FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068
Sequences flagged as poor quality0
Sequence length39-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAACCTCAACAGTGTCTTGGTTTTAATAAAATAGTGAAATCTCTTGTT331.5957446808510638No Hit
GGCCTCAGCTATACTTATTGAGAAATTGTGATGACCGGTGTACGTGTATT331.5957446808510638No Hit
TGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC331.5957446808510638TruSeq Adapter, Index 14 (100% over 37bp)
GAGACATTGCGTTTGGGTAAATCCATGTTGTTTAAATGACATACACGATA321.5473887814313347No Hit
GGGAGTTATTCCACTGTCCACGTACATGAAAGTGTACAAACGTGGAGATA301.4506769825918762No Hit
ACTGAATGTCATCATCTATTAGCAAGTGGTCACCTGCTCAATTACGGTTG301.4506769825918762No Hit
CTGTTATCGAACCAGCTATTGTATGGACGTGTGCCTCGAACAGGAATTCC281.3539651837524178No Hit
TTCGCGGCGGCCAGCTTCATGGAGAGAGGATAATATAACCAGGACTAAAG271.3056092843326885No Hit
CCTGGATGCTGGCTCAACCTCAAATTAGCACTCAGTACACGATCATTTTA271.3056092843326885No Hit
ATTAGGTCATCTGATGTCTTTTATGAAGTCAGAAGGTGATCGAGATTGGA271.3056092843326885No Hit
GGTATCTTCTATTTTGGGGGCTATTAATTTCATTTCTACAACTGTGAATA261.2572533849129592No Hit
GCAAAATCTCACAATATCTCATTTTCAATTATCTTAACTCCCATTCATCT261.2572533849129592No Hit
TGACATTTAAACCGGATAAACAAAAAACATGTTTCGAGTCGCCCTTGTCG261.2572533849129592No Hit
ATTATGTCTATCAGTGAATTATGTCTGGTCAATATATGACATTCGATTGC251.2088974854932302No Hit
TGGTATTGCTGTTTTTCTGACTGCTTGTATTTTAAATAAAAAAGAAAATT251.2088974854932302No Hit
TTTGAAACTTGTAGTTATGTTCAATGACTTTTGGACAGTCAATGGGTCCA251.2088974854932302No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG251.2088974854932302Illumina Single End PCR Primer 1 (100% over 50bp)
CTCCTGGAGGCGCTGGCTACTTTGTACCAACCATGAATCCAGGAGCAGCT241.160541586073501No Hit
CCAGCCCGACCGTGTTCGTGCCAACGAAGATCTACTGTACTTTATTAAAT231.1121856866537718No Hit
GGACCATTCCTCTATAATTGTAGGAAAAACCAAAAACAAATCCAATACAT231.1121856866537718No Hit
ATCAGGAAAACTGAAGATAAATCTTCAATTTTCTCACAATGGGGTTTGTC221.0638297872340425No Hit
AATTTATATGGAAAAAATTATTATGTAAATTTTAAAGAAATGGTTAACAT211.0154738878143132No Hit
AATGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC200.9671179883945842TruSeq Adapter, Index 14 (97% over 40bp)
TCTGCGAAGTCACTTCTGTCTTTCTACATTCCCATTGTATTTTTCTTGTT200.9671179883945842No Hit
TTGATCCTGACCATAATAGGGTTACATTGAGTAATGGAGATAAGTTAAGT200.9671179883945842No Hit
TTAAATTTGGTTAGGGGGCAGGTTTTTGCAGGATTGTTAGGGGGTTTTGG190.918762088974855No Hit
GGCAAACCTGCCAAGAAGTAAACCTTGTGATGTTTCAACAAACTTTGGAG190.918762088974855No Hit
CTTTATCTCACCCCAATACCTTATGAAATATCTTTATCTCACCTATGAAA190.918762088974855No Hit
CTCGAACAGAAGTGTGCTGTGAAAATACATCTGTAGCAGGCAGTCCATAC180.8704061895551257No Hit
CGCACGTCTGAGAAACTACCAGCGAGATGGTCCACAATGCGTGACAGACA180.8704061895551257No Hit
CTGAACAGTATCACTACTGGCTGTATATTGCTCGGAGGTCTGTTTGTGTA180.8704061895551257No Hit
CTTGAATCAACAAATGTCACCAGCACAGGAACAAACCCCTGCACACAATC180.8704061895551257No Hit
AGCTAAAATAAATTAATGTTAACTAACACCGGGTCAAAGTACGTGGTGAC180.8704061895551257No Hit
CAGGACTTATATTTTTTGCAGATTTTGAAAAGGCATTCGATAGTATAGAT180.8704061895551257No Hit
AGATCACGTCCACGTGAGACTTTTGTACCAAAGCAATTAAAAGCAATCAT170.8220502901353964No Hit
AACATCTGTGTGAATTTGCTACATTTAACAACCGTAGGACATTAGAAATA170.8220502901353964No Hit
GCAAAAAGTGTATTTTTTTTTGTTTTGGTTATTACAGAGGTAATCATTTA170.8220502901353964No Hit
AGACACTCCTGCCCAATGCACCATGGCACCAGATGGAAGACAAGATGTCC170.8220502901353964No Hit
GGCACGTGTTTGTAAGGCACACAATGATCTTTTACGGACCTACGAGGCAA170.8220502901353964No Hit
CTCAGAGAACACTTGTTTGGCCCTCTGGAATTTTCTCGGCGTGATCTAAT170.8220502901353964No Hit
GTTAGGGTGGGGTTATCAACCAGAGCGGTTGCAGGCTAGATTTTATATAA170.8220502901353964No Hit
CAGAATTCAGAGAGCTATAAAACATATACTCTTAACTGTTGTCATTGGGC160.7736943907156674No Hit
TTGTGTTTAAATTTGGTTAGGGGGCAGGTTTTTGCAGGATTGTTAGGGGG160.7736943907156674No Hit
GCTCAGTCTAACGTTTTGAGGCCACAGAGCTCATTCTGGAGATTTGATGT160.7736943907156674No Hit
GTACGAGACTTGGTTAGAGATGTTACTGGTTTTGCTCCATACGAGAGAAG160.7736943907156674No Hit
CTGAGGGATTAGATATAGGTACCTACTGTATATCGCTATGGACATGACTA160.7736943907156674No Hit
CGACAGCCACAAGACATAGATACCACAAAAAATCGTAAAACAAAAAAGGT150.7253384912959381No Hit
CTCCTATTAGGGAAGAATCGGGAAGCTCTGGAAAGTAATTAATAGTGATC150.7253384912959381No Hit
TACGGGCATTGGAAGACAATGTAGCAAACTTGTATGCAGGATATGGATAT150.7253384912959381No Hit
CGGCACCAAAAGGGTTAGGGTTGTAATTTTATTAAAATCTGTTCAAGAAT150.7253384912959381No Hit
GTCGTATTCTTTCCTACTCACTCAAATATGTTCTTGCCTGAAATACCATA140.6769825918762089No Hit
TAAAATTTGGAGATTTAATGATATATGATGTATATTTATGTAGTATATTT140.6769825918762089No Hit
AGTATTGTTTATTTATGCAAGTAGGTATTTAATAGAAAATACCCTAAAAT140.6769825918762089No Hit
TGGAAATGATTTTGCCTATATTTTCACTAAAGCATTGTTTGGTGAAGGAG140.6769825918762089No Hit
CTCGAATATCAATCAATTGTTCATCCTTGCTGCAGGTGACGGAAACAAGC130.6286266924564796No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC130.6286266924564796No Hit
CCGCACTGAGGCAGTTGCCTCCACAATATTTCTAAGTTAAAATATGTAAA130.6286266924564796No Hit
TATTAATATATAACAATCTTGGGGTGGGAAAATGTACTTTTTCAGAAATG130.6286266924564796No Hit
CAGCCACCGATATGTAGTTTTGTATGTTGATTTATCACATGAATGCATAA120.5802707930367506No Hit
GCCACGTCCCCGACATTGTACCAGCTGTCCCATTATCTCTTCGATGAATA120.5802707930367506No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT120.5802707930367506TruSeq Adapter, Index 14 (97% over 37bp)
GCCGGATCCCCCCGTACCGTTCCTGTTACATTTGGGTCTTACTGCCAGAA110.5319148936170213No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT110.5319148936170213Illumina Single End PCR Primer 1 (100% over 50bp)
TCGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT100.4835589941972921TruSeq Adapter, Index 14 (97% over 37bp)
ATGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC100.4835589941972921TruSeq Adapter, Index 14 (97% over 40bp)
GTAAATCCTTACGGACAATAACACAAGTCAACTAAAACAATGTGGGTTTT100.4835589941972921No Hit
TCGAGGTCTGATTGAGATGCTAATGACGTCATTATAGTAAGATCGAGAAT100.4835589941972921No Hit
CTTATCTTATTTTAGTAATGCAGAGGTTGACACATGATCATTATTTTTCT100.4835589941972921No Hit
TGAACCTGTCCACATAATACAAGTGCTAGAGGTGATGGCGGCTGCTAGGA100.4835589941972921No Hit
CGTCGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC100.4835589941972921TruSeq Adapter, Index 14 (97% over 40bp)
CATGTGTCCCTTTTTATACTGGAAACTCTTTGAATTGTTAAACTGTGCTG100.4835589941972921No Hit
GTAAAGGCGGGTCCATCGTGAATAATTCAAGCGTAAATGGCATATGTGCA100.4835589941972921No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTC90.43520309477756286TruSeq Adapter, Index 14 (97% over 36bp)
CGGCGGTGGCAGGTTTGGCAGTGGTGGCTATGGTAATGGTGGAATCGGTG90.43520309477756286No Hit
GTTTTAATGTGTGAGTGTTGGCGTCTTTTGTGGATGTTTTAATGTGTGAG90.43520309477756286No Hit
TCCTCTTGAGGTAGCACTCTGCTACGCTGTCACGAGTGTGTACACAAGTG90.43520309477756286No Hit
AAATACGAAACTGTTGTTGGAGAGCGAGGCTTGAAGCTGAGTGGTGGAGA90.43520309477756286No Hit
ATAAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTT90.43520309477756286TruSeq Adapter, Index 14 (100% over 35bp)
CGGAATCACGTACAGTTGTTTGTGTGAATATGGGGGTCCTTTAAACAGTG80.3868471953578337No Hit
AAACGCCAGCATAAGTGTTGAAGGTATAAACCACACGATATATTCAGCAG80.3868471953578337No Hit
CTAATATATACACTGAGTTACCTCCTTGTTTATATATTCTGAATCACTAG80.3868471953578337No Hit
CTCACTTTTTGGGAGAGCGCTACACGAAAGACGGGGACATGGCCGTCATC80.3868471953578337No Hit
TGATAATGGGGAAAATACGCGACATTAAATGTTGAAGTTGCAAGAGCTAA70.33849129593810445No Hit
CTGAGGTCCGAGAAAAGTTGGCCCGCATGTACAAAACCACACCAGATGTG70.33849129593810445No Hit
TCGAAATCAAAATAAAATGTGCGACTCATTTTATATATCAAATATGTTGC70.33849129593810445No Hit
GCAAGAGTGACGTTAGCGAAGGCGATAGAACTGTGTCCAGAGGAGGACCC70.33849129593810445No Hit
CACTTTTCCGCCAAAGAACGTGCATTGAGAACATTCTCCGCGCATGCGTG70.33849129593810445No Hit
CTGATGAGCTCTCCTTTATCAAAGGAATTGAAACAGAAGTACAATGTCAG70.33849129593810445No Hit
GTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT70.33849129593810445TruSeq Adapter, Index 14 (97% over 37bp)
AAAAAATTAATTTAAGTTGAGAAAGAATATAAAGAACCTCATGACCTAAC70.33849129593810445No Hit
CCTCAGTATGGTCTCAAATCTGTTGTACAGTAGACGGTTTGGTCCTTACT60.2901353965183753No Hit
AGTATATGATGGAATAATATATACTGCAAGTCAAATACTTCTAATATGTT60.2901353965183753No Hit
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC60.2901353965183753TruSeq Adapter, Index 14 (97% over 40bp)
TGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCT60.2901353965183753TruSeq Adapter, Index 14 (100% over 34bp)
CGGAATTCAGGAAGCGCAGACGTTGTTCACGCGGGCAGATACTAACGACG60.2901353965183753No Hit
TTCAGGTCACCGGGAGCTATAGTACAAAATAATCATGGGAGAACAATGGC60.2901353965183753No Hit
AAAACACTAATGTTTGGTGCTCTTTGAACTTTGTACCAAAAGTAGAACCT50.24177949709864605No Hit
GAGCACGCGGAGAAGTTCATGAAGTACCAGAACAAGAGAGGCGGCCGCGT50.24177949709864605No Hit
TCGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTG50.24177949709864605TruSeq Adapter, Index 14 (96% over 33bp)
CGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAAACGTGTGCT40.19342359767891684TruSeq Adapter, Index 18 (96% over 27bp)
GATGCCTATGATGCCAATGGATATGGGTAAAATGGACGGTAATAACATGA40.19342359767891684No Hit
TTTTTTTTCGATTTTGGATAAAAAATGGTCATTTTATTTATCATAAAAAA40.19342359767891684No Hit
CTAGAATGCATCTGTACAATTTGTTAATGAAGCATCATCATTTATATCAG40.19342359767891684No Hit
GCAAAAAGTGTATTTTTTTTTTTTTTTGTTATTACAGAGGTAATCATTTA30.14506769825918764No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14506769825918764No Hit
AAAAAATCTGAAAAACTTTTAAAAACACAAAAAAAGTTCGCGAAACAGTA30.14506769825918764No Hit
GACCAAGCCGCCAGATACATTGGTGCTGGAGCTGCCACCGTTGGTGTAGC30.14506769825918764No Hit
ATTACTCACTATACTCAAACCACATACATCTTAGTTAAAACACAAGATGT30.14506769825918764No Hit
TGGGAACGAGTCTTCACGATGTTATCCGATGATTGATCGGAAACCCGAGA30.14506769825918764No Hit
GAAATACAATTATTTTTTGTGGCCTTATTGCATTTTTATACTTAAATTGT30.14506769825918764No Hit
TCGTCGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC30.14506769825918764TruSeq Adapter, Index 14 (100% over 37bp)
TCTCATTGTTGACGAGTTAACACTACGCCCAGAAGATAGAATACCGTTTG30.14506769825918764No Hit
TCGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGGGTG30.14506769825918764TruSeq Adapter, Index 14 (96% over 29bp)
AATGATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTC30.14506769825918764TruSeq Adapter, Index 14 (97% over 36bp)
GTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTT30.14506769825918764TruSeq Adapter, Index 14 (97% over 38bp)
GTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGT30.14506769825918764TruSeq Adapter, Index 14 (96% over 32bp)
TCGTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTC30.14506769825918764TruSeq Adapter, Index 14 (97% over 36bp)
AAAACACTTAAATGTATCCCCATATATCCCCATATAGCGCTGCGTAGCAT30.14506769825918764No Hit
CCACCGAGATACGGAACTGTGACTGGAGTTCAGACGTGT30.14506769825918764TruSeq Adapter, Index 14 (100% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)