FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-5_S38_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-5_S38_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341
Sequences flagged as poor quality0
Sequence length71-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC231.715137956748695No Hit
CGGTAAGGTCCACAGCAGAAGGGTCCCACTCACAGACAACGCCATTATTG130.9694258016405668No Hit
TGAAGATTATCTTAGATTGAGTGCATTGAGTACGTGAATTATAGTTTCTT130.9694258016405668No Hit
GGTTGGATTACTATATCCATTCTTGGACCAACGACTGGAGGAGCCATTCA100.7457121551081283No Hit
ATTTTAATCATTTGCAAAAGTAACGCAAAATGAAATTAAGGGTCTCTATA100.7457121551081283No Hit
GTTATTTGTGTCCAGCACAGAAAGCTTGGTGATCACTGTATAGTATGTTA90.6711409395973155No Hit
TAACAAAAAAGTTATTGTACAGAATACAAACATGAAATGTGTACATTTTA80.5965697240865026No Hit
GTTTGTTCAAGCCATCTTGATTCATCAAAAAACATGGCTGCCAGGTATCG70.5219985085756897No Hit
AGCACTTAAACCAGACGGACTACTTAGTATAGAGGAATTGGGACCAGATT70.5219985085756897No Hit
TATGATACTTGTGGTCCTTCATGTCTGAAGAAAAGTTCTTATGAGGCCAT70.5219985085756897No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG70.5219985085756897Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACATCGCACTAGCACACCAAGATTGTGTAACCAATAAAATGCAAACT60.44742729306487694No Hit
TAACCTCATCTTCCAGACGAAAGAACAGGAGGCGTCACTTCAATGCTCCA60.44742729306487694No Hit
CTGAAGCAGGGACAATATGTTCCCATGGCCATCGAAGAGCAAGTAGCAAG60.44742729306487694No Hit
ATCTTAGAATAAATAGTCCGAATAACAGTGCGAAGGTCGTCAATCAGTAT60.44742729306487694No Hit
AGCGGCGCAGGCAACAATTGGGCCAAGGGTCACTACACCGAGGGTGCTGA60.44742729306487694No Hit
TTGAGGTGTCGTAATAGGAGTAGTAACGAATTGCTGGAGAGTTTGTGAAC60.44742729306487694No Hit
ATTAAGTGCTACATGTAGCAGTATAAGATATTCTTGCAATGTTCTGCATG50.37285607755406414No Hit
TCTAAGCTTACTTCACAATCGAGATAAAATAAATTTGTATAAACTAAAGG50.37285607755406414No Hit
GGACATGCTGAAAAGTTCATGAAATACCAGAACAAGAGGGGTGGCCGCGT50.37285607755406414No Hit
CCACAATCCACTACGAGAAACATGTTATTTCATATGAAACGAGTTCTGTC50.37285607755406414No Hit
CCCGTTTTAATTTCCAAGAATTATGTTATTGATCGTGTGTGTACTCTAAC50.37285607755406414No Hit
GGCTGAACCGTGTGCATCAGTTAGGTGATAATCCGAATCCACACAGGTTC50.37285607755406414No Hit
CTCGAAGTTGAGGCATCGGACTCTATTGAGAATGTGAAAGCCAAGATCCA40.2982848620432513No Hit
ATCTCCACCTCTGTCCCAGGAGGAAAAATCGCAAGTCATCAGACAACAGA40.2982848620432513No Hit
TTGAAAGACAAACATAGCACTTTTTGCAATTACTAAGGAATTCAGACCAG40.2982848620432513No Hit
ATGGATACTCCTACTTTGTCATGTGACTATTATATGGACACGCCTACTTT40.2982848620432513No Hit
ACCAACTTGAACAGATTGATCGGTCAGATTGTCTCTTCCATCACTGCCTC40.2982848620432513No Hit
ACCATGAAGGAAATTCTTGGGACAGCCCAGTCTGTAGGTTGCAGTGTTGA40.2982848620432513No Hit
GAGATACCGGAGTTCCCAATTGCACCAGAGATTGCGCTGGAATTATTGAT40.2982848620432513No Hit
GGCGGTGTTACGAATTTGCCACAAGGAAAACCCAGTATCCCAGAGGGCAT40.2982848620432513No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2982848620432513No Hit
AGAAGAGGCTCGTTGTCCCATCAGCCCTTAAAGTGCTGAGACTAAAACAG40.2982848620432513No Hit
CGTTGATCCATCATTGGAGTTTGAAAAGAGAAGAAATGTCCCAGTGAAGT30.22371364653243847No Hit
ATCTGGTGGTTTGTACGATGGAAATGATGTTGAAGATCATACTAAGGTGG30.22371364653243847No Hit
CAAAAACTGATAATATTCATATATATGTGTATATTTATATTCTCAAGGAA30.22371364653243847No Hit
AGGAACTTAAAGAACTTGCCGCCCTTGCTTACCGTGACACTACCGCTGAG30.22371364653243847No Hit
GGTGAGCGTCAGTTTAATGGTATGGTTGATGTATACAAGAAGACCATTGC20.14914243102162564No Hit
AGAGAAATACAAGTAATGTGAGTGGAATATACAGGTGCTTTGCAAAAGTA20.14914243102162564No Hit
GCCAAGCCTCGCTGTGATGTTGATTGCCATAGTAACAGCTCTGTACACAA20.14914243102162564No Hit
CTCGCTCGAAGATTGATCGACCTGCTGCAGCAGAAGAAAGCGCTCCAAGA20.14914243102162564No Hit
CAGAATTCCTCACCATGATGGCACGGAAAATGAAGGACACTGACAGTGAA20.14914243102162564No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT20.14914243102162564Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTTTGCCACTCACGCCAGAGGTTGTGGGTTTGATCCTCAACTCGACCT20.14914243102162564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)