FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139
Sequences flagged as poor quality0
Sequence length39-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCG1173.7273016884358077TruSeq Adapter, Index 14 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGT451.4335775724753106TruSeq Adapter, Index 14 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCA421.33800573431029TruSeq Adapter, Index 14 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCT351.1150047785919084TruSeq Adapter, Index 14 (97% over 37bp)
CAAAAGCACACTTTATTTCCGTTATATTTTACAATATGAATTATGTTCAA321.0194329404268876No Hit
TGAATTTGTCCTAAACGCAGATTTATGGCTTTAATGGCGTTTCAAGAGGT290.9238611022618668No Hit
ATTTCTTGTACATAAACTGTAAGCTGTATCTAATCTTAGATGTTTAAACA270.8601465434851864No Hit
GTGTGGAATGGCATTTGGACACAGCAGGGTCAGTCGTGTCCGGCACTACG270.8601465434851864No Hit
TAAATATTAATCAAACGACGATGTACATTAGTGACATGTTAATGCCAGAA260.8282892640968461No Hit
AGAGCTACAGTCACTATACTTAGTTGCCAGTTCTTCAAATGTTTTCTCCC260.8282892640968461No Hit
TGGCAGTCTGTTTCTTCACTGCGTTCTCCTGAACTCGTCTCAAGAAATCA260.8282892640968461No Hit
TTTGAAATAGCAATCATCTTTGTCATTTTCATCATAAATGACAGATACAC250.796431984708506No Hit
TCCACTTCCACTTACCAGATATAACAACAATCCAGTCACATACATAATCC250.796431984708506No Hit
GTACTGTCTGCCAGCGAGGTCCAGCCCTCATCTGTGGCATCTGAGTTTGG250.796431984708506No Hit
ATTATATGTAAGTTTACGTCGTGGTAAATATGTTCACAGAAATGACACAC250.796431984708506No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT250.796431984708506TruSeq Adapter, Index 14 (97% over 44bp)
GTGTACACCACGGTAGGCCTCCAGGTTGTGACTCTCAATCAGTCGGATGT250.796431984708506No Hit
GCTAGATCGTCATCCAAATATTTCTTAAAAACGGGGAGGGCGAAAGGATT250.796431984708506No Hit
TATAGATCAACATTGAATTACGTTAACATCTATATTAATATATTTATAAA240.7645747053201656No Hit
AAAACCAATACACACATTTTCAATAAAGATCAATTATCACAGAATGAAAA240.7645747053201656No Hit
TTTGCTTCGGAAAATAACTGCAACAAAATCACCAGTGATTGTTTTCTTAA240.7645747053201656No Hit
ATTGTAATAGCCCCCGCCAACACAGGCATAGCAAGCACCAGCAAAAAAGC240.7645747053201656No Hit
CTTGATTCTTATAGACAACAGGTTCAAAGAGAGTAAGTTTTCCCATTTTA240.7645747053201656No Hit
TCCGCTTTCACGACACCCTCCTGGAATTGTTCGTTGACTTTGTCGAACTC230.7327174259318254No Hit
GACAGATCCTAAGATACATGCAAGTATAAAAAAGATAATAGATATAAGGT220.7008601465434852No Hit
GACATGTGTCCGTTATTAACTACACAACTTTGAGCATCGTTATAAAATAA210.669002867155145No Hit
AAGGTACATGATTTTCTGTGGTGCTCCAGCACATTTGACAGGCGTATTTG200.6371455877668047No Hit
ATATTTCATAGGTATTGTGGTGAGATAAGGATATTTCATAGGTATTGTGG200.6371455877668047No Hit
AAAATATGCTGGAAAGCGTGATTCTGCCGTTGACTGTGCGAGTTTCAGAG200.6371455877668047No Hit
CTCTGGACCTCCTCCCTCTGACGACTGTTGTTCAATATCTTTGATTTTCA190.6052883083784645No Hit
AAACTGTTGGGATAGTAGTTTGGTGCATTGCCGTGGTTGTCTGTCACGCA190.6052883083784645No Hit
AAAAACTGTATCAAAGAGAGACCATTACATCAAAATATAATTTTTGAAAT190.6052883083784645No Hit
CTCAACTCCTCAACATACATTGTAATTAAATAAATAAATAAAATTGCTTG190.6052883083784645No Hit
CATTATACAAGTTTACTCATATAGTAGTCCATGGTGACATTAGATACAAG180.5734310289901242No Hit
TTGCTCTTCTTCTTTTGACTAAGATCAAACCTAAGGAGCAGAGCAATGAA180.5734310289901242No Hit
TGAATATATACCTTAAACATATCATCCTATCTGTCCCTCTACTCAACCAA180.5734310289901242No Hit
CGATTCTGGGGACAGAGGACCTCTTAATAACGCAGACTTTGATGATGATG170.541573749601784No Hit
TATTTGTCGGTGAAGTCCTGACCAAGAAGTTGTAACAACGCCTCTTTCCG160.5097164702134438No Hit
TGAAGTTGTTACCCTCATGTTCTTTTTGCTATTTCTAATGTCCTACGGTT160.5097164702134438No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG160.5097164702134438TruSeq Adapter, Index 14 (97% over 43bp)
CTGGACCCTGGCCTAGTGACTGGCCTCCCACGGTACTTTCTAAAGGTTTG160.5097164702134438No Hit
TCTCACTGAAGAAGGTGTTGAAGGAGTCATCACCACCACCAATGGTCTTA160.5097164702134438No Hit
GTTTAAAATAAAGTCTTTTTGTAATAACATATTATAAAGTAAATAGCACA160.5097164702134438No Hit
CTGGCATACGCTATCTGACAGATTATGTCCTTGTTGGTAAAGCGGACAAT160.5097164702134438No Hit
GAATAGTTCCCCCGGTCCATCTGGAGTTGTTTCCAACAGTCCACCACTGA160.5097164702134438No Hit
GACACATAGTCCTGTGGCGTCAAATCTCGAGAAAGAGCCCTATGGCCTCA150.47785919082510353No Hit
CTAACAACAAAATAAATTCTCGTATTTCATATATGCTATATTAATATAAA150.47785919082510353No Hit
CGAACTCACAACGCATTTGTGCCATGACACGACAGAAAATTTGGGCAAAT150.47785919082510353No Hit
CATTAAGGTGCGTGTATAGTATTTTTCTCATATCCATATCCTGCATACAA150.47785919082510353No Hit
CAATCAAACCCCGACACTTAGGGATAATTTATATCAAATATGTACAAATC140.4460019114367633No Hit
CTACAAGATATGGCTTGGTAGCACCACACCCCACTGCCTGTCATCTAGTC140.4460019114367633No Hit
TTTGCACAACTTTCTTGTCGTATCTCCCATCAAACCATGGTTTCCTGTGG140.4460019114367633No Hit
ACAACTTGTTGTCTACAAAGTCCTTTTTTTTAAATATATTTGACCTCTAA140.4460019114367633No Hit
TTCATGTAGAAATTCTTTCTTTTCTTTTTTCATACAACGTGAAATGTCAT140.4460019114367633No Hit
TTTAACTATGGCTCAACTCCTCAACATACATTGTAATTAAATAAATAAAT140.4460019114367633No Hit
TTAACGAAGAAACTCAATTCTGATAAGGATAATATTCAATTAATTAAAAA130.41414463204842306No Hit
TTTTTACAGAACTTCAGTGCTCTTTTGTCCTTGGAAATCCTCAACAATTC130.41414463204842306No Hit
GCCAATACAATCAGTGGACTATGTTAATCGTTTTCGAAAATTAGACTACT130.41414463204842306No Hit
GCGAGAACCATCTTCAAATTTCTTAAGACTTTGGGGTATTTCCGGATTTT130.41414463204842306No Hit
GTTTGGACTTTTTTTTTTCATCTTCCGTTGCACAGGCTTAATTCTGTGTT130.41414463204842306No Hit
CGCAGCGGTTTTTGTCATAGGCATTTTTTTCGGAATTAGCAACAGGGTGT120.3822873526600828No Hit
CCAACATTTTCATATTTCGGGAATACCTTTTCCCAGAATGCAATGAATTC120.3822873526600828No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC120.3822873526600828No Hit
ATACTCTTCATTTCTATTTTTTGAAAACCTAATTATAATGGCAAATTGAT110.3504300732717426No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG110.3504300732717426TruSeq Adapter, Index 14 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCATT110.3504300732717426TruSeq Adapter, Index 14 (97% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACG110.3504300732717426TruSeq Adapter, Index 14 (97% over 40bp)
TTACTCATTATTAAGTCATTATTATAATCATTGTTATTAATATTAAACAA110.3504300732717426No Hit
AAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTC110.3504300732717426TruSeq Adapter, Index 14 (97% over 38bp)
TGCACTTTTAGGATTTTCGATCTAAATACTTCTTTATTCTCGATCTTACT100.31857279388340237No Hit
TTTAACGACTGGAAATCCTTTATCTATGTAGCCTTGAACCAGCTCCGTCT100.31857279388340237No Hit
CCGCACACACGCATGTAATGCACACACACACACACACACACATGCACACA100.31857279388340237No Hit
CAACCAACAGTTTCACAATAGACAGACAGACTGACAGACAGACATCAACT90.2867155144950621No Hit
TGAACTTTCCTCCTCCTTGACTGGTCGATCACCAATTTGTGAGAAATATG90.2867155144950621No Hit
GTCATGTGTCAACCTCTGCACTACTAAAATAACATGAGAAAAATAATGAT90.2867155144950621No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCAT90.2867155144950621TruSeq Adapter, Index 14 (97% over 40bp)
TTTTACTGTAGTCATGATTAAACGGAAGCAAATAGTTTTCAAAGGTTTTT90.2867155144950621No Hit
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTT80.2548582351067219TruSeq Adapter, Index 14 (97% over 36bp)
GAATCCTTAACTGGATCCTACTCATATAAAGCTATCACTTCATGTATGTA80.2548582351067219No Hit
GGACTTGATGCGCTGATGTGGTAAGTTCCGGGAGCCAGCAGTCTCCAGTA80.2548582351067219No Hit
TGAGATTGCACCAAAGTTTTATTTTAAACAAATATTGTCAATGTAAAATA80.2548582351067219No Hit
ATAGTCCTACTTCCCTTTCTGAAATGCACAGTCGTCCAAGCTAAGAGCTC80.2548582351067219No Hit
ATAGCCACCACTGCCGTAATAGCCACCACCGATTCCACCATTACCAAAGC80.2548582351067219No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA70.22300095571838166TruSeq Adapter, Index 14 (97% over 37bp)
CACACACACACACATATGTGACCGGACTGAAAATCCGCCACTGTATTCAC70.22300095571838166No Hit
TCAACTGTTCCATAGCCTGCGAAGTATTCCTGTAATGTTTCTACAGTGGT70.22300095571838166No Hit
GTGGATGCCTACATTGACTGAAGCGCCGTTGGCTTTTTCTCTCTGTATTC70.22300095571838166No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG70.22300095571838166TruSeq Adapter, Index 14 (97% over 40bp)
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG70.22300095571838166TruSeq Adapter, Index 14 (97% over 36bp)
CGTTAATTGGCATCATGTTCTTACCGCCCATTTTACCCATATCCATTGGC70.22300095571838166No Hit
ATAAACAAGTGCAAATCCTGTGGTTTTTCCACCTCCAAAGTTTGTGTGGA60.1911436763300414No Hit
TGATCCTTCTGCATCGTCCTGGGTACCTCCTGGTGACACAGAATCATCGC60.1911436763300414No Hit
CAAAATCCTCTGTGATCATAGGACAGCCAATCAAATCCAAACTGGCAATA60.1911436763300414No Hit
GTTTATGTATAGGTTTTTGTACTCGCTGTTCGACGTTGGTGTGTTTGTAT60.1911436763300414No Hit
GGAACACAAGATTTCTGCTGATGACACTGCAGTACAAATGTCTATATGAC60.1911436763300414No Hit
GGCTACGACATCTGGTACGTTGATTGTGAAGTTAGATGGTTCTTTGGTGA60.1911436763300414No Hit
ATGAATGATTGTGGACAGACGATGGGCCACAATTATCGTTGTTCTATTCT60.1911436763300414No Hit
AAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTTAT50.15928639694170119TruSeq Adapter, Index 14 (97% over 38bp)
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGAC50.15928639694170119TruSeq Adapter, Index 14 (97% over 37bp)
AGAGTACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCT50.15928639694170119TruSeq Adapter, Index 14 (97% over 34bp)
TGTATGCTCATAGTAAATATTGATTTGTGACCAGGGTTATAAGTGATTTC50.15928639694170119No Hit
CTCGTCTTGGTCCGGCTTCTGGATGGGCTGCAGAACGACGCGGCCGCCTC50.15928639694170119No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTCA50.15928639694170119TruSeq Adapter, Index 14 (97% over 40bp)
CACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCT50.15928639694170119TruSeq Adapter, Index 14 (96% over 31bp)
AGTATATGATGGAATAATATATACTGCAAGTCAAATACTTCTAATATGTT50.15928639694170119No Hit
TGAAAAATGATCAATTTTTTGCGAAATTACTGCAAATATATAGAGACATG50.15928639694170119No Hit
TGGGATTCCGTTTTGATGTACCAGTCCTGATATGATGGATCTGAAATTCT50.15928639694170119No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCA50.15928639694170119TruSeq Adapter, Index 14 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA40.12742911755336095TruSeq Adapter, Index 14 (97% over 40bp)
TGGCCGTCTGTTTCTTCACTGCGTTCTCCTGAACTCGTCTCAAGAAATCA40.12742911755336095No Hit
AATGAAGCTTAGATCGAGTACATAATGTTTTGTATTGAACCATAGTGGAG40.12742911755336095No Hit
AGCACACGTTTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTC40.12742911755336095TruSeq Adapter, Index 14 (96% over 32bp)
AAAATCTGCTGGAAAGCGTGATTCTGCCGTTGACTGTGCGAGTTTCAGAG40.12742911755336095No Hit
TAACTTTGAACGACATCTACAGGTTTAAAATGATTACAAGTATTAGTGTG40.12742911755336095No Hit
GAAGCGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGT40.12742911755336095TruSeq Adapter, Index 14 (97% over 36bp)
CACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA40.12742911755336095TruSeq Adapter, Index 14 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)