FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-124-1_S7_L001_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-124-1_S7_L001_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8446
Sequences flagged as poor quality0
Sequence length38-151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC530.6275159838977031No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.2131186360407293No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC180.2131186360407293No Hit
TTCAACCCTTTTGCTAATTGGTCATATATAAAGCAATGAAATATGTTTAA150.17759886336727446No Hit
GCGATGGCAGTTGTGTTGCTCAACATGCACACGGCTCTCTGGACCTTGGC140.16575893914278947No Hit
GCTTGTTCTTTTTTGGTGTGGTGTAATTCTTCTTCTTACGTTTCTTGGCA130.1539190149183045No Hit
TAACAAACACTTTCTAGAATTACCATCTGAGCTTGTTTTTGAGGTTGAAA130.1539190149183045No Hit
GATTAGTTATATATAGTTTTGTAAATACACGCTTTGAACTATAATGTGTT120.14207909069381958No Hit
GCGTTGACATCCTTGGGGACAACATCACCTCTGTACAACATACAACAGGC120.14207909069381958No Hit
TTTCAAACAAAAATACATTATTTTATTTAAATATGAAAACCATACACAAA120.14207909069381958No Hit
GCAATGGCGGCGTTGACATCCTTGGGGACAACATCACCTCTGTACAACAT120.14207909069381958No Hit
AACTTTACCAGTGTGTGATTATAGAACAGAACAGAATTTTGTACAATATA120.14207909069381958No Hit
GTCAACATTCACATATGACAGATTGACAACATTGAATCATCAAATGTTGA110.1302391664693346No Hit
GTGGCTTCGTTGTAGTACACATTTATTCTTTCCAACTGAAGATCTGAATC110.1302391664693346No Hit
CTCTGATAGCCTTCTTCAGGTTCTCAATGTTTCTCTCCATCTGTGTGATC110.1302391664693346No Hit
CTGCTACTCTCAAAAATTTCTTTCTACTCTACATAGCTATGGTACAACTG110.1302391664693346No Hit
CTGCTCTATTCTTCAGAATAACTGAAATAATATCCAAATCTGCTCAATTT110.1302391664693346No Hit
TTTTTTTATCAATCAAACAATTTTATTCATATAATTCCCCTATAACACGT100.11839924224484963No Hit
ACCCTTGTATGGCTTGTCAAGACGACCTTGTTTGATCATTTCATCTTGGT100.11839924224484963No Hit
TAAACATTTACTGTTCACTGGTACTTCCATCGGTATATTTGTGACAAGCG100.11839924224484963No Hit
GAGAGAACAGCACTTTCATGTAGTTACGTTCACATCAATGTCCATCCTTT100.11839924224484963No Hit
ACCTTGTTACCTAGGTAAAGCCCTTCTTCAACCACTCCGTTTACTTGCAT90.10655931802036465No Hit
CACAGTCTTTAATTCACTAAACAAATCACAGAACACTAATCCTCACATAT90.10655931802036465No Hit
CTTTAGTCTCATGGCCAATAGTTTAGCATATTCTGCAGCGTCTTCACGTT90.10655931802036465No Hit
GCCCACTGGCGGATTATATTTAGAATACCAATTTGAATTGATTAAGCATA90.10655931802036465No Hit
TTTCACTAGTGTGTTGTATTTTCATTAATATTCCCACTGATATGCTACTG90.10655931802036465No Hit
GCCCACTTCAGTTCCTTCTCAGTGATTTTTGTAGCCAGTACCAATCCTCC90.10655931802036465No Hit
AAAACTCTGTACATAAATTTTGCTTTTTGGAGAAATATTACAAGTGCAGA90.10655931802036465No Hit
GCTACATGTAAATGTACAATCAATAACAAGTCTGCTTATGTCTTATATTT90.10655931802036465No Hit
GGAATTGCAAAATCGGAAAACACTCTAACAGTTATGGTGCTGGTACACGC90.10655931802036465No Hit
GTTTAAAATTTTCCGCAGTCTTTGGTACAGTTTTCCCAAAGAGACCAATC90.10655931802036465No Hit
GGATGGTGTGCCAGGACGGTCTATGGGGTTTCCTGTTGTAGTGTGTGATA90.10655931802036465No Hit
TGAATAACAGCTCCAGCACATAAAAACATCAATGCCTTAAACAAAGCGTG90.10655931802036465No Hit
AATCTATCACTTGACTTAACACGTAAAAATGGAAATAAGCCGAGGGCAAT90.10655931802036465No Hit
CTCTCTTGCTCTGGGCTTCATCTCCTACATAGCTGTCCTTCTGTCCCATA90.10655931802036465No Hit
CAGTCATCTTGTTTGTTTTCTGATTGACAGCGGAGGCAAGCATCCATTAC90.10655931802036465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)