FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC933.3238027162258756No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG361.2866333095067906Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT311.1079342387419584Illumina Single End PCR Primer 1 (100% over 50bp)
TGAAAACTTTTCTGGACAAAATAAAAAGATTCGATTTGATATGGAAGAAT130.46461758398856323No Hit
AAGACTAATGTTTGTTTGATCTGGGTTTTACGAAAAATCGGCTTTATTTT110.39313795568263044No Hit
TTTGAACCGGCTAATCAGATGGTCAAATGTGACCCCCGCATGGGAAAATA110.39313795568263044No Hit
AAGAAATATGACTTGTGGATTATTCCAGTTTCTTTGATATTTGAACAGTA100.35739814152966404No Hit
GTTTGTTCAAACTGCAGTGATTCGTCAAAAAACATGGCGGCCAGGGGGTG90.32165832737669764No Hit
ACCGGATAACGATTTCGTCGTCGTGATTTAAAATGTTTTCATCACCAGAA80.28591851322373124No Hit
TGTTGATGTGTTTTTTTTAATCTGTATGTTAATTTGTTGTTTTTGTACAT80.28591851322373124No Hit
TATAAAAACTATATAAAAAACTTGCTGCCATTTCAGTGATAAAGATAACG80.28591851322373124No Hit
GATTAAAGTGTCTACATGTTAGATAAATACATTGGTTGGCCTATTTCCAA80.28591851322373124No Hit
GGCACATTTTCAAGCTAACTTTATTAGCGACATAAAAGGTACATAAAAAG80.28591851322373124No Hit
CATTTTAGATTTGTGTATCAACCCCTGTGTATTCAACCCAATATCCACAA80.28591851322373124No Hit
TTCAGTTGGAAGAGTATATAGGCGCATCTTTTTCTATTTCGGATGGGGTG70.25017869907076484No Hit
GAAGGAACAGCGCAAGGAAGAAAGAAGAAATAAATGGTTTAAGGAAAAGG70.25017869907076484No Hit
TGAACTTTGAAGACAGCTTAAGGCATTAATTGCACATTATAAAATATATT70.25017869907076484No Hit
TTTGGACAAGGTTCTATATATAGATGGATGTTTTAACACCACTGCGCATC70.25017869907076484No Hit
AAAAAAATACTTAGGGATGTCACTGCTCTGTTATTGTGCAATTGTTTGAA70.25017869907076484No Hit
GTGCATTCACTTGCAGTTATGTGGATGAAACTTGCCATAAGTGTGCATTC70.25017869907076484No Hit
TCTACCCACCCACAAATTAAAATCATAATTATTACATCTACCTTCATTCT70.25017869907076484No Hit
GTTAAACATTTGTCGCCCAATTTTGTATATGCCACGCCAGATTTTCTTAT70.25017869907076484No Hit
CTTGTCAATCGATAAATCTGATAATAAGAATAGCTCCCCCTGGCGGTGAC70.25017869907076484No Hit
ATGCATTTGATATGCGGAAAGCCCTATCCCATGATGTTGAAAAGAAGTCG70.25017869907076484No Hit
CACAACACAAAAATTGATAAAAAACGTATATAAAAAACACAAAAAATATT60.21443888491779842No Hit
GTTAGTTTAAAAAAGCACCAGCAAAATGTCAAAGGTAACCATGCGGTCGC60.21443888491779842No Hit
CTTCAAGCAAGATATAATTATGAAAGTCAACCTGTTGGAACTACTTAAAG60.21443888491779842No Hit
ATACAAATGTATCTAGTCTTCCAAAGGAAGAAATATCCGGACACTAGATT60.21443888491779842No Hit
TAATGAGGACATCAGTGTTATCCGAGGAGACGCAGCAACCCGTGGAAAAA60.21443888491779842No Hit
GATTTATTCTGACTAATTGGTTTACAGTACCAAAGTTTTTATAGCAATAT60.21443888491779842No Hit
TAGACTTCCACAACAGCAGAAAAGATGTGAGCTGGAAGGCCACTACAGAG60.21443888491779842No Hit
CAGTCTTTCTGGTAGAGATGAACTTATCGATTTTGAAGACATTTTCAATG60.21443888491779842No Hit
CTATTGGCGACCACAGCGGACATGTTGGTCTTGGTGTGAAGTGCTCCAAG60.21443888491779842No Hit
AGACGATGCCCCTCGGGCCGTGTTCCCGTCCATCGTCGGACGACCCAGGC50.17869907076483202No Hit
TTTTGTTCCATGTTGTGTATGTTGATCTTCAAAAGTGCTATAGCTGTTAG50.17869907076483202No Hit
CGCGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTT50.17869907076483202No Hit
AACTGTCCATTTGAACAGGGATCAGCTAAACCCGCTTTCTACTATGCTCC50.17869907076483202No Hit
GCCATATGATGACAGATCTCAAGAAGTGGGCGAAAGTAGAAATGAAGGTC50.17869907076483202No Hit
GCTCTGTTTTCCTAGATATTTTTAGCAGCAAGTTGGTACCTTAAAGTTCC50.17869907076483202No Hit
ATGCCTGTGTTGGCGGGGGCTATTACAATATTATTAACAGATCGTAATTT40.14295925661186562No Hit
TGCTGCAGTAACACTGATGATGACAATTCTGACAAACATCATAATGCGTT40.14295925661186562No Hit
GTTTTAAAGGATTTTCTACGTTGCTTATCTATTTCTATATTTCAATGTGT40.14295925661186562No Hit
CCAGGACCTCCACCGGCATCAGGACCACCACCCATGATGCCGCCGCCCAT40.14295925661186562No Hit
GAAGTGCTGCCGTTATGTAAACAAAACTTTATATATTGCACGTGTATCAC40.14295925661186562No Hit
CCCAAATAGGGAAACATATTGTGTTGCGGAAGGAGGACTTCAAATCCTAA40.14295925661186562No Hit
CACACACTCAATTACGGCTGTCCATTGGTGTGTCACGGATTCCAGAACCT40.14295925661186562No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.14295925661186562No Hit
CATAACAAAACGTGTTGTGGATGTCCTGGGGCTTGCAAATCTCTGCAAAA40.14295925661186562No Hit
GATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTACG40.14295925661186562TruSeq Adapter, Index 14 (97% over 38bp)
CTTTTTCCAAATTCGTTTAACTTGCAATGTAAATCTTTTGCTTATTGCATTTTACATATGGGGGATAAATTTACG40.14295925661186562No Hit
GTTTATTTCTGTCTGTGTTACATTCTTGTCAAAATGTACATAGCGTTAGG40.14295925661186562No Hit
GCCAGTAGTTTTTTAGCGAGATCTTTAAAATGAACAAAGTTTCTGGTGGC40.14295925661186562No Hit
ATAAGTCATGCAGATAAAGTCCCTTTTCATAATTATTTTACTATTAAAGA30.10721944245889921No Hit
GTACCAGAGAGAATCATGTTGGCAGGTGGAGGTCAACACATAGGGGCCAG30.10721944245889921No Hit
CCAACACGCATGATTCTGAGACAACAGAGTCCGTATGTGAGAAATTTTAT30.10721944245889921No Hit
GTATTCTGGTAGTGTAAAAAAATAAAGTATATTTTATTTGATACATTTTC30.10721944245889921No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10721944245889921No Hit
CCCCTTACGCCACCTTTTATACCTGTACGTTGAAAATAGAAACGTTGCTT30.10721944245889921No Hit
AAGACACAGACAGCAGAAAAAGCCCGCCTTTTCCCACCACCAATTCCACC30.10721944245889921No Hit
GGAAAAGAAACTGATTTTGATGTTATGAACGCTTCCGTATTCATGCAAAA30.10721944245889921No Hit
CCTTGATCTACTAATGTTACAGAAGTTCATATAGATTTGTTATATGTGAT30.10721944245889921No Hit
ACTTGAACCACCTCGTCTCCGCTACCATGTCTGGAGTCACCACCTGTCTC30.10721944245889921No Hit
GACAAGCTCTGCTCCATGGTAAAGAAATGGCAGTCTTTGATCGAGGCTCA30.10721944245889921No Hit
GTTGTACAAGGATTTGGCAGGAGGAAGGGACCCAATGCCAACTCATAAAT30.10721944245889921No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10721944245889921No Hit
GGGAAACCATCCCCTGAATCTCATTTACAACTTCGTTATAATATAATTCA30.10721944245889921No Hit
GTCACTGCGTCGTCGATGGCGACGAGTGGAGCTTGCCGCTCTCGCGCCAG30.10721944245889921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)