FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-2_S12_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-2_S12_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135
Sequences flagged as poor quality0
Sequence length37-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC591.8819776714513556No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC441.4035087719298245No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.8612440191387559No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG80.2551834130781499TruSeq Adapter, Index 14 (97% over 43bp)
CCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA50.1594896331738437Illumina PCR Primer Index 1 (96% over 28bp)
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.1594896331738437No Hit
CAAACATAAAAAACCTACGGTTTTAGAGCTTAAAGTATGGAAACTTTAGT40.12759170653907495No Hit
AGTTGGGATCCGTGGCGGTAAGTGTTGTGACGGTAGATCCTGAAGCGGAC40.12759170653907495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)