FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-4_S31_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-4_S31_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015
Sequences flagged as poor quality0
Sequence length41-151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC302.955665024630542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3940886699507389No Hit
GACCCAGTGGAGGCCATGCTGTTGTCCTGAAATGGTCCATGTGGAATTTA20.19704433497536944No Hit
GTCAGATCTAGTGTATGTGTTTTATATGCAGGCATTATAGTGTCTCTGTG20.19704433497536944No Hit
GGATTGTCAACCTGTCACGATTACATCGTTCCTCCGGAGGGCACTCGACA20.19704433497536944No Hit
GTTTAATGGGAATTTGATGCGGGGTTTGAGGTGTCGTAATAGGAGTAGTA20.19704433497536944No Hit
AGCAAAGCACCCAAAATTCAGCGACTTGTCACCCCTGTGGTTATCCAGAG20.19704433497536944No Hit
TGGAAGAAAATAAAAAACACGATTTTTATTGGTTAATCAATTTTTATAGA20.19704433497536944No Hit
CATACATGTACGTAAAGTGAATCTAAGAATTTGTTTTTCACCCCGACTTG20.19704433497536944No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCC20.19704433497536944No Hit
GTCACGTAGATCAACAAAATCAAATATAAGACAGAAGAATAAGATCGTCA20.19704433497536944No Hit
CCAGTGCCCAGTCCAAACAAGAAATCATTTTTGTAGCCGAGGCTGACTCC20.19704433497536944No Hit
GTGATAATGGGGAAAATACGCGACATTAAATGTTGAAGTTGCAAGAGCTA20.19704433497536944No Hit
TATACATAATGACATGAAAGCAAATTCACATCAATACTAGTCAGTTTATT20.19704433497536944No Hit
ATTTAAGCAGTTGCAAAAATGGCCTCTGAGGGATGATAGCTCCAACTGGT20.19704433497536944No Hit
CCTCTCTCCCATTTTTCTGTTCCACTTCTACATTTCTTTATTATTCCTCT20.19704433497536944No Hit
AAATACTAGATGTAAACCATTTTAAATATTAAAAATATCGCATCGCTTGC20.19704433497536944No Hit
CACCCAGGATGATCGCCAGAGAATCTTAGATGGTCTTAAGAAAAACTGGG20.19704433497536944No Hit
GTTTGAACCCAAGATTTTGGATATTAAAGATGAGGATCTCAGAGAAAGAT20.19704433497536944No Hit
TTTGGTGGTCACAAATCAACAAACAGAACATTCAGTCGGTCTTCTATCAC20.19704433497536944No Hit
ATAGAGTTGTTTATTACATGATTATTGTTTTGTCATTGGGTCAGGTAAAG20.19704433497536944No Hit
GCTCTAAGAAAATGGAACATTTTTTTGTTTAGAGCTATCTATACTCGTTG20.19704433497536944No Hit
CTGAAAATTGATTTCATATAATTATGCATTATATTGATGTATTATTTTCA20.19704433497536944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)