FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-3_S23_L003_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721
Sequences flagged as poor quality0
Sequence length31-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1488.599651365485183No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC925.345729227193492No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC201.1621150493898895No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.6972690296339338No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC100.5810575246949448No Hit
TAACAAGAGACTTTCATTTTAAAGTATTGGATAATTGTAATTAGTAGACT60.3486345148169669No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.2905287623474724No Hit
CAAAAGTCTACTCCTACATCTCCCCAAAGCCAATTTTGCAGGCATATAGC50.2905287623474724No Hit
TGGCAAACCGCAGATCTTTTAAAAAGAATGACGTCACTTCCTTGAAATCA40.2324230098779779No Hit
TTTTAGACACTCTGAGATTTATTGAAAAAAGAAGTCCTCAAATGCTCATA40.2324230098779779No Hit
CGTTGGCTCTGTTGGCGTTGTCAAGGGCAGCCTCGAGCTCGCGGATGTCG40.2324230098779779No Hit
TCCAAGATACACTTTTATTATACAATGGATAAAAAAGGTTTCATTCAAAT40.2324230098779779No Hit
CAGTAGGGCAAGTTGAAAACATGTATACCAATGATCTTGTGTAAACATCG40.2324230098779779No Hit
TTTTTTTATATATTTGCAGCTTTTAAACAAACATTGTTATTTCATTACTT40.2324230098779779No Hit
TTTTTTTTCTTTGTCCCAGTTTATTCTGCAGTATACAGAAACAACAGAGC40.2324230098779779No Hit
TTTTTTTTTCCAAGATACACTTTTATTATACAATGGATAAAAAAGGTTTC30.17431725740848344No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.17431725740848344No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.17431725740848344No Hit
TGACTTGTCTATTTTTTAATCCTTTACTGTGCGACGTCGAATAACGCCCC30.17431725740848344No Hit
TTAACCACTCATCTTGATGTCGATTTTGTCATGTTTTTGGTGGATTTGAC30.17431725740848344No Hit
AAAATTTGTAAACTTATAAATACTTCTGCTCCATATATTTACATTTAAAA30.17431725740848344No Hit
GATCCGTACACGCCTTCCACTCCCATAGCCGCAACTACAAGCTCGTCCAA30.17431725740848344No Hit
GCCTTGTCAAAATAATGTATGCTAATTGTTTTGTTTGTTTTTGTTTTTTT30.17431725740848344No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17431725740848344No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17431725740848344No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17431725740848344No Hit
GTTAACATGTCAGTTGACGGCACATAGTTATTATAACAGTTTATCTGTTG30.17431725740848344No Hit
TCTTGGTCTGGCTTCTGGATGGGCTGCAGAACGACGCGGCCGCCTCTCTT30.17431725740848344No Hit
TTATTTGTTTCTGTTTATTTTTGCAGAAATTTCAAGCATTTTCAACTTTA30.17431725740848344No Hit
TGCCCTTTTAGGATTTTCGATCTAAATACTTCTTTATTCTCGATCTTACT30.17431725740848344No Hit
TGCACTTTTAGGATTTTCGATCTAAATACTTCTTTATTCTCGATCTTACT20.11621150493898895No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11621150493898895No Hit
GCCATTTTAAGTACATTTCATGCCATTTTTTTTGGTTGTTGTATGGTGTT20.11621150493898895No Hit
GTTCATGCGGCCACGACCCTTTTTATTTTCATAAAAAAACAATTTTTTAT20.11621150493898895No Hit
AAATATGACATTTTATTTTGAATATTTTAAACGAAAGTCCCATATGCCGT20.11621150493898895No Hit
GGTTCATCAATGATATAATCTAATTTTGATATTTTCATGTCATAATGAAC20.11621150493898895No Hit
AAACAAGTGAAATAGTAACAGATAAAAAACATTATCAAGTTTTAAATAAA20.11621150493898895No Hit
GCCCTTTACACTTCCTTCTCTTCGAACTACTTAATTATATTTCCTCGCCC20.11621150493898895No Hit
TAAAAAACATATTAAGGTAATAGAACAACCTAACTACTGACCTAAAAATT20.11621150493898895No Hit
CAACTCTTCAAACTGTTGACCTCTTGCCCTTTATACAAACATTAAAACTA20.11621150493898895No Hit
CAATATAAACATAATTCACCCATTGTTGATATGTGGTGAATTAATGCTTT20.11621150493898895No Hit
CGCATTGCTCCCAATAAACCATACTGAGAATTACATCTTCACCCTACCAC20.11621150493898895No Hit
GTCAAACTTGAGAGAATTTCAAAACTTAACGTTCCACATTTTCGGTATAG20.11621150493898895No Hit
GACGAAGTCTCACAAACATAATCAACGATCAACATGGGCATTTTTCTTTC20.11621150493898895No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC20.11621150493898895No Hit
TTCATTAAGTATTATTTATTCAAGTCATCTTTCAACAATAGTACATTTGA20.11621150493898895No Hit
CTCATGGTACAGATGTTTGTCGATCTTCTTGGCCTCGCGGTATCTCTTCA20.11621150493898895No Hit
CTTGAATAATAGAGCATATACATAAACGGGTGAACATCACAGAGTTGCTG20.11621150493898895No Hit
TGGTTATGGTTCTCCGTGTACACGGCACCGAATGCTATAAATCCTATCCG20.11621150493898895No Hit
TTGCTGTTCAGTGGACAGCACTGCAATGCATTCCCAAAGTTTCTGTGTCA20.11621150493898895No Hit
CTTTGTTTGCATCTGCTTGGCAGCCTTCTGTTTTGGTCCACTGGACTTGA20.11621150493898895No Hit
TTGAAATGTCATGGATCACATTGTTTTCCATAATGATCAAATCTTAATTT20.11621150493898895No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC20.11621150493898895No Hit
TGTCTTTTGACAGGTATGAAGTCAAGCTGGGGATCGAACCCACAACCTCT20.11621150493898895No Hit
GGAGGTGCTTCATACTTGTAATTTTGCTTTACTCTTGGATTGGAATTCCA20.11621150493898895No Hit
CTCACAGTTGCAATGATTAATAAATAACTGTTAGTAATCTCTCAGATCAA20.11621150493898895No Hit
AGACATTCAAAATGACGAATGACAATACGTAGACACTACTACACTTCTGG20.11621150493898895No Hit
TTTATAAAGACGATGAAATATTTCGAACTTTTGTGTAGTTTGACTTGAAC20.11621150493898895No Hit
AAAAAAAAACAGTTATGACACATTATCTTATTGAAAAATACATCAGTTGT20.11621150493898895No Hit
GTGACAAACAGCGTTCCGGGCCTCTGTTTCACCAACAATTTACTTAAATA20.11621150493898895No Hit
CACAAATCTATTTTTTCTATTTATGTATGCTATATTTTTAGTATGGTAAA20.11621150493898895No Hit
CTTGGGGACAACATCACCTCTGTACAACATACAACAGGCCATGTACTTGC20.11621150493898895No Hit
CAGCTGTTTCCTTTACCTGTCAGTTATTAAATGTACTGTACACTTATCTA20.11621150493898895No Hit
ACTGGCTGTTGTCCTGTAAGCTGTTCCAACACTTTGGCGGCACGAGTCAA20.11621150493898895No Hit
CGTTGCGTCCCTTACCGCAAACCCTCCTCAAATCCAAGCTACGATGTCAG20.11621150493898895No Hit
GACTCGTAAAAGTACATGTAATTCATTTCTATTAAATACTTAATATCAAA20.11621150493898895No Hit
ATGGAGTCCTCGTGCAAGTCCATCATGTATCAGTGCAGTCTTGAGGACCT20.11621150493898895No Hit
TGGTGATCTCGGCAACGGAGAGCTGCTCGTGGTAGGCCTTCTCGGCAGAG20.11621150493898895No Hit
CTAGCCGTTATTTATTTACCATGGCGATATATAAACAAAGCACCATCTTT20.11621150493898895No Hit
CCAACCTCGGCTAAGTCGCTGAAATTTCAACTACCCTCAACCCTCAGCAA20.11621150493898895No Hit
GGGAACCACTGTCTTAACATACGTATAGCCATCATAGTGGGTGTGTTGGG20.11621150493898895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)