FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-1_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-1_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073
Sequences flagged as poor quality0
Sequence length68-151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC11510.717614165890028No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC514.753028890959925No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC201.863932898415657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161.4911463187325256No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC141.30475302889096No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.8387698042870456No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC90.8387698042870456No Hit
TTTTCATTGAAATCACATCAGTGTAAAGAATGGTAAATAGGGGAGATAAT80.7455731593662628No Hit
GCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.65237651444548No Hit
GTACACCCTGCTTCATTGGGAATCCTTGCTTGTCATTACCACCAGTAATT60.5591798695246971No Hit
TCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.3727865796831314No Hit
ACCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.27958993476234856No Hit
CCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.27958993476234856No Hit
TAAAACCTGGAGGGGGCGCAATGCCAGCCAATGATCGACAATCGTAGCAG30.27958993476234856No Hit
CCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1863932898415657No Hit
GAACCTGTCTCCATATTTTAGTCTATTAAACTGATCTCCAAGAAGACATT20.1863932898415657No Hit
CACTGCAGCTGTAGGTAGATATTCCATACCTTCACATGACTTGTGAAATA20.1863932898415657No Hit
ATTTTTTTTTACAGATTAACATTTAGTAAAATCAAATTTAACGTTAGTAA20.1863932898415657No Hit
GGCAACCATGATAAACAGATGATCACATTTCTTTATTCCATATATTGTAC20.1863932898415657No Hit
AGATAAAATAATCAAACTATACCAATAACAATACTAAACTGAGTTCAAAC20.1863932898415657No Hit
ACCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.1863932898415657No Hit
TGAATATAACATGATACTATAGTTAAAATTTCGTACAATAAATGTTTTTA20.1863932898415657No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACC20.1863932898415657No Hit
ATTTCTTTAGCAGTACAATAAATTATCGCTTCCCGCCTACTCTTGGTGCC20.1863932898415657No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCC20.1863932898415657No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCC20.1863932898415657No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG20.1863932898415657TruSeq Adapter, Index 15 (97% over 39bp)
TTACAATATACACATTATATATTTGTTAACAAATAACCCAGCTGTGGTCA20.1863932898415657No Hit
TTAATGAATTGCCATTGCTCAGTTTAACATGTTTACTGTGTTTATATAAA20.1863932898415657No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC20.1863932898415657No Hit
TTTTACTTGACCTCTTTATTGGTTGACAACAATCATCTCGGATGAACCCG20.1863932898415657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)