FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139
Sequences flagged as poor quality0
Sequence length39-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG361.1468620579802484Illumina Single End PCR Primer 1 (100% over 50bp)
GGCCTCAGCTATACTTATTGAGAAATTGTGATGACCGGTGTACGTGTATT331.0512902198152279No Hit
TGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC331.0512902198152279TruSeq Adapter, Index 14 (100% over 37bp)
GGGAACCTCAACAGTGTCTTGGTTTTAATAAAATAGTGAAATCTCTTGTT331.0512902198152279No Hit
GAGACATTGCGTTTGGGTAAATCCATGTTGTTTAAATGACATACACGATA321.0194329404268876No Hit
GGGAGTTATTCCACTGTCCACGTACATGAAAGTGTACAAACGTGGAGATA300.9557183816502071No Hit
ACTGAATGTCATCATCTATTAGCAAGTGGTCACCTGCTCAATTACGGTTG300.9557183816502071No Hit
CTGTTATCGAACCAGCTATTGTATGGACGTGTGCCTCGAACAGGAATTCC280.8920038228735266No Hit
CCTGGATGCTGGCTCAACCTCAAATTAGCACTCAGTACACGATCATTTTA270.8601465434851864No Hit
TTCGCGGCGGCCAGCTTCATGGAGAGAGGATAATATAACCAGGACTAAAG270.8601465434851864No Hit
ATTAGGTCATCTGATGTCTTTTATGAAGTCAGAAGGTGATCGAGATTGGA270.8601465434851864No Hit
GGTATCTTCTATTTTGGGGGCTATTAATTTCATTTCTACAACTGTGAATA260.8282892640968461No Hit
GCAAAATCTCACAATATCTCATTTTCAATTATCTTAACTCCCATTCATCT260.8282892640968461No Hit
TGACATTTAAACCGGATAAACAAAAAACATGTTTCGAGTCGCCCTTGTCG260.8282892640968461No Hit
TTTGAAACTTGTAGTTATGTTCAATGACTTTTGGACAGTCAATGGGTCCA250.796431984708506No Hit
TGGTATTGCTGTTTTTCTGACTGCTTGTATTTTAAATAAAAAAGAAAATT250.796431984708506No Hit
ATTATGTCTATCAGTGAATTATGTCTGGTCAATATATGACATTCGATTGC250.796431984708506No Hit
CTCCTGGAGGCGCTGGCTACTTTGTACCAACCATGAATCCAGGAGCAGCT240.7645747053201656No Hit
CCAGCCCGACCGTGTTCGTGCCAACGAAGATCTACTGTACTTTATTAAAT230.7327174259318254No Hit
GGACCATTCCTCTATAATTGTAGGAAAAACCAAAAACAAATCCAATACAT230.7327174259318254No Hit
ATCAGGAAAACTGAAGATAAATCTTCAATTTTCTCACAATGGGGTTTGTC220.7008601465434852No Hit
AATTTATATGGAAAAAATTATTATGTAAATTTTAAAGAAATGGTTAACAT210.669002867155145No Hit
TTGATCCTGACCATAATAGGGTTACATTGAGTAATGGAGATAAGTTAAGT200.6371455877668047No Hit
TCTGCGAAGTCACTTCTGTCTTTCTACATTCCCATTGTATTTTTCTTGTT200.6371455877668047No Hit
GGCAAACCTGCCAAGAAGTAAACCTTGTGATGTTTCAACAAACTTTGGAG190.6052883083784645No Hit
CTTTATCTCACCCCAATACCTTATGAAATATCTTTATCTCACCTATGAAA190.6052883083784645No Hit
TTAAATTTGGTTAGGGGGCAGGTTTTTGCAGGATTGTTAGGGGGTTTTGG190.6052883083784645No Hit
CTGAACAGTATCACTACTGGCTGTATATTGCTCGGAGGTCTGTTTGTGTA180.5734310289901242No Hit
AGCTAAAATAAATTAATGTTAACTAACACCGGGTCAAAGTACGTGGTGAC180.5734310289901242No Hit
AATGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC180.5734310289901242TruSeq Adapter, Index 14 (97% over 40bp)
CGCACGTCTGAGAAACTACCAGCGAGATGGTCCACAATGCGTGACAGACA180.5734310289901242No Hit
CTCGAACAGAAGTGTGCTGTGAAAATACATCTGTAGCAGGCAGTCCATAC180.5734310289901242No Hit
CTTGAATCAACAAATGTCACCAGCACAGGAACAAACCCCTGCACACAATC180.5734310289901242No Hit
CAGGACTTATATTTTTTGCAGATTTTGAAAAGGCATTCGATAGTATAGAT180.5734310289901242No Hit
GCAAAAAGTGTATTTTTTTTTGTTTTGGTTATTACAGAGGTAATCATTTA170.541573749601784No Hit
AGATCACGTCCACGTGAGACTTTTGTACCAAAGCAATTAAAAGCAATCAT170.541573749601784No Hit
GTTAGGGTGGGGTTATCAACCAGAGCGGTTGCAGGCTAGATTTTATATAA170.541573749601784No Hit
AACATCTGTGTGAATTTGCTACATTTAACAACCGTAGGACATTAGAAATA170.541573749601784No Hit
AGACACTCCTGCCCAATGCACCATGGCACCAGATGGAAGACAAGATGTCC170.541573749601784No Hit
GGCACGTGTTTGTAAGGCACACAATGATCTTTTACGGACCTACGAGGCAA170.541573749601784No Hit
CTCAGAGAACACTTGTTTGGCCCTCTGGAATTTTCTCGGCGTGATCTAAT170.541573749601784No Hit
TTGTGTTTAAATTTGGTTAGGGGGCAGGTTTTTGCAGGATTGTTAGGGGG160.5097164702134438No Hit
GCTCAGTCTAACGTTTTGAGGCCACAGAGCTCATTCTGGAGATTTGATGT160.5097164702134438No Hit
GTACGAGACTTGGTTAGAGATGTTACTGGTTTTGCTCCATACGAGAGAAG160.5097164702134438No Hit
CTGAGGGATTAGATATAGGTACCTACTGTATATCGCTATGGACATGACTA160.5097164702134438No Hit
CAGAATTCAGAGAGCTATAAAACATATACTCTTAACTGTTGTCATTGGGC160.5097164702134438No Hit
CTCCTATTAGGGAAGAATCGGGAAGCTCTGGAAAGTAATTAATAGTGATC150.47785919082510353No Hit
CGGCACCAAAAGGGTTAGGGTTGTAATTTTATTAAAATCTGTTCAAGAAT150.47785919082510353No Hit
TACGGGCATTGGAAGACAATGTAGCAAACTTGTATGCAGGATATGGATAT150.47785919082510353No Hit
CGACAGCCACAAGACATAGATACCACAAAAAATCGTAAAACAAAAAAGGT150.47785919082510353No Hit
GTCGTATTCTTTCCTACTCACTCAAATATGTTCTTGCCTGAAATACCATA140.4460019114367633No Hit
TAAAATTTGGAGATTTAATGATATATGATGTATATTTATGTAGTATATTT140.4460019114367633No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC140.4460019114367633No Hit
TGGAAATGATTTTGCCTATATTTTCACTAAAGCATTGTTTGGTGAAGGAG140.4460019114367633No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT140.4460019114367633Illumina Single End PCR Primer 1 (100% over 50bp)
AGTATTGTTTATTTATGCAAGTAGGTATTTAATAGAAAATACCCTAAAAT140.4460019114367633No Hit
CCGCACTGAGGCAGTTGCCTCCACAATATTTCTAAGTTAAAATATGTAAA130.41414463204842306No Hit
CTCGAATATCAATCAATTGTTCATCCTTGCTGCAGGTGACGGAAACAAGC130.41414463204842306No Hit
TATTAATATATAACAATCTTGGGGTGGGAAAATGTACTTTTTCAGAAATG130.41414463204842306No Hit
GCCACGTCCCCGACATTGTACCAGCTGTCCCATTATCTCTTCGATGAATA120.3822873526600828No Hit
CAGCCACCGATATGTAGTTTTGTATGTTGATTTATCACATGAATGCATAA120.3822873526600828No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT120.3822873526600828TruSeq Adapter, Index 14 (97% over 37bp)
GCCGGATCCCCCCGTACCGTTCCTGTTACATTTGGGTCTTACTGCCAGAA110.3504300732717426No Hit
TGAACCTGTCCACATAATACAAGTGCTAGAGGTGATGGCGGCTGCTAGGA100.31857279388340237No Hit
CGTCGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC100.31857279388340237TruSeq Adapter, Index 14 (97% over 40bp)
ATGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC100.31857279388340237TruSeq Adapter, Index 14 (97% over 40bp)
TCGAGGTCTGATTGAGATGCTAATGACGTCATTATAGTAAGATCGAGAAT100.31857279388340237No Hit
GTAAAGGCGGGTCCATCGTGAATAATTCAAGCGTAAATGGCATATGTGCA100.31857279388340237No Hit
CATGTGTCCCTTTTTATACTGGAAACTCTTTGAATTGTTAAACTGTGCTG100.31857279388340237No Hit
TCGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT100.31857279388340237TruSeq Adapter, Index 14 (97% over 37bp)
GTAAATCCTTACGGACAATAACACAAGTCAACTAAAACAATGTGGGTTTT100.31857279388340237No Hit
CTTATCTTATTTTAGTAATGCAGAGGTTGACACATGATCATTATTTTTCT100.31857279388340237No Hit
GTTTTAATGTGTGAGTGTTGGCGTCTTTTGTGGATGTTTTAATGTGTGAG90.2867155144950621No Hit
TCCTCTTGAGGTAGCACTCTGCTACGCTGTCACGAGTGTGTACACAAGTG90.2867155144950621No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTC90.2867155144950621TruSeq Adapter, Index 14 (97% over 36bp)
CGGCGGTGGCAGGTTTGGCAGTGGTGGCTATGGTAATGGTGGAATCGGTG90.2867155144950621No Hit
AAATACGAAACTGTTGTTGGAGAGCGAGGCTTGAAGCTGAGTGGTGGAGA90.2867155144950621No Hit
ATAAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTT90.2867155144950621TruSeq Adapter, Index 14 (100% over 35bp)
CGGAATCACGTACAGTTGTTTGTGTGAATATGGGGGTCCTTTAAACAGTG80.2548582351067219No Hit
AAACGCCAGCATAAGTGTTGAAGGTATAAACCACACGATATATTCAGCAG80.2548582351067219No Hit
CTAATATATACACTGAGTTACCTCCTTGTTTATATATTCTGAATCACTAG80.2548582351067219No Hit
CTCACTTTTTGGGAGAGCGCTACACGAAAGACGGGGACATGGCCGTCATC80.2548582351067219No Hit
TGATAATGGGGAAAATACGCGACATTAAATGTTGAAGTTGCAAGAGCTAA70.22300095571838166No Hit
CTGATGAGCTCTCCTTTATCAAAGGAATTGAAACAGAAGTACAATGTCAG70.22300095571838166No Hit
AAAAAATTAATTTAAGTTGAGAAAGAATATAAAGAACCTCATGACCTAAC70.22300095571838166No Hit
GCAAGAGTGACGTTAGCGAAGGCGATAGAACTGTGTCCAGAGGAGGACCC70.22300095571838166No Hit
GTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT70.22300095571838166TruSeq Adapter, Index 14 (97% over 37bp)
CACTTTTCCGCCAAAGAACGTGCATTGAGAACATTCTCCGCGCATGCGTG70.22300095571838166No Hit
CTGAGGTCCGAGAAAAGTTGGCCCGCATGTACAAAACCACACCAGATGTG70.22300095571838166No Hit
TCGAAATCAAAATAAAATGTGCGACTCATTTTATATATCAAATATGTTGC70.22300095571838166No Hit
TTCAGGTCACCGGGAGCTATAGTACAAAATAATCATGGGAGAACAATGGC60.1911436763300414No Hit
GATGCCTATGATGCCAATGGATATGGGTAAAATGGACGGTAATAACATGA60.1911436763300414No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC60.1911436763300414TruSeq Adapter, Index 14 (97% over 40bp)
TGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT60.1911436763300414TruSeq Adapter, Index 14 (100% over 34bp)
CCTCAGTATGGTCTCAAATCTGTTGTACAGTAGACGGTTTGGTCCTTACT60.1911436763300414No Hit
AGTATATGATGGAATAATATATACTGCAAGTCAAATACTTCTAATATGTT60.1911436763300414No Hit
CGGAATTCAGGAAGCGCAGACGTTGTTCACGCGGGCAGATACTAACGACG60.1911436763300414No Hit
GAGCACGCGGAGAAGTTCATGAAGTACCAGAACAAGAGAGGCGGCCGCGT50.15928639694170119No Hit
AAAACACTAATGTTTGGTGCTCTTTGAACTTTGTACCAAAAGTAGAACCT50.15928639694170119No Hit
TCGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTG50.15928639694170119TruSeq Adapter, Index 14 (96% over 33bp)
CTAGAATGCATCTGTACAATTTGTTAATGAAGCATCATCATTTATATCAG40.12742911755336095No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAAACGTGTGCT40.12742911755336095TruSeq Adapter, Index 18 (96% over 27bp)
TTTTTTTTCGATTTTGGATAAAAAATGGTCATTTTATTTATCATAAAAAA40.12742911755336095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12742911755336095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)