FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1374.896354538956397No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC612.1801286633309505No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG270.9649749821300929TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT270.9649749821300929TruSeq Adapter, Index 14 (97% over 44bp)
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC220.7862759113652609No Hit
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC190.6790564689063616No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC140.5003573981415297No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC120.42887776983559683No Hit
TTTCGCCATCCAATGAAATATTCTGCTAATTTTGCCATCCAATGGAATAT120.42887776983559683No Hit
CAATACAGATAATAATATTTACAGGTAACAATACAGTTAACACAAAATGT100.35739814152966404No Hit
CTCGTTTGAAACACGCCTACTTTTGTATCTATGTAAATCAGCAGCAAATA90.32165832737669764No Hit
TGGTGAAGAAAGAATGAATGAATAGGGTTTTACGGCATACCAACACATAG90.32165832737669764No Hit
CCGGCACTGACCTATTTAATTAGTCAATCGGTTTAATTTGTCTTTGTCAA90.32165832737669764No Hit
GATCAGCGCCCCTTGTATAGCAGTAACGCGGAGAGGACTTTTATTTTGAA90.32165832737669764No Hit
CCAAGACTCAGTCCTCTGCATTCAACTGTTCTGTATATAACTGAACTGAA80.28591851322373124No Hit
TTTTTAAGTTCATACTAATTAAACTTGCACCATCCCCCCGATGGCCATGT80.28591851322373124No Hit
TGCAGACTGCCCGAGGCACCTTTGCGAGATCTCCTCCAGGAACGACAGTT80.28591851322373124No Hit
TGGAGGACCAATAACTGGGCCAATATGACTATTGGAGGACCAATAACTGG80.28591851322373124No Hit
CGCTTCTTTGCCTCACGTTCTTCTATCTCAATCTCGCGTTGACGTTGTTT80.28591851322373124No Hit
TTTATGCTTAAACCTTTCTTTGTAAATATACAACTCCATTTTATGCAATT70.25017869907076484No Hit
CTTACTTGTGAATCGATAAGGAATGTAACATATTGTGTGCAAACAATGGT70.25017869907076484No Hit
GAAAATAAGAAAAATCGGTAAACTCTGTGAAATAAAATTGCTTTTGTAGT70.25017869907076484No Hit
GAAAACTTGTGAAAAGTAACACACGAGTTGTTTCCCTTGCTTTAATGAGA70.25017869907076484No Hit
CATTCATGCTGCTATTTAACGTTTATACAGCAGTTGATGTTTTTGTACAC70.25017869907076484No Hit
CAAATACACTTCCAACAGAAGCACCAACACACCTATAACAAAGACAAGAA70.25017869907076484No Hit
CTTCAACCGATAAATTTAAGTCCTAATTGTGATTTAGTTTCCTTTATTAC70.25017869907076484No Hit
GAATGCACACTTATGGCAAGTTGCATCCACATAACTGCATGTGAATGCAC70.25017869907076484No Hit
GGTAATCCTAAGGGTTACCTGTACGTATGCGCATATGCAGCTGGGAACCA70.25017869907076484No Hit
GTACTGCTTTGAATTTTACCTTTACATATATAATAACCTTGATACCATTG60.21443888491779842No Hit
CCACGTCTCACTGGAATGACAGACAGTTTAGCCAGGATGATGGCACCACG60.21443888491779842No Hit
CTTTTCTATAATTTTTTATCCAATTTTACAAAATTAATAAATCCAAATCT60.21443888491779842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.21443888491779842No Hit
CTTTTTACCACATATCCCCATAATAACATGGGAGATAATACCAAACCCAG60.21443888491779842No Hit
GGCGCACTTCCCTTAGTCGTTAGTACAATTACGATATCCTTGCTTGACTT60.21443888491779842No Hit
GCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAGT50.17869907076483202No Hit
CTTAGATTGGTTTCGACCTCAAATTGACATTTAATTTCTAGACAGACACT50.17869907076483202No Hit
CTTTACTACACAACAAATACAGAATTACAAGATCTAGACCTAACAAAGAA50.17869907076483202No Hit
TTTTCGCCCTGAGTGTAAACTTTGCTCTTGTAGATGCAGAATGCAGATGT50.17869907076483202No Hit
AAAAACAGTGGCAGGTAGTGGTGGTAATCTAATTTTTCAATCTCAACCTT50.17869907076483202No Hit
GCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.17869907076483202No Hit
AACACGTACAAGTGGCAGATAGAGCAGAAATGACATACATGAAATGTAGA50.17869907076483202No Hit
CTTTCTACAGAAACACTTCTACGATCAAACGATGCTGAACCCTGTCTTGG50.17869907076483202No Hit
GTACAAGACGCTTCTTGGCGGCAGCAGTTGTAGTTTGCATTGCCGTATTA50.17869907076483202No Hit
GCAGAATGGCAAATCAACGATATCAAAGTCAACAATATGATTTGGAACCT40.14295925661186562No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.14295925661186562No Hit
CTTACTCCCCCTACATATGATAGTCTCCGCTGCCTTAACAACCTTTCCCA40.14295925661186562No Hit
GCACAACGAGTATAGATAGCTCTAAACAAAAAAATGTTCCATTTTCTTAG40.14295925661186562No Hit
GCTCATTCACAAACCAAATATCAAAATAAGGAATGGTTCAATATTAACAT40.14295925661186562No Hit
CATTTACAGATTGCACAATACACCTAATCATCCATTGCAGACGGCATCAA40.14295925661186562No Hit
ATAAAGTATATAATTTTCAATATAGGTCCAATGGGGGATCATATTGTTTC40.14295925661186562No Hit
ATACAATGATGTGTGATAAACAATCGCAAAACGTCTTTTTGTGAACGATT40.14295925661186562No Hit
CTCATTACACTCCTCGGTGCTCTATGTTATATATAACAGGTAAAATAAAA40.14295925661186562No Hit
GCTTGAGCTATGAAATTTAAATGCGAAAAGTACTACGAATGCTGAGTTAT40.14295925661186562No Hit
GGTTCGAGTTTTTATTTTCTACTGCTATTATGTATATTAATAGAAAAAAA30.10721944245889921No Hit
CGTAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCC30.10721944245889921TruSeq Adapter, Index 14 (97% over 38bp)
CTCTCATCGGGTAGGTCATACGTTTCATTTTCCTCTTCTGCAGTTTCTAA30.10721944245889921No Hit
TGGTTACACAAGACATACTATTTATCTGAACAATATCAGTTCACAAGATG30.10721944245889921No Hit
CAACCAGTAACTTTATACAGACAACGGGCAATCGTGAAACCTCGGGTATT30.10721944245889921No Hit
CGTAAATTTATCCCCCATATGTAAAATGCAATAAGCAAAAGATTTACATTGCAAGTTAAACGAATTTGGAAAAAG30.10721944245889921No Hit
ATTGTCGCTTCTTATGACATGCATAAGCCACAGTGATCCTATTCTTGCCA30.10721944245889921No Hit
AGACACATAATACTTGAATTACACGTAACATACCAGTTGTATGTCCTTCC30.10721944245889921No Hit
CTGTTGTCTGGGATAGTAACCTTGAGACATTGCAATATTGCGTGGCACTG30.10721944245889921No Hit
GGTACTTCAAAAATTCAAAACGCAATTTGCTAGTATTGGTCTCGTTTCAT30.10721944245889921No Hit
AAACTGTTCTTCAAGAGTTAGTGTTCTTGGTGGAGTCAATAAACCAACAT30.10721944245889921No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCC30.10721944245889921No Hit
CAACATTTCAGTGTCCGACAAGACTAGTAAAGTTTTGTTTATTTAAACTA30.10721944245889921No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACGA30.10721944245889921TruSeq Adapter, Index 14 (97% over 40bp)
ATGAAACATAAAAAGATTATGTGATGGATAAAAGAAACATATATAAAAAG30.10721944245889921No Hit
GTTATCTGTGCCCATATGCCTGTCCTACAGGGAATTTTCATACATCAGTC30.10721944245889921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)