FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-5_S38_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-5_S38_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC453.3557046979865772No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.1185682326621924No Hit
CCTGTCTCATGTACCCAGATATACCAACAAATACATGCATTACAGGAATA130.9694258016405668No Hit
GATTTTTTATATATTTTTTAATTGGGGTTTAACGTCACTTTCAACACATT100.7457121551081283No Hit
GTCCACAGTCACCACCATCGTTAAAGTGGTTATACTTTCGTTTCCAGCCA100.7457121551081283No Hit
CTATACCAACACCGAGACCAAAACCGCTCTTACTTCTGTCAAAAAGCCAC80.5965697240865026No Hit
TACTTGTAATGTATCCGATACCACTAGACATCTGGTGCAGATGTTCAAGG80.5965697240865026No Hit
GCAAAATGCAAAACATAATACTTCACCACTACAACAGCTGTCACTCATGT80.5965697240865026No Hit
TCTTGGTTCTTCGCTCAAACCGCTTGGGAGACACACAAACATACTCGCTA70.5219985085756897No Hit
GCACTTAATACCTTCATGCAGAAAACTGCCAGAATATCTTATACTGATAC70.5219985085756897No Hit
GTTAAAAGAGAGCATATCAGGAAAAAAGCATTATGAATATCTAATAAAAA60.44742729306487694No Hit
TGGAGGTAGACCTCAGAATGAATCTGAGTAAAAGCACCATCCTTCCACAA60.44742729306487694No Hit
ACTTCATCATCCTTTCTGATGGGAATAGATCTGACATTGTACTTCTGTTT60.44742729306487694No Hit
CTGGATATTCCTCGCGGATCTTGCTGATGAGGAGTGTTCCCATACCAGAT60.44742729306487694No Hit
ATCTCCATTAAATACAAAATTTTTAACGAAGGACCAGCCAAAAATAATAA60.44742729306487694No Hit
CTCCTGACCACCATGCCTTTTTGACGAATCACTACGGTTTGAACAAACTT60.44742729306487694No Hit
CCAATATATATTTATTTCATTTGATAAATATACTACCAAAACAAACAATC50.37285607755406414No Hit
AACGTAAATAGCTAAATTATTCACTTTTTTTTATTTTACTTGCATTAATT50.37285607755406414No Hit
GAAAGCATAGCAATGGGAAATGGTAAATATGGCTGCTTAAGACAAGTGGC50.37285607755406414No Hit
CTATATAAACTAACTGCATTTTATTTTCCAATGAAAAACATTTGCAGCTA40.2982848620432513No Hit
TCATGCTTCTTACCAGTGTGGTTAGGGATGAAGTCCATTACCATTTTCAT40.2982848620432513No Hit
CTAAGACGAAGTACAAGATGGAGTGTGGATTCCTTCTGGATATTGTAGTC40.2982848620432513No Hit
CTGGCTCGAAGCAAGCGTTGGTGATCTCGGCAACGGAGAGCTGCTCGTGG40.2982848620432513No Hit
CCAGAGCAGACTTCATGGCATCAACACCACAACCCCATTCATCCCGATCA40.2982848620432513No Hit
AATGAATACAAGAAGGACTCATTTAATTTTTGTATTGTAGTAAGTTAGTC40.2982848620432513No Hit
ATTTCTTCCAATGTTTAGTCATTTTAATTCATTGAAAATGTTATGCCACA40.2982848620432513No Hit
TTACTCATCTGGAACATCATATTGATCAGCGTCAATCCCATCAATCACGT40.2982848620432513No Hit
CTCATCCTCGGACAGAGGTGGAGAGGGCGGTCTTCCCAGTAACTTCTGAA40.2982848620432513No Hit
CCTGTCTTTATCTATATTACAGTGCTCAACTTGGTATGCTAAAATCTTCC40.2982848620432513No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.2982848620432513No Hit
CTTAAATTTATCATCAATTAATTAATTTGTCAATTATAAAATTAATTTTC30.22371364653243847No Hit
AGCAATTCTTAATAATTCAAGGGCCACAACTCTACATTTATCATCAAATT30.22371364653243847No Hit
AATCTGTTCATTATAAATTGATTTTGTCTCTTTGTTTTAATTTCTCTAAT30.22371364653243847No Hit
ACGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.22371364653243847No Hit
GTCCCCAGTCACCACCATCGTTAAAGTGGTTATACTTTCGTTTCCAGCCA30.22371364653243847No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.22371364653243847No Hit
CCTTGGCGTCGTATTCTTGTTGAATATCCCGGATGGCCTGACCAAGCTCA30.22371364653243847No Hit
AAATACCTCAACAATTTAAAACACAAACCGCTGGGAATAGTATTATTTTG30.22371364653243847No Hit
AGATTACTTGACTTTAGACTCCTTTGCTAAAGACGATTTTACATAACATT20.14914243102162564No Hit
TGAACAACAGAGCCAACCTCCATGACAAGAACATGATTATAAGGTTATCA20.14914243102162564No Hit
TGCGTGCCTATTGGATATTGGGAAAAAAAAAAAACTTTCAATATATCATG20.14914243102162564No Hit
TTCTAAAAAAAATAAAACTGTCACTGAATAAACATGGAATGATTTATACA20.14914243102162564No Hit
ATAACATTCTGGTATTTCCAGCCAATTTCGTGTGAAATACGATTCAAGTC20.14914243102162564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)