FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1124.239212717638153No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC973.671461014383043No Hit
TTACAACAAGACAATCTATGGAATGATACTAGTATAGTTCGAATCTGGTT80.3028009084027252No Hit
AGGACTTTAGCTTCTGTTTTTATTGGGGTAGTGTTAAGGATTAGTGTTTT60.22710068130204392No Hit
CGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTC60.22710068130204392No Hit
GGATAACATCGTGTCACGTGACCAAGAGAACGCATTCGGATCACCTCTCG50.18925056775170326No Hit
TGTTGAACAGCAATTTTTGGAGATAGGCCTACAGTCTAACGTTTCATCTG50.18925056775170326No Hit
TGTGGGTCTGTGACATGAGGCTCCAGTGTGGCAGTGCTCGAAATGCTGTC50.18925056775170326No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.18925056775170326No Hit
TGGGAATGAGTACTTCTCTGACAATGGGTGTATCGCTGTTCAAACAAACT50.18925056775170326No Hit
GTGCAATGTATACATGTGATACAGGACTTTGCGTAACAACTGGCAGAGAC40.1514004542013626No Hit
CGGGGTTTGAGGTGTCGTAATAGGAGTAGTAACGAATTGCTGGAGAGTTT40.1514004542013626No Hit
TTTAAGAGCATTTTATTTTTAAAATACATTTTAATTTTGGTTTTATTTTT40.1514004542013626No Hit
CGCAGTTCTAATATCTAGTTTTGTCACCGACATGAAATAGTCGTGCACTA40.1514004542013626No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA40.1514004542013626No Hit
GAAGGCAAGGAGCTGGAATTTTACATGAAAAGGATGAAGTCCAAGAAGGG40.1514004542013626No Hit
TGAAAAGGACGAGGAGATCGAGGCTGTAAGGAAGAGCGGACAACGCGCCA40.1514004542013626No Hit
GTTTGATCCACCGCCACGGCAATGTGTTGTGTCGTTGAGCTGAAAGACAC40.1514004542013626No Hit
TTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1514004542013626No Hit
GTGTCCACAATTTATCTGTATTATAAAAGTAGAGTCTATCAACAACTGAT40.1514004542013626No Hit
CGAAATTGGGTTTTATTCTTAGTTTTCTTTATGTGTTTTTTTTGTGTGTG40.1514004542013626No Hit
CGCCGTTCCTGGGCGGTTGAATATGTTGACTTTAAAGAGAAATCATGTTG30.11355034065102196No Hit
CTAGTCAGTCGCTTTCAAATCCACATTTTAAACTTGTAACCACACTTGTA30.11355034065102196No Hit
TAGTTTAAGCAGGACACCCTTCATTGTTTATAATCCACCTATTTTGAATA30.11355034065102196No Hit
CGTTCACGTTTAAGCATACTTGGTGGGTTGGTACTACTATTTTTTTATTG30.11355034065102196No Hit
GAGCTGGCATTTGAGTTAAGTGATGACTGGAAAATTGATTATGAATCGTA30.11355034065102196No Hit
TATGGAGGCTATGGTCGTGGAGGTTATCGAGGCTATAGAAGAAGCAAATA30.11355034065102196No Hit
GCATCTTTCAGGAACATCAAGTCGATCGCTGAGTGTCTTGCTGATGAACT30.11355034065102196No Hit
CCAATGCCCGTATGCCACAGAAGGTAATCTGGATGAGACGTATGCGAGTC30.11355034065102196No Hit
TTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11355034065102196No Hit
TGGAAAAGAACGACTATAAACGCTTCGGTGAACTGATGGTGCAGAGTCAC30.11355034065102196No Hit
GCCGCAATTCGAAGTCGTACATGTGTTTTACTATATCTCATCGTGTTACT30.11355034065102196No Hit
GCAAAAGTTGAGAATGATGTCTTCCATGGAGAAGAAGTGGAAAACTTGTA30.11355034065102196No Hit
GCTCGAGTCACGTGATCATTTTAAGCTTTTTGACAAAACTTTAAGCAAGG30.11355034065102196No Hit
GCAAAGTTGACAGTCGCTGATTTTTTATATATGGGTTACCGGTAAATGCA30.11355034065102196No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11355034065102196No Hit
TTTTTTTCGTGTAAACTATACATTTATGTTATATGCTATTTTTAAGTATT30.11355034065102196No Hit
AAATGCCTTAAATAAAAAAATGATTGTTAAAATGGATAAAAAGCATTACA30.11355034065102196No Hit
TGAAGGGATTCAGCTCCTTCTTCAAAAAGTCGTCTGATGAGGAACGTGAG30.11355034065102196No Hit
TGGTGCCCCTAGCTTGTGTTTTATTTTTGCATATGTTTTATGGTTGATCT30.11355034065102196No Hit
GCTACATTCAAATTCAAAAAATTTTTTTGGCCAATTGAGGTAAAAAATAT30.11355034065102196No Hit
CGGGCTTAGGTATGCACCCTGTTTGAAGGAAGGGGCAATTTTTTTTAATG30.11355034065102196No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG30.11355034065102196Illumina Single End PCR Primer 1 (100% over 50bp)
AGGAGTTTTAGCATTTCTAGGAGCCAAACACGATGGCTAAGCTGTGGACA30.11355034065102196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)