FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-116-2_S13_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-2_S13_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589
Sequences flagged as poor quality0
Sequence length68-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT50.3146633102580239Illumina Single End PCR Primer 1 (100% over 50bp)
AACATATATACCACAAATATTAAAATATATGTGCAATTATTTTGTAACCT40.25173064820641916No Hit
CGTGGAGGTTATAGAGGTTATAGAAGAAGCAAATATTACTACTAGAACAC40.25173064820641916No Hit
ATCGGTTTGTATGGTTGGTTATATTGTTTGTTCTTTCTATAAATTTATTG40.25173064820641916No Hit
GCAAGTCCCCCGAATTCTCCCAGAAGTTGAACGCCACATGACTTTTCAGT40.25173064820641916No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG40.25173064820641916Illumina Single End PCR Primer 1 (100% over 50bp)
CTTACCCATACGTAGCAAAGGATGGAACATGCAAATTCAACCCATCTAAC30.18879798615481436No Hit
TAGTCAGCTAGCAGGATTGATTCAGTTATTATCTCAATTCTTCATACAAG30.18879798615481436No Hit
CCGACCAGTGCCGTCTATCATAGTACCAGCGTTTTAGATAACATGGCCAG30.18879798615481436No Hit
GAACACAACAAAATACTATAATACTGCATGATAAATAGTGTGACCTTATA30.18879798615481436No Hit
TGTTTATACAAAAAAGCTGGTATATTAAATGATTTTTTTAACTCTTTAAA30.18879798615481436No Hit
TTTATGGCCACGTTCCCAATGACTACGCCAGCGCCGGTAACTCTGTCACT30.18879798615481436No Hit
CCGATGTGTTGATGCAGCTATAGGAAAATATCAGTGTGATGAAAAATGTC30.18879798615481436No Hit
AGTTACTTGCAGAGATGGACTTGCTCAAGTTCCGGTTAATCTGCAAGGAT30.18879798615481436No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.18879798615481436No Hit
TTAATATTAGATATGTTCCCTGTGCATGTTGCTCAGTTGAACCAGAATTC30.18879798615481436No Hit
GTTAAATCGATCTTGAAATTTCGAAATTATTATATATATATAAATATTAT30.18879798615481436No Hit
GAGGCTTACGCCAATTTTGAAATTTGTTTTACTTGTCGGATAAATATTTA30.18879798615481436No Hit
CAATTATACATTATATACTTCTTCACTATGCACCATGTCTCACGTATACA30.18879798615481436No Hit
CTCAAACTGACCACCCCAACATACGGTGACTTGAACCATCTCGTCTCCGC30.18879798615481436No Hit
ACTCGGAACGTTTATGGATTATCCTAATGTAAATAAAAATATATTCACAT30.18879798615481436No Hit
GACAAACATTGCGAAAGTTCTTTGAAAATAGTACAGCTAAGTTTGCATTC30.18879798615481436No Hit
CCGGTATATCCAACATGAATAATTCAAGTGCACTGTTCTTCATTACAAAT30.18879798615481436No Hit
GGTTTCTGATGCTTTTAACTGTGTGACTACCTAGGAAGTGAGGATTGACT20.12586532410320958No Hit
TCCACTCAACACTGGAGTATTTGATAAGATTGTGAGTAAACATAAGTCTG20.12586532410320958No Hit
CTGCACATAGTGAACTGTCTGATCAAAGTTGACAGTGTTTGGCTCCAAAT20.12586532410320958No Hit
CAGAACGACGGAATCCTTCTATACGAAACCAGTGACCTATACTACAATAT20.12586532410320958No Hit
TTCAGGATAGGGCTTCTTTGACTATAAAGAATTTAGTTCTTTATCATAAT20.12586532410320958No Hit
GGAAGGTACATTTTTGGGATGTCATACCTCACTTGTCGTGCGAGGGTTAC20.12586532410320958No Hit
GAGCGTGATGTCATAGCAGAAAGCCATGTCGACTTGAGACATAGGTATCA20.12586532410320958No Hit
CGGAGCTGCAGCTTGGTGGTTTATGGTATATGAGGAGGGACCCAAGCTGA20.12586532410320958No Hit
CTCAATGCAAGGAAATATTCTGCAAAACAAATAACCGGGCAACGAAGTTT20.12586532410320958No Hit
GAAACGCCGATGGTACCTGAATGAACGTGAATATAGGTTTATTGAAATAA20.12586532410320958No Hit
GGAACATGTTCTACAGCTGAAGCGTTCCGATATGAAGTCGGTAAAAAGTA20.12586532410320958No Hit
TTAATTGTTTATAGTAACTGGGTCAATTTTCAATTGTTTTATTTATGTAA20.12586532410320958No Hit
GTTGAAAAATGGGTGTGCAATCACGTTGAAATTGTTTGTCCTCCATCCGT20.12586532410320958No Hit
TTATTAAACAAAAAGGAATAAGAACAAGAAAATCAGTGAATTTAAAAAAA20.12586532410320958No Hit
GCCCCATCCCCAGAACTGGCTCCCCAGACCGTAATGCCAGCCCAACCAGA20.12586532410320958No Hit
CTTGAACAGGAAGAGGCCAAGGTTTCGCGTTCTCAACTCGAAATTGCAAC20.12586532410320958No Hit
GCTCGGTGGTTACGATATTTTGGATCTGGCTAAACAGCTGTTGACACAGT20.12586532410320958No Hit
AGTGGTAATAGTAATTTGTTTAAGGTTGATTTGTAGGGATAAGGATTTAA20.12586532410320958No Hit
GGCAGTAAAAGCAATAAATGTGTTTGGACCCTTCCTTTAAATTTACATGA20.12586532410320958No Hit
GAAATACAACGTTGTGTTGATATTAACTAACAAAATAAAAACAACAACTT20.12586532410320958No Hit
GGAAGAAGACGGCTATATACTTGGATGTTGTGGAAAGATGACAACAAGAA20.12586532410320958No Hit
TCAGGCTGTAGTCAGTATGTTGTGTAAGATGTCTGCGTGTCAAGTATTTT20.12586532410320958No Hit
AGAACATCGCTGATTACAAGAGTAGAGGACTTTGCCCACCAAGCGCCGAG20.12586532410320958No Hit
GTTGATCTTATGTTTGTCACTGTAGGATTATTCAAAACAATGATTATTGT20.12586532410320958No Hit
GTTTTTTTGAAAAAATGCGGGTTTTGAAAAAATGCGGGTTTTGTATGGCC20.12586532410320958No Hit
GTTTTCCACATTTTTCCAGATTATCACGGAATCATTGCAGTACAAAGCCC20.12586532410320958No Hit
CTTGTACCTGTCGTATCATGATGTTCACAGAATTCTTGATTAATTTTGTA20.12586532410320958No Hit
ATAAACTCCAAATGAACCCACCTATCCACTGTATAAAAGATTTATATAAT20.12586532410320958No Hit
AAAAACGCTCAATTGAACCGGAAGTGACACAAGTACCGGAAGCTGCGTGA20.12586532410320958No Hit
TGAATTTGTGGTAGTCGGTGTTATGCTTAGTCAACGTTTAACTTAGAAAC20.12586532410320958No Hit
CGCTGTTGCATTTAGAGATAAGAAAGTACAGTTTGTCGCCATTGGTAAAT20.12586532410320958No Hit
GACTACCAAAATTGTACCTGCATAATCAAGTCTGTGACAGTTTGTTTAGA20.12586532410320958No Hit
CCGGACACGACTGACCCTGCTGTGTCCAAATGCCATTCCACACAAACGGC20.12586532410320958No Hit
GTTTGCCTTCTCAAGACAAGATAAAGGGCAGTAACTGTATGCAACTACCT20.12586532410320958No Hit
GTACAACTAAGGCCAAGAATGTACTGGGCTTTAGGGTTTCTATTTCTTTT20.12586532410320958No Hit
GGTGCCGATGAAGAAAATAGCAATAAATCAAACAAAAATGCCAAAAATAA20.12586532410320958No Hit
AAACATGTTTTGTGGAGTGACACGAGCGCATGCAGTTCCTCAAATACTGC20.12586532410320958No Hit
AGTTTGAAGGCTTGCCATTCGTAGAGAGACCCAAATGAAAGAAGTGAAAC20.12586532410320958No Hit
CAAGCACCTCTCACATATACATTCTCTATAACCCCACCGTCTCTCACATA20.12586532410320958No Hit
GAAATTTTGTGTACAAAATGTTCAAAAAATGTTAAATCATTGATAATATA20.12586532410320958No Hit
TGTACATTATGCCCATGCATGTATATATACCCTAATACCCCACCCACCAA20.12586532410320958No Hit
TGAAGAGGAAGAATGCCAGCAGAAAGAAAAGGTGCACATGTTGAACACGT20.12586532410320958No Hit
GCAGCACCTAGTGATCTTGACACCCAATCACTAAAGGAAGAGAATGAGAA20.12586532410320958No Hit
TAGCGTGCGAGACCCCAACAAGGGTTTAATTGCGAGGGTTTTATTAACTT20.12586532410320958No Hit
GGAATGGATGCCCGTCACAAAACTGGGTCGTCTCGTAAAGGACATGAAAA20.12586532410320958No Hit
CCTTTACTTCATATAGACACCTTTGGACACCTTATATAGAAGGCTTTTTA20.12586532410320958No Hit
GGGACACATGGCCAGAGATTGTCCTAACGGAAAAAGACAAGACAACAGAG20.12586532410320958No Hit
AACACTCTAAGACACAGGTACCGGTCACAGTCTGTTTTTGATATAAATGG20.12586532410320958No Hit
GCGGCGCCAACTCTTGGTCCACAAACCGGACATCCAGAAACTCCATCTGC20.12586532410320958No Hit
TTTTTACTATATTTGCCCACAATGCATGTTTTTATTTTAAAGCTTTTTGG20.12586532410320958No Hit
TTCAGTTGAGGATATACAGGAAATGTAAAGTGGCGGGTTAGCTGTTCTGT20.12586532410320958No Hit
CCACGCCAGATTTTCTTATTATGCAGTTTGGCGCATTTTACACAGGACAT20.12586532410320958No Hit
CTGTACAAACATCTCAACAATATTGTCATTTTGACAGTGATGACATAATA20.12586532410320958No Hit
CCCATACCCACGCAACTTAAAAGAGAAAATGCCGATGCTGCAGGGAGGTT20.12586532410320958No Hit
CGACGACCATCAGTAAAAAGAGAAAGTTTGTCGCTGATGGAGTGTTCAAA20.12586532410320958No Hit
GGATTGCCGAAGGGAGATCTTATGGAACTTATCAAAAGTAATTTCGGCTC20.12586532410320958No Hit
CGACCCTTCAGGGTCACGGGAATTGGGTAACCCAAATAGCTACAACGCCC20.12586532410320958No Hit
TGAAAACTGAGTAAACATGGTTGAATCATATGCAAACTTGAAGAACTTAG20.12586532410320958No Hit
CCGATAGGCTTCAGACTGGGATGCGTGGTGCGTTCGGTAAACCTCAAGGT20.12586532410320958No Hit
GCACAATCCAGGAAAGGGTATTTCCCAATCAGCTCTCCCATACAGAAGAT20.12586532410320958No Hit
GGACACAGCCACTTTGTTTCTGATGTTGTCATGTCCTCAGATGGCCAATT20.12586532410320958No Hit
TACCCTAATATTGTTGAGACCGGACGACCTTATTTTCGGAGAACGGACAT20.12586532410320958No Hit
TCCACATAATGGCTGACGGTAAAGCCATCACAATTCAATCGGACGGACAG20.12586532410320958No Hit
AAAGTGTAATGTTTGATAACAGGCATGGTTTGGATTTCTTACGGTGGTTA20.12586532410320958No Hit
TCTGTATCCTACTTCAGAGTAAATGGCGTTTTGTAATTTATGTTAGCTGT20.12586532410320958No Hit
TACACTGCCAGATATTTCGATACATTGTAAAACATTTCAAAATAAATACT20.12586532410320958No Hit
GCCATATTTTCATTTGTCTGCCAGAAATGCAATCGCATTTTATGTCATTT20.12586532410320958No Hit
TTTGAACATGTTCACCCATTTTAAGAAAAAAAACTAAACTCTTACTGATG20.12586532410320958No Hit
GAGTGATTGTTACAATTAAAGTGTAAATGTGCATTACTATTGATACAAGT20.12586532410320958No Hit
GTCAGAACTCCCCAGCCGGATGATAAGGGAATGAAGAGAAAGTTGCTGTC20.12586532410320958No Hit
GGAAGTGATAGTGGAATGACATATAGCGGGAATAATAGTAAAATCTCATC20.12586532410320958No Hit
AAGACCAGCAAAATTGGTCTGAAACACAAAGTAACAAAAGAGTCCTCCAT20.12586532410320958No Hit
TCTGTCTGCACACTTTGCTCCAAGTGTTGGCGGCAAGTAGACAATGACGT20.12586532410320958No Hit
TCGCACTGCATTAAAATATAAAAAAATAAAAATTGTGAATGTAGACTGGC20.12586532410320958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)