FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-gonad-RNA-7_S49_L007_R2_001.fastp-trim.20220908.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-gonad-RNA-7_S49_L007_R2_001.fastp-trim.20220908.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327
Sequences flagged as poor quality0
Sequence length35-151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG30322.833458929917107Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1067.987942727957799Illumina Single End PCR Primer 1 (100% over 50bp)
GTGAAAGCCAAGATCCAAGACAAAGAAGGAATTCCCCCAGATCAGCAGAG80.6028636021100227No Hit
ACGGATTCCTCTAGTACCCGGATGGTCGATGGGAACTGTAACATCTACAA40.30143180105501133No Hit
CAGACGCCGGTGTCACTCCTCTTGTTGATCTGCTGCTCAAGGCCGGTGAG40.30143180105501133No Hit
GTAGTGGTAACAGTCTCGGTGAGTGAAAACGTAATTACTCCCTTTGAGTG30.22607385079125847No Hit
CTTACATATGTACATAGCGTGATATTTTTTTTTAAATTACGGGGAATCCT30.22607385079125847No Hit
GTCAGGTATAAAACAACTTGATGATAAGATCGTTGACCTTAATGCCAAGA30.22607385079125847No Hit
CTTTGCTATTTGAGCTGGTATAGTTGGTACAGCTTTTAGAATATTAATTC30.22607385079125847No Hit
TCAATGTGCACCAAGTATCCGTTCCCGGATGGGTTCTACTACCTCGCTGG30.22607385079125847No Hit
CAAGGTTGATAGAAAGAGCTTTTTTGGGTTAATAGGATGTAAATTCAGGT30.22607385079125847No Hit
CTCCCACAAAAAATTATTCGCTCCTGACATGCATTTCCTGTGTTTTTTTC30.22607385079125847No Hit
TTTGAGAAGGAGGCACAGGAGATGGGAAAAGGATCCTTCAAATACGCTTG30.22607385079125847No Hit
GGAGAATTGATGATGTACATTTCAAAGATGGTGCCGACATCAGACAAGGG30.22607385079125847No Hit
TCAAGATGGTCACGGGCTTCTAACACAACAAGCGAAAACAAATATTTACG30.22607385079125847No Hit
AAATTACCTCAGGTCGAGAATTAAATAAAGGGGCACTATAGATAACAAAC30.22607385079125847No Hit
GTTTTTTGTACTACATTATTTAATTCCTTTTGTTGTGAGGGGTTTAGCGG30.22607385079125847No Hit
AGCATGTGTTAGAATTGTGTCTGTCAAATAAAAGTGTGAGAATGAAGACA30.22607385079125847No Hit
TGGTACTGGGTGAACAGTCTATCCTCCCCTTTCTTCAAATGTTGCCCATA30.22607385079125847No Hit
CTTTAAAACTGTTTGCTAACAAATTGAAAATAATCTTTTATTAAAAAAAC30.22607385079125847No Hit
AGCAAATATAATTGTAGAAAAGTTAGTGAATGATAATGGAAGTTCAGGTT30.22607385079125847No Hit
GATCGGAAGAGAGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG30.22607385079125847Illumina Single End PCR Primer 1 (98% over 50bp)
ATCTGTGCATCACCATGGGTATCAGCAATCTTGTGCAGGTCCAATAATGC20.15071590052750566No Hit
TTCATAAACTCTTTCTTCCTTGCACCTGAAATCGTCGCCGGCATGGAGAC20.15071590052750566No Hit
AATTGACATATATATATATTACCACATGGCATGAGAACTCACAATTTTTA20.15071590052750566No Hit
GTTTGAGGTGTCGTAATAGGAGTAGTAACGAATTGTTGGAGAGTTTGTGA20.15071590052750566No Hit
TGCAAGTGTAGAACGCTTGGGATATTTTTAGTTTGTCAAAAGTGTGGATA20.15071590052750566No Hit
CTACACGTTCAGAGTTCTACAGTCCGACGATCGTCGGACTGTAG20.15071590052750566Illumina RNA PCR Primer (100% over 27bp)
CAGCAGTTTCCTTGTATGGGTTGCTGAACCTGAACACGACGACAGAAAAA20.15071590052750566No Hit
GGGAATGGAATCTGCTGGTATCCACGAAACCACATACAACAGCATCATGA20.15071590052750566No Hit
AGAAGAATGCCAGACGATTCCAAGATACCTGTCGCGAGATGCAGAGTACG20.15071590052750566No Hit
CTTCTATTTTGGGGGCTATTAATTTCATTTCTACAACTGTGAATATACGG20.15071590052750566No Hit
TTCAACTTTACTTAGAAGTATTAGAGATGAATGAATTTAATTGTGTACAT20.15071590052750566No Hit
GTCCAGTTCGATGATAATTTACCACCTATGTTAAATGCTTTGGAAGTAGA20.15071590052750566No Hit
GGGGACGATGCCCCGAGGGCCGTGTTCCCCTCCATCGTCGGCAGACCCAG20.15071590052750566No Hit
CACTTGTACAGTAACTACTGTCAAAACATATGCTCACATTAAATAATTTA20.15071590052750566No Hit
GTGAAGGGTCAAACATCAAAGGTCGAGAAGCAGGAGAAGAAGAAGAGGAA20.15071590052750566No Hit
GTGAGGGTTGGGGTACTTTTATACGAATATTACTATCGAAAATGACCCAA20.15071590052750566No Hit
TCTGGGCCCTGGCTCAGAGACACCCCAGGCCCAGA20.15071590052750566No Hit
TGGACTTGCTGAGCAATACCAACATGGTAGATTTTGCCATGGATGTACAC20.15071590052750566No Hit
GTAAAACCAGTCAAAGAAGAGCTTGATGTTAACGCAACTGAAAAACAATG20.15071590052750566No Hit
TGGAGCCTGGGATCGAACCCACGACATTTGAGATGTGTGGCTGATAGTCC20.15071590052750566No Hit
TTTTCCTCACCACCACTGGGTTCACCATTTGACACAGGCACTTCTTCAGT20.15071590052750566No Hit
ATTTTTTAGTTCCTTTGATACTTTCTGCTCCTGATATGGCATTCCCTCGA20.15071590052750566No Hit
TTATAATGTATTTTTTTCCCTTTTATTTTGCCAGACAGTACATATTTAAG20.15071590052750566No Hit
GCTCGACATCTCCCATGTATTTAGACCGCAGGTTTTAT20.15071590052750566No Hit
TATAAATTTATTGATTTTCATACCTAATTTATTAAGTCTAATGGTGGGAT20.15071590052750566No Hit
TGACATTCATATCTCCCGTCTCATTGATTGTGATATTTTACAGGTAGTCG20.15071590052750566No Hit
AACACGTAGTGCCGAAGTATCCTTAAGACCGTGTTACTAACTATACTTTC20.15071590052750566No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGACTGTAGATCTCGGTGGTCGCCG20.15071590052750566Illumina Single End PCR Primer 1 (98% over 50bp)
TTTGATTCCTATATGGTACCAAGATCAGATTTAGGAGGAAGGAATGAAGT20.15071590052750566No Hit
CGTGAACTTCTCTTATGTCTTTTAAAGAATTTATAATTCGGTGATATCGT20.15071590052750566No Hit
TGAATTTTCATTAAGATAAGCTCCAAGAGACCTCCAATTAAACAAATTAT20.15071590052750566No Hit
CACGGAGTTACCAGTAAGACACCAGAAATGTCAACGGTAGTTAATATCTT20.15071590052750566No Hit
GTTTTTTAAAAAGCGCAGTGCTTTATGGGGCTTGGTGGTTACAGTAGTTC20.15071590052750566No Hit
TGGCTGTACATCAGCAAATGATTGCAAATGATTATTATTATCAAAATAAT20.15071590052750566No Hit
TGGCATGTGAGCGGACAGACACATTTGTCAAATATCACAGCTATGGCGTC20.15071590052750566No Hit
GTGATATCAGGTCTTATGATACATACAAATATACTACATACCTATGCTTT20.15071590052750566No Hit
AGATGACATGATATTTGATGAAGAGCCAGCATCTCTTTGTACAGAAATAC20.15071590052750566No Hit
GGCAGAGATCTTACAGATTACTTGATGAAGATCCTTACCGAGCGTGGTTA20.15071590052750566No Hit
GTGATTTTGGTATCATTATTTTCTTTTCTTTCATAGTAAGTCTATTCACA20.15071590052750566No Hit
GTCGGAAGTTTTCTTTTTTGTCTCATTTTTTTGGTCCTTTTTTCATTTTA20.15071590052750566No Hit
TGCAAAAGTTAGCAGCGGCATCTCCAGTGTTAAAGCAGGCTAAACGGATT20.15071590052750566No Hit
TTGTTCTCAGTTTTCCCCGATAAAAAGTGAGGTTTCCAAGAGTATATGAAGTAAGTTTCTAGGATAT20.15071590052750566No Hit
GTTTTCATTATTTTTATACTTATTTTGTAATTTAAGTGGATGTATTGATG20.15071590052750566No Hit
CCTCATTAAACTTCCATAATGCACATTTTTTGATAATAACAGAAATGATT20.15071590052750566No Hit
GTTTTGAGATATAAATCATATAAATAATCAGGATTTGGTTATATACAATT20.15071590052750566No Hit
GATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG20.15071590052750566Illumina Single End PCR Primer 1 (98% over 50bp)
GTTTTTTTTCCACCAATAGACTCTCTTTAAAGGATATTTAATACACATAA20.15071590052750566No Hit
GCCAGGTCCTGTCTATCGCAGATACATGTATATCCAGAGAAGTTTTCAAC20.15071590052750566No Hit
GGATTCCGGAGATGGTGTTTCACATACTGTACCAATCTACGAAGGTTATG20.15071590052750566No Hit
CAACCCAACATTGGCCACCGTCGAGAAACACGGAGGCACCCAAAAGATGG20.15071590052750566No Hit
CAAAAGTGCTATTAAAACAACAATTAAAAAGAATCATTACTCATTGGTTT20.15071590052750566No Hit
TTTGTCTCATTTTTTTGGTCCTTTTTTCATTTTAGGTTAGGATCTTTGGC20.15071590052750566No Hit
TGGAAGCGAAATTGGAGCGTCAGGAATCGACGACACCATGGGGATTTCGC20.15071590052750566No Hit
AAACCATTAAAATTGTACTATAATCATCTGCTTTAATTGTGTATCTCACA20.15071590052750566No Hit
GCGCACCCTGGTGGAAGTTGAAACTCGCTACAAGACCGAGATCTCCCGCA20.15071590052750566No Hit
CCATAAACTGCCTACACTTGGCACCTTTTTACACTACTAAGGTGAATTAT20.15071590052750566No Hit
TCACCCTCAAATCTCAACCCCTTGAAGAAATGACCGGGCCTTTTTCCAAA20.15071590052750566No Hit
TCTAAAGACTCTTATTGCAGCGATACGTATAACAAATTATCAAAGATTTA20.15071590052750566No Hit
CTAAAATATAATTTGACACAGCAGATGATAATGGAAGAAAATTATGTTGT20.15071590052750566No Hit
GGCGTTTTGGTGCTGAATAATAACTTTTTGTTAGTCCTGAGAGTTTTAAT20.15071590052750566No Hit
TTTAATTTCAAACTCATGATTACTTAATAAATGTTTTTGAAAGCATAAAC20.15071590052750566No Hit
CAGGCACAGATAGATAAATTAAAAGAAAATGAAGAATCTCAGACACAAAC20.15071590052750566No Hit
ATCAACCACTCTGCGTCACACATCTCGAGCCAAGACTTCAGATGAAACCA20.15071590052750566No Hit
CAAAACTTAGACATAAACTCTACATTGCAGCCTAAAATTCAAACAAATTG20.15071590052750566No Hit
TGAACACGGTATCGTCACAAACTGGGACGATATGGAGAAGATCTGGCATC20.15071590052750566No Hit
TATCTCTCCAGTTTATATTATAACTCTCATCTGGTATCACTCCTGTTTAT20.15071590052750566No Hit
AGTCCACGACTCAAAATTATTAAAGGTTTTGCTGTGAACTGAATAGAATG20.15071590052750566No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT20.15071590052750566Illumina Single End PCR Primer 1 (100% over 50bp)
CTACGCCACCTGTCTCCATGGCTGACTACAGGTCACATGACACATCAGAT20.15071590052750566No Hit
TTTTAAGACAGGTTGTTGTTTAATGCAGATTATTTGCTCTGAAAGCATAT20.15071590052750566No Hit
GGAATCGTCACACACAAGGACGGATCTGGTGCAGCAGGCAAATGATGATA20.15071590052750566No Hit
TCCACATTCAACCAGAGTGGTATTTCTTATTTGCTTACACGATCTTGCGG20.15071590052750566No Hit
GTCAAAATAAAGAAAAGTAAGATAGTTGGGCTTCAATAAGTATTCATACG20.15071590052750566No Hit
CAGCAACTGCATACTTTTAATATGCAAGCCATCACGCCAGATCAAAATAC20.15071590052750566No Hit
CTTTGTTTGGGTTCCAATTCTGATAAAATATAATATTACTGGTAAGTGTG20.15071590052750566No Hit
CGGCGGTATTCTGATTATCCTGATGCATATTTCTTTTATAATATAGTTTC20.15071590052750566No Hit
GATTGCTGTGGAAGGTCCGTCCAAGGCTGAGGTAGACTTTGAAGACAGGA20.15071590052750566No Hit
CCTGTCACCGCCATCATCTCCGGATCTCTACCAGGAATCGACACAAAAAC20.15071590052750566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)