FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721
Sequences flagged as poor quality0
Sequence length31-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1096.333527019174897No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC945.461940732132481No Hit
TTACAACAAGACAATCTATGGAATGATACTAGTATAGTTCGAATCTGGTT80.4648460197559558No Hit
AGGACTTTAGCTTCTGTTTTTATTGGGGTAGTGTTAAGGATTAGTGTTTT60.3486345148169669No Hit
CGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTC60.3486345148169669No Hit
TGTGGGTCTGTGACATGAGGCTCCAGTGTGGCAGTGCTCGAAATGCTGTC50.2905287623474724No Hit
GGATAACATCGTGTCACGTGACCAAGAGAACGCATTCGGATCACCTCTCG50.2905287623474724No Hit
TGTTGAACAGCAATTTTTGGAGATAGGCCTACAGTCTAACGTTTCATCTG50.2905287623474724No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2905287623474724No Hit
GAAGGCAAGGAGCTGGAATTTTACATGAAAAGGATGAAGTCCAAGAAGGG40.2324230098779779No Hit
GTGCAATGTATACATGTGATACAGGACTTTGCGTAACAACTGGCAGAGAC40.2324230098779779No Hit
CGGGGTTTGAGGTGTCGTAATAGGAGTAGTAACGAATTGCTGGAGAGTTT40.2324230098779779No Hit
TGAAAAGGACGAGGAGATCGAGGCTGTAAGGAAGAGCGGACAACGCGCCA40.2324230098779779No Hit
GTTTGATCCACCGCCACGGCAATGTGTTGTGTCGTTGAGCTGAAAGACAC40.2324230098779779No Hit
TTTAAGAGCATTTTATTTTTAAAATACATTTTAATTTTGGTTTTATTTTT40.2324230098779779No Hit
TTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2324230098779779No Hit
CGCAGTTCTAATATCTAGTTTTGTCACCGACATGAAATAGTCGTGCACTA40.2324230098779779No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA40.2324230098779779No Hit
TGGGAATGAGTACTTCTCTGACAATGGGTGTATCGCTGTTCAAACAAACT40.2324230098779779No Hit
GTGTCCACAATTTATCTGTATTATAAAAGTAGAGTCTATCAACAACTGAT40.2324230098779779No Hit
CGAAATTGGGTTTTATTCTTAGTTTTCTTTATGTGTTTTTTTTGTGTGTG40.2324230098779779No Hit
TATGGAGGCTATGGTCGTGGAGGTTATCGAGGCTATAGAAGAAGCAAATA30.17431725740848344No Hit
GCATCTTTCAGGAACATCAAGTCGATCGCTGAGTGTCTTGCTGATGAACT30.17431725740848344No Hit
CGCCGTTCCTGGGCGGTTGAATATGTTGACTTTAAAGAGAAATCATGTTG30.17431725740848344No Hit
CCAATGCCCGTATGCCACAGAAGGTAATCTGGATGAGACGTATGCGAGTC30.17431725740848344No Hit
CTAGTCAGTCGCTTTCAAATCCACATTTTAAACTTGTAACCACACTTGTA30.17431725740848344No Hit
TTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.17431725740848344No Hit
TAGTTTAAGCAGGACACCCTTCATTGTTTATAATCCACCTATTTTGAATA30.17431725740848344No Hit
TGGAAAAGAACGACTATAAACGCTTCGGTGAACTGATGGTGCAGAGTCAC30.17431725740848344No Hit
GCCGCAATTCGAAGTCGTACATGTGTTTTACTATATCTCATCGTGTTACT30.17431725740848344No Hit
GCAAAAGTTGAGAATGATGTCTTCCATGGAGAAGAAGTGGAAAACTTGTA30.17431725740848344No Hit
GCTCGAGTCACGTGATCATTTTAAGCTTTTTGACAAAACTTTAAGCAAGG30.17431725740848344No Hit
GCAAAGTTGACAGTCGCTGATTTTTTATATATGGGTTACCGGTAAATGCA30.17431725740848344No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.17431725740848344No Hit
TTTTTTTCGTGTAAACTATACATTTATGTTATATGCTATTTTTAAGTATT30.17431725740848344No Hit
AAATGCCTTAAATAAAAAAATGATTGTTAAAATGGATAAAAAGCATTACA30.17431725740848344No Hit
TGAAGGGATTCAGCTCCTTCTTCAAAAAGTCGTCTGATGAGGAACGTGAG30.17431725740848344No Hit
TGGTGCCCCTAGCTTGTGTTTTATTTTTGCATATGTTTTATGGTTGATCT30.17431725740848344No Hit
GCTACATTCAAATTCAAAAAATTTTTTTGGCCAATTGAGGTAAAAAATAT30.17431725740848344No Hit
GAGCTGGCATTTGAGTTAAGTGATGACTGGAAAATTGATTATGAATCGTA30.17431725740848344No Hit
CGGGCTTAGGTATGCACCCTGTTTGAAGGAAGGGGCAATTTTTTTTAATG30.17431725740848344No Hit
AGGAGTTTTAGCATTTCTAGGAGCCAAACACGATGGCTAAGCTGTGGACA30.17431725740848344No Hit
TGTGTCCACAATTTATCTGTATTATAAAAGTAGAGTCTATCAACAACTGA20.11621150493898895No Hit
GTCGTCTTTATTCATATAAAATGTCATATTTATAGACAATGGAATATATT20.11621150493898895No Hit
TGATTAAGGTGTCAGAAATAGGCGCATAGGGCCTATGTATAAGCGGATAT20.11621150493898895No Hit
TTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11621150493898895No Hit
CAAAACATGGCGGAGCAGAAAGATAAAGAAAAGAACCCCATGCGGGATCT20.11621150493898895No Hit
ACGAGCAGCTCTCCGTTGCCGAGATCACCAACGCTTGCTTTGAGCCAGCC20.11621150493898895No Hit
CGTACTTTCAGAAAGTACATGTACAGAGGGGTCGATCTTGACCAACTTCT20.11621150493898895No Hit
TGATGATGATGATAAGGATGATAATGATGGTGACGATGACAATGCTGATG20.11621150493898895No Hit
TTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11621150493898895No Hit
CGCGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTT20.11621150493898895No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11621150493898895No Hit
TGTGGGTGGTTTAACCATAAAAAATAGTAAGGGGGGTAATGTCGTGTTTT20.11621150493898895No Hit
CCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.11621150493898895No Hit
GTCACCGATTGGGCAGCAGAGACCATGCCACCACCGACATTGTCAGGAGG20.11621150493898895No Hit
GTCCTCCATCACTGCCTCACTCAGATTCGATGGTGCCCTCAACGTCGACT20.11621150493898895No Hit
TTATCAGATACATTAGTTTAATTCATTGTTTTAATCGGGGTGTTTAAGTG20.11621150493898895No Hit
CGTTCACGTTTAAGCATACTTGGTGGGTTGGTACTACTATTTTTTTATTG20.11621150493898895No Hit
CTCTTTCCTGCCAATAAATGCTACCATACCTATTCTAAGGAAAATAAAAT20.11621150493898895No Hit
GCTATCAGTGCCGCAGGAGTAATTACTGCCGCAAACAAACTATATCGCCA20.11621150493898895No Hit
CGGAAGTTTTCTTTTTTGTCTCATTTTTTTGGTCCTTTTTTCATTTTAGG20.11621150493898895No Hit
GATATCTTAATTGCTTTTTTAAAGTTAAATTTGGTGGATTGTACTGTATA20.11621150493898895No Hit
TTTTTGTCTTTATCAAGACCCTGTATAGACTAAAATTAACTATGTGACAT20.11621150493898895No Hit
TTTGAGGAGGGTTTGCGGTAAGGGACGCAACGTTGAAGCGGTTTTTTGTA20.11621150493898895No Hit
TGTATATTAAATGATTTCATGTCGTCTCGTATTAGCATGCTTGCTTCAAG20.11621150493898895No Hit
CGAACTGGCACCTACAGGCAGTTGTTCCACCCAGAACAGCTGATCACCGG20.11621150493898895No Hit
CGTCAGTGAACGTACGGACAAATTTTCGACAAACTCTTTAAAAAACTTTT20.11621150493898895No Hit
CGTGGTACAGTCCACAGCCTTTGGACATTAGCACATTGTAGACATTGCTC20.11621150493898895No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11621150493898895No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTT20.11621150493898895No Hit
ATTGTGTCATTTTTATTGGTGATCTATTATCAGAATAAGGAATCTTTGGG20.11621150493898895No Hit
CAAAAGGGCTCTTGGAAAGGTTTTACATTATTCTAAATATTTTTTTTTTC20.11621150493898895No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC20.11621150493898895No Hit
TTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11621150493898895No Hit
GGGCTACTCTCAATGACATCATGGCCAAGAGAAATCAGAAGCCTGAAGTC20.11621150493898895No Hit
GCCATATTTGCTATTTCTATGGTTTCAGAGCAAATTAACCTGAATTGAGC20.11621150493898895No Hit
CCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.11621150493898895No Hit
GCGGGTATTATCCTTACTATCGACGCTATGGCCGTCATGGACACCATCAT20.11621150493898895No Hit
AGAAGAAGCAAATATTACTACTAGAACACTCGACGCCTATAAAGACCCTT20.11621150493898895No Hit
GTAGAGTCAGGGATATTTATATGATCTGTTGTACAAATAGACAACAGGTT20.11621150493898895No Hit
CGTCAATCTTAGGTTTCAGTTTTTTTTCATAAATATGGTATGGATTAGTT20.11621150493898895No Hit
CCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCC20.11621150493898895No Hit
CGTTCCTGGGCGGTTGAATATGTTGACTTTAAAGAGAAATCATGTTGGTG20.11621150493898895No Hit
GGGCTATCAACCTCCAGTTCAAGACCAGTATCCAATGGGATTTAAGCCAT20.11621150493898895No Hit
CTGGATTTAGTAAAACTGCATATGGTGTCATTGATTGCATTATGGATAAA20.11621150493898895No Hit
CGGGCATTTGTGTGTGATGGCTTCCGCACATTGATTTTTATAAAGATGGG20.11621150493898895No Hit
TTGATACCCCATTGCTGAAGATGCCGGAATTATTGGACAAATACCCTAAA20.11621150493898895No Hit
CGAAAGTATAACCACTTTAACGATGGTGGTGACTGTGGACTCAGAGACAC20.11621150493898895No Hit
GCACAATCCAGGAAAGGGTATTTCCCAATCAGCTCTCCCATACAGAAGAT20.11621150493898895No Hit
TTGTATGTATGTGCATTTACAATCTTTTTTATATACATTTATTATGAATC20.11621150493898895No Hit
AATCTTGTCTGTGCTTCAATTCTTCTCTTTAACTCTCAGTTGAAATCACT20.11621150493898895No Hit
TGAAAACACGCTCTGCAGGAGAAAACAGGAAACAGAAGACAAGCGAGAAT20.11621150493898895No Hit
GGAAGCCGCGCCTGAGGAGGAATAGGCCATTTTACATTCGTTCAAATACT20.11621150493898895No Hit
GCACTATCTGTGTTAGTTAATTATGCTAATTTATTTAAGGCCATTGTAAA20.11621150493898895No Hit
GTTTGAGGTGTCGTAATAGGAGTAGTAACGAATTGCTGGAGAGTTTGTGA20.11621150493898895No Hit
GGAAGATCATTAAGGACGTCCGTTTCTCATTATGGCTGATACGGAAGGAG20.11621150493898895No Hit
TGTAGCTTCATATATAGGGTTTGCTAAACATTCTCTTTTTTATGCTTGGT20.11621150493898895No Hit
CGAAGAGGGAACTATGGAATGTTAGCACAATTTCCAAGACTTTTGTCGGT20.11621150493898895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)