FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-heart-RNA-8_S58_L008_R1_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-8_S58_L008_R1_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18617
Sequences flagged as poor quality0
Sequence length32-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.19874308427781062No Hit
CTCCTCTGCCTTGGCACCTCTAACTGTGCAGTGAACAGCAATCTTTTCAT340.18262878014717732No Hit
GGAAGCGATCTTCAGCACACATCTCCACTTGGCAAACTGGGCGCCATCTT340.18262878014717732No Hit
GCCGCCTGCCCAGAGAAGCTGTATCCAATATATGATCCGGTGATCTTTAA320.1718859107267551No Hit
GCCGTGTTCACAGTCATGAATTCCGAATTTTCTTCTCGGACAATCAAAAA320.1718859107267551No Hit
CACGCTTGCAAATTTTGCTCTTTAAAAAAACGGTCTCCAAGTGTGTTTTA320.1718859107267551No Hit
GCCAAAGATCCTAACCTAAAATGAAAAAAGGACCAAAAAAATGAGACAAA310.16651447601654404No Hit
GTTTTTGTATCCGTAGATTCTCCTCCATATCTCGTTTATGTTGTTCGCGA310.16651447601654404No Hit
GGCGCTTCGTGGGACTCATCCTCTACTTTTGGCACATTATCTGAAGTGTT310.16651447601654404No Hit
GGGGGTACCCCCTCAAACACCTTCAATCGGGAAATGGCCTCCTTACCACG300.16114304130633292No Hit
CAGACCCATCTGCAATACTAAATTGAGAATGGCACCTTCTGAGCTGGTAT300.16114304130633292No Hit
GGACACTCCTACAGGGGACTATTTTCATTGTTGAACAAAATGCTAATTAG290.15577160659612183No Hit
GTCGTTCACAACCATAGCATCCTTAGAAGTTCGCCGGTATCTCCCCACAG290.15577160659612183No Hit
CAGCATTTGGCTAATTTCTCATTTTTCTTACAAATAACATTTTAATATTT290.15577160659612183No Hit
GCTTTAAGGAGCTTGTTTATTAATTTGCATATTGCTACTCCGTCTCTTAA290.15577160659612183No Hit
TATAATGGAGAATTGAATTAAAAGAGTAGAATGTTTATGGCTATGTTTTT290.15577160659612183No Hit
GATAATGACAGTTATATAATAACCATAAAGTTAAAATATTAAAATAACAG280.15040017188591073No Hit
ATACACTCTCTCAATTACCAGACCATATGAGAAGGGCGAGATATTCAACA280.15040017188591073No Hit
GCAGTATCGTCCTTATCTTATTTCGGCAACTTTGTAATAAACGGCATATC280.15040017188591073No Hit
GATGCAAGACCTTTACTCTTCAAGATTCTCAAAATCTTGTTTCCAGTAAC280.15040017188591073No Hit
TTCTGGTTCAGGAACAGTTTCCGGTTCTGGTTCAGGTACAGGTTCCGGTT280.15040017188591073No Hit
GTACACATTTATTAGATCAACTATTGAACAATACAAACACCATATTTTTA270.1450287371756996No Hit
GGAACGCTTAGTTAGTCCACATGCCTGTTTCAAACTATTACATACCTTGA270.1450287371756996No Hit
GCACTAATGTCTTCATTCAATCACACATGGATCAATAAACCTTTATAAAA270.1450287371756996No Hit
AGAAGATACCCCCGCCAGATGAAGAGAAAAAATCCCATAATCAACAGAGC270.1450287371756996No Hit
CAAAAAATGAGGTATTAAAATTACGATCTGTTAATAATATTGTAATAGCC260.13965730246548852No Hit
CAAAACACCTACATTTAAATGTTACAGATTTCATTATGCTTTATCTTACA260.13965730246548852No Hit
GTAATTTACAATTATGACAGATGGGCGTTGTTTGTGACGTCGTCAAATTA260.13965730246548852No Hit
ATATGCCTCAGCCTCCGCCACAGTTACATGTCGGTCTTTTTCCATATCTA260.13965730246548852No Hit
ATTTTTGCAAAATAAATAAGTTAACAAAGTTAACATGTAAATAATTAAAA250.13428586775527743No Hit
CAAATACAATTTTCTTGAACGGTGATAATTTTATATGCTATAAGATAATA250.13428586775527743No Hit
CAACACATTGCAGTTTATCAATGCCAAAAAGAAGGAAGATTCCAGGTTCA250.13428586775527743No Hit
CTAAACTTTACTTTATTACTTTCTTAATAAAAATATATAAGCTCGACAGG250.13428586775527743No Hit
CTACCTTTCCGTCATCGTCAGAAGTAACTGGACCAAGATCTCCAACGTCA250.13428586775527743No Hit
TATATATTATATACCGTCTATCACTTCATTAGAGAATATTGTGCGAAAAA250.13428586775527743No Hit
ATTTTTTATGTTTTTATTCTGTTTATATTCATTTATAACTACAATCATTT250.13428586775527743No Hit
CTAAAGCCTATATTCATAACTGTGTTTCAAGTCTAAAGCCTATATTTATA250.13428586775527743No Hit
GAAACAACAACAACAACAACAACGTCTAATGAATTGTAGAATACTCAAGG250.13428586775527743No Hit
CAAACCCATCTCTGCAAATAACTGATAACTTTCCCACTTTAATCAGTGGT250.13428586775527743No Hit
TTTGACTGTATACTTTTGGCGGTGGCATATAACACATACGTGTACCATTT250.13428586775527743No Hit
AGAAGCTTACTAACTTGTCTTGAAATTATCAATTCCAAATATTCGACAAA240.12891443304506633No Hit
GTCCAAACTTCAGCACAGCTCGACCAGTCAGTCTACACTCTGCCAATTCT240.12891443304506633No Hit
CATCAGCAGCCTGGTAATCATACACAGCTGTAGCACATTGCCCATGTTCT240.12891443304506633No Hit
GGTGCTTTCACGATGTTCAACAAGGAGGGTCAGGCAAGTCACGATGAGTA240.12891443304506633No Hit
CCGCCCTGCGCTGTGATGTCAGCGTTCCCAGTCTATCCCACTGATCACAT240.12891443304506633No Hit
TTTTTTTATTTGTATTTTACAAAGAATCACTCATCCTCAGCCTTTGTGGC240.12891443304506633No Hit
TCTGGGTCCATCAGCCATATAGGAAATAAAACAGATAAGAAAAAAATAAC240.12891443304506633No Hit
TTATTTTACATTGATTGTGATACAAGTTCTTGCAATGTACAATTCTTATT240.12891443304506633No Hit
AAAACAAACTTCACTTTTGACAATTGGATACTTTTGGAAATACATATATC230.12354299833485524No Hit
GCATTATCTTCCACTAAATATATGGACAAATTTCCACATATAACACTATT230.12354299833485524No Hit
CTTGGTGTTGTTTCCTGCATGGAACATCGAACCAATACTCGGTGTTGTTT230.12354299833485524No Hit
CAGGGCTGAAAGCCTTCCATACGATGGTCTCACAAATGTTGGTGGCAATA230.12354299833485524No Hit
CGAACATACCTCACCATGTGCTCCTTCACCATCAGTTTCCACCGTACAAA230.12354299833485524No Hit
CCACCTGACCAGCAAGATGACATACCCCAATAGAATGATCGAATAATGTA230.12354299833485524No Hit
TCAAGTAATCTGTAAGATCTCTGCCAGCCAAGTCAAGACGGAGGATGGCG230.12354299833485524No Hit
CAAGAAATAACATTAAATATAAAACAGTGAAGTTAAGAAAGCAAATAAAT230.12354299833485524No Hit
TGACCTTTGAGGTATAGGTGTGGGTCTTACTCGCGACACATCGTTATGTT230.12354299833485524No Hit
CAGAATATTTCTTTGCATTGAGACCACGTGATTCGATCTTACAGCTTTGC230.12354299833485524No Hit
CTAGTTGCTGCCCTTTACACTTCCTTCTCTTCGAACTACTTAATTATATT230.12354299833485524No Hit
AAAAACATTTTCTATTAAACATGTAAATCTATCACAATATTATATGCAAT230.12354299833485524No Hit
TTATGAATTATATTTTATTGAAAAGCTAAAACATAATCAAAATTACCTTT230.12354299833485524No Hit
CTCTTTCTCCAATCGCAATACATTTCAACAAGTGTTATCCAAACCCGAAA220.11817156362464415No Hit
GGAAACCAAAAGTGGAAAGGAAAGAGTCCTAATTTTAGAAACAACCCTCT220.11817156362464415No Hit
CAGGATATTCTATAAGATGGACATTCATTATTATGATGACAGGGCATTCT220.11817156362464415No Hit
TAAAGTTCCTTTTGTACAGCATCGACTACAGCTAAACCGTAATTAGTCAC220.11817156362464415No Hit
TTTTAATGTAAGTTTTGATATTGACGTTTGCACTGCACATAACAATCAAG220.11817156362464415No Hit
CAAGGTTTTCCCGGGGCATTTCATCATATTTGCCAATGAGTTTGATAAAT220.11817156362464415No Hit
TTTACACAGATGACAAACAATACATTATCTACCCAAGCCAACAATTCTGA220.11817156362464415No Hit
GAAGTTTCTGGAGGGAGTTCTCAGCAGAGTCGGCACGCTCCTCTGCATCT220.11817156362464415No Hit
CGACACTCATCTGGACACTTTTATTTTATAAAACATAAATATCTATTTTA220.11817156362464415No Hit
TTATTATATCGGTGTGAAAACATTTACTGGCCACCACGTAAACGAAGTAC220.11817156362464415No Hit
TACACAACCAGGTAGTACAACAAACATAAGAATCAGATAAAATATGGCAA210.11280012891443304No Hit
GTTTTCCCGCACCAATCACAGAAGGCCTTTGACAACAGTGCAGGAACAGG210.11280012891443304No Hit
TGGATAGTAAGAAACAATGGTTAATGAATGGATTGGATTGATAAACAGTA210.11280012891443304No Hit
AGGACCGATTCATATCCGCTGGGGCAACTGAAGGCACCAGTCATGGGGTT210.11280012891443304No Hit
TTCTAAATGAAATTTGACACACGGTAAAGCAGCACAACTGATAATGTTAT210.11280012891443304No Hit
GCAATTCTTTAGCTACTTAGCAGAAGCTATATGTGGGATATCGAAAAATT210.11280012891443304No Hit
GCCAGCTTCTGGCATCTTTTTAAGTCTCTTCTCCACATCGCTCGTCGTGC210.11280012891443304No Hit
GCGTATCAAAACATATAAAGCACTTTCACTGAAAATCACAAATATCCAAC210.11280012891443304No Hit
ATCAAACAATTGTATACAAACAGTTCTGACTTCTATTTCTTATCTAGTGT210.11280012891443304No Hit
CCTGTTTTAAGAGCCAGTCAAGAGAAGGAGCAAAATTGAACTTTTAAGAC210.11280012891443304No Hit
AATTTCTAATTTCCTATCAATATAAGATCTAATTACAAAGTGAAACAATC210.11280012891443304No Hit
GAAGACACTTGTATATCTTAATCCAGTGTTAAGCACAACGCTGTTCTATC210.11280012891443304No Hit
TAATCTTTTGATACACAATTTCAACCGCAGCTCAATTTATTTTTTAAATT210.11280012891443304No Hit
TTCAAGTTTCATTTCACATGAAACGATCAGCTAAACTGATTGAAACCAAT210.11280012891443304No Hit
GGACAGTGTTGGCGTACAGATCCTTACGGATGTCGACGTCGCACTTCATG210.11280012891443304No Hit
AAGGACTCTTTGGAGTGATTGTCTTGTCGGCCTCGAGAGGTTTGGAGGCG210.11280012891443304No Hit
TGATCATTAAATGGTGCTGTGACCGCGAAATACTGCCAGTCGTCTTTATA210.11280012891443304No Hit
TTTTCCACCCAGTCTGAGTGCAACTGTTTTTGGTCTAAATCCAGAGTACC200.10742869420422195No Hit
TTTTAAAGTTCAATGAAATTTTAATTTATATAAAGGTCTGTTTTGGCAAT200.10742869420422195No Hit
GTGAAAGTCGTCAATCAGTATTTAAAAACTTTCTATTTTTAGAATTATTT200.10742869420422195No Hit
CTTGGCATAGTCTTGGCATATATGTTGTGTCTCGTGTTTTATTTAGTAAT200.10742869420422195No Hit
CGACAATATTCATCAAATGCAATATCTCTGTATTGACACCCAGGTCCCAT200.10742869420422195No Hit
CCACATTCCTCTTCACGGTCGTAATTTTGTTCCATCTTCATTTCGACTTC200.10742869420422195No Hit
CACAATACAGCAGACAATAAGCTTTAAGCTACAAAATTATACAACACGCC200.10742869420422195No Hit
GGGAGATATACCCTTGACTATGTGATACAATTGGAATTGGGTTACCAGGA200.10742869420422195No Hit
CCCATTTTTAAGAAAAATTTGGATGATAATCTAGCAATTAATTTATAATG200.10742869420422195No Hit
TTTTGCGTCTAGGATTAAATAATCCTCTCATGTTGAAGCATTAAAAATAT200.10742869420422195No Hit
CGACGCGTCAAGGATGTTCTCCTCTGGTTCCCAAGTGCAATGCTTTGAAG200.10742869420422195No Hit
CAGCACGTGGACCAATGTCAAACATCTTGATAACATCATTGACAAATTGT190.10205725949401084No Hit
AGCACATCTACTCTTTGATAATAGTAAAATTGTCAATTTAAGTTCCACAA190.10205725949401084No Hit
AAGAGTTTCACATTCTCAGGGGCATAGCTTCCTAAAGGCATGTGCCTACA190.10205725949401084No Hit
AAAATTCAGTTCATTTTTCTTGTTTAGATATAAACTATTATGGTAATTCA190.10205725949401084No Hit
CAAGTTTGCAACGCAAAAGGGCATGTCGTTCGGTTCTGTACGTCACGGAG190.10205725949401084No Hit
CTAACTGCTCTTCAAGGAAACAAAGAATGAATATTAAGGACTAAGAAATG190.10205725949401084No Hit
TGTGCTTCTGCTTCATCAAGACGTACGCCGAGTTCCTTCATCTGCGCCTC190.10205725949401084No Hit
AAAGTTGTCAATTGGAAGCAATTGTCAGAATAAAATATTTTGATACCACA190.10205725949401084No Hit
AAAAGGTTAAGATAACCGATACATCTAAGAAAGCGGACTAATCTCATCAA190.10205725949401084No Hit
TTTTATCTGTTCATATATGTCAACTACAATTAATTTGTTAAATTTGTGAT190.10205725949401084No Hit
TCAAGCTACTATAGTTAACCCTAACAAACTAAATGACACTAAAAACATCC190.10205725949401084No Hit
CTTCCTGCGCTTAGCCTTAACACTCCAGTTAAAGTGTCTGCCGGTTGCAC190.10205725949401084No Hit
TGCAAAAATATGCATGTAACATGTTTTAGTTAAATTAAAAACTGTAATAT190.10205725949401084No Hit
TGCTAACTCTATTCGAATTAATATTCTAAAAGCTGTACCAACTATACCAG190.10205725949401084No Hit
CAATGACAAAGGAAGTAAAGTTAAAACCCGTCAACACATAATGAATAGAC190.10205725949401084No Hit
CAAAGACCAAACTAGCGTGCTGACTTTTCCTTTGGATTCTGTTTAAAGGC190.10205725949401084No Hit
GCCAATCAAAGATCAGTGTCTATGGTAGACTAGTGTCTATGGTTGAATAT190.10205725949401084No Hit
ACGAAGAGCTAATTATGAGTCAACTGAGGAGCCCCGCACTCAAAAAGATT190.10205725949401084No Hit
CGTTCACAGTCTTTAATCTCCAGATTGGACTTCTTTGGCGCAGTAGTTAT190.10205725949401084No Hit
AATTGCTTGCCAGCCGCCATAACCTAAAGGAAGATCAGGCCGTGTCATCC190.10205725949401084No Hit
GGCAAAGGTCCTGCAGATTTAATCAACGCCCCTTTAAAAGACGAGCAAAC190.10205725949401084No Hit
ACCCTAAAGCCCAGTACATTCTTGGCCTTAGTTGTACATTCCTTCCTCTG190.10205725949401084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)