FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72390
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2450.3384445365381959No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.3121978173780909No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1300.17958281530598147Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN920.1270893769857715No Hit
CTTCTATCGTGTACGATCTTCCTGCGCCCGTGAATATTTCTGCTATGTGA860.11880093935626468No Hit
CGTGTCTTCTATCGTGTACGATCTTCCTGCGCCCGTGAATATTTCTGCTA760.10498687664041995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)