FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72390
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.45586406962287607No Hit
GCCAATTCCACCCTAGGCGTATAATGTATTACTAAAAAAAACCCCCTTAC2140.29562094211907725No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2090.2887139107611548No Hit
GAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAA1640.22655062853985355RNA PCR Primer, Index 36 (96% over 30bp)
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1180.1630059400469678No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1010.13952203343003178No Hit
CTCAAATCCAAGCTACGATGTCAGGCCCAAAATATGGAACAGCTCTAAGT800.11051250172675785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)