FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1495.6396669190007565No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1164.390613171839516No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC351.3247539742619228No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC150.5677517032551098No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.45420136260408783No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.22710068130204392No Hit
TAACAAGAGACTTTCATTTTAAAGTATTGGATAATTGTAATTAGTAGACT60.22710068130204392No Hit
CAAAAGTCTACTCCTACATCTCCCCAAAGCCAATTTTGCAGGCATATAGC50.18925056775170326No Hit
CAGTAGGGCAAGTTGAAAACATGTATACCAATGATCTTGTGTAAACATCG40.1514004542013626No Hit
TTTTTTTATATATTTGCAGCTTTTAAACAAACATTGTTATTTCATTACTT40.1514004542013626No Hit
TTTTTTTTCTTTGTCCCAGTTTATTCTGCAGTATACAGAAACAACAGAGC40.1514004542013626No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.1514004542013626No Hit
TGGCAAACCGCAGATCTTTTAAAAAGAATGACGTCACTTCCTTGAAATCA40.1514004542013626No Hit
TTTTAGACACTCTGAGATTTATTGAAAAAAGAAGTCCTCAAATGCTCATA40.1514004542013626No Hit
CGTTGGCTCTGTTGGCGTTGTCAAGGGCAGCCTCGAGCTCGCGGATGTCG40.1514004542013626No Hit
TCCAAGATACACTTTTATTATACAATGGATAAAAAAGGTTTCATTCAAAT40.1514004542013626No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.11355034065102196No Hit
TGACTTGTCTATTTTTTAATCCTTTACTGTGCGACGTCGAATAACGCCCC30.11355034065102196No Hit
TTAACCACTCATCTTGATGTCGATTTTGTCATGTTTTTGGTGGATTTGAC30.11355034065102196No Hit
GATCCGTACACGCCTTCCACTCCCATAGCCGCAACTACAAGCTCGTCCAA30.11355034065102196No Hit
CCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.11355034065102196No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11355034065102196No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11355034065102196No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA30.11355034065102196No Hit
GTTAACATGTCAGTTGACGGCACATAGTTATTATAACAGTTTATCTGTTG30.11355034065102196No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.11355034065102196No Hit
TGCCCTTTTAGGATTTTCGATCTAAATACTTCTTTATTCTCGATCTTACT30.11355034065102196No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11355034065102196No Hit
TTTTTTTTTCCAAGATACACTTTTATTATACAATGGATAAAAAAGGTTTC30.11355034065102196No Hit
AAAATTTGTAAACTTATAAATACTTCTGCTCCATATATTTACATTTAAAA30.11355034065102196No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC30.11355034065102196No Hit
GCCTTGTCAAAATAATGTATGCTAATTGTTTTGTTTGTTTTTGTTTTTTT30.11355034065102196No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11355034065102196No Hit
TCTTGGTCTGGCTTCTGGATGGGCTGCAGAACGACGCGGCCGCCTCTCTT30.11355034065102196No Hit
TTATTTGTTTCTGTTTATTTTTGCAGAAATTTCAAGCATTTTCAACTTTA30.11355034065102196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)