FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-heart-RNA-2_S10_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-2_S10_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC411.0829371368198626No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT70.184891706286318Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG60.15847860538827258Illumina Single End PCR Primer 1 (100% over 50bp)
AAAACTTCCAAATTATTGAATTTAAATGTGTTTAGAACATTGACAACCAT60.15847860538827258No Hit
GTTGACTTTGCTTCAGCTGGATATTACAGATGTTTTAAGAAGAAGTCTTG50.13206550449022716No Hit
TTTAAATTAAAACATTTCGCGAAAGCATAATATCGATCAGTTTGATAAAT50.13206550449022716No Hit
CTCCCAGTCAGCAGTTTGTCAATGGCATAAACCTAGGATACAAAGTGCTG50.13206550449022716No Hit
GGAAGAAGAACCGCAACAAATTCCGTCAGATGCGGACGAATAGTTACAAT50.13206550449022716No Hit
CTTTAATCTAAATTGTGATTTGTCTAGCTATTGTCTGAACACAAGATTGT50.13206550449022716No Hit
GTCGGTTCACTGGGGGCAGATTTGCTGGCAGAAAGGCTGTCATTGTAAAG40.10565240359218173No Hit
TATAAACGCCCCTCGCTGGATAAAAGGTTTCAACGGCAACGAGCTTCAGC40.10565240359218173No Hit
CTTTTGTTTTACATTTTTAAGATACATTGAGATATAAAATGGTACATTTA40.10565240359218173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.10565240359218173No Hit
GTGAAATCGTCAGAGACATCAAGGAGAAGCTCTGCTATGTTGCCCTGGAC40.10565240359218173No Hit
GTACATCCCCGACCTAGAAAAATTATGCAGCTGTTCCGACTCCGTCAGAT40.10565240359218173No Hit
CATTTCTCTAACAAAGGCTATAAAAGTTATGAATTGAATTAAATACATAT40.10565240359218173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)