FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-5_S39_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-5_S39_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203
Sequences flagged as poor quality0
Sequence length82-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAATCTCATTAACCATTGCATGAATTGCCTTGAAAATTTACACAAAAC151.2468827930174564No Hit
GTTTTGTTGTCATGGAAACATATAATTGGTTGTCGTGGAAACATGCAGCT141.1637572734829593No Hit
AAATTATTACCATGTGTCATTGTACGGTTAAACATATTGAATGCAATCTA131.0806317539484622No Hit
TTCAGCTTGAGTTTTCTTGCATATCGGATAAGGATATCTACATTTTTACC110.914380714879468No Hit
GAACAAACTATTGGAGAAACAGAGTAATGAAAGTAGCAAAGGGATTCCAA110.914380714879468No Hit
CCGTAGGAAATGAAAATGTTAAGACGTCTGATAGAAATTCACACCAAGGA100.8312551953449709No Hit
GCTTAACCTACTAGTTGGATAATTTGGCATTTTTATTATTGTTATTTTTA100.8312551953449709No Hit
CTTGAATACAGGTCTCTGTATGTATGTAATGGGAATATAGGCCTAAGTGT100.8312551953449709No Hit
TGCAGACGTATGCCACAAAAAATGAACAGTTTTGGACGCATTTCAACATT100.8312551953449709No Hit
GTTTAATGGGAATTTGATGCGGGGTTTGAGGTGTCGTAATAGGAGTAGTA100.8312551953449709No Hit
GTTTGTTTTGCCTAGAGGGGAGAATATTCGTGTTTTAGTGGGGTCGGAGG100.8312551953449709No Hit
GTGAATGTAAGGACGGCAAGACGGGATTCTACGAATGCAATACGCTATGC100.8312551953449709No Hit
GTTAATGCAGCGAGGAATACCAATATGCCAATAATAATTCATTGACAATG90.7481296758104738No Hit
TGTTTCTTTCAATATATAGTGTAACTTATTGTGAAATAATTACTTAGATT90.7481296758104738No Hit
GGCCAGCGAAAAGTGTATCATAGTTGACTGGAATTCTGAGTGTCTCTGAT90.7481296758104738No Hit
GCTAATTTGGTATAGTCTTATATTAAAGCCTAATTTTCAAGTTGGATAAT90.7481296758104738No Hit
CAACCACTCATTGAATGTGAATGAAACTTCACAAGTGTGTTCACCTTCAT90.7481296758104738No Hit
TTTGATTCATATAAAAAAAGAATGAATTATTCAGATTTTATATAAACCTA90.7481296758104738No Hit
CTCGCTGAAAGAATGAACACAGGTCTGAAGAAACTTGTTGAAGCATCAGC80.6650041562759768No Hit
AAAAATTCAGACACTTAATTATAACAATGTCTGCTTATCTGGATTTAAAC80.6650041562759768No Hit
GGAGTTTATCAGGTGAAAATGCTATAAATGTCATAAAAGTTGTCTAAAAT80.6650041562759768No Hit
TGGTTTCGCTAATTTTAAATCACACCTTTTTGAAACTTTATTTATGCTTT80.6650041562759768No Hit
CTTTACTTCATATAGGCGCTTTTGGACACCTCTAATATAGACACCTTTGG80.6650041562759768No Hit
GGTCAATAAATCTTGGATAACAAGGAAGCACTTGTTTTATTTTGTATGTC80.6650041562759768No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.6650041562759768No Hit
AATCTGCTTACATTCATGAATTGTAAGAAAGCTTGTAATCTACTTCCATT80.6650041562759768No Hit
AACGAATAGAAAAATTGAGGAAGAATGCCTTCAGTAGTATGTGTGTATTG80.6650041562759768No Hit
GGCAGCTGATCAAGCAGTGTTACTTCATAATGGAAAATCGTGAAGGAATC80.6650041562759768No Hit
GTTCATGAACACTTATGATACTCAACTGACTGCGTCGGCGCTGTTCCTCC80.6650041562759768No Hit
CATAAAGCGGTTTTATTATTATTATTATTATTATTATTCATACATGCCAA80.6650041562759768No Hit
GAAAAGTTTTTGGCATAGACTGCAATAATAACATAAATTTTGTAGTGTGT70.5818786367414797No Hit
CGTACAACGACAACAAACTAGCAATAAAAAACAAATTATTTATGCATTAA70.5818786367414797No Hit
GCAAGGTCGACCATCAGCAAAAAAGAAAAAGGTCAAGAAACCACCAAAGG70.5818786367414797No Hit
CTTGCCTCCAAGATGCAGTTAGTAAGTGAAGTAATCATGCTTAAATGTAC70.5818786367414797No Hit
TGACGCCCCTACTGTTGAGGCTGCAGAGCAAGATTTCAGACTTTTGTTTC70.5818786367414797No Hit
TGGACTACTCCTTCCGGTGGTAGTGGTGCCTTGGCAACGTCAGCTCCAGA70.5818786367414797No Hit
TTCAAGGAAGTTTACCTTGCATGGCGTCTTGGCAACTAATGATAATGCAA70.5818786367414797No Hit
CGGACCTGATTTCTCCAATGTTACAAGCCGGTATCGACTATTGTTTCCAC70.5818786367414797No Hit
TGTTTTGTGGCTCTAATTGATGACTGATTGATTGAATATCTAGCGTCATT70.5818786367414797No Hit
GTCACTTACAAAATCATTTGCTATTCGCGAATATTGTTCTGGATTAGTTT70.5818786367414797No Hit
GTATGTCCATGAAGGAGGTTGACGAACAGATGTTGAACGTGCAGAACAAG60.4987531172069825No Hit
GATAATTTGATTTATAAATCTTTGTAATATTTATCTGTCTATTAACCCAG60.4987531172069825No Hit
GTTTTCTTTATCCCCAACAAAATGCAGATCTCGTCCAAAAATTGAATGCA60.4987531172069825No Hit
CGATAACTTAAAAAACATGATATTCGAAGTGTTAACTGATGCAGCCCATA60.4987531172069825No Hit
GTCAGATCTAGTGTATGTGTTTTATATGCAGGCATTATAGTGTCTCTGTG60.4987531172069825No Hit
GAGACAAGTATAACGCTCTGTTTGGGGCGATGCTAAATCTGGCTTCTCGC60.4987531172069825No Hit
CTCAGCTGCAATGACCAGTTTTTGTTTAGTTGTAAATAAGTAATACTATC60.4987531172069825No Hit
CTGTATCACCACTCCATTGTTGTGTAGGATGATATAACCATTAATGTATC60.4987531172069825No Hit
CTTGAATTCATTATTTTAGCTTAGCTTCATTATATTAGCTTACTAATAAA60.4987531172069825No Hit
AGGTCGGGAAAGTCTATCACATTCGTCACACAGTATGATGTTGAACTTTA60.4987531172069825No Hit
TTTGAATCACTCTCGAATCTCCTCCCTGGGGAGAACCAGTAATGAGTGCT60.4987531172069825No Hit
TGTGCAGCAGGAGCTTCAGCGAGTGCAAATGGAGGAGCAAACAGCGCGGA60.4987531172069825No Hit
TGAAAATGGCAGTTAACCCATTCTGACGCTATGCAAGACTATAGTTTTAC60.4987531172069825No Hit
CCATGATAAATCTGCTGTGGATAGCGGTCAACTTTATGTTCCAGCTTAAT60.4987531172069825No Hit
GGACCATCACGTGCGGACAGCTTTGAATATGTGATGCACGGAAAAGTGTA60.4987531172069825No Hit
GTTCCAAGAAGACTCGGTCCAAAGAGAGCCAGCAAAATCCGGCGTTTGTT60.4987531172069825No Hit
CAGTCCAGTTGTGCGTGTTGCTGTTGAATGTAAAAACCCATCTGAGCTGC60.4987531172069825No Hit
GTTATAAGCTTTTCTTGCTATTTATAGCCTTTATTATTAAGCGTAAAGAT60.4987531172069825No Hit
CTCATGGATACAAACCCAGATGGGATCAAAATGAACATCTGTAACGAAAC60.4987531172069825No Hit
TGTATACATGTGATACAGGACTTTGCGTAACAACTGGCAGAGACATAAGA60.4987531172069825No Hit
AAAACGTGAGATGCGGGTGATTGAAAAAATGTTTTTATATTGATTTTTAT60.4987531172069825No Hit
CTCACCCAAGAACAAGTTGATTCTGATGAAGATATCAACCAAGATTAAAT60.4987531172069825No Hit
TGAAAAGGTTGCAGCTGGCAAGAAGAGCCAGAAGGCAGAATCCAAGCCAG50.41562759767248547No Hit
AAACTATACAGTTAAAATAGCCATCTGTAATGGCGAGTGTGGCAGCAAAC50.41562759767248547No Hit
CGAAGAACATGCCTCACGAGACGTGGAGAAGATGATCCTGGGAAACAAAT50.41562759767248547No Hit
CTCCATTTCAGTATGCAAGTCATAAGGTCACTAGATGGTATACATGTACT50.41562759767248547No Hit
CATGCAACTAATGTGATATGCTTCTTATTAAGATTATTGGAATTTAAACT50.41562759767248547No Hit
TCTTTATTAACCAAATACTGACAGATGTGTTAATAGTTAAATTATTTATT50.41562759767248547No Hit
GTTGGGGATTATCAGTATTGAGTCATGTTGTGTTCCGGTCCATTGACTTT50.41562759767248547No Hit
CTTCAAGAAGGTCTACAAAAGGCTGTGTACACGCAGTATGATAGAAAAAT50.41562759767248547No Hit
CGACGAGTAAAGTTCCAATGATATGGTCGGTAGTAATTAAAGTAGTTGGG50.41562759767248547No Hit
GACCAAACAGCATCAAATTGAAGTAATGGGAATGCAAAGTAGGATAAGCC50.41562759767248547No Hit
GGTAATGGTCAGAATCGAACGTTGCTCGATCACCATGTTCCAGGGGTTAA50.41562759767248547No Hit
CCGCCATCCAGGAACTGTTCAAGCGTGTCTCCGAGCAGTTCACCGCTATG50.41562759767248547No Hit
TGAACATAATTACCAATAAAAACGTTTATAAAATTTCAACTTTATAACAG50.41562759767248547No Hit
CCCAGGGTGACAGCGATACTGCAAATGTGATCATGGGCATCAACGCACAG40.3325020781379884No Hit
TTTAAAAGTCATACAGCTACTGGTGCCAGAGTTTTATAGAATTTGGTGCA40.3325020781379884No Hit
TGGAATTTGTAGTGCGGGAAGGCCCCATGTTTGAAGCAATGATAATGAAT40.3325020781379884No Hit
GTTGTTTCTAACATTGTGGTGAAAGGTATATTTTATTATTTACCAATCTG40.3325020781379884No Hit
GACTTCTACTATCATATGTACGGCAACGGCATCGGCTCATTGAAACTGAA40.3325020781379884No Hit
AAAACAGTCTCAACCATATGAACATAATAATCAAAGGACTCAAGGTCCTG40.3325020781379884No Hit
TTCTTCAAATCTCTTCATCACGAACACCCGACGGTGAATCAAGTGGGCGT40.3325020781379884No Hit
TGCCCTTGTCTTTTATGCTGGTATATATAATCTACAATTTACATTTATAT40.3325020781379884No Hit
CTAGAGGGGAGAATATTCGTGTTTTAGTGGGGTCGGAGGATGTGATGCAT40.3325020781379884No Hit
ACAATGTTTGTACATGTATTCATTCATGTAAGGCTGCACTTGTAAGCGCA40.3325020781379884No Hit
AACGGCAGCGATCTGGCATAGTTTCATTTAAAATGAATCTTTGATGTAGG40.3325020781379884No Hit
GTACTAAAGTCATGGTAGTCTCTATTTACAAATAACCATTGGTTAAATAC40.3325020781379884No Hit
GTTTCGTTTGATATCCGCCAGGTTTAAATATACTGTAGGTTGATCATACT40.3325020781379884No Hit
AAATGCTTTACTTTTTATATGGACATGATTTTATTACACTCTTTCATATT30.24937655860349126No Hit
CAGAACCAAGACACCAGGACCTGGTGCTCAGTCTGCCCTCAGAGCCTTGG30.24937655860349126No Hit
TGACAACACAGGTCAGGTCATTAAGTAATATAACAACATTGTGACTGTTA30.24937655860349126No Hit
TGAACAGTTCATGCGTGGAGTGTTACTGCGATCAAACCGGATATGGATGT30.24937655860349126No Hit
TTTAGGTCAGTAGTTAGGTTGTTCTATTACCTTAATATGTTTTTTAGGCT30.24937655860349126No Hit
CGTGCTTCTCATCAGCGTCTTTGGGTTTATTGCCGCATTTTGTAAACTCA30.24937655860349126No Hit
GTTTTCTTTATCCCCAACAAAATGCAGATCTCGTCCAAAATTGAATGCAC30.24937655860349126No Hit
TTTCAAACCATTTTTTTGTTTTCATACGGTAACTTTTTCAACCAGGAAAT30.24937655860349126No Hit
AACATATTTAAATAAAAGAGAAAACCTATACATAACTAGGCTGCATTCAT30.24937655860349126No Hit
AGCAAGGGCAGATACTAGATGTCAGAATTTGTTTGTATGAAATTCTTAAG30.24937655860349126No Hit
TTTACTCATCTCCTATATCTGTATCTGCTGTATGAATCTCTATGTTACAC30.24937655860349126No Hit
GTTTTTTTATGTTTAAATATATGTTTATGAGAGACGTCATTCAGTGAACG30.24937655860349126No Hit
CTTTTATGGCATCAAAGACCTTATGGAAGAAGAGAAATGTTAAATTGGAT30.24937655860349126No Hit
CAATTTGTTTTAAGCAGCCACCTGTATTAAGGGGCCATCTTTGCTCTTTC30.24937655860349126No Hit
GCATATGCTCGCATGCTACTCATACTGGCGCGTCTTGTGCTAATGGCGGT30.24937655860349126No Hit
GTAGTTTTCCTCGTGGCCTATATATTCGGATTGGTTTTGTGTTGCTGGAG30.24937655860349126No Hit
CAGATGCCACCGTCAAACCTATGGACTATTCAGGATTTTCTGGTCCCCGT20.1662510390689942No Hit
TATACAAATTAGTACTTCAACAATTTTAAACAAAAATTTCTTGGGATGGT20.1662510390689942No Hit
GCTTTGAAAGTAGTACCAGCAATTGCACCTTTCGCTTTGGCGACTGAGAT20.1662510390689942No Hit
CTTTAAACATTATGTTTTTTGTTTTATTTATTAATTGTTGGGCCCAGTTT20.1662510390689942No Hit
CGCGGAGGCAGGACAGCAGCCCAGAACATAATCCCATCTTCCACTGGTGC20.1662510390689942No Hit
GCTTTTTGACAAAACTTTAAGCAAGGCCGATGCAAAGTGATTTACTTCAA20.1662510390689942No Hit
GGAACGACTACGACCACTACCACCGTATCACCAGAAGCATCAACTTCAAT20.1662510390689942No Hit
CTTGTACCGGTAAGTAATAAAATATGTTTTGAACATGGCATCAAATTTTA20.1662510390689942No Hit
GTTATAAGCTTTTCTTGCTATTTATAGCCTTTATTATTAAGTGTAAAGAT20.1662510390689942No Hit
CCGACTCCGTCAGATCAACAATGGAGTATTCGTGCACCTCAATAAGGCCA20.1662510390689942No Hit
CTTCCTGCGCTAATGGAATATGGATGTCATGCATAGCATGTGATGCGTTA20.1662510390689942No Hit
GGCAGATGTGAGTTTGTGTTTCTACCTGGAAAGTAATTCATCTTCATCTT20.1662510390689942No Hit
TATGTGCCGGTGTTTGCAATTTACTCAAATTACTGTTGGAAATTTGCAAG20.1662510390689942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)