FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-116-7_S53_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-7_S53_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA512.972027972027972RNA PCR Primer, Index 36 (96% over 30bp)
GTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGA80.4662004662004662RNA PCR Primer, Index 36 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC80.4662004662004662TruSeq Adapter, Index 5 (100% over 50bp)
CTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAA60.34965034965034963RNA PCR Primer, Index 36 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC60.34965034965034963TruSeq Adapter, Index 5 (100% over 50bp)
TTACACACGGGTCATATATTCATATTAAAGCGGCCATCTTTACTTATAAA50.2913752913752914No Hit
CGAAACCCATCACCTCAACCCATCAAAGCAGCAACACTCTAACCACTGCA50.2913752913752914No Hit
GCAAAACCCCATTCGTTCATTAAACGAACTCACTTAACTACTGCAGCTAT50.2913752913752914No Hit
GGAGACTACTGTTCACTGTTTATGGACTAAATTGTCATTTGTAGGACATT50.2913752913752914No Hit
GTCAATATCAAATAGCCGCCTCCAACCCGAAATGATTATGGGCCTCCGCA40.2331002331002331No Hit
CTCAACCAAACTCAACAAATAATTATTAAAAGAGGCATCGACGCACAAAC40.2331002331002331No Hit
CCCGAAATGATTATGGGCCTCCGCAAAGTGATTCATGTAAGTCCGAACTA40.2331002331002331No Hit
GGACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA40.2331002331002331RNA PCR Primer, Index 36 (100% over 29bp)
TGGAACTATATTTATTAATGGACTCTCCTTATTTGAACTAAGAATATTAC40.2331002331002331No Hit
ACACTGACACGGGAAATACTGAATATGGTACACACTACAATACAGGCAGT40.2331002331002331No Hit
CCGGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCT40.2331002331002331RNA PCR Primer, Index 36 (100% over 27bp)
CTAAACTTTACTTTATTACTTTCTTAATAAAAATATATAAGCTCGACAGG40.2331002331002331No Hit
AAAAATTTGTGACACAAGTTTGTTGAAGGAAAATAAAATAAAATCAACAA40.2331002331002331No Hit
TTTACACCGGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT30.17482517482517482TruSeq Adapter, Index 13 (95% over 24bp)
AGCGAATCCAGGCATGAAGAAGTGGAGACGGGGGAAGGGAACCATGTTGA30.17482517482517482No Hit
TTTTAATTGGCTTATATCACAATATTTACATTCCAACTGAAATCAGCCTA30.17482517482517482No Hit
GCATTCTGCTGTTGCTGCTGGTATGGACCACCAAATGTTTGTCCAGTGCT30.17482517482517482No Hit
TTTTGGAATACGTTTATTTCTAAACATTAATCAAGAACAACTACAACAGT30.17482517482517482No Hit
GTGAACTGTACTTGAACCACGTGGTACTGAAGCTGTGTGTTGCGCACGAC30.17482517482517482No Hit
GCACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA30.17482517482517482RNA PCR Primer, Index 36 (96% over 30bp)
TTTACATCAGTGAAATTTATTTCACGTTTGTTGTACAACTTGGCTTAAAT30.17482517482517482No Hit
CTTTTTTTTTAATTATTTTCTCAACATTAACGCTTAGACTTCAAAAACAA30.17482517482517482No Hit
ATTTACCAGATAAATACATGTAATTATATATTTATCAGATATATACATGA30.17482517482517482No Hit
CTGGGTAGATGGCAAATTCAAGCTTGGATTTCTTTCCGTAGTCGACACTG30.17482517482517482No Hit
AGAAAAAGATGCGCCTATATACTCTTCCAACTGAAGAACTGAAAAATACC30.17482517482517482No Hit
CTTGATTCGCATCGTCTGCTCGTATGTCAGACACGTGTCTCACCGCCCCG30.17482517482517482No Hit
ACAATATGAACAACATTGAATGATCCGAACAATGACCCTCCACACACCAC30.17482517482517482No Hit
TCCATGGAATATTAACCTAAATAACTATGTGACACTAAAGTGAAAATCTT30.17482517482517482No Hit
GTTTTCCTTCATTCGGGAGACGTAGTCATTTAATGATGCCAACTCATCTC30.17482517482517482No Hit
TGATAATGTGTATAAAATAAGGATTATTTACAACCCTAAAATTGGCAAGA30.17482517482517482No Hit
CGAACGCCGCCATGACAGAAAATGGTTTACCATTGTATGAAGTTTTATTA30.17482517482517482No Hit
TATTATAGGTATCACCAGAAAAAAAATTATAACAAAGGCATGCGCAGTCA30.17482517482517482No Hit
CTACAAGCCCGCCCCCATACAGACAACCAAACAGACAAGTGATAAAGGTA30.17482517482517482No Hit
GCGCACTTTACGCGTTGGTTTAGCTTTTGGAGTTGGGTCCTCTTCGTCAG30.17482517482517482No Hit
TTATGTTCTTGTATTGTGCACTACCATGTATGTACAGGTGCATGAGGTAT30.17482517482517482No Hit
GAAAATTTTACATTATGTTTTAACAATTTATCTTAATTATGAATAATTTA30.17482517482517482No Hit
GGGCAATCACCCGAACAAACCCGAGAAAGCTTTGTGTAAGAATGCTTCAT20.11655011655011654No Hit
CGGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTT20.11655011655011654RNA PCR Primer, Index 36 (96% over 29bp)
GGATTCGTCATATTCCTGCTTGCTGATCCACATCTGTTGGAAGGTGGACA20.11655011655011654No Hit
ACGACCCACTGGCACTGTTCCAATACCTCCAATTTTGTAAACATCCTGCA20.11655011655011654No Hit
CCCTATTGCTGCGCCGTCCGCCCGAGTTACGCATGAATTGATTAGACTCT20.11655011655011654No Hit
AACAAATACCATTTAAAGTCACTGACAAACTATAGACATTTAAAGCGATT20.11655011655011654No Hit
GCATCGTCTGCTCGTATGTCAGACACGTGTCTCACCGCCCCGAAACCAGT20.11655011655011654No Hit
CTGGGATTCCAGACCTGTCTACTGAAATGAACTTTGCACCATAATATAGT20.11655011655011654No Hit
GCAAATTAGGTTTCTTTTATTTCTACACCACATATTTATCGTAACATCGG20.11655011655011654No Hit
CTAGTTTACACCGGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCC20.11655011655011654TruSeq Adapter, Index 13 (96% over 25bp)
ATACGCCGGTCACATGCACGTCCATACCAAATTCAAAAGTGTTAGTCAAG20.11655011655011654No Hit
GACAGTGTGCGGCCATCTTCCAACTGTTTACCGGCAAAGATCAATCTCTG20.11655011655011654No Hit
GCCAGATCTTCTCCATATCGTCCCAGTTTGTGACGATACCGTGCTCAATT20.11655011655011654No Hit
GGCGTCCTTAAGTCTCTGAGTCAAGTCACGGATCGTCTCTTCGTAGCTGT20.11655011655011654No Hit
CTTTCGTACAAAGATAACTAAAAATTAGAGATAGAAACCGACCTAGTTTA20.11655011655011654No Hit
CCCGAAACCAGTCATACCGGACTGACTTGCGTAAATATTTGTTTTCGCTT20.11655011655011654No Hit
TTTTATTTGTAAACATCCAACCAATAGTTTATTCTGCTTCATCTTGAACA20.11655011655011654No Hit
CGACCATTTTCTTTCTGATCAGTTTGATCTGTGCATGCTGGGCATAGCAG20.11655011655011654No Hit
TGAAAAAGAACTATAACTCTACCTTGCTTACTTTTTGAATTATCTCCCTT20.11655011655011654No Hit
TTAAATATCTTTCTCTTGTTTTCTTATTATTACTGTCATAACATACAATT20.11655011655011654No Hit
GTAACTATTCGTCCGCATCTGACGGAATTTGTTGCGGTTCTTCTTCCGTT20.11655011655011654No Hit
ATAGAAACAAGACCCGCTCTTGCACACAAGATGCGTCCATCGTCACATGA20.11655011655011654No Hit
GGCCTCTCTCAGCGGATTCGAGAGCGGAGCGGATCTCTTCGCATTCTCCC20.11655011655011654No Hit
CCGAAACCAGTCATACCGGACTGACTTGCGTAAATATTTGTTTTCGCTTG20.11655011655011654No Hit
CAGCGATACGTATAACAAATTATCAAAGATTTATTTATATTCGCTACATT20.11655011655011654No Hit
CTTGAGAAGGCAAACATTGGGGGTATCACGAAAGTTTGTATCCAAGTGTA20.11655011655011654No Hit
ATTTAACATAAGCTTCTTTAAAACAAAAGCGAATGGCCGAGCACGAAGAT20.11655011655011654No Hit
TACAAAGATAACTAAAAATTAGAGATAGAAACCGACCTAGTTTACACCGG20.11655011655011654No Hit
TCGGATTTATCCAATGCATTTTCCTTTTCGATTTTCATCGCCTGCATCTT20.11655011655011654No Hit
CGGATTTATCCAATGCATTTTCCTTTTCGATTTTCATCGCCTGCATCTTC20.11655011655011654No Hit
CGAAAGTTCAATAAAAATTTATATAACGTCATCATATTTGAGAGCGAAAC20.11655011655011654No Hit
TGCTCATCATGTGTTTCACATTGCTCTTGAATTACATCAGTTTCTCCAAG20.11655011655011654No Hit
TTAAACATTTGAATTGGGCAACCATAACTGAAGCAGGGTGATAGAGTGTTAGTCACTCAAGCTAGAGGCTGCATG20.11655011655011654No Hit
ACGGCGTCCACCTTTAGACCTAGCGAGGGTAAAGTCCAACAATGCATCAC20.11655011655011654No Hit
GTATCCCCAAGGTCCTTTGTTTCTACCTCTGGCAGATATTTGTCAGGGTG20.11655011655011654No Hit
CAAGACGCCATGCAAGGTAAACTTCCTTGAACTTGTCAAAACCGGACAAC20.11655011655011654No Hit
CGCAAATCTCAGAACACTGCCCATACACTACACCAACATGATTTCTCTTT20.11655011655011654No Hit
GCGAGGCTCTCCAACCTTGATTGGACTCTCCAACCCCTTGATTTCAACTT20.11655011655011654No Hit
TTACTATCCAACATTGTTGTTTGGGTTTCCTATGTTAGTTATGGAGCATT20.11655011655011654No Hit
TAACAAAACATATATCAGCCTCAACATTGAGACAGGAACCAGACACATCA20.11655011655011654No Hit
CACAAAACCAGATACGTAGTTGCTAACGTTGATATTGCTTCCAATGACAT20.11655011655011654No Hit
CAGCACGCCGCTATCACCCCCGTACTGTCGCTGTCCCCGCTGTGGAACAT20.11655011655011654No Hit
TAAGCATTCAACAACAATATCATTGAAAACACTTAGTTGTTCAGCTGTTT20.11655011655011654No Hit
CCCAGCAACAGTCTCTGTGATGATTGATATATGAAATCATTCGTCTCTCA20.11655011655011654No Hit
ATCCACAAGTACAATAGTTCAGATGTTGTCATTTTAATGTTTAACAAAATTCCGTTAGGCTTCATAAGGCTG20.11655011655011654No Hit
TTTTTTGATAGTGGCATGTTTTATTATTTTTTATCCTCCATACTCTTTCC20.11655011655011654No Hit
CACAATTCTTATCCTGTGATACGGCCAATCAGCAGGACAAGCCCCCAAAA20.11655011655011654No Hit
GGCAATAGTTTCCTTTCCAGAAAGGTCGGTCACATGAACAAATGTGTCAT20.11655011655011654No Hit
GGGTGTTGAAGGTCTCGAACATAATTTGGGTCATCTTTTCCCTGTTAGCC20.11655011655011654No Hit
TTTTTTTTTGATATATATATTCTTTATTTCCTCCATTAGCTAAGAGCGAC20.11655011655011654No Hit
GTCAATATCTTTGGCTTCCACACCAGTCTCGTCAACCTCTTCCTCCTCCT20.11655011655011654No Hit
CAATACCGAGAAACAACCTCCCTGTGCAGCTGTTGGTACCCTAATTTCCA20.11655011655011654No Hit
CAATTTTCTTGAAAGTTCTAGAAGTCAAATTTCAGTTTCCTGGGTGTCAT20.11655011655011654No Hit
TTTTGTCCTGTTTCAGGGAAAACTTCTAGCGGTTGCCTCACAATTAGGAT20.11655011655011654No Hit
TGTAGAAATGAAATTAATAGCCCCCAAAATAGAAGATACCCCCGCCAGAT20.11655011655011654No Hit
CTCGTCAACCTTGTAGTACTTGAGTACAGCAAGCTTGACCTTCTTCTTCT20.11655011655011654No Hit
CTAAGAGCCCCATCAATAAACAAAGATAAACAAAAAAATCCAAACTACGT20.11655011655011654No Hit
TTACACAACTCAACCCCTTGCACTCATACGGTTTTCAGAGAAAGTGCTAC20.11655011655011654No Hit
GTGGAAATCTGGGGTATTTATGATTTCAAGGAAGTGGATAAAATTCATAT20.11655011655011654No Hit
CTCTACCCACAAAAACACTAGCTCCAACAGCACAAGTAGCAACACACACG20.11655011655011654No Hit
TTAAGCTCACAATTACGTGAAGTCTATGTAGTATGTATGCAATCAATCAG20.11655011655011654No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.11655011655011654No Hit
GCTCGCTCCACGTAAAACACGAGATTTTGTACAAAGTTTACCTCATCCGT20.11655011655011654No Hit
CCACTTCCTCAGCTTCCTCAACTTGGCGTTTATATGTCTTAATCTTGCTC20.11655011655011654No Hit
ATTCCATCAAGACCATGTGGACCTGTTGAATATACGAATATTGACCGAAC20.11655011655011654No Hit
GTCACTTTTGAAAATATTAATAAATATCAATTCAATTGCAGAGACAAACA20.11655011655011654No Hit
TTCTGTTCCTTCCAAAGATCTCCATTGGCATATAACATAGACGGGTACTT20.11655011655011654No Hit
TTTAGCTTTTGGAGTTGGGTCCTCTTCGTCAGTCGGATACTCGTCGTCGT20.11655011655011654No Hit
GTGTATTTACACATGAATGCGATATGAGTAATGAACCTTTTTTTTTTATA20.11655011655011654No Hit
GCAGGGTCAGCACAGGCTGCTTCTACTTCTGAACCAACACAGTCATTCAG20.11655011655011654No Hit
TAAAAATTAGAGATAGAAACCGACCTAGTTTACACCGGTCTGAACTCCAG20.11655011655011654No Hit
CTTTTTTGTAAGCAGCAAGTGTAGAAAAGCACTTGTAAACAACAACCATA20.11655011655011654No Hit
GCGACGCAGAAAAGGCCGGAGTAGGTGTAGATAAAGCCACTAAAGTATCT20.11655011655011654No Hit
AAAATTTTGTCACTCTTATTTGCTAAAACAGCCTACAAAATAATTAAGTT20.11655011655011654No Hit
GAGCTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA20.11655011655011654RNA PCR Primer, Index 36 (100% over 29bp)
GAAATACTAATTTATAAAATGTCCTATAACATGATGAGATTTATTTAAGG20.11655011655011654No Hit
GTCAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAA20.11655011655011654RNA PCR Primer, Index 36 (96% over 29bp)
AGGCAATGTCATCACCTACACACGTAATCTTGTAACCTGGCAGTGTTGGC20.11655011655011654No Hit
CTTAATTATCTGTTTATTCATTACATTCATAAAATTAAAGCCCCAACAGC20.11655011655011654No Hit
TTTTTTTAATTTTTATGCAACATATTTTTTTCCATTTATTTATTACAAAA20.11655011655011654No Hit
TCAACCTCGAGTGTGATGGTTTTACCAGTGAGGGTCTTAACAAAGATCTG20.11655011655011654No Hit
TTTTAACTATACTACAGAAGGCAAAGGTCCTGCAGATTTAATCAACGCCC20.11655011655011654No Hit
GTCGCCGTGTGTGCACGGTCCATGATTTGCCATGCAACTTGCTTGAGATG20.11655011655011654No Hit
CCAAAGAACCTTCATTCAGTTCATGTATTAACCCTAACAAGAGAACTAGT20.11655011655011654No Hit
CGGACTAGACCCATATATCCCGCAAGTATCTAGTAAACGTTCTCACATAA20.11655011655011654No Hit
TTTCAAAATACGACCACGGGCACGTTCTGTCACTTTCAATGCACATAACT20.11655011655011654No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT20.11655011655011654TruSeq Adapter, Index 5 (100% over 50bp)
GCCCGATTCACTTGTCCTACTGATGTCATAGATGAAGACATAGATGCCTG20.11655011655011654No Hit
GACACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA20.11655011655011654RNA PCR Primer, Index 36 (96% over 30bp)
CTCGAACATGATTTGTGTCATCTTTTCTCTGTTTGCCTTGGGGTTGAGTG20.11655011655011654No Hit
TCATTTTGCATGTTATCCTGTACCATTGTTTCGACTTAATCTGACTTATT20.11655011655011654No Hit
GAAATCCTCAACAATTCCTGAATTCTTATCTCGTATGCCGTCTTCTGCTT20.11655011655011654Illumina PCR Primer Index 10 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)