FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-5_S39_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-5_S39_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203
Sequences flagged as poor quality0
Sequence length82-151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC262.1612635078969245No Hit
AAAATCATATTTTAGGTTAAGAAGATAACAGTGGTTTGAAAATGAAATGA131.0806317539484622No Hit
TTTTTTTATAAATACAATTGCTTTTTATTTTACTATAACCTTAAGATAAA110.914380714879468No Hit
GCAAATCTCAGAACACTGTCCATACACTACACCAACATGATTTCTCTTTA110.914380714879468No Hit
GCACATCGTAAAGCCGGACGATGGCACCAAGGTTCCTGGCTGTACCAGCA100.8312551953449709No Hit
CTTTTTCTTGCATACCAAACACATTGAATATTAATAACTTGATCTATCAT100.8312551953449709No Hit
GCCCTATAAACATAATCGTACCTTCAATATCACTGATGCCCAATTACCTT100.8312551953449709No Hit
GCAAGAGTTATCTCACTTAGTTATTTTAGTGGGTCTGGTAACTAGGAAGT100.8312551953449709No Hit
ATTAATCCAACCTTTCAAATAAGTAAAACTGACAACATTAAACAATTATA90.7481296758104738No Hit
GCAACCCAGAGTTATGGTTCTTGTTGTGTTTGTTCCATTGATGATGGTGA90.7481296758104738No Hit
GGCCATAAGAAATTTCTGGTCCTTGGCATTCCTAGCTGCGATGATTGGTT90.7481296758104738No Hit
TAGCAATATTACATATTATACTACAACGTTTTTCTTTGTTTTATTTTCTG90.7481296758104738No Hit
ATAATTTAATTATTTTCAAATAAATATAAAAAGATAAGCTTTAATGCACA90.7481296758104738No Hit
CCGCCGTCCAGAACATGAAATATTGGAATGTTATTTTTTTGCTTTGATTT80.6650041562759768No Hit
TGACAAAGTGTCCATCTTTCTTCGTTTCAAAATCAACTATATATAAACCA80.6650041562759768No Hit
GTTAAGCATAGTTAAAGTGATACAGAAGGACAAAACAAAAGATTTTATTA80.6650041562759768No Hit
GACAGTTTGGTATTGGACAACACTTTCCAGTTGGGGCAGGGTCCATGTGA80.6650041562759768No Hit
TTATGCCCTTAAATGTAAACTAGAACAGTTAAGATGTAATTTTCTGTAAT80.6650041562759768No Hit
GCTGGGTGCTGCCGGAGGGGGTCCAGATGGTTGGCGTGCTATTGTCTTTT70.5818786367414797No Hit
TTTTAAGGATCTAGTGATTTTTATTTCAACATTTTCAGAAAATATACCGA70.5818786367414797No Hit
CATCCGAAGATCGAACCGACGACCCTCTGACCATGGGTTTGGCGCTCTAC70.5818786367414797No Hit
ACAACAGTTACTACTTACGGTTATTCACAACAAAACTTTTATCTGATTGA70.5818786367414797No Hit
AATTTTCCAGTCATCACTTAACTCAAATGCCAGCTCATGTCCTTTCCATA70.5818786367414797No Hit
CGAAAAATTTCATTAAGACCCGATCACTAACCCAAGTGACAGGACACCGA70.5818786367414797No Hit
GTGGTGTCCATGTTGGGGTGATGTCATTTTGGCTGTATTTGACTAGTGTA70.5818786367414797No Hit
GACAGGTACAAACACGGATCAATAAATATACTCGTATTTGACCAATGATA70.5818786367414797No Hit
CGCATTTGCGAATAAACGATATATATGTTTTTAATTTGGGAGTCAAAATT60.4987531172069825No Hit
TTTTGATTAACAAATTTTTATTTCCATCCAGGGAAACTATTTATGTGTAA60.4987531172069825No Hit
CACTTTCTCCGCAGCTTGAGCCGAGGTGGTCACATCAGCCAGCACAATAT60.4987531172069825No Hit
AAAAACACGAAAGGTAACTAAGACCACAACTTTGTGTTTTACAAAAACTT60.4987531172069825No Hit
CTAAATCCTGATATATAAGCTATGACTGAAACCATTTTTACAGAAATGAT60.4987531172069825No Hit
AAATATACCACAGTAATCAGCCATGGTAACAGCTGATTGGTCACAATTAT60.4987531172069825No Hit
CAAAAGTATAATCTTGTTAAAAATTTGTCTGATGAGGTTTCATATCGATG60.4987531172069825No Hit
CTCATACAGGTGCATATCAAGATAATAGTTTAAAACAGCTTTCAAAAGTT60.4987531172069825No Hit
CAGATGTGTCGTACACTTCTATTTGGAGAGGACCATGATTCTTCTTTTGA60.4987531172069825No Hit
GACATTTGCAACATGCGGAACAGATGGTACCTTCTTTTAATTTCTTGCTT60.4987531172069825No Hit
GCCACTTACACCAGGTGTTGCAGGTTCAACTTCTAGTTCGACCAAATGTC60.4987531172069825No Hit
CAAAATCCTGTACCAATTTATATATAGCTGCAACATGATCTACGTTTAAA60.4987531172069825No Hit
TTTTTTTCCATTCTGGATTTTATTGGCAAAAGTCGCATAATCTAGTATGT50.41562759767248547No Hit
GAAAAAGTCAGAATGACGATGTGAAGGTTGTCAATCAGTATTTAAGTGCT50.41562759767248547No Hit
AATTCAAAGCCTTCTATATTAAAGGTGTCCAAAGGTGTCTATATTAAAGG50.41562759767248547No Hit
TACAAATACCTCAAAATCATCTCCCATAGATACGAACACATTGATTTCAA50.41562759767248547No Hit
TTGTAGACCGTTTAGGCTTCTTTGCTGCACCATCTTCGTCTTCCTCTTCA50.41562759767248547No Hit
TCGCACTCCTGATGATTCCTCTGTGTCATCCCCAGGTTCTTCTCGGCCGC50.41562759767248547No Hit
GTTTTATTGTCAGTGTCCTCCTCCTCATTCAAATTTTCTTCAAGGTCATC50.41562759767248547No Hit
ATTTTATTTAGTAATAGTAAATGTCGCAGATGCCTGGTCGTAGATGGCGC50.41562759767248547No Hit
TGAAACCACTCCTCCATTCCTTATGATAGAATTTATGAAACGATCATTTG50.41562759767248547No Hit
GGCATATCCATTTTTATTGGTGAAGTAAGAACTCAGATTGAATTTAAGTA50.41562759767248547No Hit
GGGAAACAGGGTATATGCTTTACTTCAAAGATACAGTGTCGAGCGAGCGT50.41562759767248547No Hit
CGAGATAACAGACTTTGTAAGCCTATACACTTTATCAAGTCCATAAACAC50.41562759767248547No Hit
GCAAGACGGTGTCGTTTCCTCTGGATAACCACAGGGGTGACAAGTCGCTG50.41562759767248547No Hit
GCCGACTGTCTAAGGAGATATACATTGAAATAAAGTTAACCATAAGTTAT50.41562759767248547No Hit
CAGACTACTGTGAGTGCACCTAGCGAAAATGCAAATAATAGTCACAATAT50.41562759767248547No Hit
TTGATAGTCATTGTAAGTAGCAAAATTGACTACAATCATAGCATTCAGTA50.41562759767248547No Hit
TTTCAAACAGATTTCCAAGTGAAGTTCTCCGGCACCAGCAATGATATGCT50.41562759767248547No Hit
AACCTTATCACAGGCAGGGCATGTACCAATACAGTGGATATTTGTCAGAT50.41562759767248547No Hit
CCTATCCCATCACAATTCTTGTTGGTTCCAACACTTTTGTACACTATAAG50.41562759767248547No Hit
TGGACACGTACCCTAAGTATGGCTTTAAATGTATCGCGAATCTTGTGTGA50.41562759767248547No Hit
CTTTATATCTCTTGCTAGTGTAAAGAATGCCTCTTCAACATTTATACTGG50.41562759767248547No Hit
CATAAATAAACCAATAATGGTACACTATACAGTAATGATATTTGCAGTTA50.41562759767248547No Hit
GCAGACTGGAATTCATAGTCGAGCCCTTCATTTGCAGCTAGTTGGAGCTG50.41562759767248547No Hit
GGGAATTCAAACTCATGACCCCTTAATTGATGGTCAGATGCTCTACCGAT40.3325020781379884No Hit
TTAACATTTATTTTTAAAGCCACAGAACAAATATTTCCCTTTATTCACAG40.3325020781379884No Hit
AGTTATCTTGTTTTATTACTATTTCTATAAGTCATATACTATTCTAATGT40.3325020781379884No Hit
CCCATTTTCAGTACAATTTACGACACCCTTTTGTATTAAAGTTATCTCTT40.3325020781379884No Hit
CGTTTTCGCGTTCTACTTCGACATCCAACTCAACCTCATCACTGTTGTCC40.3325020781379884No Hit
AAACATTATATATGCCTGAATATAATACACAACACACTAGATATAACTGC40.3325020781379884No Hit
CGGGACTCGTGTTTAAGGATGTGTTCTGTTTGTTACTCGAGTATGTATCA40.3325020781379884No Hit
TAACAATTCAGGGGTCATAACTCGATGTTTATGTTGACACATATTGATAA40.3325020781379884No Hit
TTAATCGTTTTCCTTAAAGTAAAATGGCGGTACAGCACTGAAGTCAGATT40.3325020781379884No Hit
GTTATATCATCCTACACAACAATAGAGTGGTGATACATAAATTGTTATCA40.3325020781379884No Hit
CCAAGATACACTTTTATTATACAATGGATAAAAAAGGTTTCATTCAAATA40.3325020781379884No Hit
GGGTAGCATTCTTACACCAAGTAAATTATTTTCAAATGAGCCAATCAAAC40.3325020781379884No Hit
TAAAGATTTTATTTTTATTTTGATAATAAGTTAACACATATATCAAATTT40.3325020781379884No Hit
TTTTTTTACAGGAATACATGGTTTATTAAAGAATTATACACATTTCAGTC40.3325020781379884No Hit
TGAACTGAAATAAAATTAAGTTTTGTGTTCGTTAATGCATCATCGGATAC40.3325020781379884No Hit
AAAAACACAACATATTTTTGAGGGTCGTAAAACTGAACCACTTCTACAGA30.24937655860349126No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.24937655860349126No Hit
CAAAACCTAAACTCAACTAAATGCAAAGTTTAACCTGCGAGTCTACAGAC30.24937655860349126No Hit
TCAAATTCCTCTTCCTTCTCGGCAATTCGTCTGTCAATCTCTTGTCTGAT30.24937655860349126No Hit
CAGCATGTTTATTATCTCTATAGGAAAGTACATACTAAAGCTGTTTCTGC30.24937655860349126No Hit
CTCTCGCTGGTGTTCCGCCAACAATCTCTTGTAATCACCGTCAGACATGT30.24937655860349126No Hit
TGAAATTGTTTCCATTAATTCATTATACTGTTTTAAAATCAAAAACCAAA30.24937655860349126No Hit
CAGACTGTTCGGACTCTTCAGGTCGTTGTGAATGATCGGGGGCTCAGCAG30.24937655860349126No Hit
CCAGAACAGGACAACAATATGAGCAACTGCGCTTTATACAAAAACCCCTA30.24937655860349126No Hit
GTATATTCAGGGATGGTATAAAACAAACAAATGTTTTAATCAATTGTAAA30.24937655860349126No Hit
GTCGTCGTCAACTCCACTATCAAAAGTGTAAACAATTCATTTAAACCTAA30.24937655860349126No Hit
GGTGATATATTTTATTATGAATAATAATATAAATTATTAATAATTATTCA30.24937655860349126No Hit
ATTGTTTTTTTCTTTATTTTCTTTTTGCTAGTACGGAAGGTCCCTCTGAC30.24937655860349126No Hit
CATGTATAAACAAAAACGTATATTTATTTAGTTCTTTCTAGTTTTGTTAT30.24937655860349126No Hit
TATACATTCATATATTGGAACATAACAAATATAAGATGGTCGGCAAATAA30.24937655860349126No Hit
GTTTATTTCAAAAACTTTCAATCACCAATTTAAATAATTTAGCCTGCATT30.24937655860349126No Hit
GCCGCCTGTCTAAGGAGATATACATTGAAATAAAGTTAACCATAAGTTAT30.24937655860349126No Hit
GTACAGTCTCGGATTATTAGTATCTGCCCTTGCTACGAAAACGTACAGAT30.24937655860349126No Hit
CTTGATCCAAGATGTGTTAGTATGTCACGGTTGATCCAATGGTATTGCCG20.1662510390689942No Hit
GCCAGAGTTATCTCACTTAGTTATTTTAGTGGGTCTGGTAACTAGGAAGT20.1662510390689942No Hit
CTCCTGTATGGTACCCAAACCATCGTCAAAGTTCTGACTCCCACACACAT20.1662510390689942No Hit
GCATACAGCTACAAGTAATGTTGTTTACTGCATCAGTGCAATTCCCGCCG20.1662510390689942No Hit
CGACATAATAAATGTTTAAAACCAAACGAACCAATCTTCTGACCTGACAA20.1662510390689942No Hit
CCAGATATTCAGCCCCGTGGAAGAATGCAATTGAGGCAGCCGTGCAAAAT20.1662510390689942No Hit
CATGTATAAGATCTCTCGTAAACCATGATATCAAGTCAACATTTACTCTT20.1662510390689942No Hit
TTTTTTTTCCCTGACAACTACTAAGTAAATAACCCCAAAAATAGTAAGCA20.1662510390689942No Hit
TTTTTTTTCTGTTTTTTTCTTCTTTATTTCTTGCTTAGGAAAGGCTCTAC20.1662510390689942No Hit
TAAACAAAAAAGTAACAAAAAGGTTTTGACAATTATATTTTTAAATTTAA20.1662510390689942No Hit
CATCAGCACATTTTTGTCAACTTCATTTGCATAATAACATTTGCATAGAA20.1662510390689942No Hit
ATGTAATACACAAAAAAATAAAAGTATAAATGGCTTCCGCATGTTACCAA20.1662510390689942No Hit
TTGTCGACCGTTTAGGCTTCTTTGCTGCACCATCTTCGTCTTCCTCTTCA20.1662510390689942No Hit
ATCAACTGTTAGTTTAAAACAATTATCTGGAACATTTGTAAGAGCCAATT20.1662510390689942No Hit
TTTTCATTGAGATTGTGTGATATCTTGCAATATCTCGTTTTTAATTATCT20.1662510390689942No Hit
CGCAAAATTTCATTAAGACCCGATCACTAACCCAAGTGACAGGACACCGA20.1662510390689942No Hit
TGGAAAATAGCATTGTACTACTTGCTACATAGTAGATTAAATAATACAGA20.1662510390689942No Hit
CAAATAAACACCACTGCCAAAGATGCAATAAAAAATTAAGACTTTAAATT20.1662510390689942No Hit
GCACGAGTTATCTCACTTAGTTATTTTAGTGGGTCTGGTAACTAGGAAGT20.1662510390689942No Hit
GAAAAATATAAAAGCACAACTATGGAAAACAAGTTTAGTTGATGGAAATA20.1662510390689942No Hit
AGTTCTCTTGTTTTATTACTATTTCTATAAGTCATATACTATTCTAATGT20.1662510390689942No Hit
CCAGTCGATCCACAACATTTCAGCCAAACCTGGATGTAGTCCCACGCCTT20.1662510390689942No Hit
GGCCCTAAGAAATTTCTGGTCCTTGGCATTCCTAGCTGCGATGATTGGTT20.1662510390689942No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1662510390689942No Hit
GCTATATCATCCTACACAACAATAGAGTGGTGATACATAAATTGTTATCA20.1662510390689942No Hit
CGAGCTAACAGACTTTGTAAGCCTATACACTTTATCAAGTCCATAAACAC20.1662510390689942No Hit
AAAACCACGAAAGGTAACTAAGACCACAACTTTGTGTTTTACAAAAACTT20.1662510390689942No Hit
TGAACTTGTTTCCATTAATTCATTATACTGTTTTAAAATCAAAAACCAAA20.1662510390689942No Hit
GAACAAGTCAGAATGACGATGTGAAGGTTGTCAATCAGTATTTAAGTGCT20.1662510390689942No Hit
TATTTTTGTCTTCAGTTGTAATTTTGACTTTAATTTTCATTTCCTAAAAA20.1662510390689942No Hit
CAGCCTGTTTATTATCTCTATAGGAAAGTACATACTAAAGCTGTTTCTGC20.1662510390689942No Hit
TTGGCCAGTTCAAGTAATTATCAACAATAATATGCTTATATAACTACTGT20.1662510390689942No Hit
AATAACTCACTTTGAGCTTTGCTCAAGTGAGATAAAAACAAGAACCATGC20.1662510390689942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)