACTTGCCGTGA60.5524861878453038No Hit
CCTGTATAATGCAGGCTCGTGTTATTGGAGTTAGTTTACGTATATACATT60.5524861878453038No Hit
GAATCCGCATATGCTTCTTCATGTTCATCTTGGATATGGACTTGAAACAC60.5524861878453038No Hit
GGCATGTGGACCATGCAATTCATGGTTTTGATTCACTCTTCGGTACCAAA60.5524861878453038No Hit
TTTTCTTCTTTGGTGTTTGTTTGTTGACGACAGGAGTGGCAGCTGCCTGG60.5524861878453038No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.5524861878453038No Hit
AAAAAATTAAATTTAATTCTAAATACAAATGTACCCATTACAATAATTTG60.5524861878453038No Hit
GGTTTATCGCCATTTTCAACACATTTCAGGGTAATATAATGGCATATGCA60.5524861878453038No Hit
TTGGTGACAACCCAGTAAAAATGCAGACGCTACAGTGCACACAACAAAAA60.5524861878453038No Hit
GAAGAACCAATTTTTTTTCTTCATCATACCGTGTTGACTACGTTGTTGAT50.46040515653775327No Hit
TGAAAATCTAGAAATTAATTTTTATGGCCTAAAACATAATGTTTCTGTTA50.46040515653775327No Hit
TTTTTTACAATGCAATGATTTATTTTGTTTACCACACTCAATCAAAATAG50.46040515653775327No Hit
GCAAAGTCAATGTGAGACAAGAAATTTTAATGTGAAACAAAGTCAATGTG50.46040515653775327No Hit
CGGCAGTATTTAACAACGGCACCCCAAAAGCATTAATGGGCAAAACCCCT50.46040515653775327No Hit
TCAAGCTCCCTCAGTTGCTGTTGCAGTCTCTTGTTCTTTTCTATTTCAAT50.46040515653775327No Hit
AAAACATGCAACCCATGAAATCCTGTCATCACAAAAAATACAGAGCCATA50.46040515653775327No Hit
GCCAGATATCCCGGTATGATATGTACTTGTATATGTTCAGATTTAAGTGG50.46040515653775327No Hit
TTTTTTTTAAGTCGTAATAAAATCTTTATTATTTTTTTCAAATTCTGGTC40.3683241252302026No Hit
ATATTACATGTATTAAATGTGCTCAATACTAACTACATTGCTCGTAAAAG40.3683241252302026No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3683241252302026No Hit
GGCCTTGGTCGAGTAGTCTCTGGCCTGGTCTGGCGTTTCAGTGTGGCTGG40.3683241252302026No Hit
TACATATAGATTCCCCGACGGCAAATGATTGCACTAGGCCCATCTGGTAA40.3683241252302026No Hit
AAGGTTTTTTGCTTTCCTCCACATTTATGGAAAATCTCGTTAATGGTACT40.3683241252302026No Hit
CCAGTAATAGTCCATAAAACACCATTAACCGTTGTTGAACCATTGTATTC40.3683241252302026No Hit
ACTGCCTTAGACCACATAGAGGATCCATATTGTTTTCAACAAAAAGTTTT40.3683241252302026No Hit
CGCCTATATACTCTTCCAACTGAAGAACTGAAAAATACCCACCAAGAACT40.3683241252302026No Hit
CTCCTGTTTACTGATCCACATCTGCTGGAAGGTGGACAGAGAAGCCAAGA40.3683241252302026No Hit
CACTTTTCTCCTCACTTTCCAATTTATCATAATTTAAGGTGCCTTATTAA40.3683241252302026No Hit
GTGGGGTTCATCCAACAGCTCATTGAATTGTTCACCAGCGGTTTATTCGA40.3683241252302026No Hit
CTCACATGCCAGAAATCACAGTTTACAGATACGTACTCCAGCAATTTTAA40.3683241252302026No Hit
CACAAAACTCAATTAACAACAATATAAATAACATGTTGAATGGATGGAAT40.3683241252302026No Hit
AAAAAACTTGGATGATAATCTAGCAATTAATTTAAAACGGTCTAATGAAC40.3683241252302026No Hit
AAATCTGGTTGATGCGTCTTAATGGGCAGGCATTCAAATGGAATGGTCCT40.3683241252302026No Hit
GCCACTTCCTCAGCTTCCTCAACTTGGCGTTTATATGTCTTAATCTTGCT40.3683241252302026No Hit
GTTCGATGACAACTTTGGTGTCCATGGAGGAATGCTGTTAATCTCCGCAT40.3683241252302026No Hit
CAAATTTCGAATTTCTTGTTTTAAACAAATAACATAACTATGTAACCATT40.3683241252302026No Hit
CTTCCAGTTCCTTTGCAGTCTTATCCATCTTGGCTCTAGAGAAATCATCG40.3683241252302026No Hit
TGCTTTGGTCTCCTCCGCAGCACCTGCGGCTGGGGCTCCACCAGCTGGGG40.3683241252302026No Hit
ACATCATTAAGAATTTCAATGCATCCCTTCGTGATCTTAGTTGTAATCTG30.2762430939226519No Hit
TAGTTAAAAACACCCCTCACTGCTTGCAGAATTATTGAACATTGTAGCAT30.2762430939226519No Hit
TTTCGATCTTCTGTTCCAGTGGTATGGGTTCTGGGAAAGATTTTGGTACA30.2762430939226519No Hit
TGACACGTCGTCATGTTATGTTGATCATTTGTAACAAGTATTCATAAAAT30.2762430939226519No Hit
CAAGATATGTAGCACATTGGTTATTGTTCCGAGGGCATGTTTGAAATGTT30.2762430939226519No Hit
CCAAAACCTCTTGATCGTTTAGTGGTTGGGTCCCTCATAACTACAACATC30.2762430939226519No Hit
AAAATGCTGTTTTTGATTGGATCCAACCTAGTCTACCACTTGTTTTATGA30.2762430939226519No Hit
GTAGAGTTAAGTTAGTTTGATATTCATCTATTCATCATGAATCTTAAGCT30.2762430939226519No Hit
GATGAGGTCAGCACATACCTTTTCCAAGCTCTTGACATTCCTGCTGGTCA30.2762430939226519No Hit
CCAATATGAATAACACATAATCTTAAAGCTATTTGACGACAGATAATTTA30.2762430939226519No Hit
AGTAATTGCAGTTGGATCAAATTTAGGAGGCATTTTGCTTGTTGTTTAAA30.2762430939226519No Hit
AAATTCATAATCTTTAATATGTTTTGTTTCAAACATCTTGCTATATTCAA30.2762430939226519No Hit
CGATCTTGGAGCGCTTTCTTCTGCTGCAGCAGGTCGATCAATCTTCGAGC30.2762430939226519No Hit
GTTGTTAGTTCACTGTAACTATATTTCTAAATAACTACAGTCATAAAATA30.2762430939226519No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2762430939226519No Hit
CAATACACAAAAAATCCGTGTACTGGTAGCCAGAAAAAAGCGGCACGAAA30.2762430939226519No Hit
AGCACTTACAAACATTTGTTTGTATAAAGCAGGTGATGTAATAGTTTCTT30.2762430939226519No Hit
CTATCACTGTCAACCTCTGTAGAGAGGTAGGAATGGGTGTCATGTTCATT30.2762430939226519No Hit
TTAAATTGCAGTGCAGTTTTCTCTCTGCTTTATTGTTTGTAAATATGATG30.2762430939226519No Hit
TTTTTTTCAATTAAAAACACTTTATTCATTTTTTCGAAAATTTTGGGCCA30.2762430939226519No Hit
NCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2762430939226519No Hit
CGAATATGCTCGGTGAATTCCGTAAACTGTTCAATATCAGTAATGTCAGT30.2762430939226519No Hit
AAACATGGAATACAGAAGTCAACATGAAATAATTATATTATTGTTTTAAA30.2762430939226519No Hit
GCGAGATATTGTGGTTATATAACTGCTGTGCAAGTTTGATTAAGATTCAA30.2762430939226519No Hit
GTTTGACCCTGTCTGATGAAGAAAAATAAGATGAACTACCGCTAAACCCC20.1841620626151013No Hit
GGTAAATAAAAAAAAAGTGAAATAGCACTTTTAAAATGGTAACGCATTAT20.1841620626151013No Hit
GTCAGAAATTGATTTACAATTTAATTGACAAACTAGAGATTATAAATAAT20.1841620626151013No Hit
AGAAGCGACGATAGAATGGTCGTCTTCGATCAGGAGCTCCCATTGGTCCT20.1841620626151013No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC20.1841620626151013No Hit
GGGAATAGCATTTTATCTGTAACAATTGTTGTTTTATTTTGTTTACTGAT20.1841620626151013No Hit
CTAAGATCCGTTAACTGGCGAGTAACCTTCTTGTTCTCCTCTTTGATGTG20.1841620626151013No Hit
TTTTCGATCAATATCTTTATTTGCTTTTTTTTTTAAATTTTGCACTTAAC20.1841620626151013No Hit
ATTGTATGTCACTGTAGAATAGCACCAAGAAAATCGTTGCTCATCTGTCA20.1841620626151013No Hit
GACTGCTGCAAAATTATCAAACATATTCCTTCAATGATTATCACTGTAAT20.1841620626151013No Hit
TTTTCAACTGGCATCAGGATTTTAATAACACTCATAATTCAATAGTGCAA20.1841620626151013No Hit
ATACTAATTTCTTTATTAAAAAAAAATTGAAATAAGAGTCAACAATTCCT20.1841620626151013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1841620626151013No Hit
TTTTATTTTTTTTTATTTGTAAACATCCAACCAATAGTTTATTCTGCTTC20.1841620626151013No Hit
TAAAAAGTGTGTTTTTGAAAACTCTCTTTGTTCATTTTGGCCCAAAAGAT20.1841620626151013No Hit
GCCATATCCGCTTTTCACGTTAAACCATTTTACACTGCCGCTGACTTTTG20.1841620626151013No Hit
TTGAAGGTTAAAAATCAAAATTGGCAATATCCAGGAAGAATGCACTGACC20.1841620626151013No Hit
TTATTACCGGCCATAGCCAACATCCATGTTGGTATTTTTATAAAGTTAGG20.1841620626151013No Hit
GACATTGTTGTGCAACCATTGATTTTTTATGGAGAATTATTAGGCCATTT20.1841620626151013No Hit
CTTCTATACTGGTAGTAGTAAGATCTCCATAAGCATTCAGATCCAAAATA20.1841620626151013No Hit
GGATATTTCTTTTCAAGTTTACCACTCAGATTGAAATATTTTTTTATTGG20.1841620626151013No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCG20.1841620626151013No Hit
GTTGTATAAAGTTTTTAGGAATTTTTTCTCTCACGAGTCTATAATGCTCG20.1841620626151013No Hit
CGGAGATAGGTCGCCGTTTTTTAGAATTCCCGATATCCGGTAGTATTGTT20.1841620626151013No Hit
TTTAAGTTCTTAATGATGGCAAATTTATTGGTCACAATGACGGATGAGCA20.1841620626151013No Hit
GATCTTTATCGTCGCACTCTGCATCAATCAGATTGGCGGTGACACGCATA20.1841620626151013No Hit
TAAATCAATATTTAATCACCGTATACCCAACAAAAAGTAAACAGAATATC20.1841620626151013No Hit
GTGAAAGTTAATAATGGAATCAATCCATATTCCTTCCCCCTATATAAATT20.1841620626151013No Hit
GGGGATTCAAACTCACAATCCCTGGACTGATAGTCAGATGTGTAACCGAG20.1841620626151013No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC20.1841620626151013No Hit
ATTTCTCTCTATTTTATCATAATCATATATAAAATGCTAATTTGTTTAAA20.1841620626151013No Hit
TGAGGAGAGGACCAAGTACAATGCCATGACAACCAAACATAATGAGGCAA20.1841620626151013No Hit
CAAACCCGTGTTTCCTAAAGTATATTGCAAAAAAAATATTTACATTAAAA20.1841620626151013No Hit
CCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1841620626151013No Hit
GTCACAGTGCGGGAGTTTTTATCTCCGCTATAGGCTGAGATAGAAATATC20.1841620626151013No Hit
GCTAATTGTATGTTACAATAGGAAAATAAATTCGTGTAACATTCCTATAT20.1841620626151013No Hit
CTCCTTTTCCTTACGACGAAGATCGGAAACAAAGTCACTGAATATAGCTT20.1841620626151013No Hit
TGAAGCCGTATTGCTTGCCTGTACATCTATATCTGCAATTTCTCAAACTT20.1841620626151013No Hit
GGTGACTTGGTGAAAACAGTCTCCTTCCACAGGTCTGGGGTCAGATAGGC20.1841620626151013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)