FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-4_S30_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-4_S30_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055
Sequences flagged as poor quality0
Sequence length74-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG171.6113744075829384Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT70.6635071090047393Illumina Single End PCR Primer 1 (100% over 50bp)
ACTGCGTGAATGAAATTAGTAACAATACATATTTACTAACGGTAAAGTTA60.5687203791469194No Hit
TCTGGATCGGTGGCTCCATCTTGGCTTCTCTGTCCACCTTCCAACAGATG50.47393364928909953No Hit
GTAATTGGGCATCAGTGATATTGAAGGTACGATTATGTTTATAGGGCTGA40.37914691943127965No Hit
GTCGTGAGGCTGAAAGTGATATACAGGCATCCATGACAATATTTGAAGAA40.37914691943127965No Hit
GCACAATCCAGGAAAGGGTATTTCCCAATCAGCTCTCCCATACAGAAGAT40.37914691943127965No Hit
AAGAAACCCACAAGAATTGTCAGAAATGGTTGGTGGCTCTGGCACACAGA40.37914691943127965No Hit
GTGAAAATGATCATATTGCACGTGACTGTCCAAACAGTAAAAAAGATGTT30.2843601895734597No Hit
GCGAAGGAGGCTTAATGGACCAGGCATTTGACTATGTCATAAAAAACAAG30.2843601895734597No Hit
GCAGACGAGATTTCCTGACAGGACAGAGCCTGTGCAATTGAAGCCTTGGT30.2843601895734597No Hit
TCTGAGCTTACTTCACAATCAAGCTAAAACAAATAAGTATAAACCCAGCT30.2843601895734597No Hit
GTTTGGTAGGGGAATAAAATTCAAATATTTTAAAACTACACATGTTAACG30.2843601895734597No Hit
GTTTTGAGAAGTGTGATTGTTGTACCCATACATGAGAGTTTATTTATTTG30.2843601895734597No Hit
TTTCTTTGTACGCTTTGGTAATGGTTATGGAAAGCATCATCAATGTAAAA30.2843601895734597No Hit
GACAGATAGAGACAGAGGACAAGATAACAGAGGAGCTGGTCGCCATCCTG30.2843601895734597No Hit
GTCATAATGACGTCGAAAAGGCGTAACAATGGTCGTAGCAAGAAGGGGCG30.2843601895734597No Hit
TTGCAGTTTAGTGTACTAAACTACAGATCTGATGGGTCATAATAGAAAGG30.2843601895734597No Hit
GTCTTAGGTGTACTAGTATTTATGATTTCTTTTACCATTATTTGAGTGCG30.2843601895734597No Hit
CAGGAGAACTAACATTCTGATTGAAGCCAGACATTTAATAACTATTGTTT30.2843601895734597No Hit
GTTTGAAGCTGGTATCTCGAAGAACGGTCAAACCAGAGAACACGCTCTCT30.2843601895734597No Hit
CTCTATCTAATAAGAACTTGATATAAGTTGATGTATGTTTTTATAAGCAA30.2843601895734597No Hit
GGCAACAAGGGGTGCGAAGGAGGCTTAATGGACCAGGCATTTGACTATGT30.2843601895734597No Hit
CGTGTCCTGCCTTCATGGGATGGCACCCTAATTAAAGATGAAACAAGACC20.18957345971563982No Hit
CGCACATGTAGTATGACAGTGACCATGTGCGGGTTGCTGGCCCTCAGTGC20.18957345971563982No Hit
GACGTAGCTTAACGTTCATTTAGATTTTAACATATGATTCTCGTTTTTCC20.18957345971563982No Hit
CAGAGGATTTGGAAAAATTCTTAAAAGAGGCTCGTAAAGATCCCTCGCGT20.18957345971563982No Hit
TTTTAATACCTCATTTTTTGATCCGTCGGGCGGTGGGGACCCAATTCTTT20.18957345971563982No Hit
GGTACATTTTTGGGATGTCATACCTCACTTGTCGTGCGAGGGTTACGTTT20.18957345971563982No Hit
GCGGACATTTATGGCCGCCGAAGAGTGATAGTGGTGTTGAAATCCACTAT20.18957345971563982No Hit
GTTCATCATGCAGTTGTTCATTCGTGGAACGCAGACCCATGTGGTCAATG20.18957345971563982No Hit
ATTTAGTCGTGTGTGTTTTCTTCTATCTCTACAATGGCAGCTCCAACCAT20.18957345971563982No Hit
AATGATCATAAAACGAAACGGGTTGTTAATTATACTAATTATTTACAGCG20.18957345971563982No Hit
TTTCAAGCAGAGCAGTCGAAACCCACACGTGATATTGAACGAAAACCTAG20.18957345971563982No Hit
CAACCAAATGTGGGTTTTTTAATCAGGATCTAGGCTGTGCTGATAGAAGT20.18957345971563982No Hit
AGGCATTCGAGTATATTAAGTCTTCAGGGGGTTTAGACAGTGAAGCTGAT20.18957345971563982No Hit
GGTTGGGGGAGGGGGGGGGGCAATTTTGTATTAAATTAATATTTTTCTAA20.18957345971563982No Hit
TTGACATCTCGGGCAGAACAGATGTTGGCGTTACCGATAGAACTGAACTC20.18957345971563982No Hit
TAAACACTCCACTACAAGTCTCATTAATCATAAATGTAAAGTATTGAATG20.18957345971563982No Hit
CGAAATTTTATAAAAATGGTTTAAAGTTATATGAACGAATTTTGGAAAAT20.18957345971563982No Hit
GTTCAAATGGTGTCCCTAGAGTTAAAAATGACCCCGTCCCGATGTTTCTA20.18957345971563982No Hit
CTTGGAGGACCTATGGGTATGAGTTTACCCCCACCCGGTTTTTTCCCTAA20.18957345971563982No Hit
AAAGACTTAAGGAATACAAGTCCAAACTTATCCTCTTCCCTAAGAAACAG20.18957345971563982No Hit
GGTAGGCTCATATTGGCCACAGCATAATCTCATACATTTAAGTTATTTTG20.18957345971563982No Hit
AAATCATATGGTCGGATGTATACTGTCACTTATTATTCACTTCATTTGTG20.18957345971563982No Hit
GTTTTTATCAATTCTGTACCTGTGGAAGAACGCTTTAAAGGAACACAGCA20.18957345971563982No Hit
ATTTCGTCGTACAATTAATCATTAGTTTCCAAAATTTTATATGAGATGAA20.18957345971563982No Hit
CGAGCCTGTGCCCAGCATATTCATCATCATCCAGAGAAATCCTGTTTTCC20.18957345971563982No Hit
TTAGTATTGAAGTTGTACAAATTTAATTTTGATGGATCCATGTATACCTC20.18957345971563982No Hit
GACCAACATCTAGACTCGGAGGTATTTCGGGAATGTCTGAAAGATGGGGT20.18957345971563982No Hit
CGGGGCTACTCTCAATGACATCATGGCCAAGAGAAATCAGAAGCCTGAAG20.18957345971563982No Hit
GCTGGTTCCATAACAAATGCATGTATTTTTCCAGACTAAACATGTTCTCA20.18957345971563982No Hit
TTTACCTCACTTAGGCAGTAGGTTTGAGCATAGTTTTGGCCAAAGAGTTC20.18957345971563982No Hit
TAGGCTTCGTAGTATGGGGTCATCACATATTTACGGTTGGCATAAATATT20.18957345971563982No Hit
GTGAAAGCCGGAACGAAGCCGGGTGAAACACCAAAGTAACAGTATTTTAT20.18957345971563982No Hit
GTTTTGTTTTAGAAGACATGCGTCTGCTTACTAGAGTTGTCTGTCAACAC20.18957345971563982No Hit
TGATGGTAAACAGTATGCAAAGGGCGCCACATGGAAAGATGGCTGCTCCC20.18957345971563982No Hit
TTTTATATCGTGTTCGTTTTTTATTAAGACCAACTGTGTAGCATTATTCA20.18957345971563982No Hit
TAAATTTCTACATATGTTACACAATTACAGTGGAACAAGAGAAAATATAA20.18957345971563982No Hit
CCTTATTTTATATAGAACAAAGAACACAATTCAACTGTGGAGTGCGACTA20.18957345971563982No Hit
GGCTTTATTAGTATTAGTTTTCATTATAAGGATAGTCGGGTATTTTTTAG20.18957345971563982No Hit
TTTTAGTTAATATTTAAATAGCAGGATTTGCTAAACTCTTCACCGTGGAG20.18957345971563982No Hit
CTCTGTTGCTGAGTAGTTTTGATTTTTGAATGCCCCCGGTTGGCATTTTC20.18957345971563982No Hit
CTTGGTACCATACCCACGTATCCATTTCCCTCTGGCCACATACGCCCCAG20.18957345971563982No Hit
GGACAATGTTTATCTGGGCATTTTCTAACAAAGGGAATGACAGCATGGGA20.18957345971563982No Hit
CTCCATTCGCAAAGTTCTTATTCGAAGCTGAAATCGGAGCGGCAGGAGTA20.18957345971563982No Hit
ACAATACCAAATGTCCAGAATGGAAGTTACCAGAGGAGTGTACATTGACA20.18957345971563982No Hit
GCAAGCTGGCCAGTGTGGCAACCAAATCGGAGCCAAATTCTGGGAAGTTA20.18957345971563982No Hit
GTTTTTCTTTCAAATGTTCAGAGAATTCAGCAAATGAGGTACTATAGTCG20.18957345971563982No Hit
GGACAGCTATGTAGGAGATGAAGCCCAGAGCAAGAGAGGTATTCTTACCC20.18957345971563982No Hit
CTTCAAATCAGCAACAACTTGGATTAGAGTTTGACATAAAAACATTGGAT20.18957345971563982No Hit
CTGGTAGTAAATGTGGCGACGTACTGAGGGCACGTCAACCAGTACCTAGG20.18957345971563982No Hit
TCTACATCTCGTATTCTGGATCCCAATGTTGTAGGAGATGAACACTACTC20.18957345971563982No Hit
GCACATATGGTTGTTCACCAATATTTTAAGTTAAACAACACTGCTCATAG20.18957345971563982No Hit
TGATTTTAATGCTGACAAAATTAAAATTTCTATGTCAACAGGCTTTATTG20.18957345971563982No Hit
CACACCTACACCCTCGGCATGAACGAGTACGGAGACATGACCAGCGAGGA20.18957345971563982No Hit
TATAAACAGACATCGGGTAAGCGATTTTTTAGGTATTGAACTAAATTAAT20.18957345971563982No Hit
AACGCAACCGTAGTATTAGAGTTCAGTCCAGTAAATAACAATGACGAGGG20.18957345971563982No Hit
GAACAGAGTACCCCCATGTCCCCTATCGTTTTCATTCTCAGTCCTGGCTC20.18957345971563982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.18957345971563982No Hit
CGGCCATGTTCAAAAGTAGTTGTCAAGTTTTTGACTGTGATGATGAAGCA20.18957345971563982No Hit
GGCATTAAAAGCTACGGTTGCACATTTGAAAGAACATCCACATCAAAATG20.18957345971563982No Hit
GTTTGGGTAGGCTTGGAGCTTAATTTTTTTGGGTTTATTCCTTTATTACT20.18957345971563982No Hit
GTTAATTTGTTGTTTATGTACATGTAGATTGAACCTGGAATCTTCCTTCC20.18957345971563982No Hit
GGACGCTACTCGTTTGTGTTCTGAGACTGGGAAATGGATTGGAATGCAGC20.18957345971563982No Hit
TCCATTTCCAGTTATATCCTTCTATGCATGTACTTGTGTAATTGTGTGTT20.18957345971563982No Hit
TCTATACCCCGATGCAAAACAGACGAAGCAAGCATGTTGATATTTGGGGT20.18957345971563982No Hit
GGCTAGTCAACTCTTTTTATCTAGAAATAATAAAAAAAAACCATCAAATA20.18957345971563982No Hit
TGGAGCTAGGCCTTGCTGTTTAAATTCATTATTGAGGATCTTTTTAATAG20.18957345971563982No Hit
GGCATGTCCAAAGGACATAAAACTACCAAAATAGAAGTCGTTTCCAAAAA20.18957345971563982No Hit
CTGGATCACATCCACCCGTGACGGCTATCGATGACATATCAGCAGACGAC20.18957345971563982No Hit
CAGTAACATATCCTTATGTTCATAATCTGCAGTAACAAATCCGTATGTTC20.18957345971563982No Hit
TTTGAATACAAAATTTTGAGTTTCATTTTGCTGATTGGCAGAGTTTGGAA20.18957345971563982No Hit
ATGAATGCTCACATGTTCACGCAAAATCTCACAAAAAATCGTTTTCAAAT20.18957345971563982No Hit
CTTTTGAGTTTTAGTAAGCAATATTTCAAATGGTCTGTTAGACCTAACAT20.18957345971563982No Hit
CCCACTCTCCCCATACATGATTATAGAGTTGTTCATCTTTTCAAAGAAAG20.18957345971563982No Hit
CAGCCATATTTGCTATTTCCTATTTCTATGCTTTCAGAGCAAATTATCAT20.18957345971563982No Hit
GGTTAATGTCATCCATGTAGGCTATGCAATTTAGAATAACAAAATTTAGG20.18957345971563982No Hit
TTAAGGATTAAGACATCTGAATGCATTGATGAATGCATTTAATGCGTTCC20.18957345971563982No Hit
TAATAGTTTAGGAAGTATATGCTTAAAAACAACAGGACATTTATTCTAGA20.18957345971563982No Hit
TGGAGTTCACTGAGGCCGAGTCCAACATGAACGACTTGGTATCTGAATAC20.18957345971563982No Hit
GTCGGAACTTTCCCATTCAACTCATTTTTATCCGGATTCATCTCCTGTGT20.18957345971563982No Hit
CGGAATACCAGACGAAAATTCAACAGGAATTAGATCATGTGATCGGCCGA20.18957345971563982No Hit
GTTTAAGAGGCTTGTAAGACAATTCATGAATTGTGTGAAACACACATTTT20.18957345971563982No Hit
TGACACTCTTTGCCCTTCTTCATGTTTTATAGATGATGCGGCAGCAGTTA20.18957345971563982No Hit
TGTATATTTTTATTGTTGATGTTGTTTGAAGTCATTCCCTAAATGACAGC20.18957345971563982No Hit
CTTAATATTCATTCTTTGTTTCCTTGAAGAGCAGTTAGGGTGTTTTAATG20.18957345971563982No Hit
GTTATAGGTGACTACCAGTCCTTATGTTAAAGGGGACTATGTTTTAAAGG20.18957345971563982No Hit
TCCGTAACTTGGAGAGGAAATTACGAGAGGATGAGAGGCGGAGGCAGCGA20.18957345971563982No Hit
CAAGTGTATCATCAACAAATAACAAGGCGATTGCCAAGGTAGCCACACCA20.18957345971563982No Hit
GAAATTCAATAAAATTGTGGCATGGATGAAATAAGCATGGCCTAAATTCA20.18957345971563982No Hit
GTTTGGACATGATTTTAATTAAAACGCCGTTGACTCGCTGTTAAAATGCA20.18957345971563982No Hit
AAACTGTTAGCTTCGATACAGTTGTGAAAATTGAGGTTTACATGAATACA20.18957345971563982No Hit
GGAAATAATACATTTTTTATGTGATATTTTTGCATGCTATTTTTCCTATC20.18957345971563982No Hit
GGATACTGACACTTAGAAATTACTTTTTTTAATATGGTTATATGATAAAT20.18957345971563982No Hit
CATCGGTGGTTGGCCCAGGATGCTGCCCGAATGAAAAACCGACGAATAAA20.18957345971563982No Hit
GCAAAACGTATCTACAGAAAGGTTATCGCAGCTTGTAGAGCTTCTACACG20.18957345971563982No Hit
GCCATATATAACTTGAAAAATATAACCAAATTTATCATAAGCAGTTTTTA20.18957345971563982No Hit
GTACCGTACAGTTATGAATGCACAATACTACCCTGTCACCTTCAGAAATC20.18957345971563982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)