FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935
Sequences flagged as poor quality0
Sequence length42-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC914.702842377260982No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG180.9302325581395349Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT160.8268733850129198Illumina Single End PCR Primer 1 (100% over 50bp)
TGAAAACTTTTCTGGACAAAATAAAAAGATTCGATTTGATATGGAAGAAT130.6718346253229974No Hit
TTTGAACCGGCTAATCAGATGGTCAAATGTGACCCCCGCATGGGAAAATA110.5684754521963824No Hit
AAGACTAATGTTTGTTTGATCTGGGTTTTACGAAAAATCGGCTTTATTTT110.5684754521963824No Hit
GTTTGTTCAAACTGCAGTGATTCGTCAAAAAACATGGCGGCCAGGGGGTG90.46511627906976744No Hit
AAGAAATATGACTTGTGGATTATTCCAGTTTCTTTGATATTTGAACAGTA90.46511627906976744No Hit
ACCGGATAACGATTTCGTCGTCGTGATTTAAAATGTTTTCATCACCAGAA80.4134366925064599No Hit
TGTTGATGTGTTTTTTTTAATCTGTATGTTAATTTGTTGTTTTTGTACAT80.4134366925064599No Hit
TATAAAAACTATATAAAAAACTTGCTGCCATTTCAGTGATAAAGATAACG80.4134366925064599No Hit
GATTAAAGTGTCTACATGTTAGATAAATACATTGGTTGGCCTATTTCCAA80.4134366925064599No Hit
GGCACATTTTCAAGCTAACTTTATTAGCGACATAAAAGGTACATAAAAAG80.4134366925064599No Hit
CATTTTAGATTTGTGTATCAACCCCTGTGTATTCAACCCAATATCCACAA80.4134366925064599No Hit
TTCAGTTGGAAGAGTATATAGGCGCATCTTTTTCTATTTCGGATGGGGTG70.3617571059431524No Hit
TCTACCCACCCACAAATTAAAATCATAATTATTACATCTACCTTCATTCT70.3617571059431524No Hit
GAAGGAACAGCGCAAGGAAGAAAGAAGAAATAAATGGTTTAAGGAAAAGG70.3617571059431524No Hit
TGAACTTTGAAGACAGCTTAAGGCATTAATTGCACATTATAAAATATATT70.3617571059431524No Hit
GTTAAACATTTGTCGCCCAATTTTGTATATGCCACGCCAGATTTTCTTAT70.3617571059431524No Hit
TTTGGACAAGGTTCTATATATAGATGGATGTTTTAACACCACTGCGCATC70.3617571059431524No Hit
CTTGTCAATCGATAAATCTGATAATAAGAATAGCTCCCCCTGGCGGTGAC70.3617571059431524No Hit
AAAAAAATACTTAGGGATGTCACTGCTCTGTTATTGTGCAATTGTTTGAA70.3617571059431524No Hit
GTGCATTCACTTGCAGTTATGTGGATGAAACTTGCCATAAGTGTGCATTC70.3617571059431524No Hit
ATGCATTTGATATGCGGAAAGCCCTATCCCATGATGTTGAAAAGAAGTCG70.3617571059431524No Hit
CACAACACAAAAATTGATAAAAAACGTATATAAAAAACACAAAAAATATT60.31007751937984496No Hit
TAATGAGGACATCAGTGTTATCCGAGGAGACGCAGCAACCCGTGGAAAAA60.31007751937984496No Hit
GATTTATTCTGACTAATTGGTTTACAGTACCAAAGTTTTTATAGCAATAT60.31007751937984496No Hit
TAGACTTCCACAACAGCAGAAAAGATGTGAGCTGGAAGGCCACTACAGAG60.31007751937984496No Hit
GTTAGTTTAAAAAAGCACCAGCAAAATGTCAAAGGTAACCATGCGGTCGC60.31007751937984496No Hit
CTTCAAGCAAGATATAATTATGAAAGTCAACCTGTTGGAACTACTTAAAG60.31007751937984496No Hit
ATACAAATGTATCTAGTCTTCCAAAGGAAGAAATATCCGGACACTAGATT60.31007751937984496No Hit
CAGTCTTTCTGGTAGAGATGAACTTATCGATTTTGAAGACATTTTCAATG60.31007751937984496No Hit
CTATTGGCGACCACAGCGGACATGTTGGTCTTGGTGTGAAGTGCTCCAAG60.31007751937984496No Hit
AGACGATGCCCCTCGGGCCGTGTTCCCGTCCATCGTCGGACGACCCAGGC50.2583979328165375No Hit
CGCGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTT50.2583979328165375No Hit
AACTGTCCATTTGAACAGGGATCAGCTAAACCCGCTTTCTACTATGCTCC50.2583979328165375No Hit
GCCATATGATGACAGATCTCAAGAAGTGGGCGAAAGTAGAAATGAAGGTC50.2583979328165375No Hit
GCTCTGTTTTCCTAGATATTTTTAGCAGCAAGTTGGTACCTTAAAGTTCC50.2583979328165375No Hit
TTTTGTTCCATGTTGTGTATGTTGATCTTCAAAAGTGCTATAGCTGTTAG50.2583979328165375No Hit
ATGCCTGTGTTGGCGGGGGCTATTACAATATTATTAACAGATCGTAATTT40.20671834625322996No Hit
TGCTGCAGTAACACTGATGATGACAATTCTGACAAACATCATAATGCGTT40.20671834625322996No Hit
GTTTTAAAGGATTTTCTACGTTGCTTATCTATTTCTATATTTCAATGTGT40.20671834625322996No Hit
GATACGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTACG40.20671834625322996TruSeq Adapter, Index 14 (97% over 38bp)
CCAGGACCTCCACCGGCATCAGGACCACCACCCATGATGCCGCCGCCCAT40.20671834625322996No Hit
CTTTTTCCAAATTCGTTTAACTTGCAATGTAAATCTTTTGCTTATTGCATTTTACATATGGGGGATAAATTTACG40.20671834625322996No Hit
GAAGTGCTGCCGTTATGTAAACAAAACTTTATATATTGCACGTGTATCAC40.20671834625322996No Hit
CCCAAATAGGGAAACATATTGTGTTGCGGAAGGAGGACTTCAAATCCTAA40.20671834625322996No Hit
GTTTATTTCTGTCTGTGTTACATTCTTGTCAAAATGTACATAGCGTTAGG40.20671834625322996No Hit
CACACACTCAATTACGGCTGTCCATTGGTGTGTCACGGATTCCAGAACCT40.20671834625322996No Hit
CATAACAAAACGTGTTGTGGATGTCCTGGGGCTTGCAAATCTCTGCAAAA40.20671834625322996No Hit
GCCAGTAGTTTTTTAGCGAGATCTTTAAAATGAACAAAGTTTCTGGTGGC40.20671834625322996No Hit
ATAAGTCATGCAGATAAAGTCCCTTTTCATAATTATTTTACTATTAAAGA30.15503875968992248No Hit
CCCCTTACGCCACCTTTTATACCTGTACGTTGAAAATAGAAACGTTGCTT30.15503875968992248No Hit
AAGACACAGACAGCAGAAAAAGCCCGCCTTTTCCCACCACCAATTCCACC30.15503875968992248No Hit
GGAAAAGAAACTGATTTTGATGTTATGAACGCTTCCGTATTCATGCAAAA30.15503875968992248No Hit
CCTTGATCTACTAATGTTACAGAAGTTCATATAGATTTGTTATATGTGAT30.15503875968992248No Hit
GTACCAGAGAGAATCATGTTGGCAGGTGGAGGTCAACACATAGGGGCCAG30.15503875968992248No Hit
ACTTGAACCACCTCGTCTCCGCTACCATGTCTGGAGTCACCACCTGTCTC30.15503875968992248No Hit
GACAAGCTCTGCTCCATGGTAAAGAAATGGCAGTCTTTGATCGAGGCTCA30.15503875968992248No Hit
CCAACACGCATGATTCTGAGACAACAGAGTCCGTATGTGAGAAATTTTAT30.15503875968992248No Hit
GTATTCTGGTAGTGTAAAAAAATAAAGTATATTTTATTTGATACATTTTC30.15503875968992248No Hit
GTTGTACAAGGATTTGGCAGGAGGAAGGGACCCAATGCCAACTCATAAAT30.15503875968992248No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.15503875968992248No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.15503875968992248No Hit
GGGAAACCATCCCCTGAATCTCATTTACAACTTCGTTATAATATAATTCA30.15503875968992248No Hit
GTCACTGCGTCGTCGATGGCGACGAGTGGAGCTTGCCGCTCTCGCGCCAG30.15503875968992248No Hit
TGGTTACAGTAGTTCTTGGTGGGTATTTTTCAGTTCTTCAGTTGGAAGAG20.10335917312661498No Hit
CGTTGACCCATTGGAGCTTGTTTTATTCATGCCTGCCCTGTGCAGAAAGA20.10335917312661498No Hit
TAAGGGTGTAGGTAGACGTTATGCCAATGTTGTCTGCAAGAAGGCAGATG20.10335917312661498No Hit
GGCTGCCCTTGAGAAGGATTACGAAGAGGTCGGTGTCGACTCCGTCGAGG20.10335917312661498No Hit
TCCAAATGGGTGTCATGTTCATTTTATCATTTTTTGTCATGTTCACTATT20.10335917312661498No Hit
CACCACTGGAAGCATCGAATTTCTTACAGCTTGGAAGGAGTACATTCGCG20.10335917312661498No Hit
CTCAACTAAATTGACACAAAAAAAAATCATTAAAAGTTTTAAAAGTTACA20.10335917312661498No Hit
CCCCCCCCCCCCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.10335917312661498No Hit
GTTGCTTTAGCAATTGCGGAGTGTGTATTATAACCTATTTTGTGCCTTGC20.10335917312661498No Hit
CGGTGTGGCGCCCTCTCGTGTCCACCGTACGTCGAAGGCAGAGAATTGGC20.10335917312661498No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCC20.10335917312661498No Hit
CACACACACCACACACACACACACCACACACACCACACACACCACACACA20.10335917312661498No Hit
CCAGAATGTTATTTCAACATTGGAAGCTAAAAGAAAGGTCAAGTCAAAGA20.10335917312661498No Hit
CCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCC20.10335917312661498No Hit
CTGGACCTGTTTCTGTTGTATTATACCAATCTGTACCTTCACGTCCAGAC20.10335917312661498No Hit
TAATGAGGACATCAGTGTTATCAGAGGAGACGCAGCAACCCGTGGAAAAA20.10335917312661498No Hit
GGAAATGGACGTTCATGGTGATATTTCTTTAGCAATACCCTCTGACAAAA20.10335917312661498No Hit
CAAGGAACATCACTGGTGCATTTTCTCAATTATTTAATGACCACAAAAAC20.10335917312661498No Hit
GGGAAATTAAAAAAAAAATCAGGGAATTTTGTTAAATGTCCAGAAATTTC20.10335917312661498No Hit
GGAGATACATACCAGACCCTGCCCCGTAGAATAATCCGCGACTTCAACAA20.10335917312661498No Hit
ATAAAGTTCAATGTTTGGCTGAAGGTGCTTATAAAGAGGCTATGGATGCC20.10335917312661498No Hit
CCTGTTGACAGAAATTTTGGAATACCACGTGGTTCCCCACACTGAATACT20.10335917312661498No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCC20.10335917312661498No Hit
GATGCCTTCTACCTTAATAACGAGCCTCTCCCTGGTGGTTCGGACAACTT20.10335917312661498No Hit
CCAAAGGTACCGGAGTCCCTTCTGAAGAGAAGGAAACACAATGAAGAGGC20.10335917312661498No Hit
CAGCAGAGACCGTATCTAGCACCTTGCTGGCAGAGATATACGTATCTTTG20.10335917312661498No Hit
TTTCTATGGCAAATAATTAATACATGCTTTTTTTACAGAAATATTTGTAA20.10335917312661498No Hit
TGAACAGTAGGGTTCAGCTCAAAGTTGATACTTATTATTGTTTGATTATT20.10335917312661498No Hit
AAATTCCCTTTAAGCCGAGGGTTTATATAGAGAAGAAAAAGCAACTGACT20.10335917312661498No Hit
CTTGATCTGGGTTTTTAATTTGTTTGTCAGTTATTCCAAATCTGCTTAAA20.10335917312661498No Hit
TGGAGCTGTGCCCGATTAGCTCAGTCTGTAGCACATCTGACTATCAATCC20.10335917312661498No Hit
TGACAACAAAATGGCTGTCTGCGTTGGAACAGTTACTGATGACTTGAGGC20.10335917312661498No Hit
GGATGTCATACCTCACTTGTCGTGCGAGGGTTACGTTTAGGGATGGCTTT20.10335917312661498No Hit
CTTCACATTATCAATATGTTCACGTTTTGGTACGCTGATATTACCATGCC20.10335917312661498No Hit
TTTTTTTCGAGAATGATTTTATTTATATTTTTTTAATTTATATAATGCTG20.10335917312661498No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCC20.10335917312661498No Hit
GTTTAATCAAGCTGGGCAAGTTATACTTTACAACTCAACACAAGGGGCTG20.10335917312661498No Hit
TCGTCGGCATACGAGATACGGAACTGTGACTGGAGTTCAGACGTGTGCTCTTCC20.10335917312661498TruSeq Adapter, Index 14 (100% over 37bp)
CGGGGAGATGTACGGGGGAGGCTTACGTCCAGTTTGCGTCCCAAGACATT20.10335917312661498No Hit
TTTTAAACGTGCAATATAATAATAGTTCATACTGTATAGTAACACGGTTG20.10335917312661498No Hit
GTGACATTCAGTTAAACATGAGTGGCAGTCATTTTATAGACGTATTAAAT20.10335917312661498No Hit
GTTACATAAAGGCATCATCTCTGTTAGCGCCATTCATTCGGAGTACCTTT20.10335917312661498No Hit
AATCTATTTAAGAAAAATCTACGCGCATAGCCCAAGTTTGGAAATCGCTT20.10335917312661498No Hit
GGCGGATGGGGAAGTGGGTACGGTCGATAAACTCAGGGTTACATGGAAAA20.10335917312661498No Hit
AACCCAGATGGTTCTGAGGAAGGATAGGCCCACTGTTGATCTCTGCAGAA20.10335917312661498No Hit
GACATGGAAAAAGGAAAGGTACTGCCAATGCCCGTATGCCACAGAAGGTA20.10335917312661498No Hit
TGGCCACCAGGCTAATCATCGTCACGGTGACTGTTTCACCACACAAGAGT20.10335917312661498No Hit
GGACAATCAATCATTCCATGTCGCTAGCCATTCACGAGATATCACACAAC20.10335917312661498No Hit
CGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTC20.10335917312661498No Hit
GGTTGAAGTTATATTATATGATGCATCAAGATTTCTGATTGGTTGAAGTT20.10335917312661498No Hit
GAATATATGGTAACTGTCAGGAACGATAACTCCGGTAGTGACTGATTGTG20.10335917312661498No Hit
AAAAACAAACAAAAAACATTCTTTTGACAGGACTTGCAAAAGTAAGTTAA20.10335917312661498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)