FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-116-6_S45_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-6_S45_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC675.43390105433901No Hit
AAGCAATACACCTTTACAGCTATCAAATTGCAAATCAACTAAAATACTAA100.8110300081103No Hit
TGAAAACCACCATAACAAATAATAATAATAACAATGTACGACATAAATCT90.7299270072992701No Hit
TGATGGTTAAACAAATAAAGAAAAGTAGACAGTTTAATATGATATTTCAT80.6488240064882401No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC80.6488240064882401TruSeq Adapter, Index 5 (100% over 50bp)
TGGAGACCTGCTGCGGATATGGGTACGGTCTGGCACGAGAATCACACTGT80.6488240064882401No Hit
GTCCTTTTCCAGAACAGCACCAAACCTGTATTCATTGTTACAGCAATGGA70.5677210056772101No Hit
GAACAGCAACAACACAACTACAAAGTCTTCCATGACGTCGTAATTTAAGC60.48661800486618007No Hit
AATTATGCCACAAGAACAGATAGATGTTATATTCTGTTATTTTAACGTTT60.48661800486618007No Hit
ATACAACATTTCTTGACAAAAACAAATGATTTCTTATCACAAAACGGTAT60.48661800486618007No Hit
GAAATACGATTCAAGTCACAATATCTTCTTGACTGTTTTAGTCTCAGCAC60.48661800486618007No Hit
TTTATGGTTAAATAACTACTGTGTTACTTTCGTAAAGACCATGTACTTGT60.48661800486618007No Hit
TATTATATCTATAAATAATGGAAATAACAGATTCTTGTCTAACATTGGAC60.48661800486618007No Hit
GACAGCTTGGTGCTTTGAAGTGCAAAATATAACCATGGTTGGCAATGTCA60.48661800486618007No Hit
CTTAAAAGACCACTTGAGACAAAGTATCAGGTGACCTAATATTTATAGCA60.48661800486618007No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCC50.40551500405515No Hit
GTTGCTTGCTCCATCGAAGGGCTGGTTGCTGGCTCCATAGTAGGGCTGGT50.40551500405515No Hit
GTCATGGCAATAGCTCTCCTTGACATTTGTCTCGGCGAGCTAAAAACAAC50.40551500405515No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.40551500405515No Hit
AAACTCTATCCATGTCTATTTCCCTATTTTCTTAAAGACATTTCAAGTTA50.40551500405515No Hit
AAAAAATCTATCATATAAAATATATCATGATCTTATTAAATGTTAAATTC50.40551500405515No Hit
GTACACTGCACACATATACCACTGGTACCCTGTACAAACATACTGGTACC50.40551500405515No Hit
ATCAACACGCAACTTAATTCTTTTATAGAAAAGGGGTTATCAAATAGCTC50.40551500405515No Hit
CGAATACTAGTGACTCTTACATCATGGCCTTAAATCTTGGTTTTCTATTG40.32441200324412006No Hit
GATTCACGGAATTAAATATGCCCGATTTGTTTCTCATTCCTTATAGAGAG40.32441200324412006No Hit
TTTTTATGTACATGTATTTTATGTATTTAAATAAAGTTTTTAATGATTTA40.32441200324412006No Hit
GCGACATTATCATATGTGACATTATTATATGTGATATTATCATATGTGAC40.32441200324412006No Hit
GCATAGCTGGCGCCATTCACAAATGCCACGACTGACGCCGACGATATTGT40.32441200324412006No Hit
GTGAAGTCAACTTGGACATACTCTGTCATGTCATCCAGGCCATGAATCAC40.32441200324412006No Hit
CTATTTTGTTTATTTTGCACAATAAAAACAATATGCATAAAATGGTGTCG40.32441200324412006No Hit
GGCTTGGTTATCGGCTTCCATTCCTTTTTCAACAATTTGGCCATCTGTGT40.32441200324412006No Hit
GTGATGACTTGACCGTCGGGGAGTTCGTAGCTCTTCTCAAGAGAAGATGA40.32441200324412006No Hit
CCATCTTCCAACTGCTTTCCTGCAAAGATCAATCTCTGCTGATCTGGGGG40.32441200324412006No Hit
TTTCCATTAGCACCAATATCACAGCATAAAACTTTATACATTAAATCAAA40.32441200324412006No Hit
AAAAGCTTGGATATATGATAAAAACAACAACAACATGAACTCATTAGCGA40.32441200324412006No Hit
GGCAGTTTGCCTAGCTCCATCAGAAAATGACCCTGATCCACACTCAAAGA40.32441200324412006No Hit
GGTCTTATAGCAGGATCTGTATTGTGTGATTGAGCGAGCACTTTATTCTA40.32441200324412006No Hit
CAATGATGTCATTCTGTTCCAAGATGGAGATCTCAGGAACGTAATTATCT40.32441200324412006No Hit
CACGGCCTGGACGACCACCGCCATATGGTGAGGTGAGGGCAAAGTCAATG40.32441200324412006No Hit
TTATACCACATCGTTAGAAGCAATGTAAAAAGTATGTTGTACAAGTTAAA40.32441200324412006No Hit
CAGTTGGTGGCTGGTAGTTGATTCCGACCTTGAATCCAGTTGGACACCAG40.32441200324412006No Hit
GAAAAATGTATTTCCGTTTGTGCTATACATCACCATCTAAATAATGGCTA40.32441200324412006No Hit
TCAGAGTTCAGAATAAGGTCAGTTTGTTGTACCGTGTGATACATCTATGA40.32441200324412006No Hit
GTGGTAAATGAAGGATGCAGACCTGTCGGCGATGAATCAGCAGAATGAGT40.32441200324412006No Hit
GGATACGTGGGTATGGTACCAAGTTGGTCTGGAACTCAGTCAAGTCGACG30.24330900243309003No Hit
TGCTCAATAGCTAACGTAATCTCAGACAGGTAGAAACATGCCGTGTCCTC30.24330900243309003No Hit
CTTTCCTCTGTGCAAGGGCCACGTATAAAGGCTTGGCCACCACGATTCTC30.24330900243309003No Hit
GACAATGTTAACATTGATACCGGGGGAACACTTACACGGATAGAGTGACA30.24330900243309003No Hit
CTTACTTAACATTACAATTTCAACATTTATTTAGTTCTTAAAATTGTATA30.24330900243309003No Hit
TTTGCTGGCAAGGTTACTAATCATTATAATTAAGGTCAAAGTTCAAACTG30.24330900243309003No Hit
GAGACTTTTACCATCACTTTCGACAGCAACGTTGATGGGGAAATGGGCAT30.24330900243309003No Hit
AATCCAATGCACATACTTCCGAATACACTTCCGATTCCAGCACCTGATCC30.24330900243309003No Hit
GTTGCTTAATTGAATCCTCTTCAGAAGTCACTTGGTTTTCAGATTTTTGC30.24330900243309003No Hit
TCGCATTTCATTTTGAAGAACCAATTTTTTTTTCTTCATCATACCGTGTT30.24330900243309003No Hit
CTTTGTTCTCGGTGGCATCACCGATAAAAACTTGTTTTCCGTCTGAATGA30.24330900243309003No Hit
GTATATGCAGCGAACAAATAAGTCGTGAAAAATGCCACAGCCTTTGTTGC30.24330900243309003No Hit
CCACACTCAAGCGTTCCATGAGCAGTGAAGCAAAGCCTGACCCGGTGCCA30.24330900243309003No Hit
CCTTGACGCATTACACTTTTGAGTTCGCGAATCTGTCGACAATTGTGTAT30.24330900243309003No Hit
ATTTTGCCCACAATTAATAATTTGTGTAACAATGAATAAAACCTTCTGAA30.24330900243309003No Hit
GGACTAGTCTCGTAACTTAAACTTGACAGAATTAACTTAATATACAAATT30.24330900243309003No Hit
GTAGTAACTTGGATAGTGCCCATTACCTCTGAGTAAGCCCAGCATCAGAG30.24330900243309003No Hit
GGTGGGTGACCTCACTCGCAGTATGTCCTGTAGGGAGGGCAGGTAGTCTG30.24330900243309003No Hit
CAACTAACCATACTGTCTCCCCCCCGTCTAGATATATCACACACAACTAA30.24330900243309003No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.24330900243309003No Hit
CGACCGTTCCGCTGTTGTATTCGAATGTGGATAAGTTTGTCCTGTGCCCC30.24330900243309003No Hit
GTTTGCAGTGCTACTACCTAATCATTCAAAGTTGCAGTTACCAAAACTTT30.24330900243309003No Hit
CACCTTTGACCAAAAACTGATCAACACCGACCAGACCGCAAATGTTTCCA30.24330900243309003No Hit
GTTTTTTTATTGTGTTTGAACTATTTAACTATTCTACAATCGATCCAGAG30.24330900243309003No Hit
CTTTTACACTCTACTGGAGAACATCTTGGAATTTCCTTCCAAGTATTCAT20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCCC20.16220600162206003No Hit
ATTTTTCCCTTCTTGACTCTCCCCAATAATAGTTACACTCTGTCCACCTT20.16220600162206003No Hit
CACAGATCGACCTGATTCTCCAGGTCCACCTTCTTCTGCTTGTTGAACTC20.16220600162206003No Hit
CGATGCTCTTCCGCATGACCACGTGCTCCATATGATTACAAAACCAGAAA20.16220600162206003No Hit
CAACTTCGTCACCCAAATGTTTATATAATAATGTCCTCATCGCCTATTTC20.16220600162206003No Hit
TGGAAATACAGCTTTGTGCAGATGGAAATACAGTTTGCCAATGATGCAGC20.16220600162206003No Hit
CTTCACCACGCCATGCCTCGGCCTTGGCCACTATGTAATGAAGCTCCTGA20.16220600162206003No Hit
CACACATACTACTGAAGGCATTCTTCCTCAATTTTTCTATTCGTTGGAAG20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGCCCCCCC20.16220600162206003No Hit
CACAGTTGTAGAAATGAAATTAATAGCCCCCAAAATAGAAGATACCCCCG20.16220600162206003No Hit
CGACGTCCTCCCTTTCTTCTTGTACTGTCTGACGGGATTGGGGTGACATCCTCAATTCTTCCA20.16220600162206003No Hit
CTGTGTAGTGGTGAACTCCGGTCTTGGCCAACATAGCATAGTATTCAATT20.16220600162206003No Hit
CTTAAACAAATTACTATTACCACTATTAAAACTATAATCGACGTAATCAA20.16220600162206003No Hit
CAAAACTGACACAACATTTATTTATTCTTAATATTAATGAAATAGCTTCT20.16220600162206003No Hit
TGAACATCTCCCTGACAAAGTGCTGTATCACAGTTGCAGCATTACCATGG20.16220600162206003No Hit
TTTTGATCAAACAATAGTGATATTTAAAAAAACAAATTTCATTATTTCTT20.16220600162206003No Hit
GTTAAGGGTAAAATAAAATATTCTCATCAAACTGCTATTTAAAAGAAACT20.16220600162206003No Hit
GAGTGATATAACTCCTGCACCATTCCTATTGGACACCTTATGAAGACCAA20.16220600162206003No Hit
GAGAAGTTTGGCTGTAAAAGCACCCATGCCCATGTTACCATCATCTTCAA20.16220600162206003No Hit
GTGTCAACCAATTATTTATTAAATTGCAGTTTCACCAAATAATAAAATTA20.16220600162206003No Hit
ATCAATTTTATTTGTTCCTCATCAGCTAACTGTACTGCATAGAATTTCTA20.16220600162206003No Hit
TGTTAAACCCCACACCATCTTACGTGCAATACCACTAGTATTATATATAA20.16220600162206003No Hit
GGAAGTTGTTGATAGTTGATGTCGGAAGTGAGCTGGTTCGACATCAAGCA20.16220600162206003No Hit
CTCATATCTGCTGGAACAGTATGAAAATATGCTTTATCTGTTTTAGATGG20.16220600162206003No Hit
CGAAGCAACACAAGGAGAAGGGCAAGTATTGTCATAATGCTTGATGTGAT20.16220600162206003No Hit
GGAACTACTTGACGATCTATGACTGGTGCCCAAACCAGATGATGAACCGG20.16220600162206003No Hit
GTGAAACAGTAAATATCTGAAATGAGACTAGACTCAAATACTTTTTTTAC20.16220600162206003No Hit
CGAGAAAACACGTATCTATGGAGTAAAAGGTTAAAGTCATTTTAAAACAA20.16220600162206003No Hit
TCTAGATCAGGTCAGCCACATTCATGGGCATTTCTTCGATTTGAGTGTTA20.16220600162206003No Hit
TGCTATACAATCAAAGAAGTAAATATATAATAGATGGAATCCTTGACGAT20.16220600162206003No Hit
ATAAAATATAAATATGAGAGTGAAATAAGCAGCAAAAAGGAATGAATTAA20.16220600162206003No Hit
CTTTAATTGCCACTTTTTGTAAATCACATCTACTTCGTTGTTTTGACTTC20.16220600162206003No Hit
CGATCTCCTTCAACTTGGCATCATTTGCTTCGCTGATCATTCCTTGTTCC20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCC20.16220600162206003No Hit
CCGATCAGTCTGTTCAAGTTGGTGTATGTTGGTCTCTCAATGTCCAAGTT20.16220600162206003No Hit
CAAGCTCTTAGTAGAGTGGAAGTAGAGAAAAGTATCATACAGGAGTTTGC20.16220600162206003No Hit
TAATATTTAAATCATTGATTATAATTGTATCATTCATCTTTTTATGATGA20.16220600162206003No Hit
ATGTATAAATTGTATTATCAAATAAGGCACTAAAACATTTTTCAGTGCAT20.16220600162206003No Hit
CTTGACGAAGATCTGCATACCTCCTCTCAATCGAAGTACAAGATGGAGGG20.16220600162206003No Hit
CAGTATTGTTACTCTCTCGTCGAAAAAGTTTATGTCGAAAAAGTTTATTA20.16220600162206003No Hit
CCCCAAACCACACATTGATTGGAAATTGAATCACAATAGTGAACATGACA20.16220600162206003No Hit
CGGCGATCATGACTGCAGGTGCGTTCGTATTACCGGTTGTTTCTTGTGGC20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACC20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGC20.16220600162206003No Hit
AAAAGAAACATCATTTTTGTACGAGTTGATTTTACGGAAATGGTTTAAAG20.16220600162206003No Hit
CAACATCTGTTCGTCAACCTCCTTCATGGACATACGTCCACGGAACATGG20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCG20.16220600162206003No Hit
TGATGGGTAAACAAATAAAGAAAAGTAGACAGTTTAATATGATATTTCAT20.16220600162206003No Hit
TTTCACTGGGTTCTTCCCTTTCAATAAGATCCCAAGATAGGTCCCCTTCA20.16220600162206003No Hit
GTTGTAGTAACAAACGGTTATTACCGACGCATTGATATCCATATAAAGCG20.16220600162206003No Hit
TTACTCAAAAATGACCATTTGATCACACAAGAATTGGAAACTATAAGTTC20.16220600162206003No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCC20.16220600162206003No Hit
GGCATCTGTTGGCGTAGATAAATTTTCGTAATTACGCGATTCATACTAAA20.16220600162206003No Hit
GGATACCGTTAGCATCTATATCAAAGGTAACTTCAATTTGTGGGACACCA20.16220600162206003No Hit
CTTGGCTTTCACATTCTCAATGGAGTCAGAGGCTTCAACCTCAAGGGTGA20.16220600162206003No Hit
TTACAGATGAACTTTTATTCCATTTATAAAACTACACACACATAACACAT20.16220600162206003No Hit
TTTTAACGCATATTTAAGGGCGGCTAACGGAAATGCATTTTATCAATTTA20.16220600162206003No Hit
TTATTTCTCAGGCACAGTAAAACATGAGTGAACTGAAAAAAGAAAATATT20.16220600162206003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCTTGTAATCTCGTATGCCG20.16220600162206003Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ACAACATGGTATAGGTTTGGGGCGGGTGGGAAAGAAAAAAATATGTACCC20.16220600162206003No Hit
CGTAAACGTATCAGGCTTAATGTCGAGTGAGTCTGTGCTGTCTTCAGTAT20.16220600162206003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)