FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-7_S52_L007_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58683
Sequences flagged as poor quality0
Sequence length31-151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATTCCACCCTAGGCGTATAATGTATTACTAAAAAAAACCCCCTTAC1880.3203653528279059No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.3203653528279059No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1280.21812109128708487No Hit
CTCAAATCCAAGCTACGATGTCAGGCCCAAAATATGGAACAGCTCTAAGT790.13462161102874767No Hit
ACGATATCTGCCCCCAAGGAAGCACATAGCCTAAAAAAGCAGCTATTATT710.12098904282330487No Hit
TTTTAATCCAACTTTAATTATTTACCTTTTCTGACACCCCTAATAATCAA690.11758090077194418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)