FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-heart-RNA-8_S58_L008_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-8_S58_L008_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18617
Sequences flagged as poor quality0
Sequence length32-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.22560025782886609No Hit
AGAGGCTGGAGCGTAGGATCGAGATGTTTGAGCTAGAGAGAGACGAACAA350.1880002148573884No Hit
AGAAAAGAACCCCATGCGGGATCTCCGCATTGTAAAACTGTGTCTTAACA350.1880002148573884No Hit
GTCGCGTCGAGGTGAAATATAACGGTGTCTGGGGAACAGTATGTGACGAC340.18262878014717732No Hit
GAATCATCGCCCAGCCACAGTCTCCATGATGATTGATATACAACATTGCA320.1718859107267551No Hit
GAAAAGTTATATTGTGGTTAAACAAAAATATCATGCTACAATGATTATGT320.1718859107267551No Hit
GCAAAGACAGGCCTCGGCTATGCTGTCGATCATAAAATGGAGAACAATTA320.1718859107267551No Hit
CTACACATATGTTTGTTTGATGGATATATGTATGACGGGTTTGTTTATAA320.1718859107267551No Hit
TGAACATATTATATGCTGAAGCATTTTGCTCGAATGTGTTCGCTCAAACT320.1718859107267551No Hit
GTGAAGCTCCTTCAGAGCCGAGGAATCCTTCCTGGAATCAAGGTTGACAA320.1718859107267551No Hit
TGTTGATTCACTAAACTGAACTAAATAAAACAATATCATCATAAAGTTTG310.16651447601654404No Hit
AACTTTTCAATGTACGAAAAATAAAGTAACGACGTGATAACATGTTTGAG310.16651447601654404No Hit
GCAGATTACAACTAAGATCACGAAGGGATGCATTGAAATTCTTAATGATG310.16651447601654404No Hit
GACGAATCAAGATTGTCGTATATCTATCCAATATTTGGAACTATCTGTAT300.16114304130633292No Hit
TGCAGGTTTTCTTGAACCAATCTAAATGCTGTACATGTTGAATAATAGAT300.16114304130633292No Hit
CCCAACTCCAAAAGCTAAACCAACGCGTAAAGTGCGCGACGAGCTACCAG300.16114304130633292No Hit
ACGAGTTGTGGTCGTGAGATGTGAAGGTATCAACATTTCTGGAAACTTCT300.16114304130633292No Hit
CTGCTAGGTGTGGTGGGTTAAGAATAGCTGGGTCTTTAATCAGTTGAATA290.15577160659612183No Hit
TCAGCTCTCCCATACAGAAGATCTGTCCCAACTTGGTTGAAGCTTACATC290.15577160659612183No Hit
GTAATTTACAATTATGACAGATGGGCGTTGTTTGTGACGTCGTCAAATTA290.15577160659612183No Hit
CTTTTTGTGACTGTGGGTCCATATTACTTCCGTTGGACCGTAGTTTTTCT290.15577160659612183No Hit
CTTAACAAGATGATTCTAGTCTCATTTTGCCCGTCATTTTGTTTTAAATG290.15577160659612183No Hit
AAACCCTTATTAAATATATTTGTGTAATGTACATAAAATGTAAAAGTTAC290.15577160659612183No Hit
CGAAAATTGGCAACATAAAAGAAATACGAAAGAATGTCTCTTAAATCACT290.15577160659612183No Hit
CTTTACTTTATCAGAATTTGCGAAAACAACTTAATTCTATAATCACGTTC280.15040017188591073No Hit
GGCAAATTCAAAGTCCCATATGCCAGCTTACACGGGAGAAATTACCAAGA280.15040017188591073No Hit
GCAAATGATGCCAAGTTGAAGGAGATCGTCACATCATTCATTGCTTCATG280.15040017188591073No Hit
CCAACGCAAATTTTCATCTACACGTTCATGAGTTCGGAGACACTACCGGT270.1450287371756996No Hit
TGTGAATATACGGCCAGAAATTATAGAGTTAAAGCGAGTTAGCTTATTTG270.1450287371756996No Hit
CGGGAAGAAATGTCAAACTCCCTGAAGATCGTGAATATTGAGGAGATTCC260.13965730246548852No Hit
CGCCATTCTAGTCTATGATATTACGGACGAAGATTCATTCCAGAAGGTGA260.13965730246548852No Hit
CCGGCCCATCCATCGTCCACAGGAAATGCTTCTAAGTTATTCGATCTATT260.13965730246548852No Hit
AGAAGTCTCCATCAACCTTAATACATTGGCAGCCAAACAGCAAAGTATTG260.13965730246548852No Hit
GCAGTATCGTCCTTATCTTATTTCGGCAACTTTGTAATAAACGGCATATC260.13965730246548852No Hit
TGAAAGCCAAAATTCAAGACAAAGAAGGAATTCCCCCAGATCAGCAGAGA260.13965730246548852No Hit
TTGGTTCTTGATAAGAATAAAATAACTGTCAGTGTCTGCAGAGGGGTAGG260.13965730246548852No Hit
TGTTTTTACACTACTGCAACTCTTGTGGCCCAAACTACAGTTGTGAAGTG260.13965730246548852No Hit
CTTCTTTCCGCTTCAAATTCATTGTTAAACGCCTCTTTTAAAAGAGGAAT250.13428586775527743No Hit
GTTAGCGCAGTCTGTAGAGCATCTTATCAATGCAGGGATTGCAAGTTTTA250.13428586775527743No Hit
CAGGCGTCAGAGAAACGTGTTGACCTCGTGAAGCATGTCTGCTCCAACAC250.13428586775527743No Hit
CCAGGTATTTTGGAGACAGTGTTACTTTTAGGGACAAAAACTAGTTACTT250.13428586775527743No Hit
GGTGAATTATGTTTATGTTAACTGACAGAGTGGAAAACAGTTCAGGAAGT250.13428586775527743No Hit
GTTTGTTGCTGGTTTGATTGTACTGCTATTGGATGAGTTGTTACAGAAGG250.13428586775527743No Hit
CTCATCTTCTCTTGAGAAGAGCTACGAACTCCCCGACGGTCAAGTCATCA250.13428586775527743No Hit
GTTTTACCCAATCAAACCAATGAGACGTATACCAATAAAATGTGCCAAGT250.13428586775527743No Hit
TTTATAAATCAGATGTCGGAAGACCAATTCTTCCAGTGGTTGATGAAAAG250.13428586775527743No Hit
CAACACTGAGTATTGGTTCTGTGCAGGAAACAACACTGAGTATTGGTTCT250.13428586775527743No Hit
AATCTGATCAATTACCTTAAAGTGTAGTTTTGAATCACATTTAAAACCTA250.13428586775527743No Hit
TCGCCTTCCAGGCCGATGAGGATAGAAAGAACCAGGAACGTCTCCAGGAC250.13428586775527743No Hit
GTTGGAAGAACTTGTTTCTTAATTAACATAAAATATAAAGTTTAAACTGT250.13428586775527743No Hit
AATTCCTTGTGTTATAACTAGAAAGACAATAGTACTACGCAAGTTACACA250.13428586775527743No Hit
CTTGACCAGGTATAATCTGCACATACTACTATGTATTATTATATACATGT250.13428586775527743No Hit
TGAAATTTCACAAAATCATGGGTTTGTAATGAAGGAGACAAACTTTTAAA250.13428586775527743No Hit
CGTACCCGAGATGAAAGTTGGTGCAATCAATTCTAAAAAAAAAATAATGA240.12891443304506633No Hit
TAGTTACAAACTCATCACACCATCTGTTGTGTCTGAAAGACTGAAGGTCC240.12891443304506633No Hit
ATAAATTCTTTATGTATATTGTTATTGGATGTGATATTGCTTTTTACAAA240.12891443304506633No Hit
TGAATGTCCATGTTACAGAATATCATGTCCCCATAATTATGAATGTCCAT240.12891443304506633No Hit
TAGACTCATGTGATAGTTTAGGACAATATAGTGTTACTATCAACTAGTGT240.12891443304506633No Hit
GTGAAGTAAAAATATTGTAAATGCCCACTGCAAAATTTGAAAAGTCTGTG240.12891443304506633No Hit
GGCGAGCTTATTTTAGTGCGGCAACTATGATTATTGCTATCCCTACAGGA240.12891443304506633No Hit
GGCAAAAACGCTATTTTCTAATAGTTCTACTGTCAGTAGAAATTATGGTA240.12891443304506633No Hit
CGTGTTTACAGGAAGGAGCTTTCATTCACGGACGTCAAAATTGTTAACCT240.12891443304506633No Hit
TGATTGTTTAAGATAAGTGGCTGCTCAATAGAGGTGGTCGCTTACACAGG240.12891443304506633No Hit
TTCTGCTCCTGATATGGCATTCCCTCGAATAAATAACATGAGGTTTTGGC240.12891443304506633No Hit
GTAAGGCATAGATGGATTTTGTTGATACTTAGTACAAATGATCAACATAA240.12891443304506633No Hit
CACGGAACGAGGATTTCATCTTCTGAAAAGTGAGTTTAAACTGAAACAGA240.12891443304506633No Hit
AACGACATCAGAATGGCGTGGTCAGGTATGGCCGGCAGGATTCCACAATG230.12354299833485524No Hit
TGTAAATACATCTTGGTCCGTAAAAGTTCTACACTATTTTGATATTATGT230.12354299833485524No Hit
GGAATGTTGTTATTAAGGGTGTTGGGGTTCTTTGTGTTGAGTTTATGCTT230.12354299833485524No Hit
TGAGAATAATCTTTGCCCTTCTATAAGTAATTGCCAAGTATTCTAACATT230.12354299833485524No Hit
GTTTTAATAAAAAAAAATATCCAAGAATAATGGAGTGCATGGTGTATTGA230.12354299833485524No Hit
CGGTAGTTCATCTTATTTTTCTTCATCAGACAGGGTCAAACAATCCTTTG230.12354299833485524No Hit
CTGACTTCCCAAAATATTATAAAGGACGTTAAATTGTTTATTTTCCTGAG230.12354299833485524No Hit
CTTCCATGGCATGGACCTCACCCGTGACAAGCTCTGCTCCATGGTAAAGA230.12354299833485524No Hit
GTTCGGGTCCGAATGCAAACACATGTGTCATTGCAAGGACGCCAGAGAAC230.12354299833485524No Hit
TTGAGTGCTGCCATATTGGATTTGCTGTTCTGTCATACATGTGTATTATT230.12354299833485524No Hit
GCCATCCAGGCCGTACTCTCCCTGTACGCTTCTGGTCGTACCACTGGTAT230.12354299833485524No Hit
TGGTTATGGTGTGTATACTTTTGTACACAAACTTTACATATGCCTCTTGT230.12354299833485524No Hit
GTTAACATGTTTTGTTTCAATACTCTTCTTCTAACACAGATTGTTACCAT230.12354299833485524No Hit
GTTTGTCGCGCCTTGGGCATTCCAACACGTAGCGTTACAAACTTTGAATC230.12354299833485524No Hit
GCATATATATTTATAAAACATTATTTATTTTATATTTATCTCATGTCGAT220.11817156362464415No Hit
GTTCGAGCAGACACCATCCTGACTGGCGAAATGAAGAACTTGACAGAGAA220.11817156362464415No Hit
GACTGGGCCTCCTGTGAATCTAGCTGGGTCTATGAATAATAACAGTTAAT220.11817156362464415No Hit
GTTTTATATCTGTCTTTGCTGATTCTGGCTGCATGTTCGATACACTATAC220.11817156362464415No Hit
AGGAAATGTTGTTAAAGATTTGACAGGTAGAGTAGTTAAATGTTTACCTT220.11817156362464415No Hit
TGCGTAGCAGTTTAATTAGTATAGACTGTTTCTGCATTCACACAATTTCC220.11817156362464415No Hit
GTCTTATATACAGTCAAACCTGTGTAAGTGGTCACCTCTATTAAGCAGCC220.11817156362464415No Hit
GTTTATTGCAGTATTCGTTCTTGTACAATATTTGACCTCCGTATTTAGTT220.11817156362464415No Hit
TTCGCAGGTCGTCAGTAGTAACTGAACTACCTGCCGAGTCCATTTGGCGG220.11817156362464415No Hit
TTCAAAGTTCCGTACCCGAAATCATTCATAGAAACTTATATCTCATTTTT220.11817156362464415No Hit
CAGAACATGGGCAATGTGCTACAGCTGTGTATGATTACCAGGCTGCTGAT220.11817156362464415No Hit
GGAGCACATGTCAAGGTATGTTTGTACAGTTGAAACTGATGATGAAGGAG220.11817156362464415No Hit
GATGTATACTTTATAGAATAGATCAAGACAGTACGGAGCTTTTGTCAGAA220.11817156362464415No Hit
GTGTAAACCACCAAACACGAACTTTACATTTGGTGGCGTTTACCAGAAGT220.11817156362464415No Hit
GGTGGTTAGAAAGATAATATTTAGACTTGCTTTAAGATTTTGGGGGGGAA220.11817156362464415No Hit
AAATTGCTGCCATAGCACAGGAATTAAACACATTAGAATATCACGACATA220.11817156362464415No Hit
GGTGAGTGATTCGTAATTGCCATGTTAATGGGGCTTCGTTGTTTTTTAGT210.11280012891443304No Hit
AATGCATTTTGTCTATGGTATTTATTTCTCTATCTAATTACGATACATAT210.11280012891443304No Hit
AACAGCAGTAGTATTCTGAGCAACTGTAGTAGTTACATCTGTACTGTGTT210.11280012891443304No Hit
ACTCCATCCTGACCACCCATACCACCCTGGAGCACTCCGACTGCGCCTTT210.11280012891443304No Hit
GTTGACTGTGTAGAACTAACCATAAAGACATTGGAGTCTTATATTTTATC210.11280012891443304No Hit
ATACCTTTTTCACATCCTTTAGGATAAATAAATAAATATGTGTGACGACG210.11280012891443304No Hit
CATGGATCTCATTGCCAAACTGCCAACCGTTGCTGCCATCATTTACCGTA210.11280012891443304No Hit
GAGAAATATTACACACACATGCTGCTTTGTCACAGATTTTAAATTAAATT210.11280012891443304No Hit
TGAACACATTGCTGTGAAAGCAACAATATTTAACTACGGCAATCCCGGAG210.11280012891443304No Hit
CCAGACGCTACATGGAGCGCCAAGCTGATTAGTGGAAAGAGGGTACCAGT210.11280012891443304No Hit
GCAAGATTAGCGGATAATCTTTCTAAACCGAAGATAATTTTGGCTGTGAA210.11280012891443304No Hit
TTAATGAGGAAAAATCATATTTAAATTTTATAGTGAAAACCTCATTGTTT210.11280012891443304No Hit
GCAAGATCGTGAAAACCAGTCTATGTTGATCACCGGGGAGAGTGGTGCAG210.11280012891443304No Hit
ATGCTATTGTTTGGACTGACCAATACTACGATTCTTGTAAAAAGTTGTTG210.11280012891443304No Hit
TTTTAATTGCTATAGGTATGTCTTTTGAAACAGTTTTCGGTTGTTCTTTA210.11280012891443304No Hit
AGATATAAGTCCTTGACCCTTAACTGCTAGGTGTGGTGGGTTAAGAATAG200.10742869420422195No Hit
GTGTGCTCATGCATACATGTGACATTATAAAGGTGTTACTATATGTACAG200.10742869420422195No Hit
TGGGGGTATGAATAAATTATCTCTGTGGTCTGCATCATTTTGTCAACAAT200.10742869420422195No Hit
GGGAAAACTGCCATAAATAAAATGATTATTAAAATGGATAAAAGCTATCT200.10742869420422195No Hit
TGTTGAATAAACTTTTTAATGAGTTTGTTAAAGAATACCAATCTTTTTCG200.10742869420422195No Hit
CTAATACAAGTAATGAACACAAGGTTCAAAGTAAACTCCAGAACATTAGT200.10742869420422195No Hit
GCCATCACGAACACTTTATACCTCCGACACGAGCGTGGATGAGTACAGTA200.10742869420422195No Hit
GCGAGATGTCTGACCGGGTGTTTATTGCGGATTACATAAAAAAGAAGCGA200.10742869420422195No Hit
CGGAAAGAGGCATAACAAAACCCACACACAGTGTCGGCGATGTGGGCGCC200.10742869420422195No Hit
CGAGAGGATTGACAAAGAACCAGGACCATGGAAAAATTATACAGACCTCT200.10742869420422195No Hit
AAAACGACTGACACACGTCTTCGTAAACCTCATCTCACCATTAAGGCGCC200.10742869420422195No Hit
TTAAAATGGCATTGTAGCAGAAAAAATTCCCGGCAAAGGAAAAAATGTTT200.10742869420422195No Hit
GGACGCCTTCATTTCGTTAGTTCTTGTGGCTTATTTTCAGTTGAGTTTAA190.10205725949401084No Hit
AAAAGATTCCATTGGTTGGCGCCTTTAAAAAGATTCCAATGGTTGGCGCC190.10205725949401084No Hit
GCCACCCTGAACTTATTAAAATGAGAAGGTATAAAAGATTGTAAACTCTT190.10205725949401084No Hit
ATTTAAGAACCAGTTTCAGTGGTTCAAATAATATGATAACACTTTAATAG190.10205725949401084No Hit
CTGAAATAACTCATCGGTCTGGCGTATTTGTTGGTGCTGGCGGCAATCCT190.10205725949401084No Hit
TGACTGTCTCATCTATAAATTAAGAAGTTTTCGTTTAAGTGAAAAGTCTT190.10205725949401084No Hit
CGAGAATGATTTTATTTATATTTTTTTAATTTATATAATGCTGTTATTGT190.10205725949401084No Hit
CAACACAACTCATGGAAGACAACAGCACCCTCAATACACAACCACAGATC190.10205725949401084No Hit
TTGGTATCAACATTAGGATTAAAATTGAAAATGAAAAAAATATTTAAAAT190.10205725949401084No Hit
AAACTGACCACAGCTGGGTTATTTGTTAACAAATATATAATGTGTATATT190.10205725949401084No Hit
CTATCATAGACACTGATCTATTATAGACACTAATCTATCATAGACACTTA190.10205725949401084No Hit
ATTGATGTTAAACCCCATTATAAAAAAAAAGATTGAAAAAAATACAACAA190.10205725949401084No Hit
CTTGTGTAAGGAACATAGGAACAATGTCGACCGGCTGTAGGATTTTGGTC190.10205725949401084No Hit
TGGCCATGATGACTGTCATATAGGAATGTTCAAAGATTTCCAGAGGAAGT190.10205725949401084No Hit
TAGTTCCTCCTACCTCCCAAGGGATCCGATTTTTATTGCATTTTTATTTT190.10205725949401084No Hit
CCAACTTTCTCTTTTGTCCGCCAAGAGATTGCACTTGAGCAGAAAGCTCA190.10205725949401084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)