FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-6_S44_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-6_S44_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272
Sequences flagged as poor quality0
Sequence length47-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1014.445422535211268No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT220.9683098591549295TruSeq Adapter, Index 14 (97% over 44bp)
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC140.6161971830985915No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC120.528169014084507No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG110.48415492957746475TruSeq Adapter, Index 14 (97% over 43bp)
CACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTG100.44014084507042256TruSeq Adapter, Index 14 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTT90.39612676056338025TruSeq Adapter, Index 14 (97% over 36bp)
ACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGC90.39612676056338025TruSeq Adapter, Index 14 (96% over 32bp)
CAGGAGGTAACCGTCCGTAGTTTTCACATCAACATGAGCCTCGATCAAAG80.35211267605633806No Hit
AGAAAATGCACCAGTGATGTTCCTTGCAACTTTCTCACAACATCGTAAGC80.35211267605633806No Hit
AAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTC80.35211267605633806TruSeq Adapter, Index 14 (97% over 38bp)
CAGACTTTCTTTTTTCAGATTTCTGATCAAATATTTTATTTTCTACCAAT80.35211267605633806No Hit
TTAACTGTAGTCTGGTATCTACTGGCAACAAGAATTCTACGTTAGGTACA80.35211267605633806No Hit
TTACAGTGATTTGATTTTTTTAATAAAAATAGAACGTTATAGTGAATACA70.30809859154929575No Hit
GCACAATACGTCCTTGACTTTACTTGCCAATATGATATGATTGTGTTACC70.30809859154929575No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCG70.30809859154929575TruSeq Adapter, Index 14 (97% over 40bp)
CAGCCAAATCTTCACGGGCCTCAGAGAACTCACCCTCCTCCATACCCTCT70.30809859154929575No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCT70.30809859154929575TruSeq Adapter, Index 14 (97% over 37bp)
CGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAG70.30809859154929575No Hit
GAAACCCTAAAGCCCAGTACATTCTTGGCCTTAGTTGTACATTCCTTCCT70.30809859154929575No Hit
GAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGT70.30809859154929575TruSeq Adapter, Index 14 (97% over 39bp)
CGCATTCACTACTGCTGTTTTGTACTTCCCAACTCTCATTCTGCCGGCTT60.2640845070422535No Hit
GTACAATGTACTAATCACTACTCAATATTACCGTTAGTAATGAGAGACCT60.2640845070422535No Hit
TAAATATCATTATAGACATACTGTCAAAGATCACATAAACTGTTAACGAT60.2640845070422535No Hit
GCCAAATATTTGAAATTCGAGAGAGATGCACTCTTTACTCATTGATTTGT60.2640845070422535No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2640845070422535No Hit
CATTTTTTAAGCATCCATTATTTCCTAGTGAAGAAAAAGTACTTGGTAAA60.2640845070422535No Hit
CACATCTTATGTGATAAAAACATTTTTTTTATATTTATTTCATTTTCTTT60.2640845070422535No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCTTGTAATCTCGTATGCCG60.2640845070422535Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCTGGACCTTGGCCAAGTCACCTCCTGGTACAACAGTTGGTGGCTGGTAG60.2640845070422535No Hit
GAAGGCTTTGTCAGTCTTAACATCTTTGTACTTCATCAAAAACATATTGA60.2640845070422535No Hit
CTTTACCAAACTGCTAATGAACACTTTGTATCTTTCATTCACTCACACAT60.2640845070422535No Hit
GGAAAAAAATGTTAAAGATTCTAGTTTCGTTTTTTAAATACATGTATCAG60.2640845070422535No Hit
CAACAATTGAACACCAGTGTTACTTATCACTACGTCAACTACAAATAACA60.2640845070422535No Hit
AGTCACACTAACTACATGCTATACTTAAAAAATGTTTCATTAGCCAATTC50.22007042253521128No Hit
TACCTATTTATATTGGAGTGTTAATGTTAAATTTTGTGTATACAGTCTTT50.22007042253521128No Hit
CTTGACCTTTGACAAACAGACACATGTCAGTCGCATGACACATAGTCTTG50.22007042253521128No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCC50.22007042253521128No Hit
GCCAGATATAACTGAGCATATGCACCAGAACAATAAAGTATTAATAAACA50.22007042253521128No Hit
CTTAGATCCAATCTTTTTATATCCATCTCTAACAACAATTGAAAAACAGA50.22007042253521128No Hit
AGTAGAACCACCATGGACAGCGTCCACGCAGTTGAAAAATTTATCTGTGT50.22007042253521128No Hit
TTTTAAAGAGCTGACAAACTGACACACCAGTTTTGTTTGCTTTAGTGAAC50.22007042253521128No Hit
ATTTATAATATTCCTGACATTGTTAACAATAAATTACACAACTACAAGCC50.22007042253521128No Hit
GAAATATTTTGCATTGAGCATGAATGGTCAATGATGGCGGAATGGTTGGA50.22007042253521128No Hit
TTTGGCAGTAAACCTAACAATGTTGTAATCTTAATCTACCGAAGAATGCG50.22007042253521128No Hit
TGCAAACCACAGATACAATATCAAGGTCTCTGCCAAACCAATAAATTTGG50.22007042253521128No Hit
TTTGGACATGCACTCTTGATGTTTATTTCATTTCCTGTCCATGTTAAAAC50.22007042253521128No Hit
CAGGCCCAGACTATATTTTTTTTATATTCATGAATGAATAGTGTGTTACG50.22007042253521128No Hit
CACAACGTCCTTTCCGGACGTTCCCAACGTTGCTTCCATCAAAGAAACAT50.22007042253521128No Hit
GTGAGCTTCTAACACCTCTTTAAAAAGTACTCATAAACCAAATCATTTTA50.22007042253521128No Hit
AAATGATTTGAAATGAGAAATAGTTTTATATAATTTAAACAAACCTTTGT50.22007042253521128No Hit
AGTACACTAGTTTTTAAGTCATATATTACACTTGGCGAGACACAAAACAC50.22007042253521128No Hit
ATTAGTTTCAATTAAACATTGTCTTTGGAATAAATATTCAACCATTTGAA40.17605633802816903No Hit
CCTGTTTAACCCTTTACTTCATATAGATGCCTTTGGAGACCTCTAATATA40.17605633802816903No Hit
TAGTGAAATGTAAATACGATGATGAATGAAAAATTTCATCCGAAAGTTGG40.17605633802816903No Hit
TAAAAAGTCAATTTACATAATAATAGCAATAAAATTAATACTAGCCAATG40.17605633802816903No Hit
TAAACGTCTTACTTATTGTTATTATTCATTTCTTTAATTATTATTGTTAT40.17605633802816903No Hit
CCCGATTCTGTCTCTCAGGTATACTTCAACCTCAGCACATTCTTCGTCAT40.17605633802816903No Hit
CTTCAATTGATTTTACAAGTCTTGTTACAATCTAAATATAATTTTAACAT40.17605633802816903No Hit
AGATCTTCAAGTCCGTGGCAGAGTGGCAGAAGGCGCTGTCCTAATATCAC40.17605633802816903No Hit
TAGACTTTCAGCCTAAAACTTCCTGGTATCTAATGTTACTCTACAAATCA40.17605633802816903No Hit
CGACTATCGACCTCTAGCAGGCGATATACTTCATTCCTTCCTCCTAAATC40.17605633802816903No Hit
GAAAAATTGTTTTATTTTATTATTTAATAATATATCCAATAGCATGTATA40.17605633802816903No Hit
TAGGACCCCAAAAATGTTCAGACCATGTACTTGCTACAAAGCAAGTTACA40.17605633802816903No Hit
AGCAGATTCACCGCCTCCACCGTATGGTTGTGATCTCTGTGCACCATATC40.17605633802816903No Hit
CGAAAATCCAAAGTCAAACCCGCAAGCTACATTAAGTTTAAGATGTCCAT40.17605633802816903No Hit
TGTTTTTGTATTATCCAACATACTGCAGATGACAAAGATCTACTTTTATT40.17605633802816903No Hit
GCCAGACATCTTCAGACCACGTGGTGGGATGTCGCAGACAGCTGTCTTCA40.17605633802816903No Hit
ATTCTGATCAGTTAACTGAGTGAACACCGCCTTAGGGATGGCAGATCATC40.17605633802816903No Hit
GCGGGACCAGGTCAGTGGTTCGGGCTGTATGTGGTGTTCTAGCCAAAGAA40.17605633802816903No Hit
CGTTCTTTGTCTTTTATGTATGCAGATGGATCAAACCTTGGGTTTCTGGA40.17605633802816903No Hit
CTGAAATCTACTTTATCTCCAAACCTTGGTATATTCCTTAAGTCCATGAT40.17605633802816903No Hit
CGCTTACTTGTTTGCCATTCACATCAATGGAGTATACTCCACTGACTGAC40.17605633802816903No Hit
ATAAGAAATACCACTCTGGTTGAATGTGGAGAGGTGTATTCATGGGGTCG40.17605633802816903No Hit
ACAAGCTTTGCAACAGCTGGGTTCACTTTAACCTGGGTCACTGCAGGCAA40.17605633802816903No Hit
GCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATC40.17605633802816903TruSeq Adapter, Index 14 (97% over 34bp)
TTCACCTTCACCCTCGACGGAGTCGACACCGACCTCTTCGTAGTCCTTCT40.17605633802816903No Hit
TTTTTTTTTATGCGAACTAATTATTTTATATATAAATTAATAACAATAAC40.17605633802816903No Hit
CTGACTTGTGTTAAAAATTTCTTAAGGTATGGCTATTTACAAGTGGCGCT40.17605633802816903No Hit
ATATGTCCCATTTTGTTGCTAATAGTGCTGATTTTTTTATGCAGATGGGA30.13204225352112675No Hit
AAAAAAGTAAAGCAAAAAGGTAGAAAACTTACAAGAGTGGGGGGAAATAA30.13204225352112675No Hit
ACTGTATCCTCAAAGCGGGTCTGGTGGAAACTAAAACTTGTTTCTACCAA30.13204225352112675No Hit
CGTTTTTGTCGTATGTTAATACTTTAGCACTTTCATTTTTATCGGAACAG30.13204225352112675No Hit
AATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAA30.13204225352112675Illumina Paired End PCR Primer 2 (96% over 25bp)
GAGGATGACATTTTTCTGGAGTACCAAATTATGATAAAATAGGCATTGCT30.13204225352112675No Hit
GGGATATTATATATAGAATATAAACATCGGTGTCACGTACATGTAACAGA30.13204225352112675No Hit
GTGGTGGCCAAGCCTTTATACGTGGCCCTTGCACAGAGGAAAGAAGATCG30.13204225352112675No Hit
GTCCATTCCCCATCGCTTTCCCCAGCTGTTCTTAACTAGCCAGGCGTCGG30.13204225352112675No Hit
CGTAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCC30.13204225352112675TruSeq Adapter, Index 14 (97% over 38bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCC30.13204225352112675No Hit
CTCATTGACAAATGTAATGTATGTGGAATTGCTAAATACTGGGGGGTTCG30.13204225352112675No Hit
GGACCATCCACTGACCACCGAGTCCGTTTCCAGACGGTCAAAATCTTCAG30.13204225352112675No Hit
TAGACGATTCACTGTGGTTACTATCCGCATCGTGAAAACAGTTTGTCCAT30.13204225352112675No Hit
TTTACCGACTGATTTGGAACAGAATATGTATTGTTAACAGAATCTGTATT30.13204225352112675No Hit
GTTAATGAGTCACAGCGAAGAATGCTCATTTTTCTCTCAGTGAAATTATT30.13204225352112675No Hit
GCATGTCGTCCTCTGTCGAAGAACTCTTCTAAACTATGTTTTATCCATAC30.13204225352112675No Hit
AATTGTTGTACATGACATTTATTGTGTCAAGATTATATCATCTGAAGCAG30.13204225352112675No Hit
CTTCAATTGTGAAACAAGTTCTTACAACTTAGACAAATCATGCCCGAAGT30.13204225352112675No Hit
GGAAGCCCTTTCCAGTGCTGGAGCCAAGATGTGAGAGCGTACCATCGTGA30.13204225352112675No Hit
TGAAACCTTAACATACAAGAAATACGAACACCAACAGTCTACGCAGCATA30.13204225352112675No Hit
CTAAAATTGTTTTCCACCTGCATTTTCATAATTTCCAATCCGATATTATA30.13204225352112675No Hit
CTTAGACTGAGTCAAACCACCGTCCTTCTGCAAGAAACTGGAAACTTTCT30.13204225352112675No Hit
GGAGAAGGTCCATTTCCGCTTCCGGTTCGTCTGTTGCCATGTGTTGCAGA30.13204225352112675No Hit
CCGCCAAGTCGATGACCTGGTCCGACCAAATAGAAGAACAAAGTTTTTTC30.13204225352112675No Hit
GTTCTTATCTCTGTGTGTCATATTGATGATGGTAAAGACACATGCAAAGT30.13204225352112675No Hit
TTTTTTTATATTTTGGATGTTTAATATAACAGCATAACAGCATATGACGG30.13204225352112675No Hit
ATTTTCTGTACACATGAAAGCAAAGGCGTAGCAGCTGGGGCGGGATGACC30.13204225352112675No Hit
TTTTTTCGGACAGAGTCTTCAACATGACAGCTTTCTCCTTAAGTATGGCA30.13204225352112675No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCC30.13204225352112675TruSeq Adapter, Index 14 (97% over 41bp)
AATTGTTATAACCGCCATATGCTCTGCCGTAATTGTCGCCACGGTAATTG30.13204225352112675No Hit
TATGCAATAAAGTCACAGGTCGACCAACTTTAGTCGATAGTCAAATGTTG30.13204225352112675No Hit
GTTTGGTCGTACTTCTGTACTAGTCTCACGTACATACTGTCTCACATAGA30.13204225352112675No Hit
GTACACTTAAAAAATAATAAAACCAAATTCAGGGGATCAAGTCCTTATTT30.13204225352112675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13204225352112675No Hit
GTTTGTGTAAGGATGTATAAGTATGTATATATTTATATATATGTATGTGT30.13204225352112675No Hit
AGCTGTTGTTGAGATGACGCCGAGAGCGATAGAGAACAGCCCACTAGGAG30.13204225352112675No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCC30.13204225352112675No Hit
AGACACACTACCAACTAAGCTATCCAACCCAGGCTCCCTATAGTAGGATG30.13204225352112675No Hit
GAATTTTATTAAATTAGAAATAAAAAATGTTCTCATGCAAAAATACATTA30.13204225352112675No Hit
CCAGAAGTGTGTGTATGATATGTTTAATTAGTACATGTACCAGAAGTGAG30.13204225352112675No Hit
AAAGCGTGCATATCCTTAATTGATGACAACACTTCTCAAAATCGATTAAC30.13204225352112675No Hit
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGAC30.13204225352112675TruSeq Adapter, Index 14 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)