FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-116-6_S45_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-6_S45_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.2165450121654502No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG120.9732360097323601Illumina Single End PCR Primer 1 (100% over 50bp)
AACAAGCATGTCCATATTATGAGAAGAATGTATTGTTGTAAATTATAGTT110.8921330089213302No Hit
TGAAAGGAAAGTATGTGTTAGCTTTTTATTTGGAGGTACTGAAATTGAAG90.7299270072992701No Hit
TTAAGACATATAAGTGACAGTTTATAATTATTATATTAAAGAGTCGGATA90.7299270072992701No Hit
GACGAACTCCCTGGAATCGGCTTGCCCGGAGATAGGGACGTTGTGCCCGT80.6488240064882401No Hit
GTACAGTTTTGAATCCATACCATTATTGTTGGTTGCTATTTTTACTTATT70.5677210056772101No Hit
TCCAAGGATCGAACCTTTCGATTCGAACAGAAAACACATGAATGATTCTC70.5677210056772101No Hit
CGGCTGTTTAAGCTTTGAAACTGGTATAAGAAGAATGGATACGGGAGGTT70.5677210056772101No Hit
GATGTGATCTGTAACCATGTAAAATGTTATTTTCTGATCCATTTCCATTG70.5677210056772101No Hit
GTTTATTTGCTGTCAAATTCTTCACTAAAACCATCCAACATACTTCAATA70.5677210056772101No Hit
GAGGTCTTCATTAGATTCTTGAGTCAATACCATTATTAAAGATAGTTTTT60.48661800486618007No Hit
TTAACCATAAAACCTGGCAAAATCAGAGAATTATGTACGAGTATGGTCTT60.48661800486618007No Hit
CTGAGACCAGGCAGTAAGGTCATGTAGATAATGCCCACCAACACCGCCAA60.48661800486618007No Hit
AATTGAAGATATTCCATTGGTTGTCAAATGAGGTTTTAACACTAATGACC60.48661800486618007No Hit
CATGTACCTGCCATTCCGTCTAAAACAGCTGGATACTAATTAGTTTTCAA60.48661800486618007No Hit
AGAACGACCCGTGGTAAGGAGGCCATTTCCCGATTGAAGGTGTTTGAGGG60.48661800486618007No Hit
TGTACGTGAAAAACCTCGATGATAATATTGATGATGAGCGCCTACGAAAA50.40551500405515No Hit
CATGTGATACGAGCTAAAACAAAGATACGCAGTCAGATTGTCCTTCCAGA50.40551500405515No Hit
TTCAACACTGCTCCAAAATTCGATGATGAAAATGATTTCCCATCGCTCCT50.40551500405515No Hit
CTTTAAAGATAAAACCAGTAAACTACACAAAACAATCGTCTTAATTATTT50.40551500405515No Hit
TGCACGTTACATTTTTTCACGTCTGCTGCTGGTTCTTTTAGACAATACAG50.40551500405515No Hit
TGAGTATGCAGCTTAAACAAATGGAACAATTTTGTCTTGCAAGCTGAAAC50.40551500405515No Hit
TTAATTTGCAAATTATGACCCATTCGTGTGCTGTTTCATCAATATTTAGT40.32441200324412006No Hit
CTTCATATCAGGGAATTTCTTCAAATCTTACACAAGCAAATACAAATCTT40.32441200324412006No Hit
ATTACTTATTTCTATTTTATTGATAAACAGTCTACCATTTTTTCACCATG40.32441200324412006No Hit
CTTGGAGAGACGTCTTCAGACACAGGTCTTCAAGCTTGGTTTGGCCAAGA40.32441200324412006No Hit
AGGCAACCAGCCCTACTATGGAGCCAGAAACCAGCCCTACTTTGGAGCCA40.32441200324412006No Hit
TGTAGTGAGACCTGAAGAAAATACTTTTAAAAAAATAATGATGCGGAAAT40.32441200324412006No Hit
AAACAGCATCCGAGATATGAGAAAAAAAAGTATAAGAAAAGTTTGGTAAA40.32441200324412006No Hit
AATAATTACTGAGTGTATGCTTCAAAATCTGGAGTGCATCTCTTAGCTAG40.32441200324412006No Hit
GTTCCTGACATCTATGCGATGCCAATGAAGAAAAATGAGGACAAAGCACA40.32441200324412006No Hit
TGTGCATAGTGTTGGCCCCTAATGCCAGAGGTTGCGTATTTGACCCCAGC40.32441200324412006No Hit
AATCACGTGATTTCTCGAAATCACATGACACAATATCGTCGGCGTCAGTC40.32441200324412006No Hit
AAAACATGACCAAACAGTGATAGCAGAAGATGGAACACAATATTTTTATA40.32441200324412006No Hit
GTCTTTCCAGCTAAATGCTGATACATCTACAACAGCTCTCTCGGCAGACA40.32441200324412006No Hit
TGTGAAGACATTGACTGGCAAGACCATCACCCTTGAGGTAGAAGCCTCTG40.32441200324412006No Hit
GGGAGACTGTATGGTTAATTGTGTGTAATATATCTAGACCCAGGGAGACT40.32441200324412006No Hit
TGTCGGTCGGTATGTAAATCGGATATCTGTGTTATTAAAACGACTTACAC40.32441200324412006No Hit
GTGGCTTCTTCCTGATCAAGATGAACAAAGGGAAAGTGTTGAGCGATCAT40.32441200324412006No Hit
TGGTGAAACACCTTTAAATTACATGTAACCATTGGATTTTACATTACTTG40.32441200324412006No Hit
GACACAAATCATGTTCGAGACCTTCAACACCCCCGCTATGTACGTCGCCA40.32441200324412006No Hit
TGGATTTGTGACCCATCTGATGAAGAGAATCCAGAGGGGACCAGTCCGTG40.32441200324412006No Hit
CAGTCATCTCCGCCGAGAAGGCCTACCACGAGCAGCTCTCCGTTGCCGAA40.32441200324412006No Hit
TTTCTTTAAATATTGTATAAAACTTACACTGTTGAATTTTATACAGAATT40.32441200324412006No Hit
TTCAGCTGATGAAGTTTAACTAATGTCTATATTCACTATCCCCGGTGCCA40.32441200324412006No Hit
GATTTAGCTTACCTTTTCTTCACTTATTGAATTTTTATAATGAGTTTGAT30.24330900243309003No Hit
GAAAGGGAGTTGCAATAAACTTTATCACTGCCGATGACAGCCGGGTTCTT30.24330900243309003No Hit
CATTGATCCACAGATGAATAATCAGCCATTGTCATTCAATGCCAACAAAG30.24330900243309003No Hit
ATTCAGAGGACAATTTTAATGATGGGTCGTTACATTGAACCAATTCCAGA30.24330900243309003No Hit
ATGGCAAGAATAAATTGAAGGTCATCAAGTGGTTTGGCAAGCGCAAGGAA30.24330900243309003No Hit
TATCGATATAGTCGGTCAATTATCGGGATCACATGATCCTTTTAATGATC30.24330900243309003No Hit
AGAAACGTGCTTCATTGAAAGAAGTGCAGGACAAGCTGAACAAACTGACG30.24330900243309003No Hit
CGTTACTGTAAGGACTATCTCTCTCTCTGTCTGTCGGATTTTATTAATAA30.24330900243309003No Hit
GGAAGCGATCAAGTCGTTGTGGGCGGACAGTGGTATACAGGAGTGCTACG30.24330900243309003No Hit
GTTTTCTTGAGTTATTTTGCCATGTGGATGCAACTGTATGCCTTAAATGA30.24330900243309003No Hit
GCCACTCGTGCTGTGGAGATGGTGGCTGTAACTTTGGAGGATGTCTAGAA30.24330900243309003No Hit
GCTCAGTCCTTCACACAGGATTTCGAAAATGGCGACATCATAATATCGTC30.24330900243309003No Hit
GTAAAATAGAAGTTAAGTCTACCTCATTTACTAAAGTAAAAGACATAGCT30.24330900243309003No Hit
TCGCATTTCATTTTGATAATTTTTTGCCAATTAAATGAAATGCTGTTTAA30.24330900243309003No Hit
TGGTAAACAAAGAGACCAAGAAACTTCCAGAATTTTTTTAAGTTTTGAAA30.24330900243309003No Hit
CGCACTGGCTTGTATAGGCAATTGTTCCACCCCGAGCAGCTCATCACCGG30.24330900243309003No Hit
GTTTTTAAACTTTGATATATTTTAAACCCCTGCTAGTGTACAATACATAT30.24330900243309003No Hit
GGTACTGGTTCTGGTTCATCAACTGGTTCGGGTACCAGCTACAGGGAATC30.24330900243309003No Hit
CAGCTCCCCAGATCGCCACCGCCGTCGTCGAGCCATACAACTCCATCCTG30.24330900243309003No Hit
CGAGTTTGAAAATTTTAAAATTTGAATTTGACTCGGTTTGAGTCGTATGA30.24330900243309003No Hit
CTTACCTTTATGAAAGAATTCCAATAGTACAGCCACTAGTGCAGATATAT30.24330900243309003No Hit
TTTTTTTGCAAGATTTTGATTTTGCATACCATTCATGGTTAGAAATCATG30.24330900243309003No Hit
ACGCATTCCAAACTGGTGGAAAGTTGTACCTGATCCTAGAGTATCTCCCT30.24330900243309003No Hit
CAGGGATTCCTTACCAGATGAATATCGCCCTACTTGGTTTCATTGTTGGG30.24330900243309003No Hit
CCATGCTGTACGTGCAAGATGGGACCAGCCTCTGACGAAACAGCGGTTGT20.16220600162206003No Hit
GGATGATCGAGACACAAACGGGAAGCAAATTTTCGGACACGGTAACAATC20.16220600162206003No Hit
CAATCAGCGTGCCCCTAACAACGCTATGTGATGGATCCAATCGGGGAGAG20.16220600162206003No Hit
GAATGTGAAAGCCAAGATCCAGGACAAGGAAGGAATTCCCCCAGATCAGC20.16220600162206003No Hit
CAATCGGTTGAATGAAGAAAGTATTCCTCGAACAAATACATTGAATCTTC20.16220600162206003No Hit
AATTAGTCATGTAAGATATGAATTTTACAATGTACTGGTACAATTTCATT20.16220600162206003No Hit
CTCAAACGACCGAATCCAAAATCAATATTAATGCAGAAATGCCGAACCAA20.16220600162206003No Hit
TGCGTCTCTGGAGCCCCAAAATAGATTTTACCTTTTTAAATCGGTGTATT20.16220600162206003No Hit
TTTTGAATTATGACTATGGATGTTGTTAACACTCTGGTATTGTGATATCA20.16220600162206003No Hit
GAAGGTTCCTGCACGGGGTATGGCTGCTTTTACCGATCACCTTCAATACA20.16220600162206003No Hit
CTTTCAGTGTCAACACCTTGTTACATAGAAATGATAATATCTTAGATGAT20.16220600162206003No Hit
GTTTGTAGTGAAAGTAAGATAATTTTTCTTTAATCTATAGTGGTATTTAA20.16220600162206003No Hit
CGACTACGGAAAGAAGTCCAAGCTTGAGTTCGCCATCTACCCAGCTCCCC20.16220600162206003No Hit
TCACTGCTGGATATTACTTGTACTATATACATATTTATTTTCGATACCCT20.16220600162206003No Hit
GTATCATATATCAATCCTCCTTACACATTTCTGGTAAGCTGTAAACAGTT20.16220600162206003No Hit
GTGATCTTGGTCATCAAGATGCTTTCATTATTACCTACAAAAAATATGCA20.16220600162206003No Hit
CCGGTATCAATGTTAACATTGTCACTCTATCCGTGTAAGTGTTCCCCTGG20.16220600162206003No Hit
CAGTTGTGGTATGTGTCGGCACTTCGAGCCAATGTGCGGTTTGTGCTGCC20.16220600162206003No Hit
GGAAGTTATCACTGCCTATGAAATAGCCCGATCACAATTCAAAAATGCGA20.16220600162206003No Hit
GGCATTTTTAGGAGATGATCATCTCTATAATGTAATTGTGACTGCGCATG20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCC20.16220600162206003No Hit
GGTGGCAGTACCGGAACATACGTGTGATAGTTAAACAAAGTGTAATTCAA20.16220600162206003No Hit
GCGAGACTGGGCAACGAGAGAAGCCTATTTAGAAATGGACAGACGCGAAA20.16220600162206003No Hit
GAAAAACTGGTTTCATTTATTAAATAAAAAAAGTGAAATGGAATTGTTTT20.16220600162206003No Hit
GTGAAGTATAGCTGGAAGAAAAAAGTCGATAAAACCAAGTAGAAACTAAT20.16220600162206003No Hit
AAAATGATAAGTGCAATATTTTACTTAAAATTGTTTGGTATGGATTTTTC20.16220600162206003No Hit
CTTGACTTCAGAGGGCTATATAATTATCAAATAACAGTTGATTTGCATGA20.16220600162206003No Hit
GGCGACCATGTTTAGAAAACCGAAGCGAAATTTTCGCCAAAGAGTTGTCA20.16220600162206003No Hit
TGGAAGAATTGAGGATGTCACCCCAATCCCGTCAGACAGTACAAGAAGAAAGGGAGGACGTCG20.16220600162206003No Hit
AGAACCTAAAAAAAGGCTAGATCTTTATACAGCTGTGTTGCAAAGCAAGC20.16220600162206003No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16220600162206003No Hit
GGGGGGACGTGGATTAAATCCGGATCAGTAGACGGTACACAAAAAATAAG20.16220600162206003No Hit
GTTTGGCCTGACAGTTGGTCACACATTTAATCATATTGACCTTTATCGTA20.16220600162206003No Hit
GTTTGAACGAGTTCCCACAATAGACCAGTGTTATACAAATGACCCATTGT20.16220600162206003No Hit
CAGAGTCCCATGGGCTGAGCTGTGTAGCCAAATGGGACCAAAACAACCCC20.16220600162206003No Hit
TTTAGACACAGTGAGAACTGATTTAACTAATTTGAACAAGTCAAGCTTCC20.16220600162206003No Hit
TCTACATGTACTTTCTGAGACTACACAACAATGGCCGCCTCTTGGATGTA20.16220600162206003No Hit
CCTTTTAGACTTTATATTTGGTAAAAAATCTGACAAAAAAACTTCTGAAA20.16220600162206003No Hit
TACTTATCCAAGTATATGAGGGTGAGAGAGCTATGACCAAAGACAACAAT20.16220600162206003No Hit
CCCATTTGCAAAGTTCCCAAAAGGAAATTTTGACTTCGATGCTTTCAAGA20.16220600162206003No Hit
TTTTAGGGTGAGGATCGTTGGGGGTTGCACAGTCCTGTGATTTGTAAGGA20.16220600162206003No Hit
TCTGACCGTCCCTGAGCTCACCCAACAGATGTTCGACGCCAAGAACATGA20.16220600162206003No Hit
GTATTTAATATCCATAAAAGCATGACTGTGTTACAATCACATACCAATAT20.16220600162206003No Hit
GGGGAAGTAACTACCTCATGCCAGCTTACTGTTCAGTATGAAAATAAACA20.16220600162206003No Hit
CTATATAGGAGATATATGATTACTTCATTTCCCTTAAACAGTGTTTTCTG20.16220600162206003No Hit
TTAAAGTTCATTTTAATGAATTATGATTCATTATAATGAAAATTAATGTC20.16220600162206003No Hit
CTCGAGTACTGGTTAATCCCAGGAAACAGTGGTTAGGTTAACTGATCGTC20.16220600162206003No Hit
AAGTTGCCGAGACACCCTGGATATAAAGAAGCCCCTCAGAATGAAATAGC20.16220600162206003No Hit
CCAAAACTGATACATCATGTGGAGAACAGACCAACGCTGTTGGTGGGGAA20.16220600162206003No Hit
GGTTCTTTTCAGGATCCCATGAAACAGTGGTTAGGTTAACTGATCATCTG20.16220600162206003No Hit
CGTTTACCATACCTGTGACGTGGCATTATAATCAGTCCGATCTTATCCAT20.16220600162206003No Hit
GGTAACTTCATGTTGGTCTTTACAAGATGACGGTCTTAGAATAAAATATA20.16220600162206003No Hit
TTTGGACCAGGCAACACAGAATCTTCTTAACAGAGGTGTCCGACTCACCG20.16220600162206003No Hit
GTTTCAAAATGCTTGCATAAAACCTATTTTCCCACCTTCTTTCTAAGCAA20.16220600162206003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)