FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-7_S49_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-7_S49_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG60926.420824295010846Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2048.850325379609544Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100.43383947939262474No Hit
GTGAAAGCCAAGATCCAAGACAAAGAAGGAATTCCCCCAGATCAGCAGAG80.3470715835140998No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA50.21691973969631237Illumina Single End PCR Primer 1 (100% over 50bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG40.1735357917570499Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGACTGTAGATCTCGGTGGTCGCCG40.1735357917570499Illumina Single End PCR Primer 1 (98% over 50bp)
ACGGATTCCTCTAGTACCCGGATGGTCGATGGGAACTGTAACATCTACAA40.1735357917570499No Hit
CAGACGCCGGTGTCACTCCTCTTGTTGATCTGCTGCTCAAGGCCGGTGAG40.1735357917570499No Hit
GTAGTGGTAACAGTCTCGGTGAGTGAAAACGTAATTACTCCCTTTGAGTG30.1301518438177874No Hit
CTTACATATGTACATAGCGTGATATTTTTTTTTAAATTACGGGGAATCCT30.1301518438177874No Hit
GTCAGGTATAAAACAACTTGATGATAAGATCGTTGACCTTAATGCCAAGA30.1301518438177874No Hit
CTTTGCTATTTGAGCTGGTATAGTTGGTACAGCTTTTAGAATATTAATTC30.1301518438177874No Hit
TCAATGTGCACCAAGTATCCGTTCCCGGATGGGTTCTACTACCTCGCTGG30.1301518438177874No Hit
CAAGGTTGATAGAAAGAGCTTTTTTGGGTTAATAGGATGTAAATTCAGGT30.1301518438177874No Hit
CTCCCACAAAAAATTATTCGCTCCTGACATGCATTTCCTGTGTTTTTTTC30.1301518438177874No Hit
TTTGAGAAGGAGGCACAGGAGATGGGAAAAGGATCCTTCAAATACGCTTG30.1301518438177874No Hit
GGAGAATTGATGATGTACATTTCAAAGATGGTGCCGACATCAGACAAGGG30.1301518438177874No Hit
TCAAGATGGTCACGGGCTTCTAACACAACAAGCGAAAACAAATATTTACG30.1301518438177874No Hit
AAATTACCTCAGGTCGAGAATTAAATAAAGGGGCACTATAGATAACAAAC30.1301518438177874No Hit
GTTTTTTGTACTACATTATTTAATTCCTTTTGTTGTGAGGGGTTTAGCGG30.1301518438177874No Hit
AGCATGTGTTAGAATTGTGTCTGTCAAATAAAAGTGTGAGAATGAAGACA30.1301518438177874No Hit
TGGTACTGGGTGAACAGTCTATCCTCCCCTTTCTTCAAATGTTGCCCATA30.1301518438177874No Hit
CTTTAAAACTGTTTGCTAACAAATTGAAAATAATCTTTTATTAAAAAAAC30.1301518438177874No Hit
AGCAAATATAATTGTAGAAAAGTTAGTGAATGATAATGGAAGTTCAGGTT30.1301518438177874No Hit
GATCGGAAGAGAGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG30.1301518438177874Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)