FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-4_S30_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-4_S30_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055
Sequences flagged as poor quality0
Sequence length74-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT171.6113744075829384TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG70.6635071090047393TruSeq Adapter, Index 15 (97% over 39bp)
CTTTCTTTTATTTCAAAAGAATACCAACATTTTACGACAAGTGACAAAAC60.5687203791469194No Hit
CTATCAGCCCTATAAACATAATCGTACCTTCAATATCACTGATGTTGGTA40.37914691943127965No Hit
CAGAGATGATGCCTTTATGTAACATGTAGTTCAAGTGAGTGTACACACCG40.37914691943127965No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.37914691943127965No Hit
TGACTCTGCCTGTTGCCGACGCAACCTCTCCTCAATTTCTTCTGGTTCCA40.37914691943127965No Hit
TTTTCTAATTGTTTCAAGTTTGATTCAAATGATTTACACCTTGCGCAAGA40.37914691943127965No Hit
GTTTTTCATCAGCTAGATGTACAATAGCCGGACCTGCTTCGCCGTAGATT30.2843601895734597No Hit
GCCGCTTTAAATTCACACCTGCCGTTCTTTGGTTTGTATGGGTAGGATTC30.2843601895734597No Hit
CTTCTGCTCAGTGCAGTCACGTGACAGATGGCCTGGTTCTCCGCAGTTGT30.2843601895734597No Hit
CTTTATACCCTTCCCTTTGGTTTCAACTCCCAAAAACTTCAGTGCATTGT30.2843601895734597No Hit
GTCTGATTGCCTCTGTAACTGTCACTGTTTTACCAGTTCCAGGTGGACCA30.2843601895734597No Hit
TTTAACAATATGATTTTTTTTATTGAAGGTTAAAAATCAAAATTGGCAAT30.2843601895734597No Hit
GCAGCTAGGTTTATACTTATTAAGATTACCTGTGCAGCTGGGTTTATACT30.2843601895734597No Hit
GTACTAGTCAATAACAACTTTCTACATCTCCTTCTTGTAGAAAGATATTT30.2843601895734597No Hit
TTCAATTTGACCTCTAGCTCGGGCGTAATTGTATTTTAAGAAAAATAGAA30.2843601895734597No Hit
CTTGATCAAGTAATACAAATCCTCTGGGATCTCTGATGCAAGACCTTTAC30.2843601895734597No Hit
GCCCGCCCCCATACAGACAACCAAACAGACAAGTGATAAAGGTAAGAACA30.2843601895734597No Hit
CCGATATCTGCCGCTTTAAATTCACACCTGCCGTTCTTTGGTTTGTATGG30.2843601895734597No Hit
GGGCGGATGGCATCTAGAGCTTCGAGAAGAGTTTTTCCACTAGCATTACC20.18957345971563982No Hit
CTTAAGACAGGTTTGACTGTAACAGAAAATGTTACAAGATCACATTAATC20.18957345971563982No Hit
TAAAGTTATAAGTTATGCTATTCCTCAATTTTTTTTATTGGACAGTCATA20.18957345971563982No Hit
GGTTACATAAGATAAGTACTTATTTTACCATTGTAACCACACACAATTAA20.18957345971563982No Hit
ATCGTCATCGCCATGGTGATCATGATCGTGATCGTCATGGGAATCATGAT20.18957345971563982No Hit
TTAAAAAATAAACATAAATTCGTTATTACAACATAAAATATCACTCTAAA20.18957345971563982No Hit
TGCAAATCACAAAATATTAAAAAAGCATTGCATGAAGACAGCGGCAAGCC20.18957345971563982No Hit
TTTTTTTTACATAGATCAACTCTTTTTACTCCATTATGGTAATTTGTACA20.18957345971563982No Hit
ATAAGGACTGGTAGTCACCTATAACATAAGGATTGGTAGCCCCCTATAAC20.18957345971563982No Hit
CTTTTAATCAAACAAATAATTCAAGTAGTATATGTTTACAGAGATGACAT20.18957345971563982No Hit
GTGCTCCGTGACATGTGCGTACCAAGGTTTACTCATTAACAAAAACAAAA20.18957345971563982No Hit
TGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.18957345971563982TruSeq Adapter, Index 5 (100% over 44bp)
TCTGTACATCTCCATTAAATACAAAATTTTTAACGAAGGACCAGCCAAAA20.18957345971563982No Hit
CACGTCTGATTAATTTTTGCAGTGATGTCATTCTACCCAAAGCCCGAATA20.18957345971563982No Hit
ATTTGTCAAGAAAGAATGATGGCAGTAATGGGTTATTGACAGAGGCACTG20.18957345971563982No Hit
CACTCTCGTTTCTTGAGTCCATTTGAAATTTCTGTGGATAAACATTTTCT20.18957345971563982No Hit
TGTTTGTACAGAGTTCACGGACATGATCACTTTTTCTTGGCAGATGGAAT20.18957345971563982No Hit
CTCAAATCGTCATAAAGAGGGTATGTCATTATAAAGTGATATTCTGTTTC20.18957345971563982No Hit
AAACTACAAGTTTTTTAACCTCAACTCGAAAATTCAGTTTTCGGAACTAT20.18957345971563982No Hit
ACGGGCAATAGCTGTCTCCAGAAGTTGCAAAGCAACCTTTTCTTGACCTT20.18957345971563982No Hit
GTAAAAAAATGTAGAAATTTATGTTATATTTTCTCCTGTTCCACTGTAAT20.18957345971563982No Hit
GTAAGCCCATAACCTTCATTCTGTCAATGATACTGTTTGTACCAAGAATG20.18957345971563982No Hit
GTCCTGTCATTCCCTTTGTAAAAATATGCCAAGATAAACAATGGTCCATG20.18957345971563982No Hit
TTTGACTTTAATTTTCATTTCCTAAAAAACAGTTAAAATTGATTGTCATT20.18957345971563982No Hit
CGATTCCTATCCCTCTGCAGAGATCGACAGTGATTCTATTCTTAGCCGGA20.18957345971563982No Hit
CTCGCTGACTGGTTGAGATCTTTAACCAATAAAGTTCATAACCACTAAAT20.18957345971563982No Hit
ACTATGACATTCCGATGTATAAAGCATTTTAGACGACAATACTTCATTTT20.18957345971563982No Hit
AGAAACCCTAAAGCCCAGTACATTCTTGGCCTTAGTTGTACATTCCTTCC20.18957345971563982No Hit
CCGGAAAGTCTTTCAGGACAGGTAACAGTTCCTGTGCGTTTTCGTCTAAC20.18957345971563982No Hit
GTTTCGTATTATAGATAACTCAATACATATATACAAAAATTCTTACGACA20.18957345971563982No Hit
CTTCTAAGTCAAACCTACAAATGATCGACGTCAATTTATCATACTAAACC20.18957345971563982No Hit
TATTTAGCAAAACAAAATAACACCGCATTCCTTAGCCGTATTTCGCTTAT20.18957345971563982No Hit
CTACATGTTGCATTCCTGTGGTGGAATAAAGACCATAGGATCTGTGATGC20.18957345971563982No Hit
GTTTGATCCTCTACCACGGCTATGTGTGGTGTCCTTGAGCAAGACACTTT20.18957345971563982No Hit
GTAGAACCAGTACTGAGTGTTGTTTTATGAGAGGCATTGGGTGGACTGGG20.18957345971563982No Hit
CTAACAGCTGCTGCTTCCACTATGTTTCTGATCACGAATTTCTTGATGGC20.18957345971563982No Hit
TGCACGTCTTGCTATCTTTTCCTCTTCCAACGCATCTTCTAAGGCATCAA20.18957345971563982No Hit
GTGAATATTACCAAAACAAATATTTCAATATCAGAAGGATGGGAGTAATT20.18957345971563982No Hit
GTTAACAGTTCAACAAAGTCATTTCGTACCACGTTGATATTATAACTATT20.18957345971563982No Hit
TCTCTAATTAGTCTGTATCTGTTTCTTCACCTCCCCCACCGTAGATCCTA20.18957345971563982No Hit
TTTTTACCACATATCCCCATAATAACATGGGAGATAATACCAAACCCAGG20.18957345971563982No Hit
GGCCAAGTCACCTCCTGGTACAACAGTTGGTGGCTGGTAGTTGATTCCGA20.18957345971563982No Hit
AAAAAAAATTCCAGACCCTAAAACCTGAATAACAAAATATTTTAAACAAC20.18957345971563982No Hit
GCAGACTATAACTGAAATAAGCACGTGAGGTGACAATATATATGTTGATA20.18957345971563982No Hit
CTTCGAGGGCCACAGCCTCAAAGAACGGACAGTTCTCTCTGTCGCCCAGT20.18957345971563982No Hit
GTGAAACTACATTTAGTACATGTGCAGACATAGAATATAATATTAGTTAT20.18957345971563982No Hit
GAAAGATAGTAACCCCACAAAGGTTTGGCTGAATGTTGTCAAATTTTTAA20.18957345971563982No Hit
CTTTCATATTCTAACTAAATTTAAAATTTCCAACATGTTTTATGGAATCA20.18957345971563982No Hit
CAGTTTATTTCTTTAGCAGTACAATAAATTATCGCTTCCCGCCTACTCTT20.18957345971563982No Hit
GGACCATTTGGCCAAAATGGAGGCTGGTTTGGTCTCGTAATACCTGGGGC20.18957345971563982No Hit
CGCTCTGCTGCCTCTCGGTTTGCCATCGTGACAAATCCATATCCTCGACT20.18957345971563982No Hit
GGAAAACTCGGTACAAAGCCATTCCCTGGATGGACGTATTTTATCCCGTT20.18957345971563982No Hit
GTAGTGTCCCTTGGCCCAGTTGTTTCCTGCACCACTCTGTCCGAACACAA20.18957345971563982No Hit
AAAACAAAACAAGCACATCCAGATTGTTTAAGTTTTCCTTATATAAGCCT20.18957345971563982No Hit
GGCTGGTAGTTGATTCCGACCTTGAATCCAGTTGGACACCAGTCGACGAA20.18957345971563982No Hit
TAATTAATGTGCCCAACAAATGTACCATAAGTTACCCCACCAGTTGTACC20.18957345971563982No Hit
AGTATTTCTTTATCACAGTATCACTTAATCACAGTATCACTTATTCAACG20.18957345971563982No Hit
TTTGATTTTTTAAGTGACAGTTACAAAGCAGAATGTGATGTACATATGGC20.18957345971563982No Hit
AAAAATCAATGGTGGGGTTACACCTAAAACCAAAGAAATATCAACAAGTG20.18957345971563982No Hit
CGGTTCCGTAAAACGCATCGCCATTAAACAGAATTTCAGACCGTTAAAAG20.18957345971563982No Hit
CTTTCCCTGGCCAGTTTGATTGCTTCTTGTCTCTGCTTCATGTGGACGTC20.18957345971563982No Hit
GCAACTGCTTCCTGGAGGTGGTCTTCACTACCCTTGGTCACGTCAACAAA20.18957345971563982No Hit
ATCAAATTCACCTTTTAAAACTTTCTGGAATCCAGAGATAGTTTCCTTGA20.18957345971563982No Hit
CATTCACATATGTATTGGCTGTTTTCCACTTCGAAACAAATTCCTCCGTT20.18957345971563982No Hit
TGCAAATCGACAGTCTTTAAACTGCTGGCTCTCGCTCTCTAATGTCACGT20.18957345971563982No Hit
GGAAAACCAGGAGCGAGAATCGTTTGTAGGGCAAAATACTAGATGTTAGC20.18957345971563982No Hit
TCACTCCACACTTGTTTAATCTCCCTGTGAGGTTAACAACGATTTTACCA20.18957345971563982No Hit
AGTATACCTATGCTAAATATGACATTTTATTTTGAATATTTTAAACGAAG20.18957345971563982No Hit
TCCACCTGACCTTCCATCTCCATTACTAAGTGGAAGGTCCAGGTCACCTG20.18957345971563982No Hit
TTTTTATCTATTTTAAAACAAAATTTTATTTTTTAAGACATTTATAGCGT20.18957345971563982No Hit
GTGGAGCTTCTGTGAGCAAGACTGGGTGTTCCTCTGGGGCTACACGGAGC20.18957345971563982No Hit
GCGGATTATGAACATAATAAGGTGATGTTACTGCAGGTTATGAACATAAG20.18957345971563982No Hit
GAACACAGCCGAATTTATTAATGAAATTCCTTTAGAAACATATTCAAAAA20.18957345971563982No Hit
CAATAGTGACTTCATTGTTCGGTATACCCCCACAGGTAAATGTCAATACG20.18957345971563982No Hit
ATTGAAAGCTACCATGTGTCTTTGTGCAGTTGGCGAATTGGGAAATTTGG20.18957345971563982No Hit
CTGATGACACTGCAGTACAAATGTCTATATGACATTTTTGGCAACTAATG20.18957345971563982No Hit
GGCGAACTCAAGCTTGGACTTCTTACCGTAGTCGACGCTGAGACGTTCCA20.18957345971563982No Hit
AAAAACTCTGCATTCCTTTCTCATTACACAATACAAGCTAATCATTCTCC20.18957345971563982No Hit
TGAACAATGTCCATTTGCTGTTGGACCATAAAAACACAAAAACAAATATC20.18957345971563982No Hit
CCTTAGATGACTCTGCCACAGAAGACCTCATAACCTCTGAATAAATTAAA20.18957345971563982No Hit
TTTTTTATCATTGTTTGCAACCCCCAGGAGTACTCGAGCTTGCTTCTCAT20.18957345971563982No Hit
AGTGTATGTAACCTTAGATGTTTTATAATATTTTAAACACAACTATGATA20.18957345971563982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)