FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-heart-RNA-6_S42_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-6_S42_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC532.543186180422265No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT140.6717850287907869TruSeq Adapter, Index 13 (97% over 40bp)
CTCTGGGTCCATCAGCCATATAGGAAATAAAACAGATAAGAAAAAAATAA110.527831094049904No Hit
AGTTTAGGTTTATGTACATCAGTGGTCTGTGTACAGTAATGGTTTATGAA110.527831094049904No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG110.527831094049904TruSeq Adapter, Index 13 (97% over 39bp)
GAAGCCTCCGCTTTCACGACACCCTCCTGGAATTGTTCGTTGACTTTGTC90.43186180422264875No Hit
TGATGGGAGGATGCTAACAAGAGAGTCAGGCTGAGAGTCAGCATTGATGC80.3838771593090211No Hit
CATTACTTACGACTTCAATTCACACCAATCCCATCGCAGCCCCTGACGGA80.3838771593090211No Hit
GCCATAGTAACCATAACCAACCTTAAGAAGGTCCTGTGCACCTATTGATT80.3838771593090211No Hit
AGACTTTATTGCAATGCAATTATTATTTACAAAATAAAAGACTTCATACA80.3838771593090211No Hit
AAAGTTTGCAAACTAAAAAAAAAATTTTTGAGTCTCTAGCGGATAAAATG70.33589251439539347No Hit
GCAACGTGCAACTTCATTCCTTTAAGTCTGTATGGTTGAGCGGTTTTCAT70.33589251439539347No Hit
CAAGCCCGCCCCCATACAGACAACCAAACAGACAAGTGATAAAGGTAAGA70.33589251439539347No Hit
TTAGAAGCCCGTGACCATCTTGAATCGCGTCGTCTGCTCGAATGTCCGAT60.28790786948176583No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.28790786948176583No Hit
TGCAACCTTAATTCTTTCATTGAGGAATTTCTCAAAGCTTGCAACATCCA60.28790786948176583No Hit
CAATGTCATGAGATGATTATTTATTCACTTTTAACACCTAGGAAGGTAAT60.28790786948176583No Hit
TCAAGTAGGCAGAGTAGAAACCCACACCAAACTGTCCGATCATTGAAATG60.28790786948176583No Hit
GTTTGGTAACTTTGGGTTAGAAGACGGCTTGATCATCATTTCAGCGAATT60.28790786948176583No Hit
CCTTCTCTTTCTTCCCTCTCCTTCTCTTTAAGGAGCTTATCCTCGCGGAG60.28790786948176583No Hit
TTTTGTTCATTCATATTTTATTTTTCACTATCTGGTATTGGCATGATGAT60.28790786948176583No Hit
GATCACCATGTTTTACTGTTCATGAATGTTTGTACTTCGGTGAACACGTC50.23992322456813817No Hit
CAGAGTTTCAGCAAATTCTATTGCATTACTCAAATTGTCGCTTCTTACGA50.23992322456813817No Hit
CGGTTTCCATCCTACGCTTGTCGTTTGTGAGTGACGTGACCATGAGTGTG50.23992322456813817No Hit
ATGTAAAATTACATCTAAAGAAGCACTAGAAAGAACAATCCCAGTTAAGC50.23992322456813817No Hit
TTCCAATCTTGTGTGCCTTTCACAATATCATCACCAATTTTCTTTGGGGA50.23992322456813817No Hit
GAGGAAAGGCTTGATCTTAGACCTGTCCTTCATCTTTTTCTTGCCCATGC50.23992322456813817No Hit
TTTTGCTGCGCATGAAAAGGCCACTTTATTTCTTGTGTACAACTCTCACT50.23992322456813817No Hit
CCCCCCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC50.23992322456813817No Hit
CTGGACCTTCTCCAAACTCCAAATTTTGTACAACAAAAGTAAAATGGTAC50.23992322456813817No Hit
CTTTGTTTAATGTTATTCTAATATATTGTAACTATTCGTCCGCATCTGAC50.23992322456813817No Hit
CAAGCATAGAATGCAGTAAGTATTGTAGGAAAGAGAATTATAAATAACCC50.23992322456813817No Hit
CTTGTGGGCTTTGGCAAGATTCTCCTCTGTGCAATGAAGACAACATGCTT50.23992322456813817No Hit
ATAAAGGTTTTCAAGTTACTTTTTGGATAACCATTGTACGGTATGAAAAC50.23992322456813817No Hit
GCAACCTTCAGCAGATTTAAATGTGCAACATTTATCAGAGTGATACCAGG50.23992322456813817No Hit
GAAATGAAAGGGTCAGCCATTACTGGACCAGTAGCAAAAGAATGTGCAGA50.23992322456813817No Hit
TGATTTTGTCTCTTTGTTTTAATTTCTCTAATCCTCTGCATGGCTTTCAC50.23992322456813817No Hit
CACAGCCACATAAATAAATGCTTCTCATTTTCATGAAATGATGAAGACAC50.23992322456813817No Hit
GCGAGACCCAGGGACATGCAATTCCCAATTTTTATAAAGGACCCATATAT40.19193857965451055No Hit
TGCAGCACACAGCCAGTACCTGTTTGGACTGAACGTGAGAGCATTGATAG40.19193857965451055No Hit
GCGGTATTTACATATTCATGTACTCGTATAGCATTGGATATAAGACGCTC40.19193857965451055No Hit
GAGAAGGCAACACTCAAAACATCTTTGTTATGACCCTTGAACATCCTGGT40.19193857965451055No Hit
ACTGAATGACGTCTCTCATAAACATATATTTAAACATAAAAAAACTTCAA40.19193857965451055No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC40.19193857965451055No Hit
CCACTGAAAACCAGCTACAACACTGTCCTTGATCTCGTTCAGGTATTGCA40.19193857965451055No Hit
AGTCAATGTCCCCAAAAATGCACTGATATAGATTTATACCGAAATATAAA40.19193857965451055No Hit
CAACTCTCTCATCACTTTCTTCAATGGATTTCTTATGTTCTGGAACAAGT40.19193857965451055No Hit
GGACTCTTCTTTTGTCTGATTTTGCACTATTTTTGAAAAATATCAAACAA40.19193857965451055No Hit
CAAAGGTGTCTATATTAAAGGTGTCCAAAGGCGTCTATATGGTGGCTGTA40.19193857965451055No Hit
GTCCGATGTTCCCTTTGCAGGATCAGCGTATTCCAAATCATCGTAATCAT40.19193857965451055No Hit
GTCACTTCTCAAATTTTGAGAATTATCTGTGTTCACATAGTATTCCTATG40.19193857965451055No Hit
TTTTTTTTTATGATGTAATAATTTAATAATCCAAATTTAATATACAAAGG40.19193857965451055No Hit
CACAGGGCAATCACAATAGATCAACCTTATCTTTGCTAAGATGAGCTTAA40.19193857965451055No Hit
TTTTTTTATCATCCATTAGCATTGACATTATATGGGTATGTTGGAGTCTG40.19193857965451055No Hit
ATCCATTACCCCGAAAGCAATTCTTAGGGTAAAAAGGTAAGACATATAAA40.19193857965451055No Hit
TTTTACTGTGCACAAAAAGAACTGAGATCCGTTGGTATTGGGACCTGCAT40.19193857965451055No Hit
CGATTAATAACGTGAAACTATTATATTCCTAGTAGTTGTTTCTGATACGC40.19193857965451055No Hit
TTACAGTCTAAAATGAGATCATTGAATGACAACTGTGCGTTTGTTCAACT40.19193857965451055No Hit
CCCATTTCTATGCTTTCGAAGTGAATTAAACTGTATTTAGCAAAAGCCTG40.19193857965451055No Hit
GCCATCTGTAATTCCTTCTTCACTGTGCTTCCAAATCTGGGACTGTTCTC40.19193857965451055No Hit
CTGATGCCCAATTACCTTTAACCTTATCCCATACTCCCTTCTGTACATCT40.19193857965451055No Hit
GGTTTAAAGCGCAGGTTTTAGTTGTAAAACAACAAATTTTGGACATTCAT40.19193857965451055No Hit
AAAAAGTTGCAGTTGCCGGCATAACAACAAATACAAACAAGAGGGCCAAG40.19193857965451055No Hit
GGTAAGTCTGTGAAGAGTTCCGCGCTCCTCGAGTTTGTGAATCACTTCAT40.19193857965451055No Hit
ATTTTTTAAAGTATCATATGAATATAAAGTGAAGCACATTGCTCAGTGGT40.19193857965451055No Hit
TTTTTTTTAATCCAACTTTAATTATTTACCTTTTCTGACACCCCTAATAA40.19193857965451055No Hit
CAGCAATGAAATTATTTTCACATTCATCAGTAAGTATGTTATCACTGCAG30.14395393474088292No Hit
TTTATATGAAACCATACTAACATTTTAGTCAAATATCAAAACAGAGCTTC30.14395393474088292No Hit
GTGGATGCGAAAGGTCCAAATTGTCCGGCGAAGCACAGGATATATGCATA30.14395393474088292No Hit
CTTCACCTACCTCTTCTTTCATTTCTTCAACACCCTTTTTAGGTCGTCCT30.14395393474088292No Hit
AATAATGAAACCCTGCAAATATAGCAAATACTGCCCCTATACTTAAGACA30.14395393474088292No Hit
CCCACCATTAGACTTAATAAATTAGGTATGAAAATCAATAAATTTATAGA30.14395393474088292No Hit
TAAAAATAAATTTCTACAATAACTGAAAAAGCACTTGACATGCATTTTAA30.14395393474088292No Hit
CAGCACCGAAACCGGATCCAGAGCCGCCACCGAACCCTGAGCCGGAACCA30.14395393474088292No Hit
GTTAGGTTCATAGACACCTATCTGTTTGTTTATATATATTACTATATTCT30.14395393474088292No Hit
CAACATATTGCACGTCTCTAAGGAGCCTTTTGACATTTTTTATTTTTGCG30.14395393474088292No Hit
TTTCTTCTTGTTTTTCCTGTCTTTACTATTTTGAACACTTCATTTTCACT30.14395393474088292No Hit
TAAACATCATGAAGGGGATAGATTCCCTGGCATGCTTTCTCAATGTCCTT30.14395393474088292No Hit
CAAGTGCGAATTGTATAAACCATATAACTCATTAAGTAACCAATGTAAAA30.14395393474088292No Hit
TGTCTATCTTCGGTAGTGGATCACTAACTCCACGTACAAAATCACTGTCC30.14395393474088292No Hit
GTCATCACAAAAAATACAGAGCCATACACCCCATCCGAAATAGAAAAAGA30.14395393474088292No Hit
GCACTCTTTATTTTTCTTCAAAACAACTTCAGCATATTTCACAAATGCAA30.14395393474088292No Hit
GCCGAAAGGTCTTCCTCCATCTTGGCCTTTGCTTCTTCTACATCCCTTAA30.14395393474088292No Hit
CGATCATAGGTCGGTCAAAAATCTTTTTTTTTGGAAAGTTTGCCAGTTAC30.14395393474088292No Hit
GTTCTTTTTTGGTGTGGTGTAATTCTTCTTCTTACGTTTCTTGGCACCAC30.14395393474088292No Hit
AGAAATCCTACCGTGTTGAAGGTTAATTCGTTCTTTGTTTTCATCAATTA30.14395393474088292No Hit
ATTTCCTACAACAGAAACATTTTCGACAATTGAGCACACATCTGAATTAT30.14395393474088292No Hit
CGTAGATTGGTACGGTGTGGGTTACACCATCTCCGGAGTCGAGTACGATA30.14395393474088292No Hit
ATTATATTCTATAGATATGACAATAATGATATGTTCCTTGACGAGAAATC30.14395393474088292No Hit
GTGTAAAGTACCATCTAGTAAATCTAACATGTAATGTACAATTTAGTAAA30.14395393474088292No Hit
CTTGGGTGCTTGTAACATGTCCCTTCTCTATTTCCGTAGCAAGACCATCT30.14395393474088292No Hit
ATTTGTTTTCGCTTGTTGTGTTAGAAGCCCGTGACCCTCTTGATTCGCAT30.14395393474088292No Hit
GAACGGCGTCCACCTTTAGACCTAGCGAGGGTAAAGTCCAACAATGCATC30.14395393474088292No Hit
CACAAACTCTCCAACAATTCGTTACTACTCCTATTACGACACCTCAAACC30.14395393474088292No Hit
CAAAACACCTCTCATCAGCATTGTGGAATCCTGCCGGCCATACCTGACCA30.14395393474088292No Hit
GTACCGTCCATGACAATGGTTCTCACTGTATTTTCACCTAAATGTTGTGC30.14395393474088292No Hit
CGACCTATCAGCAGAATGTCTGAGGACAATCGTTAAACGTCACCAGTACC30.14395393474088292No Hit
AGATAGTGCACGTGGAAGCAAAGCAACGACAGGACGTACACTGACACGGG30.14395393474088292No Hit
GTTTTAGTGGCCTCTTCCAACTTGAGGGTCGCTTCTTCCAGTTTGGTATT30.14395393474088292No Hit
CGCAGAAAAGGCCGGAGTAGGTGTAGATAAAGCCACTAAAGTATCTGGAC30.14395393474088292No Hit
CGAAATTGTTTTGTGAATTTACGTGACGATGAAATGGCGAAGAATTGACT30.14395393474088292No Hit
TGTCATTCAAAGTGAAATGTAGTGATATAATTGGCTGATATAGTAAATGT30.14395393474088292No Hit
TTCAAATTTAAATTTGAAAGTTTGTATCTCCTGATAGACAATATATCATT30.14395393474088292No Hit
CAACCTGCACCAATAGACTTGTAAAGGGTTTGGTGAGGGCCTTCAGTAGT30.14395393474088292No Hit
CCTTCATATTCGCCGATGCAACAATCCAGCGTTTGTGGTTGCCCCTGGCA30.14395393474088292No Hit
GCAATGGTGACGTCACGTAAATGATACATTATGGGATCAATGGGAACAAT30.14395393474088292No Hit
TTTTTTTTTACAATCACATTTTTTTTATTAAATGATTCATATTCTCTTAT30.14395393474088292No Hit
TGCGACACCTGGTAACCGTAGTTACATCACCACGTGATCACATTGATTTT30.14395393474088292No Hit
GTTGTGTATGTGAAATTCCCCTGAACCGTATGTTGCTTAGTGTAATTCCC30.14395393474088292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14395393474088292No Hit
TATACCTCGTTCTCAAAATATTTAAGCCTCGTTTTCAAAAATGAAACGAA30.14395393474088292No Hit
CGCAATCTATCCCATAATATTAATTACATAAGCTGTGTTATGTTTACACT30.14395393474088292No Hit
TAACCAAACCACGGGGAATAAAGTTCACAAACTCTCCAACAATTCGTTAC30.14395393474088292No Hit
GAGTTCGTTAAATAGTATAATAATAGATCGAATAACTTAGAAGCATTTCC30.14395393474088292No Hit
ACAAAATGTGTATACATGATTGAATAGCTAATTCCATACATAAGAGCGTT30.14395393474088292No Hit
AATGACCTTTATTTACTTCAGTGAAATTTGTTTTCATGTTTGTTGTACAA30.14395393474088292No Hit
GGGTTCCTGGGGTGCTGGGAGCAGAGGTTGTACCCTGGGGCTCCAGGCAA30.14395393474088292No Hit
TTATTTTTAGTAAGTCCTGAATAATCACTTGGCAATTCCCTCATAAGATT30.14395393474088292No Hit
ATGAAGGACCTTGATTGGTCCAACTGGAGCAGACGGCACAGTGTATGGGC30.14395393474088292No Hit
CAGCAGTCCACACCCTCCATCTTCTCGGTTTTTTACCAACTCGGCACCCC30.14395393474088292No Hit
ATTTAAGTTTGTCATCACCAGATATGTCATAGCCGTTAAAACACGTGATT30.14395393474088292No Hit
CAGTCTTCTCGAGGGCAAGGGCAGCCTCCATGGCTTCCAGTCCAGAGCCC30.14395393474088292No Hit

[OK]Adapter Content

Adapter graph