FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-6_S44_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-6_S44_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272
Sequences flagged as poor quality0
Sequence length47-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG301.3204225352112675Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC190.8362676056338028No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT150.6602112676056338Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGCTCGTCTTGACCACAAGTTCGACTTGATGTACGCCAAGAGAGCTTT90.39612676056338025No Hit
TCCGGATAGAATTATGATTATATCAAAATTTCAGTTAAAGGAAATTCCGA90.39612676056338025No Hit
CACATCACTGTTTACTAGTTGTTGATGTACAATCAATTATCTACAAAGGA80.35211267605633806No Hit
AAACTGAAACTGTTGAGGCCAACAATGACGATATGCCACCACTAGAGGGA80.35211267605633806No Hit
GGTTACAGTAGTTCTTGGTGGGTATTTTTCAGTTCTTCAGTTGGAAGAGT80.35211267605633806No Hit
GGCGTGGTCCAGTGGTAGAACATCGAACCAAGGATTGGAGGTCGTGGGTT80.35211267605633806No Hit
TTCCATGGCATGGACCTCACCCGTGACAAGCTCTGCTCCATGGTAAAGAA80.35211267605633806No Hit
AAATCAGAATAGAGATAATATCACAAACTTATTGCAGTTTTCTGAAAATC80.35211267605633806No Hit
TTTCAATATTGCGCGAAATAAATATTAACTGTACACCACCATTGTTATTA70.30809859154929575No Hit
TAGAAAATAATTATTTGATAGTTATTTTAACGTTGTATACAAGCATTTAG70.30809859154929575No Hit
CGAGCTTATTTTAGTGCGGCAACTATGATTATTGCTATCCCTACAGGAAT70.30809859154929575No Hit
TTATACTTCAACTCTCTGATATAATTTTCATTAATCTGACCTCAATACGT70.30809859154929575No Hit
GGCGGAGATGGCGGACCACTCTAACTTGATACGTAGTCTTGTTGTGAGAG60.2640845070422535No Hit
ATGAGCAATGGAATCAGAATGTGGACTTATTTGTGTTGTGAGCAAGTGAG60.2640845070422535No Hit
GTGATTAGTTACACTGGTGTTAAATTGTTGTTGTTTGTAGTTGGTGCAGT60.2640845070422535No Hit
GTCAAGGTCACAGCTTTGTCCGTCCGTCTGCTGCTGTCCGGACCTTATCT60.2640845070422535No Hit
GCCATATTGTGTATTTACAGAAAGTTGCATTTTGGGACATAAGTTTATTT60.2640845070422535No Hit
CAGAGATTAGTCATGGAGTCCTGGGGTGTGTATGTTGGCGCCGACGTTAT60.2640845070422535No Hit
CCAACCAGATGGTGAAATGTGACCCACGTCACGGCAAGTACATGGCCTGC60.2640845070422535No Hit
GTGAAATGCCACATTTTATATCATAATATATAATACTTGGTAGTCTCATT60.2640845070422535No Hit
ATTTACAGCCAGGGTAGTAAATATTTGTTAGTGGAGACTCTCAGCTTACA60.2640845070422535No Hit
GTTACCTGATCGCACTTGTCTTCTACAAATGGGTATCTTACAATGCCTGG60.2640845070422535No Hit
AGCAAACTTGTAAATTGTTCAGTTGCACATGTAATGCACGGTCTGGAGGT60.2640845070422535No Hit
ACTGTATCTATATGATCCAATGGGTTTATAAATCTGTCCTTTATATAGCA50.22007042253521128No Hit
GCCGAATCTGCTTGACGTAAAATATAAACAGAGTTGGTAGTGCGTGAAAA50.22007042253521128No Hit
TTGTTGTGCTTGTGTACTTCAAACGGGAGCCTGAACCTGATGTATTCAAA50.22007042253521128No Hit
TTTAATGTATCAAAGTAAACAAACATACAACAATATTCTACTCAATTTTC50.22007042253521128No Hit
CGGGTATTCACATGTTATATCTCAATTGATGGATGTTAAATACAAAGGTT50.22007042253521128No Hit
AGTGTGGGTAGGAATCCTCCGAATGGCTGTTATGTTCATTTTATCAATTT50.22007042253521128No Hit
CTGTACAAAAATGGCTTAAATTTTTATATTTTATATTTCTTTATTTCCTC50.22007042253521128No Hit
CATCAAATTTGTTTTCGGTGAATAACTGCTGAAAGGGTTTATGTATTTAA50.22007042253521128No Hit
GCTGTTTGTCTTATTTCACACTTAAATTCAGATGAAGTTAATAAAAATTA50.22007042253521128No Hit
GTACAAGTGACCTCTGTTACCTAGCAACTGTCGGGGTATGTTCATCTTTT50.22007042253521128No Hit
CAACAAGTTGGTTTCCCACAGAAAATACAGAATGTGTGGACAACATTAAC50.22007042253521128No Hit
CTGGACACAGTATTAATGACTAAATGTCGTCTGCACGTTTTATTATTAGG50.22007042253521128No Hit
GGTCACCCTGTAATGCGAATGCGTGATTTTGCTCCATGTTGATCTGGATT50.22007042253521128No Hit
CACTCTGGTGATTATGGTTTAAGAATTTATTAATGGAAAAAAATATTTAA50.22007042253521128No Hit
AAGAACTGTTAATCACTGTGGTTAAAATTAGAACAAGTGGATGATGACCT50.22007042253521128No Hit
CTCAAGTACTTCAACAACTTTAACATAAATTTCTGGGAATGCTATTGTTT50.22007042253521128No Hit
TTTTAGGTCATGTTTATCAAACCTGCATCTAAGTTACTAAATTTATAGGT50.22007042253521128No Hit
AATAGATTTTGATAAAAATAGCCTTTAGCAATGCCTATTTTATCATAATT40.17605633802816903No Hit
GGAAAATAAACAACAGAACTTTATACAGGTTATTTGAATATGAACAAGAA40.17605633802816903No Hit
GTGATATTGAAGGTACGATTATGTTTATAGGGCTGATAGGTTTATGAAGG40.17605633802816903No Hit
AAAAAACTGATGAAGTATAAGAGCGGGGTTTTCTCGGATACCCAATGCAG40.17605633802816903No Hit
GTTGTACCAGGAGGTGACTTGGCCAAGGTCCAGAGAGCCGTGTGCATGTT40.17605633802816903No Hit
TCCACTCTGTCAGTTAATAGAAACATAATTTTTTGGGACAATGTATATAC40.17605633802816903No Hit
AAATGAAAGAAAACAAAATATAATGAAATTAAATTTAAATACAGGTACAG40.17605633802816903No Hit
TTTTGCTTCGTTTTTAGCTCGCCGGGACAAAGTCCAAGGAGAGCTATTGT40.17605633802816903No Hit
CAAAACTATTCAAGGTCCTCCTAGCAAAAGAATGCACCGCCCTGCACCAT40.17605633802816903No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17605633802816903No Hit
CAGAAGTAGACTTAGTGACAGATCTAATAATTACAAAAACAGTTATTCCC40.17605633802816903No Hit
CTATCAAGTTGTAAAATTCATGTTTCGAAATCTAGCAATTATTGAGGATA40.17605633802816903No Hit
TCCAGTACTACTGACGTTTCCATCGTGCAATTTTGTCTTATCTCTGCCTT40.17605633802816903No Hit
CCGAAGCCTCAGAATTTACCAAATCTTTCTAACATTATGAAAGCTCAGAT40.17605633802816903No Hit
CAGCAACCTTTGGGTCAGAGTGGGTATGGTGCAGGTTACAGTGGTGATGG40.17605633802816903No Hit
CAAAACGTTAAGCAGGGAATGATTAGATTCGGAAATATGCCACCAGAACA40.17605633802816903No Hit
TGCCATCGGCGGAATTTGAGGAATTTAAGAGCACGGTCCTGGAACGGTTG40.17605633802816903No Hit
CGTCTACTACTACTTTTACTTCAACGACGATCCTTAACATCTGTGTTGGT40.17605633802816903No Hit
GTACTCGCCCAGTCATCATTGCAGCTGCATTTTGGTGTTGTTATCCACTG40.17605633802816903No Hit
CTATGGTTCTGGTGGATTTGGCCAAGATTACCAGGGATCATACGGCGGTG40.17605633802816903No Hit
GATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGC40.17605633802816903TruSeq Adapter, Index 14 (97% over 34bp)
AGACAAGATTCATAACTTGGGGTTGCATTTTGGCAGAGTTATGGCCCTTT40.17605633802816903No Hit
TGACCTTCCTCTATGTGAGGCAGTATGGACGTGAGACTAGTACAGAAGTA40.17605633802816903No Hit
CGAAGACCAATGGAATGAATGGTAACAAGGAAGACACAGGAAGTATTGTG40.17605633802816903No Hit
TTTGTTTTTAAATAAATGTTAAATATCAAACCTAATCGAACCAGCAAATG40.17605633802816903No Hit
GCCACTTTAGCTTTGGCAATTACGGTTAATTCTACTGTATCCCTGTCTAT40.17605633802816903No Hit
GTCTATATTAGAGGTGTCCAAAGGCGTCTATATTAGGGTTGTCCAAAGGC40.17605633802816903No Hit
TACCAATACTTTTTTATATAGTGTACACATTGTACGAACATACAAACTAA40.17605633802816903No Hit
GGAGTCATAAGTCATGCAGATAAAGTCCCTTTTCATAATTATTTTACTAT40.17605633802816903No Hit
CAGCAGTAGCTGCATTGACATTTGTGGCATTGTACACGCATGTTCATACT40.17605633802816903No Hit
AGAGCTCTGACCGTCCCTGAGCTCACCCAACAGATGTTCGACGCCAAGAA40.17605633802816903No Hit
AACTGTCAGATATAGAGGTTCTGAAGGATATACCTGGCTACAAGGCCATT40.17605633802816903No Hit
TATTTTGCTACTTTTCAAAGTTCTATTAATTTTGTGAGTTTGTTGTTGGA30.13204225352112675No Hit
GTTATAACATGGGATACCAGGACTACTATAATTACAATGATTACTATCAT30.13204225352112675No Hit
CACTGCTCTATTTCTGTATTAGTTTATATTTACCACTGAACCTTGGAAAC30.13204225352112675No Hit
CATCACTTGGACAAATCCTATTGGTGGAACTGGTTCGGCTCCAACTGCAA30.13204225352112675No Hit
CCGACATTAGCGATTCCCTTTCGAGAATTCCGAATGAATTATTCAGTCAT30.13204225352112675No Hit
GTTACATTCAAGAGTGAACCCAGTTCTACATATTCTATAATTATATATAA30.13204225352112675No Hit
GTCAGTTACAAAATAGATGGGACACCTGTGCACTACAGCAATAGTAATAA30.13204225352112675No Hit
TTTGGAACATATTGACATTCTAGCACAACACTCAGTTGCTCATTATCATT30.13204225352112675No Hit
TGAACATAAGGATGTTAGAGCAGACGAAAATCCCATAGACACACACAATC30.13204225352112675No Hit
GTCGGCAGCAGAAGACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTC30.13204225352112675TruSeq Adapter, Index 14 (97% over 36bp)
TACAGATTCTGTTAACAATACATATTCTGTTAACAATACAGATTCTGTTA30.13204225352112675No Hit
GTGCGATCTTCTGCCATGGCTATGTGTGGTGTCCATGAACAAGACGCCTT30.13204225352112675No Hit
GTCTGCCAGCGAGGTCCAGCCCTCATCTGTGGCATCTGAGTTTGGGCAAA30.13204225352112675No Hit
AAAACTGATAAGCAAAAACATATGACCTAAATTATCCTTTTAAATAGGGC30.13204225352112675No Hit
CCATTATTAAAATATAGCAACAAATATATTGTTAATGTTGAGACGATGTG30.13204225352112675No Hit
GAATGAAGGTTCTAGCTTCATTTCTTAAGTTCATGTTTGTTTGACGGTTT30.13204225352112675No Hit
AACAAAGAGGACATACCCGGTGATATTATCAAATTGTATATAGCAATTTT30.13204225352112675No Hit
TTTTCACTTACATATTAAATAGATTTAGTCAGGAATCGGCAAATTTGATC30.13204225352112675No Hit
CTTACTGCGAATTGCGTCTCGGCTCAATGCTAGTTCTGCTAACCTATCGG30.13204225352112675No Hit
GTTTGTTTGACTAAAATAGGACTAGTGGTAACTGAATAGTGGTCTCTTGG30.13204225352112675No Hit
GTTTTTCAAATCCGCCATCTTTGAAGCAGTAGCTGCGTAATGTGGCCATC30.13204225352112675No Hit
CCTACACATATACACACACATACCTATATATGCACATACTTATACATCCT30.13204225352112675No Hit
GCTCGCGCTCCGCTTACGCTCTGAGTGCACTCCGTTTGCGTGCAATTGGG30.13204225352112675No Hit
CGTCGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGC30.13204225352112675TruSeq Adapter, Index 14 (97% over 34bp)
GTTGGTTGATTTTTCACAGAGTTATTGCCCTTTGATTCTATTTGTGACCA30.13204225352112675No Hit
TGTGAAAGACATTTTGTAGGCTGTGATGCAATTTAGGTTACTCCGGGGTA30.13204225352112675No Hit
CTTTGATGTATATATTATATCATTAACCGATGAAGGATTTTCTGCGATAT30.13204225352112675No Hit
AATTGATAAACTGCTTCAGATGATATAATCTTGACACAATAAACGTCATG30.13204225352112675No Hit
CGGGTGTTGACCTCCAGTAGGGGTTTTGCCCATTAATGCTTTTGGGGTGC30.13204225352112675No Hit
TTAAACTGTATGAAACAATGGAACCAAATACTGGTACCACGCACATCCAG30.13204225352112675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)