FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-1_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-1_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073
Sequences flagged as poor quality0
Sequence length68-151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC11610.81081081081081No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121.1183597390493942No Hit
GTTTTATCTGAATATCTGTTAAAAAAAAGTATTAATTATTAAAACAGTCT70.65237651444548No Hit
CAGTATGAAGCTAAACATATCTTATCGGGCCACTGGCTGTCAAAAATTAA60.5591798695246971No Hit
TTTTTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.5591798695246971No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG50.4659832246039142Illumina Single End PCR Primer 1 (100% over 50bp)
CACGCCTACTACCACCACGACCACACCTACAACCACGACCACGCCTACAA30.27958993476234856No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCC30.27958993476234856No Hit
AGAAATTGTACGAGAGTTCCACTGGCACATACCATAACGGATGGCAATAA30.27958993476234856No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCCCCCC20.1863932898415657No Hit
GCCTGGTTCCCTGAAATTATAGCATAGTGATTTGGTATTGAGTCGGTGTC20.1863932898415657No Hit
CAACTTCCCAGCCCACGGAGAAATGTCAGCTGCATCATGGGAACATCCTC20.1863932898415657No Hit
GGTGCCAGCTGTATTGAAGAACATTGTTATTACCTCCCTTGATGGACACG20.1863932898415657No Hit
CCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1863932898415657No Hit
CTGAGATCTCGTTCATCCACATCTGGTACTGGACACCTTGCTTCAAGGTC20.1863932898415657No Hit
CCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1863932898415657No Hit
CCATTGCAGACCTGGACTCTGTTTACGACTCACCAGAAGACATCGATCTG20.1863932898415657No Hit
CTTACATAACATTGAGAGATAATTAAAAAAAAATAATGAACATACAAAAA20.1863932898415657No Hit
CTTAACTGTTATATTATTATAAAACTATTTTATTATTATTCGTTTAACGT20.1863932898415657No Hit
CCGCCATCCAGGAGCTGTTCAAGCGTATCTCCGAGCAGTTCACCGCTATG20.1863932898415657No Hit
CAGAAAGGCACAGAGAGAACAAGCCATCCGAGCTGCTAAAGAAAAGACAA20.1863932898415657No Hit
CTTCCAAAAATAACAAGAGTAATGCATGTTTAAAGCCATTTCATTGTCAT20.1863932898415657No Hit
GTTTGTTCAGAAAACATACCGTTTTATGAAATATTCATTCCGAGGTTGGG20.1863932898415657No Hit
CCCCCCCCCCCCCCCCCTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1863932898415657No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCA20.1863932898415657No Hit
CTTGGATCAAGATGCGAGCCAAGTGGAGAAAGAAGCGTATGCGCAGGTTG20.1863932898415657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)