FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-6_S46_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-6_S46_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197
Sequences flagged as poor quality0
Sequence length45-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC544.511278195488721No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.835421888053467No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.5847953216374269No Hit
GTACTGACGGCAGATCAGATCTAGGTTACATTCGTCTATATTAGACTGCT70.5847953216374269No Hit
CCTCCTGTAACAACCTTTTGTTATCTCTTGTTTTATCAAGCTGACATATC60.5012531328320802No Hit
TTAGGTTCCCACTTTTTATTGAAACATACAAACGCTGTATAAAGTATTGG50.4177109440267335No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACC50.4177109440267335No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.4177109440267335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT50.4177109440267335TruSeq Adapter, Index 15 (97% over 40bp)
AGCCACTCAACCGTGCAAAAAAGTATCACTATGTTGTTCTTCATTAGCCA50.4177109440267335No Hit
CAACAATGTCTTGTTACCACATTATACATATATATTGAAAAATAAAATAT40.3341687552213868No Hit
GTTTTACAGGAGTCAAATGTCCTCTTATTTCAAATAAACTAGTACCTTAC40.3341687552213868No Hit
GGACCAGCCAAAAATAATAACCAAACCACGGGGAATAAAGTTCACAAACT40.3341687552213868No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCC30.2506265664160401No Hit
TAAATATTCACATTACAAATAACAGTAATTAAACATAAATGATCTATCCA30.2506265664160401No Hit
AAGATCGTGTAAGCAAATAAGAAATACCACTCTGGTTGAATGTGGAGAGG30.2506265664160401No Hit
AAAATATCTTGCTCAAGGTACCCTTGGCTGAGGATCAAACCCACAATCTT30.2506265664160401No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA30.2506265664160401No Hit
TTTTCTTTAAGGAGTCCAAGGTTGTTGAGGAAGTCGCGTATATGACCTAT30.2506265664160401No Hit
GTACTGTATTGGCACATGCAGCATTTTCATCACAATTATGGGTGTTGTCG20.1670843776106934No Hit
AGAGCTCTTGTTTCCGGGGTAAGTCTGTCTTAATTCGAAAGTTACAGACG20.1670843776106934No Hit
GTGATATTTAACCTTATAACTCAGTCAAAAGGTCAATATTTTTTGGCAAC20.1670843776106934No Hit
CACAAGGGAACTCACCATTAATCACATTCATGTATTTATATAAAAAACTT20.1670843776106934No Hit
AAAATTGGAAATATAAGGTGTCCAACTACGGCATGTGCAGACGATGTTAC20.1670843776106934No Hit
TCCACCCTAGGCGTATAATGTATTACTAAAAAAAACCCCCTTACGATTTG20.1670843776106934No Hit
TGGAACTCAGTCAAGTCGACGTTGAGGGCGCCATCGAATCTGAGAGAGGC20.1670843776106934No Hit
TGGGTCCACTTCCTGGCATAGGTCCACTTCCTGGCATGGGTCCACTTCCT20.1670843776106934No Hit
CCACATGTTTAAAAAAAATATTTCAGATATATGGTAACACTTGAAATAAC20.1670843776106934No Hit
CTTTCTCATCTTCTGCAGGATTACAGATTGTTCTTCCCTCTTCCTCTTGG20.1670843776106934No Hit
CAACAGCTCTGTTGGCCGATGGGATGACCTTCTTGGAACCTGATGGCAGT20.1670843776106934No Hit
GATGAACCAAATGATTGGATGAGAATTTTAAAAAGTGTCTATGATTTGTG20.1670843776106934No Hit
CAGACCGTAACCCTTCTGTAACAACTCATCCAATAGCAGTACAATCAAAC20.1670843776106934No Hit
CGAAATGATTATGGGCCTCCGCAAAGTGATTCATGTAAGTCCGAACTAAC20.1670843776106934No Hit
AAGTCATTTCTAAGCTTCTGTTGTTATAACATTGACAGTACTTGACAAAC20.1670843776106934No Hit
AGTCCACAGTCACCACCATCGTTAAAGTGGTTATACTTTCGTTTCCAGCC20.1670843776106934No Hit
CTTTGAGACTCTAATCCACTGTGTTATTCAATATTTGATACACAATTGTT20.1670843776106934No Hit
CAATTTTATATCATTTTTTTAATTATGACCAATATGTCAAGGCCATCACA20.1670843776106934No Hit
TTTCCCTGACAACTACTAAGTAAATAACCCCAAAAATAGTAAGCACAAGA20.1670843776106934No Hit
CATTGTTACGCTGCTCCATGATTTGACCGCACCTGCCGTCCCCGTACTGT20.1670843776106934No Hit
TTTCACCTGAGTTGGTCCAAATAGCCATAAACATGCAACTTTGTATGCGT20.1670843776106934No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG20.1670843776106934TruSeq Adapter, Index 15 (97% over 39bp)
GACACAGTCTCAAATGTAAGTATTTGTAATGAGGGCCCCTGCATGGCTCA20.1670843776106934No Hit
CAATTCTCGCTTTATGCATGTTTCAGTGAATTACAATTTTAATGAAGCTG20.1670843776106934No Hit
CTTTAATCCGACTTAGTCAGTTTAACAACTTGTTCAAAAATGAATGATAA20.1670843776106934No Hit
GCTTTACAGGAGTCAAATGTCCTCTTATTTCAAATAAACTAGTACCTTAC20.1670843776106934No Hit
GTCGACACCGACCTCTTCGTAATCCTTCTCGAGGGCAGCCAAATCTTCAC20.1670843776106934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)