FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-3_S20_L003_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-3_S20_L003_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146
Sequences flagged as poor quality0
Sequence length31-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC25812.022367194780987No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA452.096924510717614No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401.863932898415657No Hit
TCTACTTGTACCCAACCATAGTTCTACTTGTACCCAACCATAGTTCTACT90.4193849021435228No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2329916123019571No Hit
GGCGTTCAAATGAGCCTGACCTATCGGAACAAAATCTTGTAGATTGTGCA40.1863932898415657No Hit
NCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.1863932898415657No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCC40.1863932898415657No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCC40.1863932898415657No Hit
GTGAAGCGTGCTGTGCGTTATCTTAAGAACGTTACCGCTAAGAAAGAGGT40.1863932898415657No Hit
GGATAGATTCAGCTTATGTTTAACGGTAATTCTTGTTATTTGTGTAGGGG40.1863932898415657No Hit
TGTGCTTACCAGAAACAACAAACTTCGACAATGAAGTGCAGAACAAACCT40.1863932898415657No Hit
TTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1863932898415657No Hit
TTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13979496738117428No Hit
CTTAAATCAACCTACCAGTGAATAACTCTAGAGTAGGATTAAAACACATG30.13979496738117428No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.13979496738117428No Hit
CTTGCCACGGCCGTTGCTGTGGTAATTGGTGTGGCGGAGAAAACACAAGG30.13979496738117428No Hit
TTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13979496738117428No Hit
TTTGAAGCTGGTATCTCGAAGAACGGTCAAACCAGAGAACACGCTCTCTT30.13979496738117428No Hit
TTTATAGGCAGTTATTTTGTATACAATTGTAGAGAGTTAATTTTGGATCT30.13979496738117428No Hit
GGGACTGGGACTTATTACACGTGTACGTATGTGATTAAATGTCGTCTGCA30.13979496738117428No Hit
GGTTGTCATGGGTACCGCAGTTTTTTGTTTACCTGCAATGCATTCATCCA30.13979496738117428No Hit
CCCGATTAGCTCAGTGGGTTATGCATCTGACTATCAATCCAGGGATCATG30.13979496738117428No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC30.13979496738117428No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCC30.13979496738117428No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG30.13979496738117428Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCC30.13979496738117428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)