FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-116-2_S13_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-116-2_S13_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589
Sequences flagged as poor quality0
Sequence length68-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.4405286343612335No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.3775959723096287No Hit
CGCATGAACAACATACAAAATACAGCTTCTGTAAGTCTCTCCATTTCCGG40.25173064820641916No Hit
TTTTTTATTATTAGGTTCAATTAATATTTTATTTTTAATATTTTTCTTTG40.25173064820641916No Hit
GACAATTAACCATGTAAATATATTTTTGGTCACATTAAATTCTAAAGACA40.25173064820641916No Hit
CAAAGATTCCTTATTCTGATAATAGATCACCAATAAAAATGACACAATAC40.25173064820641916No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC40.25173064820641916TruSeq Adapter, Index 5 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC40.25173064820641916TruSeq Adapter, Index 5 (100% over 50bp)
TGGAATGCACTTGAACTATTTGTAATGATGAACAGTGCACTTATCTATTT30.18879798615481436No Hit
CAGAGCTCTGTACTGCTGGCTACCACGAGATGTAAGAGGAGCGAATCCAG30.18879798615481436No Hit
TACATGTTCGTGTCGTAGCCATAAGTGTTATCAACCGCCTTTACGGATAC30.18879798615481436No Hit
CTTGTCCAGTGATCCCACCAGAGGTTGGGGTAAAATCACACACTGGTTTG30.18879798615481436No Hit
AGCTATTCATCAACAGTAGTGTTTAAAAACACTGAGTCTATTTCTGAAAG30.18879798615481436No Hit
TTCAGAAACGTTGACAGTATGTCTAATCATCGTTTATTCAGTGAAATCAG30.18879798615481436No Hit
CTCTTGTAAAGTTGGAACGATTTGTGTCCGGCGTCAATAGCAACGGAAAT30.18879798615481436No Hit
ACAATGTCATCCTGTCAAATCCAGCGACGCTGTAACTAGGTAAACCATGC30.18879798615481436No Hit
GCCGAGTCTAAAACATATAGTCAATAGTGAGTATTTTCATCAAAACGTGA30.18879798615481436No Hit
CGCCATTTTCAGTAAACAATGTTCACTTAAAACGCGTTTTAAACTCTACA30.18879798615481436No Hit
AAAAAGGTTTTCAGCATTTTTAAACAGGCTAGCAACTCTTTTCAACTTTT30.18879798615481436No Hit
CTTTCATGATTCATTGACAAATTCTTTTCACATACATGTACCTTATAAAT30.18879798615481436No Hit
TGCAAATATAGCAAATACTGCCCCTATACTTAAGACATAATGAAAATGTG30.18879798615481436No Hit
CTTGTCAAATAAACAATTCTAAATACATTAATAAAAACGACATATTACAT30.18879798615481436No Hit
CACAAAGCATTTACCAGAGTATGAAAGAGACAAAATACTTGACACGCAGA20.12586532410320958No Hit
TTATTCTCTTAAGTTTTACAAGTAATGACACTACTTTCCAGGCAGGATAA20.12586532410320958No Hit
GTTGATGGAAACTATCCTAAATTTTCGATGATATTTGTGCATCTATACAT20.12586532410320958No Hit
CTTCAAGATTCTCAAAATCTTGTTTCCAGTAACAAAACGTACTTGGGCCA20.12586532410320958No Hit
GCCATTGTCATTGGTTCGGGAGCACTGAATATGCTACAGTCAAGACCCTG20.12586532410320958No Hit
AGCACACTGTTCGCAACAGACGAGATGGCCACAAGGTAAAAAGGTCGTAT20.12586532410320958No Hit
AATGCAACAACAATACACCATTGATACCACATTTATTTGTTTATGTTAAT20.12586532410320958No Hit
GCCCGTTTTACTGACGTTGTAAGTTACAAACTTCTAAATGTGTAAAATAT20.12586532410320958No Hit
GTTGCGCATCTGGCAGACGGCATCATCCCTTTCTCTCTCGGCATTTGCAA20.12586532410320958No Hit
GTTCAGCCTGCTTCAAGTCATTGTCAAATTGTTGTAGAGCTTTCTTGCAG20.12586532410320958No Hit
GCCTCGTTCAAGGCTTGTCTTGTGGTGTCGAGACGTTCCTGCAGAATCCT20.12586532410320958No Hit
CTGAACAGAACAGCTAACCCACCACTTTACATTTCCTGTATATCCTCAAC20.12586532410320958No Hit
GTGGAGGTATTGAAGAAGTATGTAATGTATAAATGTGAGATAGTGTGTGT20.12586532410320958No Hit
TTCTGTTTGAATTTTCATTATTCTCTCTTTTTCTTTTAAAGTTTATTTCA20.12586532410320958No Hit
AAACTGATTGCAAATATAATAATAAATCTAATTTCCTCACATGGTTCAAA20.12586532410320958No Hit
CAGATAATCTGTTACTCTTAATACTCGAGCACACCCCATCTCCACAAGTA20.12586532410320958No Hit
GTACTGACTCATCCTCCATTCGCATAATCATGGCTAACTGCTGACGCTCT20.12586532410320958No Hit
GTAAGTGTCTAAGTCTTTGAAGTAGTCGACTTCAAAGCTTCCTGCGCCCT20.12586532410320958No Hit
CTTTAATAGTACTGGTACCGTAGGAACACCTTATATTGTGTTACGTATCT20.12586532410320958No Hit
GCCGAATCAAGCTGGTTCAAGAACTTGTCTGAGATTGCCCAAATCAATTA20.12586532410320958No Hit
TCACAACGCATATCAAAAATAAAAAGTAGAATGAACCGTTACATTGCTTT20.12586532410320958No Hit
TTCTGTTATGAACATTTTTGTTCAGATACTGGGTTCTGTTGTGTACATAT20.12586532410320958No Hit
CCGGTAATCAATGAGTTATGTCCATCAATTTTGTCCAACAATTGAGGGCA20.12586532410320958No Hit
GGATCATGTATACGTGAGAGTCGGTGGGGTTATGAAGAATGTACATGTGA20.12586532410320958No Hit
GCCAAGTTCAACACCGAGTGATGACTATGTAATGCTATTACAATAGATTA20.12586532410320958No Hit
AACATTGTACAGATGTCAACATTGTTGGGATGTAGACATTGTCTAGATGT20.12586532410320958No Hit
AGTTTTTTTCAGTGTATTAGGTAAATATTGATACATTTAGACATCACACT20.12586532410320958No Hit
CATCATTCCACTTCTGTATGACAGCTGACAAATGGCATCAATGCCATTCC20.12586532410320958No Hit
CATAATCAACGATCAACATGGGCATTTTTCTTTCATAACAAAAGATGTCC20.12586532410320958No Hit
TTGCATGAAAATTTGTTATTTATTGACAACTATAACATCGTTCTTCTACT20.12586532410320958No Hit
TGACTTCTTTCTGAATGCAGCCCCAGTTTGTCTTTAACAATATACAGTCA20.12586532410320958No Hit
AAGACATCGGTGTGATTTTAATATCACTTGATGGATGATCAACTGACAGT20.12586532410320958No Hit
AAAAAAACAACAACAATAAAAGGATTTTGTTTCTGATATCTAGCGCCTAC20.12586532410320958No Hit
CGCCACTGGATCAGAGTCTTGTCTCTTGATGCCGACAAAATCATGTCGGG20.12586532410320958No Hit
GGAGTCAAGTGATCTCTGGTGGTTACGTCTGGTGTTTTCAAATTCCTCTT20.12586532410320958No Hit
CCGTTGTAGAGTCAAGTACTTGTCTACTCAGTCAGTGAGAGACTATCACA20.12586532410320958No Hit
GCGAAAGGTGTTTTAGGGTCAAACAAACCTGGTGTATCAACAACGACGTA20.12586532410320958No Hit
GCTACTTCCTTGGAGCACTTCACACCAAGACCAACATGTCCGCTGTGGTC20.12586532410320958No Hit
GTTAAGAGTTGTTCAAGCCAAGTGTTGTACATCATCATACCATACATCGA20.12586532410320958No Hit
GGGGTTTTGAACTCAAGACCCATTGATTGATAGCCAAGCGCGTAACTGAC20.12586532410320958No Hit
AAACACCTTTGTAACATGCAAACTGTCATGTTTGATGGCATACGTACCTA20.12586532410320958No Hit
AAGACAGACATGTGTACACAACACAGTCTGTGATTAAATTGTAAATTGCA20.12586532410320958No Hit
GGAAAACGCCTATAAATGGAGTAAAGGGTTAGGTATGTTTTTTAAATAGC20.12586532410320958No Hit
CTCAGCCAAAGATCCTAACCTAAAATGAAAAAAGGACCAAAAAAATGAGA20.12586532410320958No Hit
GATACTTGAGTTTAGTATTAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG20.12586532410320958No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAGA20.12586532410320958Illumina Single End Adapter 1 (95% over 24bp)
GAGACAAGTACCTGTAGTTGCCTATCTCTGTTTATGTGTTTCTGCTATAT20.12586532410320958No Hit
TTTTATTTTCAATGATTCTTAACATGAAGATCAATGTACAATGTGCTAAA20.12586532410320958No Hit
CGAGTGAACACTGCATTATCTCCCCGGCACTGTACGATGGTATTCCTGAT20.12586532410320958No Hit
CCAATCTCTATTCTCTGTTGGGGCATTCACGTGCCATATGGCCTTCTCCC20.12586532410320958No Hit
CAAGCGACCATGCGTTATCCGGGGATTGTGTCTCGGTGTCCTGAAGTCTC20.12586532410320958No Hit
CAGGATTTGTGTGGCTGTTGACGCCAATCTGTATACCGCGATTCATGAGT20.12586532410320958No Hit
GTCTTCCCTTCTCTCCAAGAACATTCTGTGTTCTGGTGGCCAGAATAATG20.12586532410320958No Hit
ATAATAACAAATAATAAAGTGTGGTATTTTTTCCAATATATGATATAAGT20.12586532410320958No Hit
CTTAATTTTGCTTCTTTTTTAGCATTGGAAATGACTGTATTTGTGCTCAT20.12586532410320958No Hit
GGCACATGTTGCGATTCTTAACCTTCCACTGAGGGGGTTCCAACCCAGCC20.12586532410320958No Hit
CGGACTTGTCATAATTAACAGTTCTTAACAGTGTGTCTGACTTATCGTAC20.12586532410320958No Hit
GCGGTGTTTAAATATCGAAGGAAAAGTGCCCACTTCAGGTAACATTTTTG20.12586532410320958No Hit
AAACAGTTATGGTGCAGAGTATTTTATTTAATAGAAAACAAAAATCAGGT20.12586532410320958No Hit
TAAAACCTTACCAAGCCTGAAACACTAAATACCCCTACACAAATAACAAG20.12586532410320958No Hit
CCAAAAAGCAATCTGAACACTTCCTGCACTGTTTTCCACTCTTTCAGTTA20.12586532410320958No Hit
TATAGCATAATTACGTAATTAATATCAGTTGTATGAATGTATATAACAGA20.12586532410320958No Hit
GAATGGATTCATTTTAACAAAATAAGTATGAATACCTGGAACTTTTTACC20.12586532410320958No Hit
GTCATCGCACTGTTCTTCCTGCTCATCAGCTTCTGCAGATGCGTTACCTC20.12586532410320958No Hit
GTTAACTCTCATAGATATGTTTCAACATTACAACTGCAGTCTAACATGCA20.12586532410320958No Hit
GTTTTCAGCATTTTTAAACAGGCTAGCAACTCTTTTCAACTTTTCAAACT20.12586532410320958No Hit
TTTGTGAAGCCCCATTTCTTGGAAACAAAAATCTTTTGACGTCCGGGGAA20.12586532410320958No Hit
TTTTTTTAATACTCTATCTTTATTTGCGGAATAACATCGACATTGCAACT20.12586532410320958No Hit
TTTCAGTAAGGTGTTTCCGGGATTTTGATATTTGTTTCTGTATTTTCAGT20.12586532410320958No Hit
CGAAACATGATATATTTCTGCCGCCGTCCGATAAGTTGTTGTTTTTATTT20.12586532410320958No Hit
ATTTACATCTCTGTACAGCACAAGTGACCCTCAAGTCTGAAATCATAAAC20.12586532410320958No Hit
AGAACTTGTGGCAAAATAATTCATCCGATTTTTAAATTACATCGTGTGTC20.12586532410320958No Hit
TTCCATCAAATTTAATCTAATTCTCTCACGTTTCAATGAAAGTTCCTTTT20.12586532410320958No Hit
GTTGACACTAATGAAGATATTGATTCCTATTTTAAATGTAATCTATCATG20.12586532410320958No Hit
CAATAGTCATGGTAGTTGCCTTTGGTATATTTGAATAGTTAGATTGATTT20.12586532410320958No Hit
CAGGAGATCGTCCTGAGACAGACATCCATCTGCCACTTTCTTGAAGGTTT20.12586532410320958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)