FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-1_S7_L001_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-1_S7_L001_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8446
Sequences flagged as poor quality0
Sequence length38-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC630.7459152261425527No Hit
GCCGCCATTGCTACCATCAAGACCAAGAGAACCATCCAGTTCGTCGACTG160.18943878759175942No Hit
TGAGTTTCAGACATGACTTTTGATTCATGTTTTAAGTTTTAGACATGACC150.17759886336727446No Hit
CTCCGTTGCCGAGATCACCAACGCTTGCTTCGAGCCAGCCAACCAGATGG140.16575893914278947No Hit
AGAAGGCCTACCACGAGCAGCTCTCCGTTGCCGAGATCACCAACGCTTGC140.16575893914278947No Hit
CCAGAATACCGAACGTAAAATATAATGTGCAAGAATACAGAACGTTAAAT140.16575893914278947No Hit
CTCCATGTAATATGTAATAAGTCAATGTTTCTCCCACTGGGGAGTATAAA130.1539190149183045No Hit
GTGATTTATTGAAGTTGATGTGTTCTATTCTTAAAAATAGTTTTTTTTAT130.1539190149183045No Hit
AATTATTCAAAAGCCTTCGGTATGATGGTGTCCAAAGGCGTCTATATTAG130.1539190149183045No Hit
AATTTGATCCGCATTACACATAGTAAATGATTGTTTTTATCTGGGAAAGA120.14207909069381958No Hit
AAGTGATTGTATTAAAAGTGTTAATGTTGAATCGATTCAAAATAATAATT110.1302391664693346No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.1302391664693346No Hit
CTTTTTTCAGATGGAAAAAAAGAAAAAAAGAAAAGAAGTGTTAAGTGATC110.1302391664693346No Hit
TCTTCACAAACCTTCTTTAAGTTCTCCTCCATTTTTGTCTTGGCATTCCA110.1302391664693346No Hit
AGTGTTGACAACCTGTTCTGTTATATCAGGATGTTGACATTTTGTATCTA110.1302391664693346No Hit
AAAAATTTTGCACTAGTAGACATGCTTGCAATAATAAACTTTGACTTTCA110.1302391664693346No Hit
AAAACTACAATCATGAGAGAAATAGTTCACATGCAAGCCGGACAGTGCGG110.1302391664693346No Hit
GTTTTGGTGAGAATTTCGCCCTTAGCGGTGCTGCTGCCATTATCTCAAAA110.1302391664693346No Hit
AGACGATAAACTCCTGTACCCAATGTTTTAAATTCTGACCAATCAGATAT110.1302391664693346No Hit
GGCGAGCTTATTTTAGTGCGGCAACTATGATTATTGCTATCCCTACAGGA110.1302391664693346No Hit
GCAGCTTCCGTTTCCACGTTGACTTACAGTGTTATGACCTGTTGTAGCTT100.11839924224484963No Hit
GTGCATTTCACCACCTTTATATAATATTTTTATACAAGTTCTTTAATGAG100.11839924224484963No Hit
CGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTC100.11839924224484963No Hit
TTCACATCCTTTAGGATAAATAAATAAATATGTGTGACGACGAAGTTGCC100.11839924224484963No Hit
GCAAAGTTTGCTTACATCTGCTCCATAAAATAAGGTAATTGATATATCTT100.11839924224484963No Hit
GGACAACATTGTACGATTCGTCGTCAAGAAAGCTCATAATTACGAACAGA100.11839924224484963No Hit
CTGGGTTGAGTGCCAGGTTTGAGTATGACTTAAAAAAAGTAATCGCTTTA100.11839924224484963No Hit
GGTTGCTGACCATACTGCTGACCTCCAGATTGACCTCCATACTGAGCATA100.11839924224484963No Hit
CGAGAAGGGAGCTTGGAAATAATTACACTATCAATTAGGACTCTGTTTTC100.11839924224484963No Hit
CTAAACCAAATCAACAATTCTACTTTTTGATATCTGGAAATTTGCAGTTT90.10655931802036465No Hit
AAGCAATATTAGCTGCTCTCGAGCCCCCAGAGCCACAAAAGAAGAGGAAA90.10655931802036465No Hit
AAACAACGCCATCTTAGTGAAAACCAAACACAAGACACGCTTGAACAAGT90.10655931802036465No Hit
GTGTTGGTACGCCGTAAAGCCCTATTCATTCATGTAAATATAAACGGCAC90.10655931802036465No Hit
GTTTATGAAAGTTTGCCTAACCCCGAGGTCGGATATACTCTAACAGAATT90.10655931802036465No Hit
TGAAAATACAACACACGAGTGAAACTGCAGTAGCATATCAGTGGGAATAT90.10655931802036465No Hit
CCACAGTGGGTCAGAATGAAGACTGGAAACACTATCCGATACAATGCCAA90.10655931802036465No Hit
GTCCAAGCTGCCCTACCATTCAGATTCTATCATTGATTTGTGGCAGGAAA90.10655931802036465No Hit
TCCAAATATAAAGAAACCTTGTACAGCTTCACCATTGTTCAACTCAAATT90.10655931802036465No Hit
AAAAGATTCATTATGAACAGGAAGTGAAGAGGAACAGGGAGCGCGTGTCC90.10655931802036465No Hit
CTTGCCGAAAAGGACGAGTTTGAAGACAAACAGAAGGAGTTGGAAAAAAT90.10655931802036465No Hit
GCCATGGGATCCAACTGGCAAGATTGGACCCAAGCGCCCACTGCCAGATC90.10655931802036465No Hit
GCGGAACAGACCGAGAGAGCGTTTCAGAAGCAGTCATGTGTATTTTTAAA90.10655931802036465No Hit
TTTGAATTGTTTTTTGTGTATTTTTGTTGAATTTGTTTTTCATCCAAAGA90.10655931802036465No Hit
TTACGGTTGGCATAAATATTGATAGGCGAGCTTATTTTAGTGCGGCAACT90.10655931802036465No Hit
CACATTCCCATCAACAACATCATTGTTGGATCCTGATTAAAAAAATATCA90.10655931802036465No Hit
GGTGATCAACGTTGCAGGCTCATTCTTGATGATATAACTTTTATTTTGAT90.10655931802036465No Hit
TTGGACGAGACAGTGGTGATATAATTTGCAGTGCAATGAAATAGACAGAT90.10655931802036465No Hit
GTTGTAGTTGTAAAACTGAAAATGGACAAAGATCGCAAGCGTATTCTTGA90.10655931802036465No Hit
TCACCCTTTAATATATAATGATTTTAACACAAATTAAACAATTTGGATAT90.10655931802036465No Hit
GAGACTGCTCCGCTGGTAAAAAGGTTGGTCTTATTGCTGCCAGAAGAACT90.10655931802036465No Hit
TTTGAATCCTCAGTCTTGATCTCAGACTCGAGATATTGACGAATTTAGGT90.10655931802036465No Hit
GGAACTAGTTTATTTCGTTTCAGTACAGATCGTTCAAAAAAGCATTCAAT90.10655931802036465No Hit
GAACTTAAAAGAGGAATAGAGCATCTTGGCACATATTTCCTTAAAGATAA90.10655931802036465No Hit
CACATTGTATCACGAAATCACGAAAGAAAACAACAACAAAATTTAAGTGT90.10655931802036465No Hit
TACTGATGTACCTATAACTTACCATAGAATCTACTCTTAACATATCTCTG90.10655931802036465No Hit
GTCTGACTCAGCCTGAGGTCTTCCGCCACCAGTAAAACTGGATATGCCAT90.10655931802036465No Hit
GTCTGCCAACAAGATTTTGTTCATTGGTGCCCCTAGCTTGTGTTTTATTT90.10655931802036465No Hit
GCTTGGTGTGGCGTTTCTCTGAATTATAACAATGCAGAATATTCCTTTGC90.10655931802036465No Hit
GGAGAATGATGACATTAACCTACTGCAAGAGTTTGGTAGTCTGACAACGT90.10655931802036465No Hit
TCGGCTCATTGATTATGTTGTAAATTATTCCTGGAGGCATCTTTGGTCAC90.10655931802036465No Hit
GCGGTAGTTCATCTTATTTTTCTTCATCAGACAGGGTCAAACAATCCTTT90.10655931802036465No Hit
TCGAAACCTCCTCATATACATAAACACCTTTGATATAAACAATCCAGCAA90.10655931802036465No Hit
CCTGCACTTGGTGAATGATGTATTCGACCTTGACATGGTGAATGATATCT90.10655931802036465No Hit
GTCGTCTGCGGCGTTGTTTTTTATTGGCTTAATTGCTGCATTCCAAGTTG90.10655931802036465No Hit
AATTGCTCAAAAGCCTGACAAAATGTTCAATTTGAAGAAGTGGAATCTAG90.10655931802036465No Hit
CTTTTCTATAATTTTTCAAAGAATTGAAAAAAAAAATCAATTACAGCAAT90.10655931802036465No Hit
TCTGCACTCGTTGATAGGTTAAGCTGGCGTAATCTTTGTCCGGGTCGCAA90.10655931802036465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)