FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-8_S60_L008_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068
Sequences flagged as poor quality0
Sequence length39-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCA331.5957446808510638TruSeq Adapter, Index 14 (97% over 40bp)
CAAAAGCACACTTTATTTCCGTTATATTTTACAATATGAATTATGTTCAA321.5473887814313347No Hit
TGAATTTGTCCTAAACGCAGATTTATGGCTTTAATGGCGTTTCAAGAGGT291.402321083172147No Hit
ATTTCTTGTACATAAACTGTAAGCTGTATCTAATCTTAGATGTTTAAACA271.3056092843326885No Hit
GTGTGGAATGGCATTTGGACACAGCAGGGTCAGTCGTGTCCGGCACTACG271.3056092843326885No Hit
TAAATATTAATCAAACGACGATGTACATTAGTGACATGTTAATGCCAGAA261.2572533849129592No Hit
TGGCAGTCTGTTTCTTCACTGCGTTCTCCTGAACTCGTCTCAAGAAATCA261.2572533849129592No Hit
AGAGCTACAGTCACTATACTTAGTTGCCAGTTCTTCAAATGTTTTCTCCC261.2572533849129592No Hit
TTTGAAATAGCAATCATCTTTGTCATTTTCATCATAAATGACAGATACAC251.2088974854932302No Hit
ATTATATGTAAGTTTACGTCGTGGTAAATATGTTCACAGAAATGACACAC251.2088974854932302No Hit
TCCACTTCCACTTACCAGATATAACAACAATCCAGTCACATACATAATCC251.2088974854932302No Hit
GTACTGTCTGCCAGCGAGGTCCAGCCCTCATCTGTGGCATCTGAGTTTGG251.2088974854932302No Hit
GTGTACACCACGGTAGGCCTCCAGGTTGTGACTCTCAATCAGTCGGATGT251.2088974854932302No Hit
TATAGATCAACATTGAATTACGTTAACATCTATATTAATATATTTATAAA241.160541586073501No Hit
AAAACCAATACACACATTTTCAATAAAGATCAATTATCACAGAATGAAAA241.160541586073501No Hit
TTTGCTTCGGAAAATAACTGCAACAAAATCACCAGTGATTGTTTTCTTAA241.160541586073501No Hit
CTTGATTCTTATAGACAACAGGTTCAAAGAGAGTAAGTTTTCCCATTTTA241.160541586073501No Hit
ATTGTAATAGCCCCCGCCAACACAGGCATAGCAAGCACCAGCAAAAAAGC241.160541586073501No Hit
TCCGCTTTCACGACACCCTCCTGGAATTGTTCGTTGACTTTGTCGAACTC231.1121856866537718No Hit
GCTAGATCGTCATCCAAATATTTCTTAAAAACGGGGAGGGCGAAAGGATT231.1121856866537718No Hit
GACAGATCCTAAGATACATGCAAGTATAAAAAAGATAATAGATATAAGGT221.0638297872340425No Hit
GACATGTGTCCGTTATTAACTACACAACTTTGAGCATCGTTATAAAATAA211.0154738878143132No Hit
ATATTTCATAGGTATTGTGGTGAGATAAGGATATTTCATAGGTATTGTGG200.9671179883945842No Hit
AAGGTACATGATTTTCTGTGGTGCTCCAGCACATTTGACAGGCGTATTTG200.9671179883945842No Hit
CTCAACTCCTCAACATACATTGTAATTAAATAAATAAATAAAATTGCTTG190.918762088974855No Hit
CTCTGGACCTCCTCCCTCTGACGACTGTTGTTCAATATCTTTGATTTTCA190.918762088974855No Hit
AAACTGTTGGGATAGTAGTTTGGTGCATTGCCGTGGTTGTCTGTCACGCA190.918762088974855No Hit
AAAAACTGTATCAAAGAGAGACCATTACATCAAAATATAATTTTTGAAAT190.918762088974855No Hit
CATTATACAAGTTTACTCATATAGTAGTCCATGGTGACATTAGATACAAG180.8704061895551257No Hit
TTGCTCTTCTTCTTTTGACTAAGATCAAACCTAAGGAGCAGAGCAATGAA180.8704061895551257No Hit
TGAATATATACCTTAAACATATCATCCTATCTGTCCCTCTACTCAACCAA180.8704061895551257No Hit
CGATTCTGGGGACAGAGGACCTCTTAATAACGCAGACTTTGATGATGATG170.8220502901353964No Hit
TATTTGTCGGTGAAGTCCTGACCAAGAAGTTGTAACAACGCCTCTTTCCG160.7736943907156674No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT160.7736943907156674TruSeq Adapter, Index 14 (97% over 44bp)
TGAAGTTGTTACCCTCATGTTCTTTTTGCTATTTCTAATGTCCTACGGTT160.7736943907156674No Hit
TCTCACTGAAGAAGGTGTTGAAGGAGTCATCACCACCACCAATGGTCTTA160.7736943907156674No Hit
GTTTAAAATAAAGTCTTTTTGTAATAACATATTATAAAGTAAATAGCACA160.7736943907156674No Hit
CTGGCATACGCTATCTGACAGATTATGTCCTTGTTGGTAAAGCGGACAAT160.7736943907156674No Hit
GAATAGTTCCCCCGGTCCATCTGGAGTTGTTTCCAACAGTCCACCACTGA160.7736943907156674No Hit
CTGGACCCTGGCCTAGTGACTGGCCTCCCACGGTACTTTCTAAAGGTTTG160.7736943907156674No Hit
CTAACAACAAAATAAATTCTCGTATTTCATATATGCTATATTAATATAAA150.7253384912959381No Hit
CGAACTCACAACGCATTTGTGCCATGACACGACAGAAAATTTGGGCAAAT150.7253384912959381No Hit
CATTAAGGTGCGTGTATAGTATTTTTCTCATATCCATATCCTGCATACAA150.7253384912959381No Hit
GACACATAGTCCTGTGGCGTCAAATCTCGAGAAAGAGCCCTATGGCCTCA150.7253384912959381No Hit
ACAACTTGTTGTCTACAAAGTCCTTTTTTTTAAATATATTTGACCTCTAA140.6769825918762089No Hit
TTCATGTAGAAATTCTTTCTTTTCTTTTTTCATACAACGTGAAATGTCAT140.6769825918762089No Hit
CAATCAAACCCCGACACTTAGGGATAATTTATATCAAATATGTACAAATC140.6769825918762089No Hit
TTTAACTATGGCTCAACTCCTCAACATACATTGTAATTAAATAAATAAAT140.6769825918762089No Hit
CTACAAGATATGGCTTGGTAGCACCACACCCCACTGCCTGTCATCTAGTC140.6769825918762089No Hit
TTTGCACAACTTTCTTGTCGTATCTCCCATCAAACCATGGTTTCCTGTGG140.6769825918762089No Hit
TTAACGAAGAAACTCAATTCTGATAAGGATAATATTCAATTAATTAAAAA130.6286266924564796No Hit
GCGAGAACCATCTTCAAATTTCTTAAGACTTTGGGGTATTTCCGGATTTT130.6286266924564796No Hit
GTTTGGACTTTTTTTTTTCATCTTCCGTTGCACAGGCTTAATTCTGTGTT130.6286266924564796No Hit
TTTTTACAGAACTTCAGTGCTCTTTTGTCCTTGGAAATCCTCAACAATTC130.6286266924564796No Hit
GCCAATACAATCAGTGGACTATGTTAATCGTTTTCGAAAATTAGACTACT130.6286266924564796No Hit
CCAACATTTTCATATTTCGGGAATACCTTTTCCCAGAATGCAATGAATTC120.5802707930367506No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG120.5802707930367506TruSeq Adapter, Index 14 (97% over 43bp)
ATACTCTTCATTTCTATTTTTTGAAAACCTAATTATAATGGCAAATTGAT110.5319148936170213No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG110.5319148936170213TruSeq Adapter, Index 14 (97% over 37bp)
TTACTCATTATTAAGTCATTATTATAATCATTGTTATTAATATTAAACAA110.5319148936170213No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCATT110.5319148936170213TruSeq Adapter, Index 14 (97% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACG110.5319148936170213TruSeq Adapter, Index 14 (97% over 40bp)
TGCACTTTTAGGATTTTCGATCTAAATACTTCTTTATTCTCGATCTTACT100.4835589941972921No Hit
CCGCACACACGCATGTAATGCACACACACACACACACACACATGCACACA100.4835589941972921No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCG100.4835589941972921TruSeq Adapter, Index 14 (97% over 40bp)
TTTAACGACTGGAAATCCTTTATCTATGTAGCCTTGAACCAGCTCCGTCT100.4835589941972921No Hit
CGCAGCGGTTTTTGTCATAGGCATTTTTTTCGGAATTAGCAACAGGGTGT90.43520309477756286No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCAT90.43520309477756286TruSeq Adapter, Index 14 (97% over 40bp)
CAACCAACAGTTTCACAATAGACAGACAGACTGACAGACAGACATCAACT90.43520309477756286No Hit
TTTTACTGTAGTCATGATTAAACGGAAGCAAATAGTTTTCAAAGGTTTTT90.43520309477756286No Hit
TGAACTTTCCTCCTCCTTGACTGGTCGATCACCAATTTGTGAGAAATATG90.43520309477756286No Hit
GTCATGTGTCAACCTCTGCACTACTAAAATAACATGAGAAAAATAATGAT90.43520309477756286No Hit
ATAGTCCTACTTCCCTTTCTGAAATGCACAGTCGTCCAAGCTAAGAGCTC80.3868471953578337No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC80.3868471953578337No Hit
GAATCCTTAACTGGATCCTACTCATATAAAGCTATCACTTCATGTATGTA80.3868471953578337No Hit
ATAGCCACCACTGCCGTAATAGCCACCACCGATTCCACCATTACCAAAGC80.3868471953578337No Hit
GGACTTGATGCGCTGATGTGGTAAGTTCCGGGAGCCAGCAGTCTCCAGTA80.3868471953578337No Hit
TGAGATTGCACCAAAGTTTTATTTTAAACAAATATTGTCAATGTAAAATA80.3868471953578337No Hit
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG70.33849129593810445TruSeq Adapter, Index 14 (97% over 36bp)
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA70.33849129593810445TruSeq Adapter, Index 14 (97% over 37bp)
CACACACACACACATATGTGACCGGACTGAAAATCCGCCACTGTATTCAC70.33849129593810445No Hit
TCAACTGTTCCATAGCCTGCGAAGTATTCCTGTAATGTTTCTACAGTGGT70.33849129593810445No Hit
GTGGATGCCTACATTGACTGAAGCGCCGTTGGCTTTTTCTCTCTGTATTC70.33849129593810445No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG70.33849129593810445TruSeq Adapter, Index 14 (97% over 40bp)
ATAAACAAGTGCAAATCCTGTGGTTTTTCCACCTCCAAAGTTTGTGTGGA60.2901353965183753No Hit
GGAACACAAGATTTCTGCTGATGACACTGCAGTACAAATGTCTATATGAC60.2901353965183753No Hit
GGCTACGACATCTGGTACGTTGATTGTGAAGTTAGATGGTTCTTTGGTGA60.2901353965183753No Hit
TGATCCTTCTGCATCGTCCTGGGTACCTCCTGGTGACACAGAATCATCGC60.2901353965183753No Hit
CAAAATCCTCTGTGATCATAGGACAGCCAATCAAATCCAAACTGGCAATA60.2901353965183753No Hit
ATGAATGATTGTGGACAGACGATGGGCCACAATTATCGTTGTTCTATTCT60.2901353965183753No Hit
GTTTATGTATAGGTTTTTGTACTCGCTGTTCGACGTTGGTGTGTTTGTAT60.2901353965183753No Hit
TGTATGCTCATAGTAAATATTGATTTGTGACCAGGGTTATAAGTGATTTC50.24177949709864605No Hit
CTCGTCTTGGTCCGGCTTCTGGATGGGCTGCAGAACGACGCGGCCGCCTC50.24177949709864605No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTCA50.24177949709864605TruSeq Adapter, Index 14 (97% over 40bp)
AGTATATGATGGAATAATATATACTGCAAGTCAAATACTTCTAATATGTT50.24177949709864605No Hit
TGAAAAATGATCAATTTTTTGCGAAATTACTGCAAATATATAGAGACATG50.24177949709864605No Hit
AAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTTAT50.24177949709864605TruSeq Adapter, Index 14 (97% over 38bp)
TGGGATTCCGTTTTGATGTACCAGTCCTGATATGATGGATCTGAAATTCT50.24177949709864605No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATCA50.24177949709864605TruSeq Adapter, Index 14 (97% over 37bp)
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGAC50.24177949709864605TruSeq Adapter, Index 14 (97% over 37bp)
CGTTAATTGGCATCATGTTCTTACCGCCCATTTTACCCATATCCATTGGC40.19342359767891684No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA40.19342359767891684TruSeq Adapter, Index 14 (97% over 40bp)
TAACTTTGAACGACATCTACAGGTTTAAAATGATTACAAGTATTAGTGTG40.19342359767891684No Hit
AATGAAGCTTAGATCGAGTACATAATGTTTTGTATTGAACCATAGTGGAG40.19342359767891684No Hit
CACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA40.19342359767891684TruSeq Adapter, Index 14 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACGA30.14506769825918764TruSeq Adapter, Index 14 (97% over 36bp)
TTTGCAATAGCAATCATCTTTGTCATTTTCATCATAAATGACAGATACAC30.14506769825918764No Hit
AAGTCTTCCTGAGTAGAACTAGTAGTGAGCGGAAACAGTGCCTTGCTCAA30.14506769825918764No Hit
TGGCCGTCTGTTTCTTCACTGCGTTCTCCTGAACTCGTCTCAAGAAATCA30.14506769825918764No Hit
CGTTAATTGGCATCATGTTCTTACCGCCCATTTTACCCATATCCATTGGCATCATAGGCATC30.14506769825918764No Hit
TGAAGATACACTTACAACTTAGAGATTTAAACGTCTAATTGCATAACCTC30.14506769825918764No Hit
ACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGTGG30.14506769825918764TruSeq Adapter, Index 14 (96% over 32bp)
ATAAGACAGAAAAGACCCATAGCTTCAGCCAAGGCAAATCCTAGAATAGC30.14506769825918764No Hit
TGTTTACAGAACTTCAGTGCTCTTTTGTCCTTGGAAATCCTCAACAATTC30.14506769825918764No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACGA30.14506769825918764TruSeq Adapter, Index 14 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGAC30.14506769825918764TruSeq Adapter, Index 14 (97% over 38bp)
GTTGCAGAAATTACAACATTCCAAGTATGAGCCAAAGGTAGACAGTTCTT30.14506769825918764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)