FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-3_S20_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-3_S20_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3972
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2646.646525679758309No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1042.6183282980866065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541.3595166163141994No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG90.22658610271903326Illumina Single End PCR Primer 1 (100% over 50bp)
TCTACTTGTACCCAACCATAGTTCTACTTGTACCCAACCATAGTTCTACT90.22658610271903326No Hit
TTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1510574018126888No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT60.1510574018126888Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12588116817724068No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.12588116817724068No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10070493454179255No Hit
NCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.10070493454179255No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCC40.10070493454179255No Hit
GGATAGATTCAGCTTATGTTTAACGGTAATTCTTGTTATTTGTGTAGGGG40.10070493454179255No Hit
TGTGCTTACCAGAAACAACAAACTTCGACAATGAAGTGCAGAACAAACCT40.10070493454179255No Hit
GGCGTTCAAATGAGCCTGACCTATCGGAACAAAATCTTGTAGATTGTGCA40.10070493454179255No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC40.10070493454179255No Hit
GTGAAGCGTGCTGTGCGTTATCTTAAGAACGTTACCGCTAAGAAAGAGGT40.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)