FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-6_S44_L006_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-6_S44_L006_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263
Sequences flagged as poor quality0
Sequence length47-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC352.771179730799683No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT161.2668250197941409TruSeq Adapter, Index 14 (97% over 44bp)
CAGGAGGTAACCGTCCGTAGTTTTCACATCAACATGAGCCTCGATCAAAG80.6334125098970704No Hit
CAGACTTTCTTTTTTCAGATTTCTGATCAAATATTTTATTTTCTACCAAT80.6334125098970704No Hit
TTAACTGTAGTCTGGTATCTACTGGCAACAAGAATTCTACGTTAGGTACA80.6334125098970704No Hit
AGAAAATGCACCAGTGATGTTCCTTGCAACTTTCTCACAACATCGTAAGC80.6334125098970704No Hit
TTACAGTGATTTGATTTTTTTAATAAAAATAGAACGTTATAGTGAATACA70.5542359461599367No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG70.5542359461599367TruSeq Adapter, Index 14 (97% over 43bp)
GCACAATACGTCCTTGACTTTACTTGCCAATATGATATGATTGTGTTACC70.5542359461599367No Hit
CGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAG70.5542359461599367No Hit
GAAACCCTAAAGCCCAGTACATTCTTGGCCTTAGTTGTACATTCCTTCCT70.5542359461599367No Hit
CAGCCAAATCTTCACGGGCCTCAGAGAACTCACCCTCCTCCATACCCTCT70.5542359461599367No Hit
CGCATTCACTACTGCTGTTTTGTACTTCCCAACTCTCATTCTGCCGGCTT60.4750593824228029No Hit
GTACAATGTACTAATCACTACTCAATATTACCGTTAGTAATGAGAGACCT60.4750593824228029No Hit
TAAATATCATTATAGACATACTGTCAAAGATCACATAAACTGTTAACGAT60.4750593824228029No Hit
GCCAAATATTTGAAATTCGAGAGAGATGCACTCTTTACTCATTGATTTGT60.4750593824228029No Hit
GAAGGCTTTGTCAGTCTTAACATCTTTGTACTTCATCAAAAACATATTGA60.4750593824228029No Hit
CTTTACCAAACTGCTAATGAACACTTTGTATCTTTCATTCACTCACACAT60.4750593824228029No Hit
CATTTTTTAAGCATCCATTATTTCCTAGTGAAGAAAAAGTACTTGGTAAA60.4750593824228029No Hit
CACATCTTATGTGATAAAAACATTTTTTTTATATTTATTTCATTTTCTTT60.4750593824228029No Hit
GGAAAAAAATGTTAAAGATTCTAGTTTCGTTTTTTAAATACATGTATCAG60.4750593824228029No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.4750593824228029No Hit
CAACAATTGAACACCAGTGTTACTTATCACTACGTCAACTACAAATAACA60.4750593824228029No Hit
TCTGGACCTTGGCCAAGTCACCTCCTGGTACAACAGTTGGTGGCTGGTAG60.4750593824228029No Hit
TTTGGCAGTAAACCTAACAATGTTGTAATCTTAATCTACCGAAGAATGCG50.395882818685669No Hit
TACCTATTTATATTGGAGTGTTAATGTTAAATTTTGTGTATACAGTCTTT50.395882818685669No Hit
TGCAAACCACAGATACAATATCAAGGTCTCTGCCAAACCAATAAATTTGG50.395882818685669No Hit
CTTGACCTTTGACAAACAGACACATGTCAGTCGCATGACACATAGTCTTG50.395882818685669No Hit
TTTGGACATGCACTCTTGATGTTTATTTCATTTCCTGTCCATGTTAAAAC50.395882818685669No Hit
CAGGCCCAGACTATATTTTTTTTATATTCATGAATGAATAGTGTGTTACG50.395882818685669No Hit
GCCAGATATAACTGAGCATATGCACCAGAACAATAAAGTATTAATAAACA50.395882818685669No Hit
CACAACGTCCTTTCCGGACGTTCCCAACGTTGCTTCCATCAAAGAAACAT50.395882818685669No Hit
CTTAGATCCAATCTTTTTATATCCATCTCTAACAACAATTGAAAAACAGA50.395882818685669No Hit
GTGAGCTTCTAACACCTCTTTAAAAAGTACTCATAAACCAAATCATTTTA50.395882818685669No Hit
AGTAGAACCACCATGGACAGCGTCCACGCAGTTGAAAAATTTATCTGTGT50.395882818685669No Hit
TTTTAAAGAGCTGACAAACTGACACACCAGTTTTGTTTGCTTTAGTGAAC50.395882818685669No Hit
ATTTATAATATTCCTGACATTGTTAACAATAAATTACACAACTACAAGCC50.395882818685669No Hit
AAATGATTTGAAATGAGAAATAGTTTTATATAATTTAAACAAACCTTTGT50.395882818685669No Hit
AGTACACTAGTTTTTAAGTCATATATTACACTTGGCGAGACACAAAACAC50.395882818685669No Hit
ATTAGTTTCAATTAAACATTGTCTTTGGAATAAATATTCAACCATTTGAA40.3167062549485352No Hit
AGTCACACTAACTACATGCTATACTTAAAAAATGTTTCATTAGCCAATTC40.3167062549485352No Hit
CCTGTTTAACCCTTTACTTCATATAGATGCCTTTGGAGACCTCTAATATA40.3167062549485352No Hit
TAGTGAAATGTAAATACGATGATGAATGAAAAATTTCATCCGAAAGTTGG40.3167062549485352No Hit
GCCAGACATCTTCAGACCACGTGGTGGGATGTCGCAGACAGCTGTCTTCA40.3167062549485352No Hit
TAAAAAGTCAATTTACATAATAATAGCAATAAAATTAATACTAGCCAATG40.3167062549485352No Hit
ATTCTGATCAGTTAACTGAGTGAACACCGCCTTAGGGATGGCAGATCATC40.3167062549485352No Hit
GCGGGACCAGGTCAGTGGTTCGGGCTGTATGTGGTGTTCTAGCCAAAGAA40.3167062549485352No Hit
CGTTCTTTGTCTTTTATGTATGCAGATGGATCAAACCTTGGGTTTCTGGA40.3167062549485352No Hit
CTGAAATCTACTTTATCTCCAAACCTTGGTATATTCCTTAAGTCCATGAT40.3167062549485352No Hit
CGCTTACTTGTTTGCCATTCACATCAATGGAGTATACTCCACTGACTGAC40.3167062549485352No Hit
TAAACGTCTTACTTATTGTTATTATTCATTTCTTTAATTATTATTGTTAT40.3167062549485352No Hit
ATAAGAAATACCACTCTGGTTGAATGTGGAGAGGTGTATTCATGGGGTCG40.3167062549485352No Hit
CCCGATTCTGTCTCTCAGGTATACTTCAACCTCAGCACATTCTTCGTCAT40.3167062549485352No Hit
ACAAGCTTTGCAACAGCTGGGTTCACTTTAACCTGGGTCACTGCAGGCAA40.3167062549485352No Hit
CTTCAATTGATTTTACAAGTCTTGTTACAATCTAAATATAATTTTAACAT40.3167062549485352No Hit
AGATCTTCAAGTCCGTGGCAGAGTGGCAGAAGGCGCTGTCCTAATATCAC40.3167062549485352No Hit
TAGACTTTCAGCCTAAAACTTCCTGGTATCTAATGTTACTCTACAAATCA40.3167062549485352No Hit
CGACTATCGACCTCTAGCAGGCGATATACTTCATTCCTTCCTCCTAAATC40.3167062549485352No Hit
GAAAAATTGTTTTATTTTATTATTTAATAATATATCCAATAGCATGTATA40.3167062549485352No Hit
TAGGACCCCAAAAATGTTCAGACCATGTACTTGCTACAAAGCAAGTTACA40.3167062549485352No Hit
TTCACCTTCACCCTCGACGGAGTCGACACCGACCTCTTCGTAGTCCTTCT40.3167062549485352No Hit
AGCAGATTCACCGCCTCCACCGTATGGTTGTGATCTCTGTGCACCATATC40.3167062549485352No Hit
CGAAAATCCAAAGTCAAACCCGCAAGCTACATTAAGTTTAAGATGTCCAT40.3167062549485352No Hit
TGTTTTTGTATTATCCAACATACTGCAGATGACAAAGATCTACTTTTATT40.3167062549485352No Hit
TTTTTTTTTATGCGAACTAATTATTTTATATATAAATTAATAACAATAAC40.3167062549485352No Hit
CTGACTTGTGTTAAAAATTTCTTAAGGTATGGCTATTTACAAGTGGCGCT40.3167062549485352No Hit
ATATGTCCCATTTTGTTGCTAATAGTGCTGATTTTTTTATGCAGATGGGA30.23752969121140144No Hit
GGAGAAGGTCCATTTCCGCTTCCGGTTCGTCTGTTGCCATGTGTTGCAGA30.23752969121140144No Hit
CCGCCAAGTCGATGACCTGGTCCGACCAAATAGAAGAACAAAGTTTTTTC30.23752969121140144No Hit
AAAAAAGTAAAGCAAAAAGGTAGAAAACTTACAAGAGTGGGGGGAAATAA30.23752969121140144No Hit
ACTGTATCCTCAAAGCGGGTCTGGTGGAAACTAAAACTTGTTTCTACCAA30.23752969121140144No Hit
CGTTTTTGTCGTATGTTAATACTTTAGCACTTTCATTTTTATCGGAACAG30.23752969121140144No Hit
GTTCTTATCTCTGTGTGTCATATTGATGATGGTAAAGACACATGCAAAGT30.23752969121140144No Hit
GAGGATGACATTTTTCTGGAGTACCAAATTATGATAAAATAGGCATTGCT30.23752969121140144No Hit
GGGATATTATATATAGAATATAAACATCGGTGTCACGTACATGTAACAGA30.23752969121140144No Hit
TTTTTTTATATTTTGGATGTTTAATATAACAGCATAACAGCATATGACGG30.23752969121140144No Hit
GTGGTGGCCAAGCCTTTATACGTGGCCCTTGCACAGAGGAAAGAAGATCG30.23752969121140144No Hit
ATTTTCTGTACACATGAAAGCAAAGGCGTAGCAGCTGGGGCGGGATGACC30.23752969121140144No Hit
TTTTTTCGGACAGAGTCTTCAACATGACAGCTTTCTCCTTAAGTATGGCA30.23752969121140144No Hit
AATTGTTATAACCGCCATATGCTCTGCCGTAATTGTCGCCACGGTAATTG30.23752969121140144No Hit
TATGCAATAAAGTCACAGGTCGACCAACTTTAGTCGATAGTCAAATGTTG30.23752969121140144No Hit
GTCCATTCCCCATCGCTTTCCCCAGCTGTTCTTAACTAGCCAGGCGTCGG30.23752969121140144No Hit
GTTTGGTCGTACTTCTGTACTAGTCTCACGTACATACTGTCTCACATAGA30.23752969121140144No Hit
CTCATTGACAAATGTAATGTATGTGGAATTGCTAAATACTGGGGGGTTCG30.23752969121140144No Hit
GTACACTTAAAAAATAATAAAACCAAATTCAGGGGATCAAGTCCTTATTT30.23752969121140144No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.23752969121140144No Hit
GCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTATC30.23752969121140144TruSeq Adapter, Index 14 (97% over 34bp)
GGACCATCCACTGACCACCGAGTCCGTTTCCAGACGGTCAAAATCTTCAG30.23752969121140144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23752969121140144No Hit
TAGACGATTCACTGTGGTTACTATCCGCATCGTGAAAACAGTTTGTCCAT30.23752969121140144No Hit
TTTACCGACTGATTTGGAACAGAATATGTATTGTTAACAGAATCTGTATT30.23752969121140144No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.23752969121140144No Hit
GTTAATGAGTCACAGCGAAGAATGCTCATTTTTCTCTCAGTGAAATTATT30.23752969121140144No Hit
GTTTGTGTAAGGATGTATAAGTATGTATATATTTATATATATGTATGTGT30.23752969121140144No Hit
GCATGTCGTCCTCTGTCGAAGAACTCTTCTAAACTATGTTTTATCCATAC30.23752969121140144No Hit
AGCTGTTGTTGAGATGACGCCGAGAGCGATAGAGAACAGCCCACTAGGAG30.23752969121140144No Hit
AATTGTTGTACATGACATTTATTGTGTCAAGATTATATCATCTGAAGCAG30.23752969121140144No Hit
CTTCAATTGTGAAACAAGTTCTTACAACTTAGACAAATCATGCCCGAAGT30.23752969121140144No Hit
GGAAGCCCTTTCCAGTGCTGGAGCCAAGATGTGAGAGCGTACCATCGTGA30.23752969121140144No Hit
TGAAACCTTAACATACAAGAAATACGAACACCAACAGTCTACGCAGCATA30.23752969121140144No Hit
AGACACACTACCAACTAAGCTATCCAACCCAGGCTCCCTATAGTAGGATG30.23752969121140144No Hit
GAATTTTATTAAATTAGAAATAAAAAATGTTCTCATGCAAAAATACATTA30.23752969121140144No Hit
CTAAAATTGTTTTCCACCTGCATTTTCATAATTTCCAATCCGATATTATA30.23752969121140144No Hit
CCAGAAGTGTGTGTATGATATGTTTAATTAGTACATGTACCAGAAGTGAG30.23752969121140144No Hit
AAAGCGTGCATATCCTTAATTGATGACAACACTTCTCAAAATCGATTAAC30.23752969121140144No Hit
GAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGAC30.23752969121140144TruSeq Adapter, Index 14 (97% over 36bp)
CTTAGACTGAGTCAAACCACCGTCCTTCTGCAAGAAACTGGAAACTTTCT30.23752969121140144No Hit
GTCAAGTCCTAGTGATCTGACACGGGATAAATGAGTTCCTAAATTCCTAT20.1583531274742676No Hit
GCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACG20.1583531274742676TruSeq Adapter, Index 14 (97% over 34bp)
ATTCCAATAACATTGAAAAGGCCATTTCAAAACTGACTCTCTGTGCCGAA20.1583531274742676No Hit
CCCATTGTTTTTTAACATATGCAAATGATTTAAACAGGTTATCAACTTTT20.1583531274742676No Hit
GTTTCTATTCATTCATAGTAATTAATCAAAGTTTATCGGAAAGCAATGCT20.1583531274742676No Hit
AAACAGTTAATTTGAAATGACATAAAAAACAATATGGTGCATTGTACACA20.1583531274742676No Hit
TGATAATGATGCATCCTAACAAGTGACATAAAATTCCAACCTGACGTTGT20.1583531274742676No Hit
ATAACTCACAACTACTTGACCAAGAAAACATTCTGAAATCCTATCATTGA20.1583531274742676No Hit
CTTCAAACAGGGTGCATACCTAAGCCCGTCATTAGAGGATACCAATAATG20.1583531274742676No Hit
TTAAATTGTGAACATGCAAAAAGAGACAAAACTCTTGAAATATTACCCGA20.1583531274742676No Hit
GTAGCGGTTGTTGCTACGTCTTGTTCTGGAGCTGACGTTGCCAAGGCACC20.1583531274742676No Hit
TGAAAATATAGGAAATACACCTACTAACTCGTCAATGATAATAAAGTAAA20.1583531274742676No Hit
TACAGATGCTTCTTTTGATAAAAAACATTTCCATGTATATTCAAAAAGCG20.1583531274742676No Hit
GTTCATTTGGAAATACCTTAAATATCTTTGCATTGGTATATCGTATGTCG20.1583531274742676No Hit
AAGCAAACAGCCAACTGTTATACTCAGTTTGTTTACCACAGATACTGTGA20.1583531274742676No Hit
ATTAACGACACTACTTTCTATTATCTTACACGACTTAGAATTCTTTTTTT20.1583531274742676No Hit
GCATACACAGCAGTAAAATAGCAGACACAACAGTATAATAACATACACAA20.1583531274742676No Hit
CCTAGATCTTGTACATGAAACATATTTAAACAGCATTTCATTTAATTGGC20.1583531274742676No Hit
CCTAGCTCTTGTACATGAAACATATTTAAACAGCATTTCATTTAATTGGC20.1583531274742676No Hit
CCTATATAATTTTCTCCTCTGTTATTTTTTTGTTTCATGTGTAAGTTTCT20.1583531274742676No Hit
CTTTTTTACACACAACACAAAACAAAATGTTTGTAAACAAGAAAAAAGTT20.1583531274742676No Hit
CTGAGAGTTGGCATCATAGTAAAGTGTAGAAACTGGATCATAGTAATATC20.1583531274742676No Hit
CATTCCCAACAATCCTTGGTCCCAGCCTTTGATAACTTGTCCTGTTCCTA20.1583531274742676No Hit
TTCTGATGTCCACATCAAGGGTGGTCATATCCTCGCACATGTTGGCAAGG20.1583531274742676No Hit
ATCTTGCGCGATTTTCAAGTATTCCATCATTGCATCTTCCCTTAGCATGC20.1583531274742676No Hit
CAAGGATGTCAACGCCGCCATTGCTACCATCAAGACCAAGAGAACCATCC20.1583531274742676No Hit
CTCGAATACGTCTTCCCTTCTCTCCAAGAACATTCTGTGTTCTGGTGGCC20.1583531274742676No Hit
CTGGAATGTATTGCAGAACCTTGTTATCTCACCAACACTCTACAATTCAT20.1583531274742676No Hit
AAACCTACATAGATGACATACAAAATTCTTTAGTTACCTAGCTAATGATG20.1583531274742676No Hit
CACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTGCCGACG20.1583531274742676TruSeq Adapter, Index 14 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACG20.1583531274742676TruSeq Adapter, Index 14 (97% over 40bp)
CGTATCCCATGTGAGACCTTTTGCCTATACATTCACCAAGGAAAAACCAG20.1583531274742676No Hit
GGGATCCATTCAACAAAGTAGCTGCTGTTCTTGTTCTGCACGTTCAACAG20.1583531274742676No Hit
AATTGCAAATACACATACAAATCAATTATGCACAACTTTTAATTATTTTT20.1583531274742676No Hit
CCTTCTTCTTCACCCTCACCCTCAACAGAGTCGACACCGACCTCTTCGTA20.1583531274742676No Hit
CACAGGTTGACTGTTAACTAAGTAGTAACTAACTACATTTAGAAGAAAAC20.1583531274742676No Hit
AGATTATTGATATACAACATTATCATACGTTTCTCACTGCTGATTCAAGT20.1583531274742676No Hit
AAACACTCACAATAAAGTTTAAACTAAACGTTAATAAGTTAAACATTACT20.1583531274742676No Hit
TCTTGATGCACATGGTGTTCTTTGTTGTCCGTTTTGTTGTTGTTGTTTTT20.1583531274742676No Hit
CCCAGCTCTCCCGATTGGGTCTGTTAAATAGCAATGTTGGGAAATCCCAA20.1583531274742676No Hit
CACTTTTAAAATTCTACTATGCAATAGAAACATCGGGGCGGAGTCATTTT20.1583531274742676No Hit
TGATAAAACTGAAAAGATAAACAATGTGCTCATTGAAATAACAAAGTGCA20.1583531274742676No Hit
CCAAGCTCAAAATGCTTACATCTCTTGATTGGAAGCTGCTTCCTGTATTT20.1583531274742676No Hit
AGTATCCTTTTATGCCACCATGTTTAAAAATAGATCATCAGTTATTATTA20.1583531274742676No Hit
CAATTCTATGCTTTTACCAAAAATCATTCCCATTATTTATTCATATCAAA20.1583531274742676No Hit
GGAACTTGCACTGCCAACATCACCTACTTAGATATGAAATCATATAGCGT20.1583531274742676No Hit
TCTAAATTCACAATTCAAACATGACAATGCTATCAAAATGTTCAAAATAC20.1583531274742676No Hit
CTAAACTCAATCATTAATATATTCCAAATTTCTAATAAGCGTGTACCCAT20.1583531274742676No Hit
TGACAAAGATTTCAAAATATTGGTAGATGATTGTTACAGCCAAGAGGATA20.1583531274742676No Hit
CCGAACTAACCCAACTAGTAAAAATAGAGTGCCAATTAAAACATGCAACC20.1583531274742676No Hit
TGAAGACGCTGTAGGTATAACTGCATATGCTGGGTTATCCACAAGCCGAA20.1583531274742676No Hit
CGTAAACGGTGGATATATATGTTTGTGTTAACAGAGATAAAATCTTTGCC20.1583531274742676No Hit
CGTCTTTTTGAGCTGTAAACAGATATAGTAAGCTCTCTTTGGCCTGGTTC20.1583531274742676No Hit
ATTTTACTATTTTTATTTTTAAGATTTTGATTTTACAAAAGTCTTTGTTA20.1583531274742676No Hit
CTGTACTTCAATTATTAACAATAAACAATTATTTTCAATGTGGAGTTTTT20.1583531274742676No Hit
TGTTACCAGACATCACTACAGATGTCGTCAGCTTGTCAAATAAAAACCAT20.1583531274742676No Hit
TAATAACCTTACATTGGGCCGAGAACAGAGGCTTAATGTCATTTCAATAC20.1583531274742676No Hit
GCTTGGTCGATATCACGCTTTGCTACACTTGTCTGGATACTTCTCATCTG20.1583531274742676No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCTTGTAATCTCGTATGCCG20.1583531274742676Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CTCGTCCCCGTATATGCCATATACATGTATGACATGAACTGTGTTAAACA20.1583531274742676No Hit
CAAATCGTTAATCAAAACCAGGAACTTTTCATCGGCAACCTGTGCATCAG20.1583531274742676No Hit
CTTGTACCTTTCCTCAAATCTTGTCTTAACTTCTCTCTTTGCCTTACGTT20.1583531274742676No Hit
CAGGACTAACTTTTGATGGCTTTGGACTGGGAATTTGTTCTCTTTCAGGA20.1583531274742676No Hit
CGGGGGTTCTGTACAATGGTCATCACTCTCTCAATCTCTTCCTCTGTCAG20.1583531274742676No Hit
TATTTTAACATGTCGTCTGTGCTTATTCATTGTTTAACATGTCGTCTGTG20.1583531274742676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)