FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-heart-RNA-2_S10_L002_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-heart-RNA-2_S10_L002_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681
Sequences flagged as poor quality0
Sequence length42-151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC391.454681089145841No Hit
AAAACTTCCAAATTATTGAATTTAAATGTGTTTAGAACATTGACAACCAT60.2237970906378217No Hit
CTCCCAGTCAGCAGTTTGTCAATGGCATAAACCTAGGATACAAAGTGCTG50.18649757553151808No Hit
GTTGACTTTGCTTCAGCTGGATATTACAGATGTTTTAAGAAGAAGTCTTG50.18649757553151808No Hit
GGAAGAAGAACCGCAACAAATTCCGTCAGATGCGGACGAATAGTTACAAT50.18649757553151808No Hit
CTTTAATCTAAATTGTGATTTGTCTAGCTATTGTCTGAACACAAGATTGT50.18649757553151808No Hit
CTTTTGTTTTACATTTTTAAGATACATTGAGATATAAAATGGTACATTTA40.14919806042521447No Hit
GTCGGTTCACTGGGGGCAGATTTGCTGGCAGAAAGGCTGTCATTGTAAAG40.14919806042521447No Hit
GTGAAATCGTCAGAGACATCAAGGAGAAGCTCTGCTATGTTGCCCTGGAC40.14919806042521447No Hit
GTACATCCCCGACCTAGAAAAATTATGCAGCTGTTCCGACTCCGTCAGAT40.14919806042521447No Hit
TTTAAATTAAAACATTTCGCGAAAGCATAATATCGATCAGTTTGATAAAT40.14919806042521447No Hit
TATAAACGCCCCTCGCTGGATAAAAGGTTTCAACGGCAACGAGCTTCAGC40.14919806042521447No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT40.14919806042521447Illumina Single End PCR Primer 1 (100% over 50bp)
CATTTCTCTAACAAAGGCTATAAAAGTTATGAATTGAATTAAATACATAT40.14919806042521447No Hit
TTCAGTTGGAAGAGTATATAGGCGCATCTTTTTCTATTTCGGATGGGGTG30.11189854531891084No Hit
GAGAACACAGATGAGACTTACTGCATTGACAACGAGGCTCTCTACGATAT30.11189854531891084No Hit
GGTATTATGATTTTCAGTATGAACGCTGATGATTATTTGGGGAGCTGTGG30.11189854531891084No Hit
CCTGGATACAAGACGCAACATCGTTTCTCTACCAATTAAATTATAATGGC30.11189854531891084No Hit
TGAGGGTACCTGTCTCGGAAGACATGTTGGGACGTGTGTTTAATGGGTCT30.11189854531891084No Hit
TCCAGCCATTCATATAGCATCTTGGACAACAAAACCCTGCAGCAACTGAT30.11189854531891084No Hit
CAGATCCTGCCAGTCAGCATTCAAAACAAAGTGATCTTTGACACAAGCTA30.11189854531891084No Hit
CAGAATGGCGTGGTCAGGTATGGCCGGCAGGATTCCACAATGCTGATGAG30.11189854531891084No Hit
AGACTTTCTGGGGATTTCATTAGCAGGGTGAATCTTCTGCTTCATATTTC30.11189854531891084No Hit
GTCCTTGACCCTTAACTGCTAGGTGTGGTGGGTTAAGAATAGCTGGGTCT30.11189854531891084No Hit
CCGGACTGGGCGGGAAGCCAGGCACATGTGAGATATGTCCGGCAGCCGGG30.11189854531891084No Hit
TGTCTACCAACGTGTGTGTGTGTCAACCAGAATTATCTAGATGAACCCTT30.11189854531891084No Hit
TCTTTTTAAAAACGTACACAATTATTGAGAATTATCACTATAGATAACAC30.11189854531891084No Hit
GGACAATACATGTCACCTATACCATCACTAGAGCACCATTGTACACATGG30.11189854531891084No Hit
AAAGGGTCACAAGTTCAACCAAACAACTGGTGGATCTCGCAGGACCCACT30.11189854531891084No Hit
GATGTCTTCTCAAAGTCTGAACTTGATTACTGTCGCAATTCTGTGTGCAT30.11189854531891084No Hit
TTTATAACAAAAGAAATTGCAATAAACTGGGCTCAAGTAAGAGTCATCCG30.11189854531891084No Hit
CTTTGTTATTAGTGTTAGGGTGGGGTTATCAACCAGAGCGGTTGCAGGCT30.11189854531891084No Hit
TTATAACACATGACAAAAAAAATTAATTTTTATAAGCCGTGTCCGTTTTA30.11189854531891084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)