FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-4_S31_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-4_S31_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015
Sequences flagged as poor quality0
Sequence length41-151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC383.74384236453202No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC201.9704433497536946No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3940886699507389No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2955665024630542No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.2955665024630542No Hit
GAAACAAGCAAATATAGGAGAGGAAGAAGGACCTGTAGGAGAGGAATAAT20.19704433497536944No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.19704433497536944No Hit
TCCATCCAATGCCCTTGTTTACCCTTATTTTTGGTTACCATTTGATGTAC20.19704433497536944No Hit
GCCCTATAAACATAATCGTACCTTCAATATCACTGATGCCCAATTACCTT20.19704433497536944No Hit
CCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.19704433497536944No Hit
GTTTATTCAGAAATGAAAGGGTATTGACTTTAGATCGAAAGAAGCTAACA20.19704433497536944No Hit
CTTTTATGACAAAATAGCAATTCTCTTTGAAACTTTTTAACGAATTTACA20.19704433497536944No Hit
CACGCCGATACCGGCTCCTACCAATACCGCGTGGTCGGTGTCAAACACCT20.19704433497536944No Hit
GGATATTGCCTTATCAATATATACGTATTTACTGATGGTATTTTTTCTCT20.19704433497536944No Hit
AGATTATTTTGGTTTTAGCACCTCAGAACCATACCTGTACTTAAAAATTT20.19704433497536944No Hit
ACGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.19704433497536944No Hit
GCATTCTTGAATATTGTAGATAGAAAATCAACACTTTGTTAACCACATTA20.19704433497536944No Hit
CTCTTTCTAATTGCTTCATTTCTGCTTCCATTCTCTCCTTTCTGTTCTTT20.19704433497536944No Hit
AAACATTATATATGCCTGAATATAATACACAACACACTAGATATAACTGC20.19704433497536944No Hit
ACCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.19704433497536944No Hit
CCCCCCCCCCCCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCCCC20.19704433497536944No Hit
ACTCACTTCTCTCCCTTCCTTTTAGCAGCAATACCACTTAGTAATCTATT20.19704433497536944No Hit
GGAAAGTTTATTCAGAAATGAAAGGGTATTGACTTTAGATCGAAAGAAGC20.19704433497536944No Hit
AAATTACAATAATTTAATAAGTTAGTACTCTTAACTACATCATAACAAAG20.19704433497536944No Hit
AAGTAGAGTAACATTCTGTTCCTCCAATTCCTTTTTGATCTTTTCAATCT20.19704433497536944No Hit
GTCCGAGTTTCATATTTACTATGTTTGTCACTACTTTCAACCAACGTCAG20.19704433497536944No Hit
AATATGAACAACATTGAATGATCCGAACAATGACCCTCCACACACCACTG20.19704433497536944No Hit
CTCAAATCCAAGCTACGATGTCAGGCCCAAAATATGGAACAGCTCTAAGT20.19704433497536944No Hit
TTCGAAGTCAATGTCTGTTTCAACAGCAATGGCCAGGAGCCTCTTGAATC20.19704433497536944No Hit
CTCTAGTTCAGTCACCCAAGACTAAATCTAAAATCATAGAAGAACCTGTC20.19704433497536944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)