FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-OA-exposure-RNA-EPI-115-1_S4_L001_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935
Sequences flagged as poor quality0
Sequence length42-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC864.444444444444445No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC341.757105943152455No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT150.7751937984496124TruSeq Adapter, Index 14 (97% over 44bp)
TCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC140.7235142118863048No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG140.7235142118863048TruSeq Adapter, Index 14 (97% over 43bp)
TTTCGCCATCCAATGAAATATTCTGCTAATTTTGCCATCCAATGGAATAT120.6201550387596899No Hit
CAATACAGATAATAATATTTACAGGTAACAATACAGTTAACACAAAATGT100.516795865633075No Hit
CTCGTTTGAAACACGCCTACTTTTGTATCTATGTAAATCAGCAGCAAATA90.46511627906976744No Hit
TGGTGAAGAAAGAATGAATGAATAGGGTTTTACGGCATACCAACACATAG90.46511627906976744No Hit
CCGGCACTGACCTATTTAATTAGTCAATCGGTTTAATTTGTCTTTGTCAA90.46511627906976744No Hit
GATCAGCGCCCCTTGTATAGCAGTAACGCGGAGAGGACTTTTATTTTGAA90.46511627906976744No Hit
TTTTTAAGTTCATACTAATTAAACTTGCACCATCCCCCCGATGGCCATGT80.4134366925064599No Hit
TGCAGACTGCCCGAGGCACCTTTGCGAGATCTCCTCCAGGAACGACAGTT80.4134366925064599No Hit
TGGAGGACCAATAACTGGGCCAATATGACTATTGGAGGACCAATAACTGG80.4134366925064599No Hit
CCAAGACTCAGTCCTCTGCATTCAACTGTTCTGTATATAACTGAACTGAA80.4134366925064599No Hit
CGCTTCTTTGCCTCACGTTCTTCTATCTCAATCTCGCGTTGACGTTGTTT80.4134366925064599No Hit
GAAAACTTGTGAAAAGTAACACACGAGTTGTTTCCCTTGCTTTAATGAGA70.3617571059431524No Hit
CATTCATGCTGCTATTTAACGTTTATACAGCAGTTGATGTTTTTGTACAC70.3617571059431524No Hit
CAAATACACTTCCAACAGAAGCACCAACACACCTATAACAAAGACAAGAA70.3617571059431524No Hit
CTTCAACCGATAAATTTAAGTCCTAATTGTGATTTAGTTTCCTTTATTAC70.3617571059431524No Hit
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC70.3617571059431524No Hit
TTTATGCTTAAACCTTTCTTTGTAAATATACAACTCCATTTTATGCAATT70.3617571059431524No Hit
CTTACTTGTGAATCGATAAGGAATGTAACATATTGTGTGCAAACAATGGT70.3617571059431524No Hit
GAATGCACACTTATGGCAAGTTGCATCCACATAACTGCATGTGAATGCAC70.3617571059431524No Hit
GGTAATCCTAAGGGTTACCTGTACGTATGCGCATATGCAGCTGGGAACCA70.3617571059431524No Hit
GAAAATAAGAAAAATCGGTAAACTCTGTGAAATAAAATTGCTTTTGTAGT70.3617571059431524No Hit
CCACGTCTCACTGGAATGACAGACAGTTTAGCCAGGATGATGGCACCACG60.31007751937984496No Hit
GTACTGCTTTGAATTTTACCTTTACATATATAATAACCTTGATACCATTG60.31007751937984496No Hit
CTTTTCTATAATTTTTTATCCAATTTTACAAAATTAATAAATCCAAATCT60.31007751937984496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.31007751937984496No Hit
CTTTTTACCACATATCCCCATAATAACATGGGAGATAATACCAAACCCAG60.31007751937984496No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.31007751937984496No Hit
GGCGCACTTCCCTTAGTCGTTAGTACAATTACGATATCCTTGCTTGACTT60.31007751937984496No Hit
GCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGAGT50.2583979328165375No Hit
AACACGTACAAGTGGCAGATAGAGCAGAAATGACATACATGAAATGTAGA50.2583979328165375No Hit
CTTAGATTGGTTTCGACCTCAAATTGACATTTAATTTCTAGACAGACACT50.2583979328165375No Hit
CTTTACTACACAACAAATACAGAATTACAAGATCTAGACCTAACAAAGAA50.2583979328165375No Hit
CTTTCTACAGAAACACTTCTACGATCAAACGATGCTGAACCCTGTCTTGG50.2583979328165375No Hit
GTACAAGACGCTTCTTGGCGGCAGCAGTTGTAGTTTGCATTGCCGTATTA50.2583979328165375No Hit
TTTTCGCCCTGAGTGTAAACTTTGCTCTTGTAGATGCAGAATGCAGATGT50.2583979328165375No Hit
AAAAACAGTGGCAGGTAGTGGTGGTAATCTAATTTTTCAATCTCAACCTT50.2583979328165375No Hit
ACCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.2583979328165375No Hit
ATAAAGTATATAATTTTCAATATAGGTCCAATGGGGGATCATATTGTTTC40.20671834625322996No Hit
ATACAATGATGTGTGATAAACAATCGCAAAACGTCTTTTTGTGAACGATT40.20671834625322996No Hit
CTCATTACACTCCTCGGTGCTCTATGTTATATATAACAGGTAAAATAAAA40.20671834625322996No Hit
GCAGAATGGCAAATCAACGATATCAAAGTCAACAATATGATTTGGAACCT40.20671834625322996No Hit
GCTTGAGCTATGAAATTTAAATGCGAAAAGTACTACGAATGCTGAGTTAT40.20671834625322996No Hit
CTTACTCCCCCTACATATGATAGTCTCCGCTGCCTTAACAACCTTTCCCA40.20671834625322996No Hit
GCACAACGAGTATAGATAGCTCTAAACAAAAAAATGTTCCATTTTCTTAG40.20671834625322996No Hit
GCTCATTCACAAACCAAATATCAAAATAAGGAATGGTTCAATATTAACAT40.20671834625322996No Hit
CATTTACAGATTGCACAATACACCTAATCATCCATTGCAGACGGCATCAA40.20671834625322996No Hit
GGTACTTCAAAAATTCAAAACGCAATTTGCTAGTATTGGTCTCGTTTCAT30.15503875968992248No Hit
GGTTCGAGTTTTTATTTTCTACTGCTATTATGTATATTAATAGAAAAAAA30.15503875968992248No Hit
AAACTGTTCTTCAAGAGTTAGTGTTCTTGGTGGAGTCAATAAACCAACAT30.15503875968992248No Hit
CTCTCATCGGGTAGGTCATACGTTTCATTTTCCTCTTCTGCAGTTTCTAA30.15503875968992248No Hit
TGGTTACACAAGACATACTATTTATCTGAACAATATCAGTTCACAAGATG30.15503875968992248No Hit
CAACCAGTAACTTTATACAGACAACGGGCAATCGTGAAACCTCGGGTATT30.15503875968992248No Hit
CGTAAATTTATCCCCCATATGTAAAATGCAATAAGCAAAAGATTTACATTGCAAGTTAAACGAATTTGGAAAAAG30.15503875968992248No Hit
CAACATTTCAGTGTCCGACAAGACTAGTAAAGTTTTGTTTATTTAAACTA30.15503875968992248No Hit
ATTGTCGCTTCTTATGACATGCATAAGCCACAGTGATCCTATTCTTGCCA30.15503875968992248No Hit
AGACACATAATACTTGAATTACACGTAACATACCAGTTGTATGTCCTTCC30.15503875968992248No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGACGA30.15503875968992248TruSeq Adapter, Index 14 (97% over 40bp)
ATGAAACATAAAAAGATTATGTGATGGATAAAAGAAACATATATAAAAAG30.15503875968992248No Hit
CTGTTGTCTGGGATAGTAACCTTGAGACATTGCAATATTGCGTGGCACTG30.15503875968992248No Hit
GTTATCTGTGCCCATATGCCTGTCCTACAGGGAATTTTCATACATCAGTC30.15503875968992248No Hit
TTCGGATCTGAACGGCGCCCCAATCAGAAGATCATCCATTCCATCTCCAT20.10335917312661498No Hit
GGATGCGTATTATATATGTCCCATATTGCCAACTTGCTCACTGACAGTAA20.10335917312661498No Hit
TACCCAAGCACTTCCCATCTCCGTAAGTAACTGATAACTTTTCCACTTGA20.10335917312661498No Hit
TGCCGACTGCCCGAGGCACCTTTGCGAGATCTCCTCCAGGAACGACAGTT20.10335917312661498No Hit
TTTTTTTTATCTGTTTATTTCAACTTTATTTCCATTACTATGTACAAGAC20.10335917312661498No Hit
TTTGGATCCGCGCATGGATTTACTTATTGAAAAGAATTTGTAACTTGTAT20.10335917312661498No Hit
GTGAAGATGCTTTCTTTGGATCCAAGTTTCCAATCCCCATCCACAAAGAA20.10335917312661498No Hit
CTGTCACTTTCAATGCACATAACTTCAGTTTTGGGACCTGTAGAAGCCTC20.10335917312661498No Hit
CTCTGTATTGCTGGCTACCCCTAGATGTAAGAGGAGCGAATCCAGGCATG20.10335917312661498No Hit
TTACAGTATAGAAACAATGTAAGTACCGGTAATCGTTATACATATATTTA20.10335917312661498No Hit
TCTGGACCTGTTTCTGTTGTATTATACCAATCTGTACCTTCACGTCCAGA20.10335917312661498No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCC20.10335917312661498No Hit
TGATACTTTGTTTAATTGATTCTTGTCCTCAGGATGAACTGTTGTTAATG20.10335917312661498No Hit
ATTCTGTCGTGGTGTCTGTCCAGTGTTCTTAGTGTTTCCCTGTTGTACAA20.10335917312661498No Hit
ATCTAACTTATTAAACTGTTGGCAGTTAAAAATACTGGAGAGGTTTATCA20.10335917312661498No Hit
GGACTATTCTGCATCTGTCCTGGGGGCATGCCCGGGGGTGGGGGACGGGT20.10335917312661498No Hit
AAAAAGTATAAACACAGTTTGAAATGATTGAAATGAAATGCAAAATGGGT20.10335917312661498No Hit
TCCGGTTCTGGGAATTTAGTCTTGGAGACACGGGCACCACACATTCTCTT20.10335917312661498No Hit
TCTACTTGGTATATCAACGTCTTTATCGTCATCACCTTGGTAACGATGAT20.10335917312661498No Hit
TGACTGCCAGCTGAATTGTACTCCCATTTGTCTTCAGGGGCGCCGAGTTT20.10335917312661498No Hit
TCAGGTTCTGGGAATTTAGTCTTGGAGACACGGGCACCACACATTCTCTT20.10335917312661498No Hit
AGTACCTCATTTGCTGAATTCTCTGAACATTTGAAAGAAAAACACCCACA20.10335917312661498No Hit
GCCACCTTCAGGAACATGTTGAGCTGCTGGTTCGTAAACTCAGGATTGTC20.10335917312661498No Hit
CGAGACGCCGCTCACCGCCGCCGCGCGGAGCATGTTGATCGAGCAGTAGG20.10335917312661498No Hit
CTTTTCCCTGTTAGCCTTGGGGTTGAGCGGGGCTTCGGTAAGTAGAACGG20.10335917312661498No Hit
TTGAAGCCCAGTACAATCCACGCTCGTTCGGGTCGGGTTCGTACGGATGA20.10335917312661498No Hit
TTAAAATGGACACGTTACTAAACAAGAAGTCCATCCATTATCTTTCAAGT20.10335917312661498No Hit
CTCATCTAGAAACTATATTATAAAAGAAATATGCATCAGGATAATCAGAA20.10335917312661498No Hit
TGCTGATTGTGTTACACAATAAATGTTTGTTTACATGCTCACACGTGGTG20.10335917312661498No Hit
GAACAGTTTCAGCTTCATGTTATTAATATCCAGAAGCAGGTATCTTTCAT20.10335917312661498No Hit
CCGCAAGATCGTGTAAGCAAATAAGAAATACCACTCTGGTTGAATGTGGA20.10335917312661498No Hit
TGAACAGCAATGTTATTATAAAATTATTATGATTATAATCATAATTATTT20.10335917312661498No Hit
TACTGGTAGACCCCATTAATGGCACAACTTACAAGACTTTCTCATATAAA20.10335917312661498No Hit
GGAATATTCTGCATCTGTCCTGGGGGCATGCCCGGGGGTGGGGGACGGGT20.10335917312661498No Hit
CGTAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTC20.10335917312661498TruSeq Adapter, Index 14 (97% over 38bp)
GTAGCCATGGTTGTGGGGGAAAGGTGACAGCTTAATTCTGTAATGCTTAC20.10335917312661498No Hit
ACTTAATGTTGATAGATTAGCACACAAAGGATTAATTATTTGGCAAATTT20.10335917312661498No Hit
GGATATGTTCCATCAAGACTCTCTTGTTCTTGAAGCCGTTTCCTTTGGAC20.10335917312661498No Hit
CCGCCAGATCGTGTAAGCAAATAAGAAATACCACTCTGGTTGAATGTGGA20.10335917312661498No Hit
CCCTCAGCCAAAGATCCTAACCTAAAATGAAAAAAGGACCAAAAAAATGA20.10335917312661498No Hit
CCGCAAAGTGATTCATGTAAGTCCGAACTAACCCAACTAGTAAAAATAGA20.10335917312661498No Hit
TTAATTAAAGATTTAAGATGTTCCAACTCAAACTAAAATTGCCATTTTAA20.10335917312661498No Hit
CAAAGATCCTAACCTAAAATGAAAAAAGGACCAAAAAAATGAGACAAAAA20.10335917312661498No Hit
TGATAATCGTGTCACTTGCTGAACATTAACCTACGACCTGCACAATCAGT20.10335917312661498No Hit
TTTTACTGGTATACCATCCTTATCAATGAGAAACTTCGTAAAATTCCACT20.10335917312661498No Hit
TAAAAAAATTAATTTTCAGTATCGTCTGTCTGTTGGACTTCCTGATGTTG20.10335917312661498No Hit
GAAACTTTCATGCACTTAAGCATATACAAACAAGTACTGAAGCTAAACAA20.10335917312661498No Hit
GGACATACCATAATCCATATTCATCACCATAGTAGCCTCATTTAAGCTAC20.10335917312661498No Hit
CGGCCATAGCCGGTAAATGTTCCAGTGAATCCGGCAACACCACCGGTTCC20.10335917312661498No Hit
AGCAGGTCTTCTTGACTTGGTATGGCTTTTTCTTGGTGAATCCGATAGAA20.10335917312661498No Hit
CAAACTAAGAGCCCCATCAATAAACAAAGATAAACAAAAAAATCCAAACT20.10335917312661498No Hit
CAACCTTTCAGTGTCCGACAAGACTAGTAAAGTTTTGTTTATTTAAACTA20.10335917312661498No Hit
CGGGGATGTACACCATTTATACCTCTGATTCTTGTGACAAAGGCCAACTT20.10335917312661498No Hit
ATAAACTAGAACATTCAAAATGTACAAGCATAAACATAAATAGAAGCGAA20.10335917312661498No Hit
TACACTATGGGCACTAAGCTACGCAGGCCTGTACTTAACTTGAGAAAGCA20.10335917312661498No Hit
CTTTTCAATTCATAATTTTGGCCTGTTTAAACAAATCTTTTTACATATAT20.10335917312661498No Hit
CTATATACTGCTGATAGTAACAGTAATGCTATGTTGACAATATACCGACA20.10335917312661498No Hit
ACAACATATTTAATACCAAAAAACATGACAAAAATTTCGTTGAATAATCG20.10335917312661498No Hit
GCCCAAAACACCTCTCATCAGCATTGTGGAATCCTGCCGGCCATACCTGA20.10335917312661498No Hit
AAAAAAATATAGTTGCTTGATCGTTCGCTTTTTAGAGCATGTGCGCATTC20.10335917312661498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)