FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-heart-RNA-8_S58_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-8_S58_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19573
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2021.0320339242834518Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT540.2758902569866653Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.22479946865580136No Hit
AGAGGCTGGAGCGTAGGATCGAGATGTTTGAGCTAGAGAGAGACGAACAA350.1788177591580238No Hit
AGAAAAGAACCCCATGCGGGATCTCCGCATTGTAAAACTGTGTCTTAACA350.1788177591580238No Hit
GTCGCGTCGAGGTGAAATATAACGGTGTCTGGGGAACAGTATGTGACGAC340.17370868032493741No Hit
GAATCATCGCCCAGCCACAGTCTCCATGATGATTGATATACAACATTGCA330.16859960149185102No Hit
GAAAAGTTATATTGTGGTTAAACAAAAATATCATGCTACAATGATTATGT320.16349052265876463No Hit
GCAAAGACAGGCCTCGGCTATGCTGTCGATCATAAAATGGAGAACAATTA320.16349052265876463No Hit
CTACACATATGTTTGTTTGATGGATATATGTATGACGGGTTTGTTTATAA320.16349052265876463No Hit
TGAACATATTATATGCTGAAGCATTTTGCTCGAATGTGTTCGCTCAAACT320.16349052265876463No Hit
GTGAAGCTCCTTCAGAGCCGAGGAATCCTTCCTGGAATCAAGGTTGACAA320.16349052265876463No Hit
GCAGATTACAACTAAGATCACGAAGGGATGCATTGAAATTCTTAATGATG310.15838144382567823No Hit
TGTTGATTCACTAAACTGAACTAAATAAAACAATATCATCATAAAGTTTG310.15838144382567823No Hit
AACTTTTCAATGTACGAAAAATAAAGTAACGACGTGATAACATGTTTGAG310.15838144382567823No Hit
CGAAAATTGGCAACATAAAAGAAATACGAAAGAATGTCTCTTAAATCACT300.15327236499259184No Hit
ACGAGTTGTGGTCGTGAGATGTGAAGGTATCAACATTTCTGGAAACTTCT300.15327236499259184No Hit
GACGAATCAAGATTGTCGTATATCTATCCAATATTTGGAACTATCTGTAT300.15327236499259184No Hit
TGCAGGTTTTCTTGAACCAATCTAAATGCTGTACATGTTGAATAATAGAT300.15327236499259184No Hit
CCCAACTCCAAAAGCTAAACCAACGCGTAAAGTGCGCGACGAGCTACCAG300.15327236499259184No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300.15327236499259184No Hit
CTGCTAGGTGTGGTGGGTTAAGAATAGCTGGGTCTTTAATCAGTTGAATA290.14816328615950544No Hit
GTAATTTACAATTATGACAGATGGGCGTTGTTTGTGACGTCGTCAAATTA290.14816328615950544No Hit
TCAGCTCTCCCATACAGAAGATCTGTCCCAACTTGGTTGAAGCTTACATC290.14816328615950544No Hit
CTTTTTGTGACTGTGGGTCCATATTACTTCCGTTGGACCGTAGTTTTTCT290.14816328615950544No Hit
CTTAACAAGATGATTCTAGTCTCATTTTGCCCGTCATTTTGTTTTAAATG290.14816328615950544No Hit
AAACCCTTATTAAATATATTTGTGTAATGTACATAAAATGTAAAAGTTAC290.14816328615950544No Hit
GCAAATGATGCCAAGTTGAAGGAGATCGTCACATCATTCATTGCTTCATG280.14305420732641905No Hit
CTTTACTTTATCAGAATTTGCGAAAACAACTTAATTCTATAATCACGTTC280.14305420732641905No Hit
GGCAAATTCAAAGTCCCATATGCCAGCTTACACGGGAGAAATTACCAAGA280.14305420732641905No Hit
CCAACGCAAATTTTCATCTACACGTTCATGAGTTCGGAGACACTACCGGT270.13794512849333265No Hit
TGTGAATATACGGCCAGAAATTATAGAGTTAAAGCGAGTTAGCTTATTTG270.13794512849333265No Hit
CTTCTTTCCGCTTCAAATTCATTGTTAAACGCCTCTTTTAAAAGAGGAAT260.13283604966024626No Hit
CGGGAAGAAATGTCAAACTCCCTGAAGATCGTGAATATTGAGGAGATTCC260.13283604966024626No Hit
CGCCATTCTAGTCTATGATATTACGGACGAAGATTCATTCCAGAAGGTGA260.13283604966024626No Hit
AGAAGTCTCCATCAACCTTAATACATTGGCAGCCAAACAGCAAAGTATTG260.13283604966024626No Hit
CCGGCCCATCCATCGTCCACAGGAAATGCTTCTAAGTTATTCGATCTATT260.13283604966024626No Hit
GCAGTATCGTCCTTATCTTATTTCGGCAACTTTGTAATAAACGGCATATC260.13283604966024626No Hit
TGAAAGCCAAAATTCAAGACAAAGAAGGAATTCCCCCAGATCAGCAGAGA260.13283604966024626No Hit
TTGGTTCTTGATAAGAATAAAATAACTGTCAGTGTCTGCAGAGGGGTAGG260.13283604966024626No Hit
TGTTTTTACACTACTGCAACTCTTGTGGCCCAAACTACAGTTGTGAAGTG260.13283604966024626No Hit
GTTAGCGCAGTCTGTAGAGCATCTTATCAATGCAGGGATTGCAAGTTTTA250.12772697082715986No Hit
CTCATCTTCTCTTGAGAAGAGCTACGAACTCCCCGACGGTCAAGTCATCA250.12772697082715986No Hit
GTTTTACCCAATCAAACCAATGAGACGTATACCAATAAAATGTGCCAAGT250.12772697082715986No Hit
TTTATAAATCAGATGTCGGAAGACCAATTCTTCCAGTGGTTGATGAAAAG250.12772697082715986No Hit
CAACACTGAGTATTGGTTCTGTGCAGGAAACAACACTGAGTATTGGTTCT250.12772697082715986No Hit
AATTCCTTGTGTTATAACTAGAAAGACAATAGTACTACGCAAGTTACACA250.12772697082715986No Hit
CAGGCGTCAGAGAAACGTGTTGACCTCGTGAAGCATGTCTGCTCCAACAC250.12772697082715986No Hit
CCAGGTATTTTGGAGACAGTGTTACTTTTAGGGACAAAAACTAGTTACTT250.12772697082715986No Hit
CGTACCCGAGATGAAAGTTGGTGCAATCAATTCTAAAAAAAAAATAATGA250.12772697082715986No Hit
GGTGAATTATGTTTATGTTAACTGACAGAGTGGAAAACAGTTCAGGAAGT250.12772697082715986No Hit
ATAAATTCTTTATGTATATTGTTATTGGATGTGATATTGCTTTTTACAAA250.12772697082715986No Hit
GTTTGTTGCTGGTTTGATTGTACTGCTATTGGATGAGTTGTTACAGAAGG250.12772697082715986No Hit
AATCTGATCAATTACCTTAAAGTGTAGTTTTGAATCACATTTAAAACCTA250.12772697082715986No Hit
TCGCCTTCCAGGCCGATGAGGATAGAAAGAACCAGGAACGTCTCCAGGAC250.12772697082715986No Hit
GTTGGAAGAACTTGTTTCTTAATTAACATAAAATATAAAGTTTAAACTGT250.12772697082715986No Hit
CTTGACCAGGTATAATCTGCACATACTACTATGTATTATTATATACATGT250.12772697082715986No Hit
TGAAATTTCACAAAATCATGGGTTTGTAATGAAGGAGACAAACTTTTAAA250.12772697082715986No Hit
AGGAAATGTTGTTAAAGATTTGACAGGTAGAGTAGTTAAATGTTTACCTT240.12261789199407348No Hit
TGAATGTCCATGTTACAGAATATCATGTCCCCATAATTATGAATGTCCAT240.12261789199407348No Hit
TAGACTCATGTGATAGTTTAGGACAATATAGTGTTACTATCAACTAGTGT240.12261789199407348No Hit
GGCGAGCTTATTTTAGTGCGGCAACTATGATTATTGCTATCCCTACAGGA240.12261789199407348No Hit
GGCAAAAACGCTATTTTCTAATAGTTCTACTGTCAGTAGAAATTATGGTA240.12261789199407348No Hit
TGATTGTTTAAGATAAGTGGCTGCTCAATAGAGGTGGTCGCTTACACAGG240.12261789199407348No Hit
CACGGAACGAGGATTTCATCTTCTGAAAAGTGAGTTTAAACTGAAACAGA240.12261789199407348No Hit
TAGTTACAAACTCATCACACCATCTGTTGTGTCTGAAAGACTGAAGGTCC240.12261789199407348No Hit
GTGAAGTAAAAATATTGTAAATGCCCACTGCAAAATTTGAAAAGTCTGTG240.12261789199407348No Hit
CGTGTTTACAGGAAGGAGCTTTCATTCACGGACGTCAAAATTGTTAACCT240.12261789199407348No Hit
TTCTGCTCCTGATATGGCATTCCCTCGAATAAATAACATGAGGTTTTGGC240.12261789199407348No Hit
GTAAGGCATAGATGGATTTTGTTGATACTTAGTACAAATGATCAACATAA240.12261789199407348No Hit
AACGACATCAGAATGGCGTGGTCAGGTATGGCCGGCAGGATTCCACAATG230.11750881316098707No Hit
TGTAAATACATCTTGGTCCGTAAAAGTTCTACACTATTTTGATATTATGT230.11750881316098707No Hit
TGAGAATAATCTTTGCCCTTCTATAAGTAATTGCCAAGTATTCTAACATT230.11750881316098707No Hit
GTTTTAATAAAAAAAAATATCCAAGAATAATGGAGTGCATGGTGTATTGA230.11750881316098707No Hit
CGGTAGTTCATCTTATTTTTCTTCATCAGACAGGGTCAAACAATCCTTTG230.11750881316098707No Hit
CTGACTTCCCAAAATATTATAAAGGACGTTAAATTGTTTATTTTCCTGAG230.11750881316098707No Hit
GTTCGGGTCCGAATGCAAACACATGTGTCATTGCAAGGACGCCAGAGAAC230.11750881316098707No Hit
TTGAGTGCTGCCATATTGGATTTGCTGTTCTGTCATACATGTGTATTATT230.11750881316098707No Hit
GCCATCCAGGCCGTACTCTCCCTGTACGCTTCTGGTCGTACCACTGGTAT230.11750881316098707No Hit
TGGTTATGGTGTGTATACTTTTGTACACAAACTTTACATATGCCTCTTGT230.11750881316098707No Hit
GTTAACATGTTTTGTTTCAATACTCTTCTTCTAACACAGATTGTTACCAT230.11750881316098707No Hit
GGAATGTTGTTATTAAGGGTGTTGGGGTTCTTTGTGTTGAGTTTATGCTT230.11750881316098707No Hit
CTTCCATGGCATGGACCTCACCCGTGACAAGCTCTGCTCCATGGTAAAGA230.11750881316098707No Hit
GTTTGTCGCGCCTTGGGCATTCCAACACGTAGCGTTACAAACTTTGAATC230.11750881316098707No Hit
GTTCGAGCAGACACCATCCTGACTGGCGAAATGAAGAACTTGACAGAGAA220.11239973432790068No Hit
GACTGGGCCTCCTGTGAATCTAGCTGGGTCTATGAATAATAACAGTTAAT220.11239973432790068No Hit
TGCGTAGCAGTTTAATTAGTATAGACTGTTTCTGCATTCACACAATTTCC220.11239973432790068No Hit
TTCGCAGGTCGTCAGTAGTAACTGAACTACCTGCCGAGTCCATTTGGCGG220.11239973432790068No Hit
TTCAAAGTTCCGTACCCGAAATCATTCATAGAAACTTATATCTCATTTTT220.11239973432790068No Hit
GGAGCACATGTCAAGGTATGTTTGTACAGTTGAAACTGATGATGAAGGAG220.11239973432790068No Hit
CGGAAAGAGGCATAACAAAACCCACACACAGTGTCGGCGATGTGGGCGCC220.11239973432790068No Hit
GGTGGTTAGAAAGATAATATTTAGACTTGCTTTAAGATTTTGGGGGGGAA220.11239973432790068No Hit
AAATTGCTGCCATAGCACAGGAATTAAACACATTAGAATATCACGACATA220.11239973432790068No Hit
GCATATATATTTATAAAACATTATTTATTTTATATTTATCTCATGTCGAT220.11239973432790068No Hit
GTTTTATATCTGTCTTTGCTGATTCTGGCTGCATGTTCGATACACTATAC220.11239973432790068No Hit
GTCTTATATACAGTCAAACCTGTGTAAGTGGTCACCTCTATTAAGCAGCC220.11239973432790068No Hit
GTTTATTGCAGTATTCGTTCTTGTACAATATTTGACCTCCGTATTTAGTT220.11239973432790068No Hit
CAGAACATGGGCAATGTGCTACAGCTGTGTATGATTACCAGGCTGCTGAT220.11239973432790068No Hit
GATGTATACTTTATAGAATAGATCAAGACAGTACGGAGCTTTTGTCAGAA220.11239973432790068No Hit
GTGTAAACCACCAAACACGAACTTTACATTTGGTGGCGTTTACCAGAAGT220.11239973432790068No Hit
AAAAGATTCCATTGGTTGGCGCCTTTAAAAAGATTCCAATGGTTGGCGCC210.10729065549481427No Hit
AATGCATTTTGTCTATGGTATTTATTTCTCTATCTAATTACGATACATAT210.10729065549481427No Hit
AACAGCAGTAGTATTCTGAGCAACTGTAGTAGTTACATCTGTACTGTGTT210.10729065549481427No Hit
ACTCCATCCTGACCACCCATACCACCCTGGAGCACTCCGACTGCGCCTTT210.10729065549481427No Hit
ATACCTTTTTCACATCCTTTAGGATAAATAAATAAATATGTGTGACGACG210.10729065549481427No Hit
GAGAAATATTACACACACATGCTGCTTTGTCACAGATTTTAAATTAAATT210.10729065549481427No Hit
TGAACACATTGCTGTGAAAGCAACAATATTTAACTACGGCAATCCCGGAG210.10729065549481427No Hit
GCAAGATTAGCGGATAATCTTTCTAAACCGAAGATAATTTTGGCTGTGAA210.10729065549481427No Hit
TTAATGAGGAAAAATCATATTTAAATTTTATAGTGAAAACCTCATTGTTT210.10729065549481427No Hit
TTTTAATTGCTATAGGTATGTCTTTTGAAACAGTTTTCGGTTGTTCTTTA210.10729065549481427No Hit
GGTGAGTGATTCGTAATTGCCATGTTAATGGGGCTTCGTTGTTTTTTAGT210.10729065549481427No Hit
GTTGACTGTGTAGAACTAACCATAAAGACATTGGAGTCTTATATTTTATC210.10729065549481427No Hit
CATGGATCTCATTGCCAAACTGCCAACCGTTGCTGCCATCATTTACCGTA210.10729065549481427No Hit
CCAGACGCTACATGGAGCGCCAAGCTGATTAGTGGAAAGAGGGTACCAGT210.10729065549481427No Hit
GCAAGATCGTGAAAACCAGTCTATGTTGATCACCGGGGAGAGTGGTGCAG210.10729065549481427No Hit
ATGCTATTGTTTGGACTGACCAATACTACGATTCTTGTAAAAAGTTGTTG210.10729065549481427No Hit
TGGGGGTATGAATAAATTATCTCTGTGGTCTGCATCATTTTGTCAACAAT200.10218157666172789No Hit
CTAATACAAGTAATGAACACAAGGTTCAAAGTAAACTCCAGAACATTAGT200.10218157666172789No Hit
CGAGAGGATTGACAAAGAACCAGGACCATGGAAAAATTATACAGACCTCT200.10218157666172789No Hit
AAAACGACTGACACACGTCTTCGTAAACCTCATCTCACCATTAAGGCGCC200.10218157666172789No Hit
TTAAAATGGCATTGTAGCAGAAAAAATTCCCGGCAAAGGAAAAAATGTTT200.10218157666172789No Hit
AGATATAAGTCCTTGACCCTTAACTGCTAGGTGTGGTGGGTTAAGAATAG200.10218157666172789No Hit
GTGTGCTCATGCATACATGTGACATTATAAAGGTGTTACTATATGTACAG200.10218157666172789No Hit
GGGAAAACTGCCATAAATAAAATGATTATTAAAATGGATAAAAGCTATCT200.10218157666172789No Hit
TGTTGAATAAACTTTTTAATGAGTTTGTTAAAGAATACCAATCTTTTTCG200.10218157666172789No Hit
GCCATCACGAACACTTTATACCTCCGACACGAGCGTGGATGAGTACAGTA200.10218157666172789No Hit
GCGAGATGTCTGACCGGGTGTTTATTGCGGATTACATAAAAAAGAAGCGA200.10218157666172789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)