FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-1_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-1_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9398
Sequences flagged as poor quality0
Sequence length35-151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC650.691636518408172No Hit
GCCGCCATTGCTACCATCAAGACCAAGAGAACCATCCAGTTCGTCGACTG160.17024898914662695No Hit
TGAGTTTCAGACATGACTTTTGATTCATGTTTTAAGTTTTAGACATGACC150.15960842732496275No Hit
CTCCGTTGCCGAGATCACCAACGCTTGCTTCGAGCCAGCCAACCAGATGG140.14896786550329857No Hit
AGAAGGCCTACCACGAGCAGCTCTCCGTTGCCGAGATCACCAACGCTTGC140.14896786550329857No Hit
CCAGAATACCGAACGTAAAATATAATGTGCAAGAATACAGAACGTTAAAT140.14896786550329857No Hit
AATTATTCAAAAGCCTTCGGTATGATGGTGTCCAAAGGCGTCTATATTAG140.14896786550329857No Hit
CTCCATGTAATATGTAATAAGTCAATGTTTCTCCCACTGGGGAGTATAAA130.1383273036816344No Hit
GTGATTTATTGAAGTTGATGTGTTCTATTCTTAAAAATAGTTTTTTTTAT130.1383273036816344No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1276867418599702No Hit
AAAAATTTTGCACTAGTAGACATGCTTGCAATAATAAACTTTGACTTTCA120.1276867418599702No Hit
AATTTGATCCGCATTACACATAGTAAATGATTGTTTTTATCTGGGAAAGA120.1276867418599702No Hit
AAGTGATTGTATTAAAAGTGTTAATGTTGAATCGATTCAAAATAATAATT110.11704618003830602No Hit
CTTTTTTCAGATGGAAAAAAAGAAAAAAAGAAAAGAAGTGTTAAGTGATC110.11704618003830602No Hit
GTCCAAGCTGCCCTACCATTCAGATTCTATCATTGATTTGTGGCAGGAAA110.11704618003830602No Hit
TCTTCACAAACCTTCTTTAAGTTCTCCTCCATTTTTGTCTTGGCATTCCA110.11704618003830602No Hit
AGTGTTGACAACCTGTTCTGTTATATCAGGATGTTGACATTTTGTATCTA110.11704618003830602No Hit
AAAACTACAATCATGAGAGAAATAGTTCACATGCAAGCCGGACAGTGCGG110.11704618003830602No Hit
GGACAACATTGTACGATTCGTCGTCAAGAAAGCTCATAATTACGAACAGA110.11704618003830602No Hit
GTTTTGGTGAGAATTTCGCCCTTAGCGGTGCTGCTGCCATTATCTCAAAA110.11704618003830602No Hit
AGACGATAAACTCCTGTACCCAATGTTTTAAATTCTGACCAATCAGATAT110.11704618003830602No Hit
GGCGAGCTTATTTTAGTGCGGCAACTATGATTATTGCTATCCCTACAGGA110.11704618003830602No Hit
GCAGCTTCCGTTTCCACGTTGACTTACAGTGTTATGACCTGTTGTAGCTT100.10640561821664184No Hit
GTGCATTTCACCACCTTTATATAATATTTTTATACAAGTTCTTTAATGAG100.10640561821664184No Hit
CGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTC100.10640561821664184No Hit
TTCACATCCTTTAGGATAAATAAATAAATATGTGTGACGACGAAGTTGCC100.10640561821664184No Hit
GTTGTAGTTGTAAAACTGAAAATGGACAAAGATCGCAAGCGTATTCTTGA100.10640561821664184No Hit
GCAAAGTTTGCTTACATCTGCTCCATAAAATAAGGTAATTGATATATCTT100.10640561821664184No Hit
CTGGGTTGAGTGCCAGGTTTGAGTATGACTTAAAAAAAGTAATCGCTTTA100.10640561821664184No Hit
GGTTGCTGACCATACTGCTGACCTCCAGATTGACCTCCATACTGAGCATA100.10640561821664184No Hit
CTTTTCTATAATTTTTCAAAGAATTGAAAAAAAAAATCAATTACAGCAAT100.10640561821664184No Hit
CGAGAAGGGAGCTTGGAAATAATTACACTATCAATTAGGACTCTGTTTTC100.10640561821664184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)